TY - JOUR A1 - Akimbekov, Nuraly A1 - Digel, Ilya A1 - Abdieva, Gulzhamal A1 - Ualieva, Perizat A1 - Tastambek, Kuanysh T1 - Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data JF - Biofuels N2 - The vast metabolic potential of microbes in brown coal (lignite) processing and utilization can greatly contribute to innovative approaches to sustainable production of high-value products from coal. In this study, the multi-faceted and complex coal biosolubilization process by Bacillus sp. RKB 7 isolate from the Kazakhstan coal-mining soil is reported, and the derived products are characterized. Lignite solubilization tests performed for surface and suspension cultures testify to the formation of numerous soluble lignite-derived substances. Almost 24% of crude lignite (5% w/v) was solubilized within 14 days under slightly alkaline conditions (pH 8.2). FTIR analysis revealed various functional groups in the obtained biosolubilization products. Analyses of the lignite-derived humic products by UV-Vis and fluorescence spectrometry as well as elemental analysis yielded compatible results indicating the emerging products had a lower molecular weight and degree of aromaticity. Furthermore, XRD and SEM analyses were used to evaluate the biosolubilization processes from mineralogical and microscopic points of view. The findings not only contribute to a deeper understanding of microbe–mineral interactions in coal environments, but also contribute to knowledge of coal biosolubilization and bioconversion with regard to sustainable production of humic substances. The detailed and comprehensive analyses demonstrate the huge biotechnological potential of Bacillus sp. for agricultural productivity and environmental health. KW - humic acid KW - Bacillus sp KW - lignite KW - Biosolubilization Y1 - 2021 SN - 1759-7277 VL - 12 IS - 3 SP - 247 EP - 258 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Albanna, Walid A1 - Conzen, Catharina A1 - Weiss, Miriam A1 - Seyfried, Katharina A1 - Kotliar, Konstantin A1 - Schmidt, Tobias Philip A1 - Kuerten, David A1 - Hescheler, Jürgen A1 - Bruecken, Anne A1 - Schmidt-Trucksäss, Arno A1 - Neumaier, Felix A1 - Wiesmann, Martin A1 - Clusmann, Hans A1 - Schubert, Gerrit Alexander T1 - Non-invasive assessment of neurovascular coupling after aneurysmal subarachnoid hemorrhage: a prospective observational trial using retinal vessel analysis JF - Frontiers in Neurology N2 - Delayed cerebral ischemia (DCI) is a common complication after aneurysmal subarachnoid hemorrhage (aSAH) and can lead to infarction and poor clinical outcome. The underlying mechanisms are still incompletely understood, but animal models indicate that vasoactive metabolites and inflammatory cytokines produced within the subarachnoid space may progressively impair and partially invert neurovascular coupling (NVC) in the brain. Because cerebral and retinal microvasculature are governed by comparable regulatory mechanisms and may be connected by perivascular pathways, retinal vascular changes are increasingly recognized as a potential surrogate for altered NVC in the brain. Here, we used non-invasive retinal vessel analysis (RVA) to assess microvascular function in aSAH patients at different times after the ictus. Y1 - 2021 U6 - http://dx.doi.org/10.3389/fneur.2021.690183 SN - 1664-2295 VL - 12 IS - 12 SP - 1 EP - 15 ER - TY - JOUR A1 - Alexyuk, Madina A1 - Bogoyavlenskiy, Andrey A1 - Alexyuk, Pavel A1 - Moldakhanov, Yergali A1 - Berezin, Vladimir A1 - Digel, Ilya T1 - Epipelagic microbiome of the Small Aral Sea: Metagenomic structure and ecological diversity JF - MicrobiologyOpen N2 - Microbial diversity studies regarding the aquatic communities that experienced or are experiencing environmental problems are essential for the comprehension of the remediation dynamics. In this pilot study, we present data on the phylogenetic and ecological structure of microorganisms from epipelagic water samples collected in the Small Aral Sea (SAS). The raw data were generated by massive parallel sequencing using the shotgun approach. As expected, most of the identified DNA sequences belonged to Terrabacteria and Actinobacteria (40% and 37% of the total reads, respectively). The occurrence of Deinococcus-Thermus, Armatimonadetes, Chloroflexi in the epipelagic SAS waters was less anticipated. Surprising was also the detection of sequences, which are characteristic for strict anaerobes—Ignavibacteria, hydrogen-oxidizing bacteria, and archaeal methanogenic species. We suppose that the observed very broad range of phylogenetic and ecological features displayed by the SAS reads demonstrates a more intensive mixing of water masses originating from diverse ecological niches of the Aral-Syr Darya River basin than presumed before. KW - ecological structure KW - metagenomics KW - microbial diversity KW - shotgun sequencing KW - Small Aral Sea Y1 - 2021 U6 - http://dx.doi.org/10.1002/mbo3.1142 SN - 2045-8827 VL - 10 IS - 1 SP - 1 EP - 10 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Ball, Christopher Stephen A1 - Vögele, Stefan A1 - Grajewski, Matthias A1 - Kuckshinrichs, Wilhelm T1 - E-mobility from a multi-actor point of view: Uncertainties and their impacts JF - Technological Forecasting and Social Change Y1 - 2021 SN - 0040-1625 U6 - http://dx.doi.org/10.1016/j.techfore.2021.120925 VL - 170 IS - Art. 120925 PB - Elsevier CY - Amsterdam ER - TY - THES A1 - Bayer, Robin T1 - Development of a novel in-vitro vascular model for determination of physiological and pathophysiological mechanobiology Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:38-362212 N1 - Dissertation, Universität zu Köln, 2021 PB - Universität zu Köln CY - Köln ER - TY - CHAP A1 - Bornheim, Tobias A1 - Grieger, Niklas A1 - Bialonski, Stephan T1 - FHAC at GermEval 2021: Identifying German toxic, engaging, and fact-claiming comments with ensemble learning T2 - Proceedings of the GermEval 2021 Workshop on the Identification of Toxic, Engaging, and Fact-Claiming Comments : 17th Conference on Natural Language Processing KONVENS 2021 Y1 - 2021 U6 - http://dx.doi.org/10.48415/2021/fhw5-x128 N1 - SP - 105 EP - 111 PB - Heinrich Heine University CY - Düsseldorf ER - TY - JOUR A1 - Brockhaus, Moritz K. A1 - Behbahani, Mehdi A1 - Muris, Farina A1 - Jansen, Sebastian V. A1 - Schmitz- Rode, Thomas A1 - Steinseifer, Ulrich A1 - Clauser, Johanna C. T1 - In vitro thrombogenicity testing of pulsatile mechanical circulatory support systems: Design and proof-of-concept JF - Artificial Organs N2 - Thrombogenic complications are a main issue in mechanical circulatory support (MCS). There is no validated in vitro method available to quantitatively assess the thrombogenic performance of pulsatile MCS devices under realistic hemodynamic conditions. The aim of this study is to propose a method to evaluate the thrombogenic potential of new designs without the use of complex in-vivo trials. This study presents a novel in vitro method for reproducible thrombogenicity testing of pulsatile MCS systems using low molecular weight heparinized porcine blood. Blood parameters are continuously measured with full blood thromboelastometry (ROTEM; EXTEM, FIBTEM and a custom-made analysis HEPNATEM). Thrombus formation is optically observed after four hours of testing. The results of three experiments are presented each with two parallel loops. The area of thrombus formation inside the MCS device was reproducible. The implantation of a filter inside the loop catches embolizing thrombi without a measurable increase of platelet activation, allowing conclusions of the place of origin of thrombi inside the device. EXTEM and FIBTEM parameters such as clotting velocity (α) and maximum clot firmness (MCF) show a total decrease by around 6% with a characteristic kink after 180 minutes. HEPNATEM α and MCF rise within the first 180 minutes indicate a continuously increasing activation level of coagulation. After 180 minutes, the consumption of clotting factors prevails, resulting in a decrease of α and MCF. With the designed mock loop and the presented protocol we are able to identify thrombogenic hot spots inside a pulsatile pump and characterize their thrombogenic potential. Y1 - 2021 U6 - http://dx.doi.org/10.1111/aor.14046 SN - 1525-1594 VL - 45 IS - 12 SP - 1513 EP - 1521 PB - Wiley CY - Weinheim ER - TY - BOOK A1 - Dikta, Gerhard A1 - Scheer, Marsel T1 - Bootstrap Methods: With Applications in R N2 - This book provides a compact introduction to the bootstrap method. In addition to classical results on point estimation and test theory, multivariate linear regression models and generalized linear models are covered in detail. Special attention is given to the use of bootstrap procedures to perform goodness-of-fit tests to validate model or distributional assumptions. In some cases, new methods are presented here for the first time. The text is motivated by practical examples and the implementations of the corresponding algorithms are always given directly in R in a comprehensible form. Overall, R is given great importance throughout. Each chapter includes a section of exercises and, for the more mathematically inclined readers, concludes with rigorous proofs. The intended audience is graduate students who already have a prior knowledge of probability theory and mathematical statistics. Y1 - 2021 SN - 978-3-030-73480-0 U6 - http://dx.doi.org/10.1007/978-3-030-73480-0 PB - Springer CY - Cham ER - TY - JOUR A1 - Engelmann, Ulrich M. A1 - Shalaby, Ahmed A1 - Shasha, Carolyn A1 - Krishnan, Kannan M. A1 - Krause, Hans-Joachim T1 - Comparative modeling of frequency mixing measurements of magnetic nanoparticles using micromagnetic simulations and Langevin theory JF - Nanomaterials N2 - Dual frequency magnetic excitation of magnetic nanoparticles (MNP) enables enhanced biosensing applications. This was studied from an experimental and theoretical perspective: nonlinear sum-frequency components of MNP exposed to dual-frequency magnetic excitation were measured as a function of static magnetic offset field. The Langevin model in thermodynamic equilibrium was fitted to the experimental data to derive parameters of the lognormal core size distribution. These parameters were subsequently used as inputs for micromagnetic Monte-Carlo (MC)-simulations. From the hysteresis loops obtained from MC-simulations, sum-frequency components were numerically demodulated and compared with both experiment and Langevin model predictions. From the latter, we derived that approximately 90% of the frequency mixing magnetic response signal is generated by the largest 10% of MNP. We therefore suggest that small particles do not contribute to the frequency mixing signal, which is supported by MC-simulation results. Both theoretical approaches describe the experimental signal shapes well, but with notable differences between experiment and micromagnetic simulations. These deviations could result from Brownian relaxations which are, albeit experimentally inhibited, included in MC-simulation, or (yet unconsidered) cluster-effects of MNP, or inaccurately derived input for MC-simulations, because the largest particles dominate the experimental signal but concurrently do not fulfill the precondition of thermodynamic equilibrium required by Langevin theory. KW - Magnetic nanoparticles KW - Frequency mixing magnetic detection KW - Langevin theory KW - Micromagnetic simulation KW - Nonequilibrium dynamics Y1 - 2021 SN - 2079-4991 U6 - http://dx.doi.org/10.3390/nano11051257 N1 - This article belongs to the Special Issue Applications and Properties of Magnetic Nanoparticles VL - 11 IS - 5 SP - 1 EP - 16 PB - MDPI CY - Basel ER - TY - CHAP A1 - Engelmann, Ulrich M. A1 - Shasha, Carolyn A1 - Slabu, Ioana T1 - Magnetic nanoparticle relaxation in biomedical application: focus on simulating nanoparticle heating T2 - Magnetic nanoparticles in human health and medicine Y1 - 2021 SN - 978-1-119-75467-1 SP - 327 EP - 354 PB - Wiley-Blackwell CY - Hoboken, New Jeersey ER -