Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids

  • The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L−1 are reached. However, the glucose yield decreases from 0.81 to 0.72 g g−1 at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49 g g−1. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33 g g−1.

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Author:Nils Tippkötter, Anna-Maria Duwe, Sebastian Wiesen, Tim Sieker, Roland Ulber
Parent Title (English):Bioresource Technology
Document Type:Article
Year of Completion:2014
Date of the Publication (Server):2017/08/18
First Page:447
Last Page:455
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie

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