TY  - CONF
A1  - Raman, Aravind Hariharan
A1  - Jung, Alexander
A1  - Horváth, András
A1  - Becker, Nadine
A1  - Staat, Manfred
A2  - Staat, Manfred
A2  - Erni, Daniel
T1  - Modification of a computer model of human stem cell-derived cardiomyocyte electrophysiology based on Patch-Clamp measurements
T2  - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH Aachen
N2  - Human induced pluripotent stem cells (hiPSCs) have shown to be promising in disease studies and drug screenings [1]. Cardiomyocytes derived from hiPSCs have been extensively investigated using patch-clamping and optical methods to compare their electromechanical behaviour relative to fully matured adult cells. Mathematical models can be used for translating findings on hiPSCCMs to adult cells [2] or to better understand the mechanisms of various ion channels when a drug is applied [3,4]. Paci et al. (2013) [3] developed the first model of hiPSC-CMs, which they later refined based on new data [3]. The model is based on iCells® (Fujifilm Cellular Dynamics, Inc. (FCDI), Madison WI, USA) but major differences among several cell lines and even within a single cell line have been found and motivate an approach for creating sample-specific models. We have developed an optimisation algorithm that parameterises the conductances (in S/F=Siemens/Farad) of the latest Paci et al. model (2018) [5] using current-voltage data obtained in individual patch-clamp experiments derived from an automated patch clamp system (Patchliner, Nanion Technologies GmbH, Munich).
Y1  - 2019
UR  - https://opus.bibliothek.fh-aachen.de/opus4/frontdoor/index/index/docId/8926
SN  - 978-3-940402-22-6
SP  - 10
EP  - 11
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  -