TY  - JOUR
A1  - Haeger, Gerrit
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent
T2  - Analytical Biochemistry
N2  - An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.
Y1  - 2022
UR  - https://opus.bibliothek.fh-aachen.de/opus4/frontdoor/index/index/docId/10514
SN  - 1096-0309
IS  - 624
PB  - Elsevier
CY  - Amsterdam
ER  -