TY  - CONF
A1  - Pijanowska, Dorota G.
A1  - Remiszewska, Elzbieta
T1  - pH-based detection of phenylalnine by potentiometric and colorimetric methods
N2  - In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD+, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration.
KW  - Biosensor
KW  - Phenylalanine determination
KW  - enzymatic methods
KW  - pH-based biosensing
Y1  - 2006
UR  - https://opus.bibliothek.fh-aachen.de/opus4/frontdoor/index/index/docId/123
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:a96-opus-1536
ER  -