TY  - JOUR
A1  - Dantism, Shahriar
A1  - Röhlen, Desiree
A1  - Dahmen, Markus
A1  - Wagner, Torsten
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - LAPS-based monitoring of metabolic responses of bacterial cultures in a paper fermentation broth
T2  - Sensors and Actuators B: Chemical
N2  - As an alternative renewable energy source, methane production in biogas plants is gaining more and more attention. Biomass in a bioreactor contains different types of microorganisms, which should be considered in terms of process-stability control. Metabolically inactive microorganisms within the fermentation process can lead to undesirable, time-consuming and cost-intensive interventions. Hence, monitoring of the cellular metabolism of bacterial populations in a fermentation broth is crucial to improve the biogas production, operation efficiency, and sustainability. In this work, the extracellular acidification of bacteria in a paper-fermentation broth is monitored after glucose uptake, utilizing a differential light-addressable potentiometric sensor (LAPS) system. The LAPS system is loaded with three different model microorganisms (Escherichia coli, Corynebacterium glutamicum, and Lactobacillus brevis) and the effect of the fermentation broth at different process stages on the metabolism of these bacteria is studied. In this way, different signal patterns related to the metabolic response of microorganisms can be identified. By means of calibration curves after glucose uptake, the overall extracellular acidification of bacterial populations within the fermentation process can be evaluated.
Y1  - 2020
UR  - https://opus.bibliothek.fh-aachen.de/opus4/frontdoor/index/index/docId/9258
SN  - 0925-4005
VL  - 320
IS  - Art. 128232
PB  - Elsevier
CY  - Amsterdam
ER  -