TY  - JOUR
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Siqueira, José R. Jr.
A1  - Oliveira, Osvaldo N. Jr.
A1  - Moritz, Werner
A1  - Schöning, Michael Josef
T1  - Capacitive electrolyte-insulator-semiconductor structures functionalised with a polyelectrolyte/enzyme multilayer: New strategy for enhanced field-effect biosensing
T2  - Physica Status Solidi (A)
N2  - A novel strategy for enhanced field-effect biosensing using capacitive electrolyte–insulator–semiconductor (EIS) structures functionalised with pH-responsive weak polyelectrolyte/enzyme or dendrimer/enzyme multilayers is presented. The feasibility of the proposed approach is exemplarily demonstrated by realising a penicillin biosensor based on a capacitive p-Si–SiO2 EIS structure functionalised with a poly(allylamine hydrochloride) (PAH)/penicillinase and a poly(amidoamine) dendrimer/penicillinase multilayer. The developed sensors response to changes in both the local pH value near the gate surface and the charge of macromolecules induced via enzymatic reaction, resulting in a higher sensitivity. For comparison, an EIS penicillin biosensor with adsorptively immobilised penicillinase has been also studied. The highest penicillin sensitivity of 100 mV/dec has been observed for the EIS sensor functionalised with the PAH/penicillinase multilayer. The lower and upper detection limit was around 20 µM and 10 mM, respectively. In addition, an incorporation of enzymes in a multilayer prepared by layer-by-layer technique provides a larger amount of immobilised enzymes per sensor area, reduces enzyme leaching effects and thus, enhances the biosensor lifetime (the loss of penicillin sensitivity after 2 months was 10–12%).
Y1  - 2010
UR  - https://opus.bibliothek.fh-aachen.de/opus4/frontdoor/index/index/docId/3972
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
VL  - 207
IS  - 4
SP  - 884
EP  - 890
PB  - Wiley-VCH
CY  - Berlin
ER  -