@inproceedings{BayerHeschelerArtmannetal.2019, author = {Bayer, Robin and Hescheler, J{\"u}rgen and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Treating arterial hypertension in a cell culture well}, series = {3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH AachenW}, booktitle = {3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH AachenW}, editor = {Staat, Manfred and Erni, Daniel}, publisher = {Universit{\"a}t Duisburg-Essen}, address = {Duisburg}, organization = {MedTech Symposium}, isbn = {978-3-940402-22-6}, doi = {10.17185/duepublico/48750}, pages = {5 -- 6}, year = {2019}, abstract = {Hypertension describes the pathological increase of blood pressure, which is most commonly associated with the increase of vascular wall stiffness [1]. Referring to the "Deutsche Bluthochdruck Liga" this pathology shows a growing trend in our aging society. In order to find novel pharmacological and probably personalized treatments, we want to present a functional approach to study biomechanical properties of a human aortic vascular model. In this method review we will give an overview of recent studies which were carried out with the CellDrum technology [2] and underline the added value to already existing standard procedures known from the field of physiology. Herein described CellDrum technology is a system to measure functional mechanical properties of cell monolayers and thin tissue constructs in-vitro. Additionally, the CellDrum enables to elucidate the mechanical response of cells to pharmacological drugs, toxins and vasoactive agents. Due to its highly flexible polymer support, cells can also be mechanically stimulated by steady and cyclic biaxial stretching.}, language = {en} } @article{TemizArtmannKurulgandemirciFıratetal.2021, author = {Temiz Artmann, Ayseg{\"u}l and Kurulgan demirci, Eylem and F{\i}rat, Ipek Seda and Oflaz, Hakan and Artmann, Gerhard}, title = {Recombinant activated protein C (rhAPC) affects lipopolysaccharide-induced mechanical compliance changes and beat frequency of mESC-derived cardiomyocyte monolayers}, series = {SHOCK}, journal = {SHOCK}, publisher = {Wolters Kluwer}, address = {K{\"o}ln}, issn = {1540-0514}, doi = {10.1097/SHK.0000000000001845}, year = {2021}, language = {en} } @article{UysalCreutzFiratetal.2022, author = {Uysal, Karya and Creutz, Till and Firat, Ipek Seda and Artmann, Gerhard and Teusch, Nicole and Temiz Artmann, Ayseg{\"u}l}, title = {Bio-functionalized ultra-thin, large-area and waterproof silicone membranes for biomechanical cellular loading and compliance experiments}, series = {Polymers}, volume = {14}, journal = {Polymers}, number = {11}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, pages = {2213}, year = {2022}, abstract = {Biocompatibility, flexibility and durability make polydimethylsiloxane (PDMS) membranes top candidates in biomedical applications. CellDrum technology uses large area, <10 µm thin membranes as mechanical stress sensors of thin cell layers. For this to be successful, the properties (thickness, temperature, dust, wrinkles, etc.) must be precisely controlled. The following parameters of membrane fabrication by means of the Floating-on-Water (FoW) method were investigated: (1) PDMS volume, (2) ambient temperature, (3) membrane deflection and (4) membrane mechanical compliance. Significant differences were found between all PDMS volumes and thicknesses tested (p < 0.01). They also differed from the calculated values. At room temperatures between 22 and 26 °C, significant differences in average thickness values were found, as well as a continuous decrease in thicknesses within a 4 °C temperature elevation. No correlation was found between the membrane thickness groups (between 3-4 µm) in terms of deflection and compliance. We successfully present a fabrication method for thin bio-functionalized membranes in conjunction with a four-step quality management system. The results highlight the importance of tight regulation of production parameters through quality control. The use of membranes described here could also become the basis for material testing on thin, viscous layers such as polymers, dyes and adhesives, which goes far beyond biological applications.}, language = {en} } @article{UysalFiratCreutzetal.2022, author = {Uysal, Karya and Firat, Ipek Serat and Creutz, Till and Aydin, Inci Cansu and Artmann, Gerhard and Teusch, Nicole and Temiz Artmann, Ayseg{\"u}l}, title = {A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes}, series = {membranes}, volume = {2023}, journal = {membranes}, number = {13(1)}, publisher = {MDPI}, address = {Basel}, doi = {10.3390/membranes13010022}, pages = {Artikel 22}, year = {2022}, abstract = {Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5\% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here.}, language = {en} } @article{StaatKuehnHaugeretal.2004, author = {Staat, Manfred and K{\"u}hn, R. and Hauger, W. and Sponagel, Stefan}, title = {An Interpretation of Wolff's Law}, series = {Biomedizinische Technik. 49 (2004)}, journal = {Biomedizinische Technik. 49 (2004)}, isbn = {0932-4666}, pages = {1020 -- 1021}, year = {2004}, language = {de} } @article{StaatBaroudTopcuetal.2008, author = {Staat, Manfred and Baroud, G. and Topcu, M. and Sponagel, Stefan}, title = {Soft Materials in Technology and Biology - Characteristics, Properties, and Parameter Identification}, series = {Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)}, journal = {Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-540-75408-4}, pages = {253 -- 315}, year = {2008}, language = {en} } @article{StaatSponagelNguyen2010, author = {Staat, Manfred and Sponagel, Stefan and Nguyen, Nhu Huynh}, title = {Experiment and material model for soft tissue materials}, series = {Constitutive models for rubber VI : proceedings of the sixth European Conference on Constitutive Models for Rubber, Dresden, Germany, 7 - 10 September 2009 / eds. Gert Heinrich ...}, journal = {Constitutive models for rubber VI : proceedings of the sixth European Conference on Constitutive Models for Rubber, Dresden, Germany, 7 - 10 September 2009 / eds. Gert Heinrich ...}, publisher = {CRC Press}, address = {Boca Raton [u.a.]}, isbn = {978-0-415-56327-7}, pages = {465 -- 470}, year = {2010}, language = {en} } @article{KuehnHaugnerStaatetal.2004, author = {K{\"u}hn, Raoul-Roman and Haugner, Werner and Staat, Manfred and Sponagel, Stefan}, title = {A Two Phase Mixture Model based on Bone Observation}, year = {2004}, abstract = {An optimization method is developed to describe the mechanical behaviour of the human cancellous bone. The method is based on a mixture theory. A careful observation of the behaviour of the bone material leads to the hypothesis that the bone density is controlled by the principal stress trajectories (Wolff's law). The basic idea of the developed method is the coupling of a scalar value via an eigenvalue problem to the principal stress trajectories. On the one hand this theory will permit a prediction of the reaction of the biological bone structure after the implantation of a prosthesis, on the other hand it may be useful in engineering optimization problems. An analytical example shows its efficiency.}, subject = {Knochen}, language = {en} } @article{DoorschodtSchreinemachersBehbahanietal.2011, author = {Doorschodt, B. M. and Schreinemachers, M. C. J. M. and Behbahani, Mehdi and Florquin, S. and Weis, J. and Staat, Manfred and Tolba, R. H.}, title = {Hypothermic machine perfusion of kidney grafts: which pressure is preferred}, series = {Annals of Biomedical Engineering. 39 (2011), H. 3}, journal = {Annals of Biomedical Engineering. 39 (2011), H. 3}, publisher = {Springer}, address = {Berlin}, isbn = {1573-9686}, pages = {1051 -- 1059}, year = {2011}, language = {en} } @article{TemizArtmannKayatekinUysaletal.2005, author = {Temiz Artmann, Ayseg{\"u}l and Kayatekin, Muammer B. and Uysal, N. and Resmi, H.}, title = {Does antioxidant supplementation alter the effects of acute exercise on erythrocyte aggregation, deformability and endothelium adhesion in untrained rats? Kayatekin, Muammer B.; Uysal N.; Resmi, H.; Bediz, Seref C.; Temiz Artmann, A.; Gen{\c{c}}, S.; Tugyan, K.}, series = {International Journal for Vitamin and Nutrition Research. 75 (2005), H. 4}, journal = {International Journal for Vitamin and Nutrition Research. 75 (2005), H. 4}, isbn = {0300-9831}, pages = {243 -- 250}, year = {2005}, language = {en} }