@article{BogoyavlenskiyDigelBerezin1997, author = {Bogoyavlenskiy, A. P. and Digel, Ilya and Berezin, V. E.}, title = {Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates}, year = {1997}, abstract = {The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI)}, subject = {Enzyme-linked immunosorbent assay}, language = {en} } @article{ZhubanovaDigelNojimaetal.2007, author = {Zhubanova, Azhar A. and Digel, Ilya and Nojima, H. and Artmann, Gerhard}, title = {The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology}, year = {2007}, abstract = {The most of conventional methods of air purification use the power of a fan to draw in air and pass it through a filter. The problem of bacterial contamination of inner parts of such a type of air conditioners in some cases draws attention towards alternative air-cleaning systems. Some manufacturers offer to use the ozone's bactericidal and deodorizing effects, but the wide spreading of such systems is restricted by the fact that toxic effects of ozone in respect of human beings are well known. In 2000 Sharp Inc. introduced "Plasma Cluster Ions (PCI)" air purification technology, which uses plasma discharge to generate cluster ions (I 0-14 ). This technology has been developed for those customers that are conscious about health and hygiene. In our experiments, we focused on some principal aspects of plasma-generated ions application - time-dependency and irreversibility of bactericidal action, spatial and kinetic characteristics of emitted cluster particles, their chemical targets in the microbial cells.}, subject = {Clusterion}, language = {en} } @article{ZhubanovaAknazarovMansurovetal.2010, author = {Zhubanova, Azhar A. and Aknazarov, S. K. and Mansurov, Zulkhair and Digel, Ilya and Kozhalakova, A. A. and Akimbekov, Nuraly Shardarbekovich and O'Heras, Carlos and Tazhibayeva, S. and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials}, year = {2010}, abstract = {Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.}, subject = {Kohlenstofffaser}, language = {en} } @article{SeifarthSchehlLinderetal.2011, author = {Seifarth, Volker and Schehl, D. and Linder, Peter and Gossmann, Matthias and Digel, Ilya and Artmann, Gerhard and Porst, Dariusz and Preiß, C. and Kayser, Peter and Pack, O. and Temiz Artmann, Ayseg{\"u}l}, title = {Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds}, year = {2011}, abstract = {The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 \%. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days.}, subject = {Tissue Engineering}, language = {en} } @article{PreissLinderWendtetal.2011, author = {Preiß, C. and Linder, Peter and Wendt, K. and Krystek, M. and Digel, Ilya and Gossmann, Matthias and Temiz Artmann, Ayseg{\"u}l and Porst, Dariusz and Kayser, Peter and Bassam, Rasha and Artmann, Gerhard}, title = {Engineering technology for plant physiology and plant stress research}, year = {2011}, abstract = {Plant physiology and plant stress: Plant physiology will be much more important for human mankind because of yield and cultivation limits of crops determined by their resistance to stress. To assess and counteract various stress factors it is necessary to conduct plant research to gain information and results on plant physiology.}, subject = {Pflanzenphysiologie}, language = {en} } @article{BassamArtmannHescheleretal.2011, author = {Bassam, Rasha and Artmann, Gerhard and Hescheler, J{\"u}rgen and Graef, T. and Temiz Artmann, Ayseg{\"u}l and Porst, Dariusz and Linder, Peter and Kayser, Peter and Arinkin, Vladimir and Gossmann, Matthias and Digel, Ilya}, title = {Alterations in human hemoglobin structure related to red blood cell storage}, year = {2011}, abstract = {The importance of the availability of stored blood or blood cells, respectively, for urgent transfusion cannot be overestimated. Nowadays, blood storage becomes even more important since blood products are used for epidemiological studies, bio-technical research or banked for transfusion purposes. Thus blood samples must not only be processed, stored, and shipped to preserve their efficacy and safety, but also all parameters of storage must be recorded and reported for Quality Assurance. Therefore, blood banks and clinical research facilities are seeking more accurate, automated means for blood storage and blood processing.}, subject = {H{\"a}moglobin}, language = {en} } @article{GorissenStaatLaack2012, author = {Gorissen, P. and Staat, Manfred and Laack, Walter van}, title = {Experimentelle Kraftmessungen als Beitrag zur Wirksamkeitsbeurteilung von Schienbeinschonern im Fußballsport}, series = {OUP Zeitschrift f{\"u}r die orthop{\"a}dische und unfallchirurgische Praxis}, volume = {1}, journal = {OUP Zeitschrift f{\"u}r die orthop{\"a}dische und unfallchirurgische Praxis}, number = {1}, publisher = {Deutscher {\"A}rzte-Verlag}, address = {K{\"o}ln}, issn = {2193-5785}, doi = {10.3238/oup.2012.0010-0015}, pages = {10 -- 15}, year = {2012}, abstract = {Diese Studie besch{\"a}ftigte sich mit der D{\"a}mpfungswirkung von Schienbeinschonern, wie sie beim Fußball zum Einsatz kommen. Sie wurde mit Hilfe eines Pendelhammers durchgef{\"u}hrt, der verschiedene Aufschlagkr{\"a}fte auf die Schoner erm{\"o}glichte. Dabei wurde deutlich, dass Schienbeinschoner die beste Wirkung bei Maximalkr{\"a}ften unterhalb von 5kN erreichen k{\"o}nnen, dass bei gr{\"o}ßerer Belastung allerdings Verbesserungsbedarf besteht. Hierf{\"u}r konnte, u.a. durch den Einsatz neuer Materialien, ein guter Ansatzpunkt im „ad{\"a}quaten Zusammenspiel von Schale und Polsterung" der Schoner gefunden werden. Die Untersuchung hat weiterhin gezeigt, dass zumindest teilweise eine deutliche Verbesserung der D{\"a}mpfungswirkung der Schienbeinschoner in den letzten Jahren erreicht werden konnte.}, subject = {Fußball}, language = {de} } @inproceedings{SchoeningAbdelghani2012, author = {Sch{\"o}ning, Michael Josef and Abdelghani, Adnane}, title = {Nanoscale Science and Technology (NS\&T'12) : Proceedings Book Humboldt Kolleg <2012, Tunisia> ; Tunisia, 17-19 March, 2012 / ed. by Michael J. Sch{\"o}ning ; Adnane Abdelghani}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-3544}, year = {2012}, abstract = {Proceedings of the 2nd Humboldt Kolleg, Hammamet, Tunisia Organizer: Alexander von Humboldt Stiftung, Germany. pdf 184 p. Welcome Address Dear Participants, Welcome to the 2nd Humboldt Kolleg in "Nanoscale Science and Technology" (NS\&T'12) in Tunisia, sponsored by the "Alexander von Humboldt" foundation. The NS\&T'12 multidisciplinary scientific program includes seven "hot" topics dealing with "Nanoscale Science and Technology" covering basic and application-oriented research as well as industrial (market) aspects: - Molecular Biophyics, Spectroscopy Techniques, Imaging Microscopy - Nanomaterials Synthesis for Medicine and Bio-chemical Sensors - Nanostructures, Semiconductors, Photonics and Nanodevices - New Technologies in Market Industry - Environment, Electro-chemistry, Bio-polymers and Fuel Cells - Nanomaterials, Photovoltaic, Modelling, Quantum Physics - Microelectronics, Sensors Networks and Embedded Systems We are deeply indebted to all members of the Scientific Committee and General Chairs for joint Sessions and to all speakers and chairmen, who have dedicated invaluable time and efforts for the realization of this event. On behalf of the Organizing Committee, we are cordially inviting you to join the conference and hope that your stay will be fruitful, rewarding and enjoyable. Prof. Dr. Michael J. Sch{\"o}ning, Prof. Dr. Adnane Abdelghani}, subject = {Biosensor}, language = {en} } @article{LeversStaatLaack2011, author = {Levers, A. and Staat, Manfred and Laack, Walter van}, title = {Analyse der Langzeitwirkung der MBST® KernspinResonanzTherapie bei Gonarthrose}, year = {2011}, abstract = {Originalausgabe: Orthop{\"a}dische Praxis Jg. 47. 2011 H. 11; S. 536-543. Mit freundlicher Genehmigung des Verlags Zusammenfassung: Auf der Basis von Patientenabfragen mittels Fragebogen zum Schmerzempfinden und zur Einschr{\"a}nkung bei Aktivit{\"a}ten des allt{\"a}glichen Lebens wird die Langzeitwirkung der MBST® KernspinResonanz-Therapie bei Gonarthrose untersucht. An der Studie nahmen 39 Patienten teil, bei denen die Therapie bis zu vier Jahre zur{\"u}ckliegt. Neben einer Gesamtbetrachtung wird der Erfolg auch in Abh{\"a}ngigkeit von Alter, Geschlecht und sportlicher Aktivit{\"a}t analysiert. Insgesamt weist die Studie auf eine anhaltende Verbesserung des Gesundheitszustands mit zum Teil deutlicher Schmerzlinderung auch noch nach vier Jahren hin, jedoch mit einer leichten Schmerzzunahme gegen Ende des Untersuchungszeitraums von vier Jahren. Eine tendenziell positivere Wirkung bei Frauen, {\"a}lteren Menschen oder auch sportlich nicht-aktiven Patienten l{\"a}sst auf eine m{\"o}gliche Beeinflussung des Erfolgs der Therapie durch ({\"U}ber-)Belastung im Alltag schließen. Ein zus{\"a}tzlich positiver Effekt der Therapie auf die Knochendichte ist ebenfalls denkbar, dies bleibt jedoch offen.}, subject = {Kniegelenkarthrose}, language = {de} } @inproceedings{TranNovacekTolbaetal.2011, author = {Tran, Thanh Ngoc and Novacek, V. and Tolba, R. and Klinge, U. and Turquier, F. and Staat, Manfred}, title = {Experimental and Computational approach to study colorectal anastomosis. ISB2011, Proceedings of the XXIII Congress of the International Society of Biomechanics, Brussels, Belgium, July 3-7, 2011}, year = {2011}, abstract = {Summary: This paper presents a methodology to study and understand the mechanics of stapled anastomotic behaviors by combining empirical experimentation and finite element analysis. Performance of stapled anastomosis is studied in terms of leakage and numerical results which are compared to in vitro experiments performed on fresh porcine tissue. Results suggest that leaks occur between the tissue and staple legs penetrating through the tissue.}, subject = {Anastomose}, language = {en} }