@article{KarschuckKaulenPoghossianetal.2021, author = {Karschuck, Tobias and Kaulen, Corinna and Poghossian, Arshak and Wagner, Patrick H. and Sch{\"o}ning, Michael Josef}, title = {Gold nanoparticle-modified capacitive field-effect sensors: Studying the surface density of nanoparticles and coupling of charged polyelectrolyte macromolecules}, series = {Electrochemical Science Advances}, volume = {2}, journal = {Electrochemical Science Advances}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0938-5193}, doi = {10.1002/elsa.202100179}, pages = {10 Seiten}, year = {2021}, abstract = {The coupling of ligand-stabilized gold nanoparticles with field-effect devices offers new possibilities for label-free biosensing. In this work, we study the immobilization of aminooctanethiol-stabilized gold nanoparticles (AuAOTs) on the silicon dioxide surface of a capacitive field-effect sensor. The terminal amino group of the AuAOT is well suited for the functionalization with biomolecules. The attachment of the positively-charged AuAOTs on a capacitive field-effect sensor was detected by direct electrical readout using capacitance-voltage and constant capacitance measurements. With a higher particle density on the sensor surface, the measured signal change was correspondingly more pronounced. The results demonstrate the ability of capacitive field-effect sensors for the non-destructive quantitative validation of nanoparticle immobilization. In addition, the electrostatic binding of the polyanion polystyrene sulfonate to the AuAOT-modified sensor surface was studied as a model system for the label-free detection of charged macromolecules. Most likely, this approach can be transferred to the label-free detection of other charged molecules such as enzymes or antibodies.}, language = {en} } @article{KarschuckPoghossianSeretal.2024, author = {Karschuck, Tobias and Poghossian, Arshak and Ser, Joey and Tsokolakyan, Astghik and Achtsnicht, Stefan and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Capacitive model of enzyme-modified field-effect biosensors: Impact of enzyme coverage}, series = {Sensors and Actuators B: Chemical}, volume = {408}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005 (Print)}, doi = {10.1016/j.snb.2024.135530}, pages = {12 Seiten}, year = {2024}, abstract = {Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.}, language = {en} } @inproceedings{KasperSchiffelsKrafftetal.2016, author = {Kasper, Katharina and Schiffels, Johannes and Krafft, Simone and Kuperjans, Isabel and Elbers, Gereon and Selmer, Thorsten}, title = {Biogas Production on Demand Regulated by Butyric Acid Addition}, series = {IOP Conference Series: Earth and Environmental Science. Bd. 32}, volume = {32}, booktitle = {IOP Conference Series: Earth and Environmental Science. Bd. 32}, issn = {1755-1315}, doi = {10.1088/1755-1315/32/1/012009}, pages = {012009/1 -- 012009/4}, year = {2016}, language = {en} } @article{KassabHanPoghossianetal.2004, author = {Kassab, T. and Han, Y. and Poghossian, Arshak and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Detection of layerby-layer adsorbed polyelectrolytes by means of field-effect based capacitive EIS structures}, series = {Biomedizinische Technik. 49 (2004), H. 2}, journal = {Biomedizinische Technik. 49 (2004), H. 2}, isbn = {0932-4666}, pages = {1034 -- 1035}, year = {2004}, language = {en} } @article{KatzPoghossianSchoening2017, author = {Katz, Evgeny and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Enzyme-based logic gates and circuits - analytical applications and interfacing with electronics}, series = {Analytical and Bioanalytical Chemistry}, volume = {409}, journal = {Analytical and Bioanalytical Chemistry}, publisher = {Springer}, address = {Berlin}, issn = {1618-2650}, doi = {10.1007/s00216-016-0079-7}, pages = {81 -- 94}, year = {2017}, abstract = {The paper is an overview of enzyme-based logic gates and their short circuits, with specific examples of Boolean AND and OR gates, and concatenated logic gates composed of multi-step enzyme-biocatalyzed reactions. Noise formation in the biocatalytic reactions and its decrease by adding a "filter" system, converting convex to sigmoid response function, are discussed. Despite the fact that the enzyme-based logic gates are primarily considered as components of future biomolecular computing systems, their biosensing applications are promising for immediate practical use. Analytical use of the enzyme logic systems in biomedical and forensic applications is discussed and exemplified with the logic analysis of biomarkers of various injuries, e.g., liver injury, and with analysis of biomarkers characteristic of different ethnicity found in blood samples on a crime scene. Interfacing of enzyme logic systems with modified electrodes and semiconductor devices is discussed, giving particular attention to the interfaces functionalized with signal-responsive materials. Future perspectives in the design of the biomolecular logic systems and their applications are discussed in the conclusion.}, language = {en} } @article{KeusgenJuengerKnobbeetal.2003, author = {Keusgen, M. and J{\"u}nger, M. and Knobbe, D.-T. and Kloock, Joachim P. and Sch{\"o}ning, Michael Josef}, title = {Entwicklung eines potentiometrischen Cyanid-Biosensors auf der Basis von EIS-Strukturen}, series = {Sensoren f{\"u}r zuk{\"u}nftige Hochtechnologien und Neuentwicklungen f{\"u}r die Verfahrenstechnik / 6. Dresdner Sensor-Symposium, 8. - 10. Dezember 2003, Dresden. J{\"o}rg Peter Baselt; Gerald Gerlach (Hg.)}, journal = {Sensoren f{\"u}r zuk{\"u}nftige Hochtechnologien und Neuentwicklungen f{\"u}r die Verfahrenstechnik / 6. Dresdner Sensor-Symposium, 8. - 10. Dezember 2003, Dresden. J{\"o}rg Peter Baselt; Gerald Gerlach (Hg.)}, publisher = {w.e.b.-Univ.-Verl.}, address = {Dresden}, isbn = {3-935712-92-8}, pages = {235 -- 238}, year = {2003}, language = {de} } @article{KeusgenJuengerKrestetal.2003, author = {Keusgen, M. and J{\"u}nger, M. and Krest, I. and Sch{\"o}ning, Michael Josef}, title = {Development of a biosensor specific for cysteine sulfoxides}, series = {Biosensors \& Bioelectronics. 18 (2003), H. 5-6}, journal = {Biosensors \& Bioelectronics. 18 (2003), H. 5-6}, isbn = {0956-5663}, pages = {805 -- 812}, year = {2003}, language = {en} } @article{KeusgenJuengerSchoening2002, author = {Keusgen, M. and J{\"u}nger, M. and Sch{\"o}ning, Michael Josef}, title = {Biosensoric detection of the cysteine sulphoxide alliin}, series = {Book of abstracts / ed. by J. Saneistr.}, journal = {Book of abstracts / ed. by J. Saneistr.}, publisher = {Czech Technical University, Faculty of Electrical Engineering, Department of Measurement}, address = {Prague}, isbn = {80-01-02576-4}, pages = {1175 -- 1178}, year = {2002}, language = {en} } @article{KeusgenKloockKnobbeetal.2004, author = {Keusgen, M. and Kloock, Joachim P. and Knobbe, D.-T. and J{\"u}nger, M. and Krest, I. and Goldbach, M. and Klein, W. and Sch{\"o}ning, Michael Josef}, title = {Direct determination of cyanides by potentiometric biosensors}, series = {Sensors and Actuators B. 103 (2004), H. 1-2}, journal = {Sensors and Actuators B. 103 (2004), H. 1-2}, isbn = {0925-4005}, pages = {380 -- 385}, year = {2004}, language = {en} } @article{KeusgenSchoening2004, author = {Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Strategies for biosensoric detection of potential drugs in nature}, series = {Biomedizinische Technik. 49 (2004), H. 2}, journal = {Biomedizinische Technik. 49 (2004), H. 2}, isbn = {0932-4666}, pages = {1004 -- 1005}, year = {2004}, language = {en} }