@article{GeMeyerSchoeningetal.2000, author = {Ge, B. and Meyer, T. and Sch{\"o}ning, Michael Josef and Wollenberger, U. and Lisdat, F.,}, title = {Cytochrome c' from Chromatium vinosum on gold electrodes}, series = {Electrochemistry Communications. 2 (2000), H. 8}, journal = {Electrochemistry Communications. 2 (2000), H. 8}, isbn = {1388-2481}, pages = {557 -- 561}, year = {2000}, language = {en} } @article{PinkenburgSchiffelsSelmer2016, author = {Pinkenburg, Olaf and Schiffels, Johannes and Selmer, Thorsten}, title = {Das CoLibry-Konzept - ein Werkzeugkasten f{\"u}r die Synthetische Biologie: Bioproduktion}, series = {BIOspektrum}, volume = {22}, journal = {BIOspektrum}, number = {6}, publisher = {Springer}, address = {Berlin}, doi = {10.1007/s12268-016-0734-8}, pages = {593 -- 595}, year = {2016}, abstract = {Regardless of size or destination, synthetic biology starts with com-parably small information units, which need to be combined and properly arranged in order to achieve a certain goal. This may be the de novo synthesis of individual genes from oligonucleotides, a shuffling of protein domains in order to create novel biocatalysts, the assembly of multiple enzyme encoding genes in metabolic pathway design, or strain development at the production stage. The CoLibry concept has been designed in order to close the gap between recombinant production of individual genes and genome editing.}, language = {de} } @article{HeiduschkaRomannEckenetal.2001, author = {Heiduschka, P. and Romann, I. and Ecken, H. and Sch{\"o}ning, Michael Josef and Schuhmann, W. and Thanos, S.}, title = {Defined adhesion and growth of neurones on artificial structured substrates}, series = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, journal = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, publisher = {Elsevier [u.a.]}, address = {Amsterdam [u.a.]}, isbn = {0-08-044014-2}, pages = {299 -- 307}, year = {2001}, language = {en} } @article{SchusserMenzelBaeckeretal.2013, author = {Schusser, Sebastian and Menzel, S. and B{\"a}cker, Matthias and Leinhos, Marcel and Poghossian, Arshak and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Degradation of thin poly(lactic acid) films: characterization by capacitance-voltage, atomic force microscopy, scanning electron microscopy and contact-angle measurements}, series = {Electrochimica Acta}, volume = {Vol. 113}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-3859 (E-Journal); 0013-4686 (Print)}, pages = {779 -- 784}, year = {2013}, language = {en} } @article{BaeckerSchusserPoghossianetal.2013, author = {B{\"a}cker, Matthias and Schusser, Sebastian and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Degradationsverhalten bioabbaubarer Polymere : siliziumbasierte Sensorik zur {\"U}berwachung}, series = {GIT Labor-Fachzeitschrift}, journal = {GIT Labor-Fachzeitschrift}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0016-3538}, pages = {32 -- 33}, year = {2013}, language = {de} } @article{SchoeningSchuetz2009, author = {Sch{\"o}ning, Michael Josef and Sch{\"u}tz, S.}, title = {Der K{\"a}fer/Chip-Sensor als bioelektronischer "Schn{\"u}ffler"}, series = {Bioanalytik f{\"u}r Einsteiger : [Diabetes, Drogen und DNA] / Reinhard Renneberg. Darja S{\"u}ßbier (Ill.). [Red.: Andreas Held ...]}, journal = {Bioanalytik f{\"u}r Einsteiger : [Diabetes, Drogen und DNA] / Reinhard Renneberg. Darja S{\"u}ßbier (Ill.). [Red.: Andreas Held ...]}, publisher = {Spektrum, Akad. Verl.}, address = {Heidelberg}, isbn = {978-3-8274-1831-9}, pages = {204 -- 205}, year = {2009}, language = {de} } @article{AlmajhdiSengerAmeretal.2014, author = {Almajhdi, Fahad N. and Senger, Tilo and Amer, Haitham M. and Gissmann, Lutz and {\"O}hlschl{\"a}ger, Peter}, title = {Design of a highly effective therapeutic HPV16 E6/E7-specific DNA vaccine: optimization by different ways of sequence rearrangements (Shuffling)}, series = {PLOS one}, volume = {11}, journal = {PLOS one}, number = {9}, publisher = {PLOS}, address = {San Francisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0113461}, pages = {e113461}, year = {2014}, abstract = {Persistent infection with the high-risk Human Papillomavirus type 16 (HPV 16) is the causative event for the development of cervical cancer and other malignant tumors of the anogenital tract and of the head and neck. Despite many attempts to develop therapeutic vaccines no candidate has entered late clinical trials. An interesting approach is a DNA based vaccine encompassing the nucleotide sequence of the E6 and E7 viral oncoproteins. Because both proteins are consistently expressed in HPV infected cells they represent excellent targets for immune therapy. Here we report the development of 8 DNA vaccine candidates consisting of differently rearranged HPV-16 E6 and E7 sequences within one molecule providing all naturally occurring epitopes but supposedly lacking transforming activity. The HPV sequences were fused to the J-domain and the SV40 enhancer in order to increase immune responses. We demonstrate that one out of the 8 vaccine candidates induces very strong cellular E6- and E7- specific cellular immune responses in mice and, as shown in regression experiments, efficiently controls growth of HPV 16 positive syngeneic tumors. This data demonstrates the potential of this vaccine candidate to control persistent HPV 16 infection that may lead to malignant disease. It also suggests that different sequence rearrangements influence the immunogenecity by an as yet unknown mechanism.}, language = {en} } @article{PoghossianSchoening2004, author = {Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Detecting Both Physical and (Bio-)Chemical Parameters by Means of ISFET Devices}, series = {Electroanalysis. 16 (2004), H. 22}, journal = {Electroanalysis. 16 (2004), H. 22}, isbn = {1040-0397}, pages = {1863 -- 1872}, year = {2004}, language = {en} } @article{WeldenSeverinsPoghossianetal.2022, author = {Welden, Melanie and Severins, Robin and Poghossian, Arshak and Wege, Christina and Bongaerts, Johannes and Siegert, Petra and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor}, series = {Chemosensors}, volume = {10}, journal = {Chemosensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors10060218}, pages = {Artikel 218}, year = {2022}, abstract = {Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte-insulator-semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin-streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage-current, capacitance-voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.}, language = {en} } @inproceedings{MolinnusHardtKaeveretal.2017, author = {Molinnus, Denise and Hardt, Gabriel and K{\"a}ver, Larissa and Willenberg, Holger S. and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology}, series = {MDPI Proceedings}, booktitle = {MDPI Proceedings}, doi = {10.3390/proceedings1040506}, pages = {4 Seiten}, year = {2017}, language = {en} }