@article{KochWunderlichSeibleretal.2008, author = {Koch, Linda and Wunderlich, F. Thomas and Seibler, Jost and K{\"o}nner, A. Christine and Hampel, Brigitte and Irlenbusch, Sigrid and Brabant, Georg and Kahn, C. Ronald and Schwenk, Frieder and Br{\"u}ning, Jens C.}, title = {Central insulin action regulates peripheral glucose and fat metabolism in mice}, series = {The Journal of Clinical Investigation (JCI)}, volume = {118}, journal = {The Journal of Clinical Investigation (JCI)}, number = {6}, issn = {1558-8238}, doi = {10.1172/JCI31073}, pages = {2132 -- 2147}, year = {2008}, language = {en} } @article{HandtkeVollandMethlingetal.2014, author = {Handtke, Stefan and Volland, Sonja and Methling, Karen and Albrecht, Dirk and Becher, D{\"o}rte and Nehls, Jenny and Bongaerts, Johannes and Maurer, Karl-Heinz and Lalk, Michael and Liesegang, Heiko and Voigt, Birgit and Daniel, Rolf and Hecker, Michael}, title = {Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach}, series = {Journal of Biotechnology}, journal = {Journal of Biotechnology}, number = {192(A)}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2014.08.028}, pages = {204 -- 214}, year = {2014}, abstract = {Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43\% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC-MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, Doris and Karl, Wolfgang and Birner-Gruenberger, Ruth and Gruber, Karl and Eiteljoerg, Inge and Remler, Peter and Wieland, Susanne and Siegert, Petra and Maurer, Karl-Heinz and Schwab, Helmut}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{AboulnagaPinkenburgSchiffelsetal.2013, author = {Aboulnaga, El-Hussiny and Pinkenburg, Olaf and Schiffels, Johannes and El-Refai, Ahmed and Buckel, Wolfgang and Selmer, Thorsten}, title = {Butyrate production in Escherichia coli: Exploitation of an oxygen tolerant bifurcating butyryl-CoA dehydrogenase/electron transferring flavoprotein complex from Clostridium difficile}, series = {Journal of bacteriology. June 14, 2013}, journal = {Journal of bacteriology. June 14, 2013}, issn = {1098-5530 (E-Journal) ; 0021-9193 (Print)}, pages = {Epub ahead of print}, year = {2013}, language = {de} } @article{Lassonczyk1991, author = {Lassonczyk, Beate}, title = {Bodenentwicklung auf Terrassenschottern im ariden Bereich von NO-Somalia}, series = {Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 66 (1991), H. 2}, journal = {Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 66 (1991), H. 2}, isbn = {0343-107x}, pages = {1153 -- 1156}, year = {1991}, language = {de} } @article{AlKaidyDuweHusteretal.2015, author = {Al-Kaidy, Huschyar and Duwe, Anna and Huster, Manuel and Muffler, Kai and Schlegel, Christin and Tim, Sieker and Stadtm{\"u}ller, Ralf and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Biotechnology and bioprocess engineering - from the first ullmann's article to recent trends}, series = {ChemBioEng Reviews}, volume = {2}, journal = {ChemBioEng Reviews}, number = {3}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/cben.201500008}, pages = {175 -- 184}, year = {2015}, abstract = {For several thousand years, biotechnology and its associated technical processes have had a great impact on the development of mankind. Based on empirical methods, in particular for the production of foodstuffs and daily commodities, these disciplines have become one of the most innovative future issues. Due to the increasing detailed understanding of cellular processes, production strains can now be optimized. In combination with modern bioprocesses, a variety of bulk and fine chemicals as well as pharmaceuticals can be produced efficiently. In this article, some of the current trends in biotechnology are discussed.}, language = {en} } @article{BiselliHilbertJelineketal.2000, author = {Biselli, Manfred and Hilbert, Ulrike and Jelinek, Nanni and Schmidt, Sebastian}, title = {Biotechnologische Aspekte der Kultivierung humaner T-Lymphozyten / Hilbert, Ulrike ; Jelinek, Nanni ; Schmidt, Sebastian ; Biselli, Manfred}, series = {Chemie Ingenieur Technik. 72 (2000), H. 7}, journal = {Chemie Ingenieur Technik. 72 (2000), H. 7}, isbn = {1522-2640}, pages = {746 -- 750}, year = {2000}, language = {de} } @article{AlKaidyDuweHusteretal.2014, author = {Al-Kaidy, Huschyar and Duwe, Anna and Huster, Manuel and Muffler, Kai and Schlegel, Christin and Sieker, Tim and Stadtm{\"u}ller, Ralf and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Biotechnologie und Bioverfahrenstechnik - Vom ersten Ullmanns Artikel bis hin zu aktuellen Forschungsthemen}, series = {Chemie Ingenieur Technik}, volume = {86}, journal = {Chemie Ingenieur Technik}, number = {12}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0009-286X}, doi = {10.1002/cite.201400083}, pages = {2215 -- 2225}, year = {2014}, abstract = {Biotechnologie und die mit ihr verbundenen technischen Prozesse pr{\"a}gen seit Jahrtausenden die Entwicklung der Menschheit. Ausgehend von empirischen Verfahren, insbesondere zur Herstellung von Lebensmitteln und t{\"a}glichen Gebrauchsg{\"u}tern, haben sich diese Disziplinen zu einem der innovativsten Zukunftsfelder entwickelt. Durch das immer detailliertere Verst{\"a}ndnis zellul{\"a}rer Vorg{\"a}nge k{\"o}nnen mittlerweile Produktionsst{\"a}mme gezielt optimiert werden. Im Zusammenspiel mit moderner Prozesstechnik k{\"o}nnen so eine Vielzahl von Bulk- und Feinchemikalien sowie Pharmazeutika effizient hergestellt werden. In diesem Artikel werden exemplarisch einige der aktuellen Trends vorgestellt.}, language = {de} } @article{BiselliJelinekSchmidtetal.2001, author = {Biselli, Manfred and Jelinek, N. and Schmidt, S. and Noll, T.}, title = {Bioreaktoren zur Kultivierung blutbildender Zellen / Jelinek, N. ; Schmidt, S. ; Noll, T. ; Biselli, M.}, series = {Bioforum. 24 (2001), H. 6}, journal = {Bioforum. 24 (2001), H. 6}, isbn = {0940-0079}, pages = {418 -- 419}, year = {2001}, language = {de} } @article{BiselliHilbertJelineketal.2001, author = {Biselli, Manfred and Hilbert, U. and Jelinek, N. and Schmidt, S.}, title = {Bioprocess development for the cultivation of human T-lymphocytes / Hilbert, U. ; Jelinek, N. Schmidt, S. ; Biselli, M.}, series = {Engineering in Life Sciences. 1 (2001)}, journal = {Engineering in Life Sciences. 1 (2001)}, isbn = {1618-0240}, pages = {20 -- 23}, year = {2001}, language = {en} }