@inproceedings{GoldbachAment2010, author = {Goldbach, Daniel and Ament, Christoph}, title = {Stabilized reversing with vehicle trailer combinations}, series = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau}, booktitle = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau}, editor = {Scharff, Peter}, publisher = {Verl. ISLE}, address = {Ilmenau}, organization = {Technische Universit{\"a}t / Fakult{\"a}t f{\"u}r Informatik und Automatisierung}, isbn = {978-3-938843-53-6}, pages = {352 -- 357}, year = {2010}, language = {en} } @misc{Alt2010, author = {Alt, Helmut}, title = {Vom Studium der Kerntechnik zu Energie zwischen Wunsch und Wirklichkeit : [Vortrag : Verleihung der KTG-Ehrenmitgliedschaft, Berlin, November 2010]}, pages = {1 -- 15}, year = {2010}, language = {de} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @misc{SiegertMussmannO'Connelletal.2010, author = {Siegert, Petra and Mussmann, Nina and O'Connell, Timothy and Maurer, Karl-Heinz}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt / WIPO}, address = {M{\"u}nchen / Genf}, pages = {1 -- 30}, year = {2010}, language = {de} } @misc{SiegertBaumstarkKluinetal.2010, author = {Siegert, Petra and Baumstark, Rebecca and Kluin, Cornelia and O'Connell, Timothy and Maurer, Karl-Heinz and Hellmuth, Hendrik}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {1 -- 30}, year = {2010}, language = {de} } @misc{SiegertSpitzMaurer2010, author = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patentamt / WIPO}, address = {M{\"u}nchen / Genf}, pages = {1 -- 31}, year = {2010}, language = {de} } @misc{SiegertSpitzMaurer2010, author = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz}, title = {Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift]}, publisher = {Deutsches Patentamt / Europ{\"a}isches Patentamt / WIPO}, address = {M{\"u}nchen / Den Hague / Genf}, pages = {1 -- 20}, year = {2010}, language = {de} } @incollection{BudaSchuermannWollert2010, author = {Buda, Aurel and Sch{\"u}rmann, Volker and Wollert, J{\"o}rg}, title = {Wireless technologies in factory automation}, series = {Factory automation / ed. by Javier Silvestre-Blanes}, booktitle = {Factory automation / ed. by Javier Silvestre-Blanes}, publisher = {Intech}, address = {London}, isbn = {978-953-7619-42-8}, pages = {29 -- 50}, year = {2010}, language = {en} } @article{WollertLeidinger2010, author = {Wollert, J{\"o}rg and Leidinger, Rafael}, title = {Automation trifft Automotive : SPS in der Fahrzeugtechnik}, series = {Elektronik automotive : Magazin f{\"u}r Entwicklungen in der Kfz-Elektronik und Telematik}, journal = {Elektronik automotive : Magazin f{\"u}r Entwicklungen in der Kfz-Elektronik und Telematik}, number = {4/5}, publisher = {WEKA Fachmedien}, address = {Haar}, issn = {1614-0125}, pages = {44 -- 48}, year = {2010}, abstract = {Eine SPS im Kraftfahrzeug? Das geht ja gar nicht - da gibt es doch all die Hersteller f{\"u}r Kfz-Steuerger{\"a}te, die konfigurierbare Hardware anbieten. Im Prinzip ja, aber leider sind gerade die Sensoren oder Aktoren nicht ansteuerbar, die der Entwickler genau f{\"u}r sein aktuelles Projekt ben{\"o}tigt. Dieser Beitrag gibt einen kleinen Einblick in neue M{\"o}glichkeiten des Rapid Prototyping f{\"u}r mechatronische Systeme auf der Basis von Speicherprogrammierbaren Steuerungen (SPS).}, language = {de} } @inproceedings{SchuermannKellerWollert2010, author = {Sch{\"u}rmann, Volker and Keller, A. and Wollert, J{\"o}rg}, title = {Geb{\"a}udeautomation mit Webtechnologien : ein Erfahrungsbericht}, series = {Automation 2010 : der 11. Branchentreff der Mess- und Automatisierungstechnik ; Kongress Baden-Baden, 15. und 16. Juni 2010. (VDI-Berichte ; 2092)}, booktitle = {Automation 2010 : der 11. Branchentreff der Mess- und Automatisierungstechnik ; Kongress Baden-Baden, 15. und 16. Juni 2010. (VDI-Berichte ; 2092)}, publisher = {VDI-Verl.}, address = {D{\"u}sseldorf}, organization = {VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, isbn = {978-3-18-092092-4}, pages = {321 -- 324}, year = {2010}, language = {de} }