@article{HeuermannStolleSchiek1995, author = {Heuermann, Holger and Stolle, Reinhard and Schiek, Burkhard}, title = {Novel Algorithms for FMCW Range Finding with Microwaves. Stolle, R.; Heuermann, H.; Schiek, B.}, pages = {129 -- 132}, year = {1995}, language = {en} } @article{HaegerProbstJaegeretal.2023, author = {Haeger, Gerrit and Probst, Johanna and Jaeger, Karl-Erich and Bongaerts, Johannes and Siegert, Petra}, title = {Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans}, series = {FEBS Open Bio}, volume = {13}, journal = {FEBS Open Bio}, number = {12}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {2211-5463}, doi = {10.1002/2211-5463.13723}, pages = {2224 -- 2238}, year = {2023}, abstract = {Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.}, language = {en} } @article{RingersBialonskiEgeetal.2023, author = {Ringers, Christa and Bialonski, Stephan and Ege, Mert and Solovev, Anton and Hansen, Jan Niklas and Jeong, Inyoung and Friedrich, Benjamin M. and Jurisch-Yaksi, Nathalie}, title = {Novel analytical tools reveal that local synchronization of cilia coincides with tissue-scale metachronal waves in zebrafish multiciliated epithelia}, series = {eLife}, volume = {12}, journal = {eLife}, publisher = {eLife Sciences Publications}, issn = {2050-084X}, doi = {10.7554/eLife.77701}, pages = {27 Seiten}, year = {2023}, abstract = {Motile cilia are hair-like cell extensions that beat periodically to generate fluid flow along various epithelial tissues within the body. In dense multiciliated carpets, cilia were shown to exhibit a remarkable coordination of their beat in the form of traveling metachronal waves, a phenomenon which supposedly enhances fluid transport. Yet, how cilia coordinate their regular beat in multiciliated epithelia to move fluids remains insufficiently understood, particularly due to lack of rigorous quantification. We combine experiments, novel analysis tools, and theory to address this knowledge gap. To investigate collective dynamics of cilia, we studied zebrafish multiciliated epithelia in the nose and the brain. We focused mainly on the zebrafish nose, due to its conserved properties with other ciliated tissues and its superior accessibility for non-invasive imaging. We revealed that cilia are synchronized only locally and that the size of local synchronization domains increases with the viscosity of the surrounding medium. Even though synchronization is local only, we observed global patterns of traveling metachronal waves across the zebrafish multiciliated epithelium. Intriguingly, these global wave direction patterns are conserved across individual fish, but different for left and right noses, unveiling a chiral asymmetry of metachronal coordination. To understand the implications of synchronization for fluid pumping, we used a computational model of a regular array of cilia. We found that local metachronal synchronization prevents steric collisions, i.e., cilia colliding with each other, and improves fluid pumping in dense cilia carpets, but hardly affects the direction of fluid flow. In conclusion, we show that local synchronization together with tissue-scale cilia alignment coincide and generate metachronal wave patterns in multiciliated epithelia, which enhance their physiological function of fluid pumping.}, language = {en} } @article{MonakhovaDiehl2021, author = {Monakhova, Yulia and Diehl, Bernd W.K.}, title = {Novel approach of qNMR workflow by standardization using 2H integral: Application to any intrinsic calibration standard}, series = {Talanta}, volume = {222}, journal = {Talanta}, number = {Article number: 121504}, publisher = {Elsevier}, isbn = {0039-9140}, doi = {10.1016/j.talanta.2020.121504}, year = {2021}, abstract = {Quantitative nuclear magnetic resonance (qNMR) is routinely performed by the internal or external standardization. The manuscript describes a simple alternative to these common workflows by using NMR signal of another active nuclei of calibration compound. For example, for any arbitrary compound quantification by NMR can be based on the use of an indirect concentration referencing that relies on a solvent having both 1H and 2H signals. To perform high-quality quantification, the deuteration level of the utilized deuterated solvent has to be estimated. In this contribution the new method was applied to the determination of deuteration levels in different deuterated solvents (MeOD, ACN, CDCl3, acetone, benzene, DMSO-d6). Isopropanol-d6, which contains a defined number of deuterons and protons, was used for standardization. Validation characteristics (precision, accuracy, robustness) were calculated and the results showed that the method can be used in routine practice. Uncertainty budget was also evaluated. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and can be applied in different application areas (purity determination, forensics, pharmaceutical analysis, etc.).}, language = {en} } @article{Schoening2002, author = {Sch{\"o}ning, Michael Josef}, title = {Novel approaches to design siliconbased field-effect sensors}, series = {Electrochemical Microsystem Technologies, New Trends in Electrochemistry Vol. 2}, journal = {Electrochemical Microsystem Technologies, New Trends in Electrochemistry Vol. 2}, publisher = {Taylor \& Francis}, address = {London New York}, pages = {384 -- 408}, year = {2002}, language = {en} } @article{HeuermannSadeghfam2004, author = {Heuermann, Holger and Sadeghfam, Arash}, title = {Novel balanced inductor for compact differential systems / Sadeghfam, Arash; Heuermann, Holger}, series = {Conference proceedings : Tuesday 12th, Wednesday 13th and Thursday 14th October, [RAI International Exhibition and Congress Centre, Amsterdam ; part of European Microwave Week 2004] / EuMA, European Microwave Association}, journal = {Conference proceedings : Tuesday 12th, Wednesday 13th and Thursday 14th October, [RAI International Exhibition and Congress Centre, Amsterdam ; part of European Microwave Week 2004] / EuMA, European Microwave Association}, publisher = {Horizon House Publ.}, address = {London}, isbn = {1-580-53992-0}, pages = {709 -- 712}, year = {2004}, language = {en} } @article{BiselliJelinekSchmidtetal.2002, author = {Biselli, Manfred and Jelinek, N. and Schmidt, S. and Hilbert, U.}, title = {Novel bioreactors for the ex vivo cultivation of hematopoietic cells / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C.}, series = {Engineering in Life Sciences. 2 (2002), H. 1}, journal = {Engineering in Life Sciences. 2 (2002), H. 1}, isbn = {1618-0240}, pages = {15 -- 18}, year = {2002}, language = {en} } @article{WagnerMiyamotoSchoeningetal.2010, author = {Wagner, Torsten and Miyamoto, Ko-ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Novel combination of digital light processing (DLP) and light-addressable potentiometric sensors (LAPS) for flexible chemical imaging}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {520 -- 523}, year = {2010}, language = {en} } @article{HeuermannHillebrandSadeghfam2008, author = {Heuermann, Holger and Hillebrand, J{\"u}rgen and Sadeghfam, Arash}, title = {Novel Concept of a Digital SigmaDelta-PLL-Phase-Shift-Modulator / Hillebrand, J{\"u}rgen ; Sadeghfam, Arash ; Heuermann, Holger}, isbn = {978-3-8007-3086-5}, pages = {123 -- 126}, year = {2008}, language = {en} } @article{PoghossianBerndsenLuethetal.2001, author = {Poghossian, Arshak and Berndsen, Lars and L{\"u}th, Hans and Sch{\"o}ning, Michael Josef}, title = {Novel concepts for flow-rate and flow-direction determination by means of pH-sensitive ISFETs}, series = {Proceedings of SPIE. 4560 (2001)}, journal = {Proceedings of SPIE. 4560 (2001)}, pages = {19 -- 27}, year = {2001}, language = {en} }