@article{FalkenbergRahbaFischeretal.2022, author = {Falkenberg, Fabian and Rahba, Jade and Fischer, David and Bott, Michael and Bongaerts, Johannes and Siegert, Petra}, title = {Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T}, series = {FEBS Open Bio}, volume = {12}, journal = {FEBS Open Bio}, number = {10}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {2211-5463}, doi = {10.1002/2211-5463.13457}, pages = {1729 -- 1746}, year = {2022}, abstract = {Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present study aimed at the characterization of a high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T. The protease gene was cloned and expressed in Bacillus subtilis DB104. The recombinant protease SPAO with 269 amino acids belongs to the subfamily of high-alkaline subtilisins. The biochemical characteristics of purified SPAO were analyzed in comparison with subtilisin Carlsberg, Savinase, and BPN'. SPAO, a monomer with a molecular mass of 27.1 kDa, was active over a wide range of pH 6.0-12.0 and temperature 20-80 °C, optimally at pH 9.0-9.5 and 55 °C. The protease is highly oxidatively stable to hydrogen peroxide and retained 58\% of residual activity when incubated at 10 °C with 5\% (v/v) H2O2 for 1 h while stimulated at 1\% (v/v) H2O2. Furthermore, SPAO was very stable and active at NaCl concentrations up to 5.0 m. This study demonstrates the potential of SPAO for biotechnological applications in the future.}, language = {en} } @article{FalkenbergKohnBottetal.2023, author = {Falkenberg, Fabian and Kohn, Sophie and Bott, Michael and Bongaerts, Johannes and Siegert, Petra}, title = {Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ}, series = {FEBS Open Bio}, volume = {13}, journal = {FEBS Open Bio}, number = {11}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {2211-5463}, doi = {10.1002/2211-5463.13701}, pages = {2035 -- 2046}, year = {2023}, abstract = {Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5\% (w/v) SDS and 5\% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.}, language = {en} } @article{ThomessenThomaBraun2023, author = {Thomessen, Karolin and Thoma, Andreas and Braun, Carsten}, title = {Bio-inspired altitude changing extension to the 3DVFH* local obstacle avoidance algorithm}, series = {CEAS Aeronautical Journal}, journal = {CEAS Aeronautical Journal}, publisher = {Springer}, address = {Wien}, issn = {1869-5590 (Online)}, doi = {10.1007/s13272-023-00691-w}, pages = {11 Seiten}, year = {2023}, abstract = {Obstacle avoidance is critical for unmanned aerial vehicles (UAVs) operating autonomously. Obstacle avoidance algorithms either rely on global environment data or local sensor data. Local path planners react to unforeseen objects and plan purely on local sensor information. Similarly, animals need to find feasible paths based on local information about their surroundings. Therefore, their behavior is a valuable source of inspiration for path planning. Bumblebees tend to fly vertically over far-away obstacles and horizontally around close ones, implying two zones for different flight strategies depending on the distance to obstacles. This work enhances the local path planner 3DVFH* with this bio-inspired strategy. The algorithm alters the goal-driven function of the 3DVFH* to climb-preferring if obstacles are far away. Prior experiments with bumblebees led to two definitions of flight zone limits depending on the distance to obstacles, leading to two algorithm variants. Both variants reduce the probability of not reaching the goal of a 3DVFH* implementation in Matlab/Simulink. The best variant, 3DVFH*b-b, reduces this probability from 70.7 to 18.6\% in city-like worlds using a strong vertical evasion strategy. Energy consumption is higher, and flight paths are longer compared to the algorithm version with pronounced horizontal evasion tendency. A parameter study analyzes the effect of different weighting factors in the cost function. The best parameter combination shows a failure probability of 6.9\% in city-like worlds and reduces energy consumption by 28\%. Our findings demonstrate the potential of bio-inspired approaches for improving the performance of local path planning algorithms for UAV.}, language = {en} } @inproceedings{Uibel2009, author = {Uibel, Thomas}, title = {Beurteilung der Spaltgefahr beim Eindrehen von selbstbohrenden Holzschrauben}, series = {Forum Holz/Bau Garmisch 09 : Internationales Holzbauforum (IHF) ; Congress Centrum, Garmisch-Partenkirchen, 2. - 4. Dezember 2009 ; aus der Praxis - f{\"u}r die Praxis, Wirtschaft, Markt, {\"O}kologie, Energieeffizienz, Architektur, Tragwerke, Realisierungen}, booktitle = {Forum Holz/Bau Garmisch 09 : Internationales Holzbauforum (IHF) ; Congress Centrum, Garmisch-Partenkirchen, 2. - 4. Dezember 2009 ; aus der Praxis - f{\"u}r die Praxis, Wirtschaft, Markt, {\"O}kologie, Energieeffizienz, Architektur, Tragwerke, Realisierungen}, publisher = {Fraunhofer IRB}, address = {Stuttgart}, isbn = {978-3-8167-8187-5}, year = {2009}, language = {de} } @article{WingensMayPfaff2019, author = {Wingens, Niklas and May, J{\"o}rg and Pfaff, Raphael}, title = {Betrieblich-technische Innovationsans{\"a}tze zur Bremsprobe nach einer Prozess{\"a}nderung}, series = {Eisenbahntechnische Rundschau : ETR ; Zeitschrift f{\"u}r die gesamte Eisenbahntechnik}, journal = {Eisenbahntechnische Rundschau : ETR ; Zeitschrift f{\"u}r die gesamte Eisenbahntechnik}, number = {5}, publisher = {DVV Media Group}, address = {Hamburg}, issn = {0013-2845}, pages = {5}, year = {2019}, language = {de} } @article{PfaffGidaszewskiSchmidt2020, author = {Pfaff, Raphael and Gidaszewski, Lars and Schmidt, Bernd}, title = {Ber{\"u}cksichtigung von No Fault Found im Diagnose- und Instandhaltungssystem von Schienenfahrzeugen}, series = {ETR - Eisenbahntechnische Rundschau}, journal = {ETR - Eisenbahntechnische Rundschau}, number = {5}, publisher = {DVV Media Group}, address = {Hamburg}, issn = {0013-2845}, pages = {56 -- 59}, year = {2020}, abstract = {Intermittierende und nicht reproduzierbare Fehler, auch als No Fault Found bezeichnet, treten in praktisch allen Bereichen auf und sorgen f{\"u}r hohe Kosten. Diese sind h{\"a}ufig auf unpr{\"a}zise Fehlerbeschreibungen zur{\"u}ckzuf{\"u}hren. Im vorliegenden Beitrag werden Anpassungen der Vorgehensweise bei der Entwicklung und Anpassungen des Diagnosesystems vorgeschlagen.}, language = {de} } @article{BlassUibel2009, author = {Blaß, Hans Joachim and Uibel, Thomas}, title = {Bemessungsvorschl{\"a}ge f{\"u}r Verbindungsmittel in Brettsperrholz}, series = {Bauen mit Holz}, volume = {111}, journal = {Bauen mit Holz}, number = {2}, publisher = {Rudolf M{\"u}ller}, address = {K{\"o}ln}, issn = {0005-6545}, pages = {46 -- 53}, year = {2009}, language = {de} } @article{Uibel2007, author = {Uibel, Thomas}, title = {Bemessung stiftf{\"o}rmiger Verbindungsmittel in Brettsperrholz - Anwendung aktueller Forschungsergebnisse}, series = {Praktische Anwendung von Massivholzplatten : 39. Fortbildungskurs SAH Schweizerische Arbeitsgemeinschaft f{\"u}r Holzforschung ; 7./8. November 2007 in Weinfelden}, journal = {Praktische Anwendung von Massivholzplatten : 39. Fortbildungskurs SAH Schweizerische Arbeitsgemeinschaft f{\"u}r Holzforschung ; 7./8. November 2007 in Weinfelden}, publisher = {SAH}, address = {D{\"u}bendorf}, isbn = {3-906703-19-3}, pages = {183 -- 192}, year = {2007}, language = {de} } @misc{OPUS4-10900, title = {Beitragsordnung des Studierendenwerks Aachen (21. {\"A}nderung) : vom 3. Juli 2019}, organization = {FH Aachen}, pages = {2 Seiten}, year = {2019}, language = {de} } @misc{OPUS4-10943, title = {Beitragsordnung der Studierendenschaft der Fachhochschule Aachen : vom 11. Dezember 2018 - FH-Mitteilung Nr. 168/2018 in der Fassung der Bekanntmachung der {\"A}nderungsordnung vom 7. November 2019 - FH-Mitteilung Nr. 115/2019 (Nichtamtliche lesbare Fassung)}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2019}, language = {de} }