@article{ButenwegFaeckeFehlingetal.2010, author = {Butenweg, Christoph and F{\"a}cke, Andreas and Fehling, Ekkehard and Gellert, Christoph and Gierga, Michael and Meyer, Udo and R{\"u}tschlin, Andreas H. and St{\"u}rz, Jochen}, title = {Energieeffizient und erdbebensicher Bauen mit monolithischem Ziegelmauerwerk}, series = {Mauerwerk : European journal of masonry}, volume = {14}, journal = {Mauerwerk : European journal of masonry}, number = {1}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1432-3427}, doi = {10.1002/dama.201000456}, pages = {3 -- 9}, year = {2010}, abstract = {Moderne Bauwerke m{\"u}ssen heute eine hohe energetische Leistungsf{\"a}higkeit aufweisen und gleichzeitig alle einwirkenden Lasten sicher abtragen. Dies stellt insbesondere in Erdbebengebieten hohe Anforderungen an die verwendeten Baustoffe. Am baupraktischen Beispiel einer Doppelhaush{\"a}lfte wird demonstriert, dass die Symbiose aus energieeffizientem und gleichzeitig erdbebensicherem Bauen in der h{\"o}chsten deutschen Erdbebenzone mit monolithischem Ziegelmauerwerk gut realisierbar ist. Als Ziegelmauerwerk werden f{\"u}r die Außenw{\"a}nde w{\"a}rmetechnisch optimierte Hochlochziegel verwendet, die sowohl die Anforderungen der Energieeinsparverordnung 2009 als auch die Anforderungen an Mauerwerkbaustoffe nach den aktuellen Erdbebennormen erf{\"u}llen. Der Erdbebennachweis der Doppelhaush{\"a}lfte erfolgt mit einem nichtlinearen Nachweisverfahren, das f{\"u}r eine einfache praktische Anwendung programmtechnisch umgesetzt wurde. F{\"u}r den Nachweis wurden aus zyklischen Schubwandversuchen ermittelte Last-Verformungskurven verwendet. Das gesamte in Deutschland noch nicht normativ geregelte Nachweiskonzept wurde im Rahmen einer Zustimmung im Einzelfall gepr{\"u}ft und genehmigt.}, language = {de} } @article{ButenwegGellertSchlundt2010, author = {Butenweg, Christoph and Gellert, Christoph and Schlundt, Andreas}, title = {Nichtlinearer Erdbebennachweis von Geb{\"a}uden aus Kalksandsteinmauerwerk nach DIN EN 1998}, series = {Mauerwerk : European journal of masonry}, volume = {14}, journal = {Mauerwerk : European journal of masonry}, number = {3}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1432-3427}, doi = {10.1002/dama.201000463}, pages = {120 -- 125}, year = {2010}, abstract = {Die Erdbebensicherheit von Geb{\"a}uden aus Kalksandsteinmauerwerk ist aktuell nach DIN 4149 mit linearen Verfahren nachzuweisen. Dies f{\"u}hrt in der praktischen Anwendung zu großen Problemen, da selbst traditionell {\"u}bliche Grundrisse teilweise nicht mehr ohne zus{\"a}tzliche Maßnahmen nachweisbar sind. Zur L{\"o}sung dieser Problematik wurden von der deutschen Mauerwerksindustrie auf nationaler und europ{\"a}ischer Ebene Forschungsprojekte initiiert, deren Ergebnisse in Form von statisch nichtlinearen Verfahren Eingang in den Nationalen Anhang zur DIN EN 1998-1 [12] gefunden haben. Mit den Verfahren wird die Nachweissituation zuk{\"u}nftig grundlegend verbessert, da mit diesen die Tragwerksreserven wesentlich besser ausgenutzt werden k{\"o}nnen. Im Folgenden wird die Anwendung der Verfahren am Beispiel einer Reihenhauszeile aus Kalksandsteinmauerwerk demonstriert. Der Nachweis der Reihenh{\"a}user wurde im Rahmen einer Zustimmung im Einzelfall, die vom Bundesverband Kalksandsteinindustrie eV in Hannover koordiniert wurde, durch unabh{\"a}ngige Gutachter und die Bauaufsicht eingehend gepr{\"u}ft und f{\"u}r richtig befunden. Die Durchf{\"u}hrung des Nachweises erfolgte auf Grundlage eines an der RWTH Aachen entwickelten neuen Nachweiskonzeptes. Die baupraktische Anwendbarkeit und einfache Nachvollziehbarkeit dieses Konzepts ist durch eine softwaretechnische Umsetzung sichergestellt.}, language = {de} } @article{MottaghyPechnigTaugsetal.2010, author = {Mottaghy, Darius and Pechnig, Renate and Taugs, Renate and Kr{\"o}ger, Jens and Thomsen, Claudia and Hesse, Fabian and Liebsch-Doerschner, Thomas}, title = {Erstellung eines geothermischen Modells f{\"u}r Teile Hamburgs und anliegende Gebiete}, series = {BBR - Fachmagazin f{\"u}r Brunnen- und Leitungsbau}, volume = {61}, journal = {BBR - Fachmagazin f{\"u}r Brunnen- und Leitungsbau}, number = {12}, publisher = {WVGW Wirtschafts- u. Verl.Ges. Gas und Wasser}, address = {Bonn}, issn = {1611-1478}, pages = {52 -- 59}, year = {2010}, language = {de} } @article{VogtMottaghyWolfetal.2010, author = {Vogt, C. and Mottaghy, Darius and Wolf, A. and Rath, V. and Pechnig, R. and Clauser, C.}, title = {Reducing temperature uncertainties by stochastic geothermal reservoir modelling}, series = {Geophysical Journal International}, volume = {181}, journal = {Geophysical Journal International}, number = {1}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1365-246X}, doi = {10.1111/j.1365-246X.2009.04498.x}, pages = {321 -- 333}, year = {2010}, abstract = {Quantifying and minimizing uncertainty is vital for simulating technically and economically successful geothermal reservoirs. To this end, we apply a stochastic modelling sequence, a Monte Carlo study, based on (i) creating an ensemble of possible realizations of a reservoir model, (ii) forward simulation of fluid flow and heat transport, and (iii) constraining post-processing using observed state variables. To generate the ensemble, we use the stochastic algorithm of Sequential Gaussian Simulation and test its potential fitting rock properties, such as thermal conductivity and permeability, of a synthetic reference model and—performing a corresponding forward simulation—state variables such as temperature. The ensemble yields probability distributions of rock properties and state variables at any location inside the reservoir. In addition, we perform a constraining post-processing in order to minimize the uncertainty of the obtained distributions by conditioning the ensemble to observed state variables, in this case temperature. This constraining post-processing works particularly well on systems dominated by fluid flow. The stochastic modelling sequence is applied to a large, steady-state 3-D heat flow model of a reservoir in The Hague, Netherlands. The spatial thermal conductivity distribution is simulated stochastically based on available logging data. Errors of bottom-hole temperatures provide thresholds for the constraining technique performed afterwards. This reduce the temperature uncertainty for the proposed target location significantly from 25 to 12 K (full distribution width) in a depth of 2300 m. Assuming a Gaussian shape of the temperature distribution, the standard deviation is 1.8 K. To allow a more comprehensive approach to quantify uncertainty, we also implement the stochastic simulation of boundary conditions and demonstrate this for the basal specific heat flow in the reservoir of The Hague. As expected, this results in a larger distribution width and hence, a larger, but more realistic uncertainty estimate. However, applying the constraining post-processing the uncertainty is again reduced to the level of the post-processing without stochastic boundary simulation. Thus, constraining post-processing is a suitable tool for reducing uncertainty estimates by observed state variables.}, language = {en} } @article{Kirsch2010, author = {Kirsch, Ansgar}, title = {Experimental investigation of the face stability of shallow tunnels in sand}, series = {Acta Geotechnica}, volume = {5}, journal = {Acta Geotechnica}, number = {1}, publisher = {Springer}, address = {Berlin}, issn = {1861-1125}, doi = {10.1007/s11440-010-0110-7}, pages = {43 -- 62}, year = {2010}, abstract = {Various models have been proposed for the prediction of the necessary support pressure at the face of a shallow tunnel. To assess their quality, the collapse of a tunnel face was modelled with small-scale model tests at single gravity. The development of the failure mechanism and the support force at the face in dry sand were investigated. The observed displacement patterns show a negligible influence of overburden on the extent and evolution of the failure zone. The latter is significantly influenced, though, by the initial density of the sand: in dense sand a chimney-wedge-type collapse mechanism developed, which propagated towards the soil surface. Initially, loose sand did not show any discrete collapse mechanism. The necessary support force was neither influenced by the overburden nor the initial density. A comparison with quantitative predictions by several theoretical models showed that the measured necessary support pressure is overestimated by most of the models. Those by Vermeer/Ruse and L{\´e}ca/Dormieux showed the best agreement to the measurements.}, language = {en} } @article{FellinKingKirschetal.2010, author = {Fellin, Wolfgang and King, Julian and Kirsch, Ansgar and Oberguggenberger, Michael}, title = {Uncertainty modelling and sensitivity analysis of tunnel face stability}, series = {Structural safety}, volume = {32}, journal = {Structural safety}, number = {6}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0167-4730}, doi = {10.1016/j.strusafe.2010.06.001}, pages = {402 -- 410}, year = {2010}, abstract = {This paper proposes an approach to the choice and evaluation of engineering models with the aid of a typical application in geotechnics. An important issue in the construction of shallow tunnels, especially in weak ground conditions, is the tunnel face stability. Various theoretical and numerical models for predicting the necessary support pressure have been put forth in the literature. In this paper, we combine laboratory experiments performed at the University of Innsbruck with current methods of uncertainty and sensitivity analysis for assessing adequacy, predictive power and robustness of the models. The major issues are the handling of the twofold uncertainty of test results and of model predictions as well as the decision about what are the influential input parameters.}, language = {en} } @article{BolikLinzbach2010, author = {Bolik, Andreas and Linzbach, Meike}, title = {Verluste und Zinsschranke in der Bilanzierung latenter Steuern}, series = {Deutsches Steuerrecht : DStR ; Wochenschrift \& umfassende Datenbank f{\"u}r Steuerberater ; Steuerrecht, Wirtschaftsrecht, Betriebswirtschaft, Beruf ; Organ der Bundessteuerberaterkammer}, volume = {48}, journal = {Deutsches Steuerrecht : DStR ; Wochenschrift \& umfassende Datenbank f{\"u}r Steuerberater ; Steuerrecht, Wirtschaftsrecht, Betriebswirtschaft, Beruf ; Organ der Bundessteuerberaterkammer}, number = {31}, publisher = {Beck}, address = {M{\"u}nchen}, issn = {0012-1347}, pages = {1587 -- 1590}, year = {2010}, abstract = {Latente Steuern stellen eine Schnittstelle zwischen handelsrechtlicher Rechnungslegung und Steuerrecht dar. Das Verh{\"a}ltnis von Handels- und Steuerrecht ist dabei keinesfalls stetig; vielmehr fordert die Dynamik des deutschen Steuerrechts in vielen Bereichen regelm{\"a}ßige Anpassungen in der Bilanzierung in Handels- und Steuerbilanz. Dies gilt insbesondere f{\"u}r die Bilanzierung latenter Steuern im handelsrechtlichen Jahresabschluss, welche durch das BilMoG deutlich an Bedeutung gewonnen hat. Neben latenten Steuern auf tempor{\"a}re Differenzen ist es nunmehr erforderlich, Verlustvortr{\"a}ge und die Folgen der Zinsschranke bei der Berechnung latenter Steuern zu ber{\"u}cksichtigen. Der nachfolgende Beitrag geht auf die Ber{\"u}cksichtigung von Verlust- und Zinsvortr{\"a}gen und die Ber{\"u}cksichtigungsf{\"a}higkeit von EBITDA-Vortr{\"a}gen bei der Berechnung latenter Steuern ein und beleuchtet dabei insbesondere auch praktische Fragestellungen.}, language = {de} } @article{BenzelLinzbach2010, author = {Benzel, Ute and Linzbach, Meike}, title = {Bilanzierung und Offenlegung von Ertragsteuerrisiken - Quo vadis? Unsichere Steuerpositionen in der Rechnungslegung nach IFRS, US-GAAP und HGB}, series = {IRZ - Zeitschrift f{\"u}r Internationale Rechnungslegung}, journal = {IRZ - Zeitschrift f{\"u}r Internationale Rechnungslegung}, number = {11}, issn = {1862-5533}, pages = {475 -- 523}, year = {2010}, abstract = {In den letzten drei bis vier Jahren unterlag der Bereich der Bilanzierung und Offenlegung von unsicheren Steuerpositionen im IFRS- und US-GAAP-Abschluss einem stetigen Wandel. Sowohl nach US-GAAP als auch nach IFRS nehmen die Anforderungen an die Bilanzierung und Offenlegung best{\"a}ndig zu. Schon allein dies zeigt die Bedeutung, die dem Themenbereich der Steuerrisiken beigemessen wird. Aber nicht nur im Rahmen der Berichterstattung im Jahresabschluss stehen Steuerrisiken oben auf der Agenda, auch der amerikanische Internal Revenue Service (IRS) verfolgt das Thema sehr konsequent und plant, bestimmte Unternehmen zu Angaben von Steuerrisiken im Rahmen der Steuererkl{\"a}rungen zu verpflichten. Der Aufsatz befasst sich mit den neueren Entwicklungen im Bereich der Bilanzierung und Offenlegung von unsicheren Steuerpositionen in der Rechnungslegung nach IFRS, US-GAAP und HGB und der Offenlegung von Steuerrisiken im Rahmen der Steuererkl{\"a}rung.}, language = {de} } @article{WollertLeidinger2010, author = {Wollert, J{\"o}rg and Leidinger, Rafael}, title = {Automation trifft Automotive : SPS in der Fahrzeugtechnik}, series = {Elektronik automotive : Magazin f{\"u}r Entwicklungen in der Kfz-Elektronik und Telematik}, journal = {Elektronik automotive : Magazin f{\"u}r Entwicklungen in der Kfz-Elektronik und Telematik}, number = {4/5}, publisher = {WEKA Fachmedien}, address = {Haar}, issn = {1614-0125}, pages = {44 -- 48}, year = {2010}, abstract = {Eine SPS im Kraftfahrzeug? Das geht ja gar nicht - da gibt es doch all die Hersteller f{\"u}r Kfz-Steuerger{\"a}te, die konfigurierbare Hardware anbieten. Im Prinzip ja, aber leider sind gerade die Sensoren oder Aktoren nicht ansteuerbar, die der Entwickler genau f{\"u}r sein aktuelles Projekt ben{\"o}tigt. Dieser Beitrag gibt einen kleinen Einblick in neue M{\"o}glichkeiten des Rapid Prototyping f{\"u}r mechatronische Systeme auf der Basis von Speicherprogrammierbaren Steuerungen (SPS).}, language = {de} } @article{BragardSoltauThomasetal.2010, author = {Bragard, Michael and Soltau, N. and Thomas, S. and De Doncker, R. W.}, title = {The balance of renewable sources and user demands in grids : power electronics for modular battery energy storage systems}, series = {IEEE transactions on power electronics}, volume = {25}, journal = {IEEE transactions on power electronics}, number = {12}, publisher = {IEEE}, address = {New York}, issn = {0885-8993}, doi = {10.1109/TPEL.2010.2085455}, pages = {3049 -- 3056}, year = {2010}, abstract = {The continuously growing amount of renewable sources starts compromising the stability of electrical grids. Contradictory to fossil fuel power plants, energy production of wind and photovoltaic (PV) energy is fluctuating. Although predictions have significantly improved, an outage of multi-MW offshore wind farms poses a challenging problem. One solution could be the integration of storage systems in the grid. After a short overview, this paper focuses on two exemplary battery storage systems, including the required power electronics. The grid integration, as well as the optimal usage of volatile energy reserves, is presented for a 5- kW PV system for home application, as well as for a 100- MW medium-voltage system, intended for wind farm usage. The efficiency and cost of topologies are investigated as a key parameter for large-scale integration of renewable power at medium- and low-voltage.}, language = {en} } @article{EilmannBuchmannSiegwolfetal.2010, author = {Eilmann, Britta and Buchmann, Nina and Siegwolf, Rolf and Saurer, Matthias and Cherubini, Paolo and Rigling, Andreas}, title = {Fast response of Scots pine to improved water availability reflected in tree-ring width and δ13C}, series = {Plant, Cell and Environment}, volume = {33}, journal = {Plant, Cell and Environment}, number = {8}, publisher = {Wiley-Blackwell}, address = {Oxford}, issn = {1365-3040 (Online)}, doi = {10.1111/j.1365-3040.2010.02153.x}, pages = {1351 -- 1360}, year = {2010}, abstract = {Drought-induced forest decline, like the Scots pine mortality in inner-Alpine valleys, will gain in importance as the frequency and severity of drought events are expected to increase. To understand how chronic drought affects tree growth and tree-ring δ13C values, we studied mature Scots pine in an irrigation experiment in an inner-Alpine valley. Tree growth and isotope analyses were carried out at the annual and seasonal scale. At the seasonal scale, maximum δ13C values were measured after the hottest and driest period of the year, and were associated with decreasing growth rates. Inter-annual δ13C values in early- and latewood showed a strong correlation with annual climatic conditions and an immediate decrease as a response to irrigation. This indicates a tight coupling between wood formation and the freshly produced assimilates for trees exposed to chronic drought. This rapid appearance of the isotopic signal is a strong indication for an immediate and direct transfer of newly synthesized assimilates for biomass production. The fast appearance and the distinct isotopic signal suggest a low availability of old stored carbohydrates. If this was a sign for C-storage depletion, an increasing mortality could be expected when stressors increase the need for carbohydrate for defence, repair or regeneration.}, language = {en} } @article{EgliAyerPeteretal.2010, author = {Egli, Simon and Ayer, Fran{\c{c}}ois and Peter, Martina and Eilmann, Britta and Rigling, Andreas}, title = {Is forest mushroom productivity driven by tree growth? Results from a thinning experiment}, series = {Annals of Forest Science}, volume = {67}, journal = {Annals of Forest Science}, number = {5}, publisher = {Springer}, address = {Paris}, issn = {1286-4560 (Print)}, doi = {10.1051/forest/2010011}, pages = {509}, year = {2010}, abstract = {• Most of the edible forest mushrooms are mycorrhizal and depend on carbohydrates produced by the associated trees. Fruiting patterns of these fungi are not yet fully understood since climatic factors alone do not completely explain mushroom occurrence. • The objective of this study was to retrospectively find out if changing tree growth following an increment thinning has influenced the diversity patterns and productivity of associated forest mushrooms in the fungus reserve La Chan{\´e}az, Switzerland. • The results reveal a clear temporal relationship between the thinning, the growth reaction of trees and the reaction of the fungal community, especially for the ectomycorrhizal species. The tree-ring width of the formerly suppressed beech trees and the fruit body number increased after thinning, leading to a significantly positive correlation between fruit body numbers and tree-ring width. • Fruit body production was influenced by previous annual tree growth, the best accordance was found between fruit body production and the tree-ring width two years previously. • The results support the hypothesis that ectomycorrhizal fruit body production must be linked with the growth of the associated host trees. Moreover, the findings indicate the importance of including mycorrhizal fungi as important players when discussing a tree as a carbon source or sink.}, language = {en} } @article{FontivonArxGarciaGonzalezetal.2010, author = {Fonti, Patrick and von Arx, Georg and Garc{\´i}a-Gonz{\´a}lez, Ignacio and Eilmann, Britta and Sass-Klaassen, Ute G. W. and G{\"a}rtner, Holger and Eckstein, Dieter}, title = {Studying global change through investigation of the plastic responses of xylem anatomy in tree rings}, series = {New Phytologist}, volume = {185}, journal = {New Phytologist}, number = {1}, publisher = {Wiley-Blackwell}, address = {Oxford}, issn = {1469-8137 (Online)}, doi = {10.1111/j.1469-8137.2009.03030.x}, pages = {42 -- 53}, year = {2010}, abstract = {Variability in xylem anatomy is of interest to plant scientists because of the role water transport plays in plant performance and survival. Insights into plant adjustments to changing environmental conditions have mainly been obtained through structural and functional comparative studies between taxa or within taxa on contrasting sites or along environmental gradients. Yet, a gap exists regarding the study of hydraulic adjustments in response to environmental changes over the lifetimes of plants. In trees, dated tree-ring series are often exploited to reconstruct dynamics in ecological conditions, and recent work in which wood-anatomical variables have been used in dendrochronology has produced promising results. Environmental signals identified in water-conducting cells carry novel information reflecting changes in regional conditions and are mostly related to short, sub-annual intervals. Although the idea of investigating environmental signals through wood anatomical time series goes back to the 1960s, it is only recently that low-cost computerized image-analysis systems have enabled increased scientific output in this field. We believe that the study of tree-ring anatomy is emerging as a promising approach in tree biology and climate change research, particularly if complemented by physiological and ecological studies. This contribution presents the rationale, the potential, and the methodological challenges of this innovative approach.}, language = {en} } @article{DobbertinEilmannBleuleretal.2010, author = {Dobbertin, Matthias and Eilmann, Britta and Bleuler, Peter and Giuggiola, Arnaud and Graf Pannatier, Elisabeth and Landolt, Werner and Schleppi, Patrick and Rigling, Andreas}, title = {Effect of irrigation on needle morphology, shoot and stem growth in a drought-exposed Pinus sylvestris forest}, series = {Tree Physiology}, volume = {30}, journal = {Tree Physiology}, number = {3}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1758-4469 (Online)}, doi = {10.1093/treephys/tpp123}, pages = {346 -- 360}, year = {2010}, abstract = {In Valais, Switzerland, Scots pines (Pinus sylvestris L.) are declining, mainly following drought. To assess the impact of drought on tree growth and survival, an irrigation experiment was initiated in 2003 in a mature pine forest, approximately doubling the annual precipitation. Tree crown transparency (lack of foliage) and leaf area index (LAI) were annually assessed. Seven irrigated and six control trees were felled in 2006, and needles, stem discs and branches were taken for growth analysis. Irrigation in 2004 and 2005, both with below-average precipitation, increased needle size, area and mass, stem growth and, with a 1-year delay, shoot length. This led to a relative decrease in tree crown transparency (-14\%) and to an increase in stand LAI (+20\%). Irrigation increased needle length by 70\%, shoot length by 100\% and ring width by 120\%, regardless of crown transparency. Crown transparency correlated positively with mean needle size, shoot length and ring width and negatively with specific leaf area. Trees with high crown transparency (low growth, short needles) experienced similar increases in needle mass and growth with irrigation than trees with low transparency (high growth, long needles), indicating that seemingly declining trees were able to 'recover' when water supply became sufficient. A simple drought index before and during the irrigation explained most of the variation found in the parameters for both irrigated and control trees.}, language = {en} } @article{RiglingEilmannKoechlietal.2010, author = {Rigling, Andreas and Eilmann, Britta and Koechli, Roger and Dobbertin, Matthias}, title = {Mistletoe-induced crown degradation in Scots pine in a xeric environment}, volume = {30}, number = {7}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1758-4469 (Online)}, doi = {10.1093/treephys/tpq038}, pages = {845 -- 832}, year = {2010}, abstract = {Increasing Scots pine (Pinus sylvestris L.) mortality has been recently observed in the dry inner valleys of the European Alps. Besides drought, infection with pine mistletoe (Viscum album ssp. austriacum) seems to play an important role in the mortality dynamics of Scots pines, but how mistletoes promote pine decline remains unclear. To verify whether pine mistletoe infection weakens the host via crown degradation, as observed for dwarf mistletoes, we studied the negative effects of pine mistletoe infestation on the photosynthetic tissues and branch growth of pairs of infested and non-infested branches. Pine mistletoe infection leads to crown degradation in its host by reducing the length, the radial increment, the ramification, the needle length and the number of needle years of the infested branches. This massive loss in photosynthetic tissue results in a reduction in primary production and a subsequent decrease in carbohydrate availability. The significant reduction in needle length due to mistletoe infection is an indication for a lower water and nutrient availability in infested branches. Thus, mistletoe infection might lead to a decrease in the availability of water and carbohydrates, the two most important growth factors, which are already shortened due to the chronic drought situation in the area. Therefore, pine mistletoe increases the risk of drought-induced mortality of its host when growing in a xeric environment.}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{FerreinNiemuellerSteinbauer2010, author = {Ferrein, Alexander and Niem{\"u}ller, Tim and Steinbauer, Gerald}, title = {Team Zadeat 2010 : application for participation}, pages = {5 Seiten}, year = {2010}, language = {en} } @article{KuhnhenneDoeringFeldmann2010, author = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Feldmann, Markus}, title = {Grunds{\"a}tze und L{\"o}sungen zur W{\"a}rmebr{\"u}ckenreduktion im Metallleichtbau}, series = {Stahlbau}, volume = {79}, journal = {Stahlbau}, number = {5}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1437-1049 (E-Journal); 0038-9145 (Print)}, doi = {10.1002/stab.201001330}, pages = {345 -- 355}, year = {2010}, abstract = {Die Anforderungen an das energiesparende Bauen sind mit der Einf{\"u}hrung der Energieeinsparverordnung (EnEV) 2009 auch im Industrie- und Gewerbebau deutlich versch{\"a}rft worden. Einen wesentlichen Beitrag zur Energieeinsparung liefert die Minimierung des Transmissionsw{\"a}rmetransfers. Analysiert man Geb{\"a}udeh{\"u}llen in Metallleichtbauweise stellt man fest, dass eine Erh{\"o}hung der W{\"a}rmed{\"a}mmst{\"a}rke allein noch nicht zielf{\"u}hrend ist, zus{\"a}tzlich sind W{\"a}rmebr{\"u}ckeneffekte zu ber{\"u}cksichtigen und deren Einfl{\"u}sse auf die W{\"a}rmetransmission zu reduzieren. Neben der Bedeutung f{\"u}r die Energieeinsparung ist eine w{\"a}rmetechnisch optimierte Detailausbildung auch erforderlich, um einen ausreichenden Feuchteschutz (Vermeidung von Tauwasser und Schimmelpilz) zu realisieren und so Sch{\"a}den zu vermeiden. Ein wichtiges Hilfsmittel stellt hierzu der vom Industrieverband f{\"u}r Bausysteme im Metallleichtbau (IFBS) herausgegebene W{\"a}rmebr{\"u}ckenatlas der Metall-Sandwichbauweise dar.}, language = {de} } @article{KuhnhenneDoeringKockeretal.2010, author = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Kocker, Ronald and Pyschny, Dominik and Feldmann, Markus}, title = {Die {\"O}kobilanz als Baustein der Nachhaltigkeitsbewertung im Industrie- und Gewerbebau}, series = {Stahlbau}, volume = {79}, journal = {Stahlbau}, number = {6}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1437-1049 (E-Journal); 0038-9145 (Print)}, doi = {10.1002/stab.201001336}, pages = {439 -- 447}, year = {2010}, abstract = {Im Herbst 2009 wurde das "Deutsche G{\"u}tesiegel Nachhaltiges Bauen" f{\"u}r die Geb{\"a}udetypen Industriebauten und Handelsbauten offiziell vorgestellt. Die {\"O}kobilanz als wichtiger Baustein der Nachhaltigkeitsbewertung wird somit auch im Industrie- und Gewerbebau zuk{\"u}nftig stark an Bedeutung gewinnen. Der vorliegende Beitrag beinhaltet einen Vergleich der Umweltleistung verschiedener Bauweisen des Industrie- und Gewerbebaus, die durch die {\"O}kobilanzierung quantifizierbar wird, anhand von momentan verf{\"u}gbaren Daten und Methoden. Es werden ausgew{\"a}hlte Ergebnisse sowie vorhandene Unsicherheiten und daraus resultierender Forschungsbedarf aufgezeigt.}, language = {de} } @article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} }