@article{KnoedlerRuehlEmontsetal.2019,
  author    = {Kn{\"o}dler, Matthias and R{\"u}hl, Clemens and Emonts, Jessica and Buyel, Johannes Felix},
  title     = {Seasonal weather changes affect the yield and quality of recombinant proteins produced in transgenic tobacco plants in a greenhouse setting},
  series = {Frontiers in Plant Science},
  journal   = {Frontiers in Plant Science},
  number    = {10},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {1664-462X (online-ressource)},
  doi       = {10.3389/fpls.2019.01245},
  pages     = {13 Seiten},
  year      = {2019},
  abstract  = {Transgenic plants have the potential to produce recombinant proteins on an agricultural scale, with yields of several tons per year. The cost-effectiveness of transgenic plants increases if simple cultivation facilities such as greenhouses can be used for production. In such a setting, we expressed a novel affinity ligand based on the fluorescent protein DsRed, which we used as a carrier for the linear epitope ELDKWA from the HIV-neutralizing antibody 2F5. The DsRed-2F5-epitope (DFE) fusion protein was produced in 12 consecutive batches of transgenic tobacco (Nicotiana tabacum) plants over the course of 2 years and was purified using a combination of blanching and immobilized metal-ion affinity chromatography (IMAC). The average purity after IMAC was 57 ± 26\% (n = 24) in terms of total soluble protein, but the average yield of pure DFE (12 mg kg-1) showed substantial variation (± 97 mg kg-1, n = 24) which correlated with seasonal changes. Specifically, we found that temperature peaks (>28°C) and intense illuminance (>45 klx h-1) were associated with lower DFE yields after purification, reflecting the loss of the epitope-containing C-terminus in up to 90\% of the product. Whereas the weather factors were of limited use to predict product yields of individual harvests conducted for each batch (spaced by 1 week), the average batch yields were well approximated by simple linear regression models using two independent variables for prediction (illuminance and plant age). Interestingly, accumulation levels determined by fluorescence analysis were not affected by weather conditions but positively correlated with plant age, suggesting that the product was still expressed at high levels, but the extreme conditions affected its stability, albeit still preserving the fluorophore function. The efficient production of intact recombinant proteins in plants may therefore require adequate climate control and shading in greenhouses or even cultivation in fully controlled indoor farms.},
  language  = {en}
}
@phdthesis{Emonts2013,
  author    = {Emonts, Jessica},
  title     = {Searching for many defective edges in hypergraphs},
  publisher = {Rheinisch-Westf{\"a}lischen Technischen Hochschule Aachen},
  address   = {Aachen},
  pages     = {VIII, 104 Seiten : Ill.},
  year      = {2013},
  language  = {en}
}
@article{BernauKnoedlerEmontsetal.2022,
  author    = {Bernau, C. R. and Kn{\"o}dler, Matthias and Emonts, Jessica and J{\"a}pel, Ronald Colin and Buyel, Johannes Felix},
  title     = {The use of predictive models to develop chromatography-based purification processes},
  series = {Frontiers in Bioengineering and Biotechnology},
  journal   = {Frontiers in Bioengineering and Biotechnology},
  number    = {10},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2296-4185 (online-ressource)},
  doi       = {10.3389/fbioe.2022.1009102},
  pages     = {25 Seiten},
  year      = {2022},
  abstract  = {Chromatography is the workhorse of biopharmaceutical downstream processing because it can selectively enrich a target product while removing impurities from complex feed streams. This is achieved by exploiting differences in molecular properties, such as size, charge and hydrophobicity (alone or in different combinations). Accordingly, many parameters must be tested during process development in order to maximize product purity and recovery, including resin and ligand types, conductivity, pH, gradient profiles, and the sequence of separation operations. The number of possible experimental conditions quickly becomes unmanageable. Although the range of suitable conditions can be narrowed based on experience, the time and cost of the work remain high even when using high-throughput laboratory automation. In contrast, chromatography modeling using inexpensive, parallelized computer hardware can provide expert knowledge, predicting conditions that achieve high purity and efficient recovery. The prediction of suitable conditions in silico reduces the number of empirical tests required and provides in-depth process understanding, which is recommended by regulatory authorities. In this article, we discuss the benefits and specific challenges of chromatography modeling. We describe the experimental characterization of chromatography devices and settings prior to modeling, such as the determination of column porosity. We also consider the challenges that must be overcome when models are set up and calibrated, including the cross-validation and verification of data-driven and hybrid (combined data-driven and mechanistic) models. This review will therefore support researchers intending to establish a chromatography modeling workflow in their laboratory.},
  language  = {en}
}
@article{EmontsBuyel2023,
  author    = {Emonts, Jessica and Buyel, Johannes Felix},
  title     = {An overview of descriptors to capture protein properties - Tools and perspectives in the context of QSAR modeling},
  series = {Computational and Structural Biotechnology Journal},
  journal   = {Computational and Structural Biotechnology Journal},
  number    = {21},
  publisher = {Research Network of Computational and Structural Biotechnology},
  address   = {Gotenburg},
  issn      = {2001-0370 (online-ressource)},
  doi       = {10.1016/j.csbj.2023.05.022},
  pages     = {3234 -- 3247},
  year      = {2023},
  abstract  = {Proteins are important ingredients in food and feed, they are the active components of many pharmaceutical products, and they are necessary, in the form of enzymes, for the success of many technical processes. However, production can be challenging, especially when using heterologous host cells such as bacteria to express and assemble recombinant mammalian proteins. The manufacturability of proteins can be hindered by low solubility, a tendency to aggregate, or inefficient purification. Tools such as in silico protein engineering and models that predict separation criteria can overcome these issues but usually require the complex shape and surface properties of proteins to be represented by a small number of quantitative numeric values known as descriptors, as similarly used to capture the features of small molecules. Here, we review the current status of protein descriptors, especially for application in quantitative structure activity relationship (QSAR) models. First, we describe the complexity of proteins and the properties that descriptors must accommodate. Then we introduce descriptors of shape and surface properties that quantify the global and local features of proteins. Finally, we highlight the current limitations of protein descriptors and propose strategies for the derivation of novel protein descriptors that are more informative.},
  language  = {en}
}
@article{RakeEnningKurthetal.1994,
  author    = {Rake, Heinrich and Enning, Manfred and Kurth, Johannes and Schr{\"o}der, Walter},
  title     = {Automatic uncoupler completes automation at the hump},
  series = {RGI - Railway Gazette International},
  volume    = {150},
  journal   = {RGI - Railway Gazette International},
  number    = {6},
  publisher = {Reed Business},
  address   = {Sutton},
  issn      = {0373-5346},
  pages     = {371 -- 374},
  year      = {1994},
  language  = {en}
}
@article{RakeSchwanhaeusserFrederichetal.1993,
  author    = {Rake, Heinrich and Schwanh{\"a}ußer, Wulf and Frederich, Fritz and Enning, Manfred},
  title     = {Automatisierung von Ablaufanlagen mit dem Kupplungsroboter},
  series = {ETR - Eisenbahntechnische Rundschau},
  journal   = {ETR - Eisenbahntechnische Rundschau},
  number    = {4},
  publisher = {DVV Media Group},
  address   = {Hamburg},
  issn      = {0013-2845},
  pages     = {249 -- 254},
  year      = {1993},
  language  = {de}
}
@inproceedings{EnningHilgers2023,
  author    = {Enning, Manfred and Hilgers, Rudolf},
  title     = {Die Assistierte Bremsprobe als Br{\"u}cke zur Vollautomatisierung des Schieneng{\"u}terverkehrs},
  series = {IRSA 2023: Tagungsband, Proceedings},
  booktitle = {IRSA 2023: Tagungsband, Proceedings},
  editor    = {Nießen, Nils and Schindler, Christian},
  publisher = {RWTH Aachen},
  address   = {Aachen},
  doi       = {10.18154/RWTH-2024-00257},
  pages     = {60 -- 75},
  year      = {2023},
  language  = {de}
}
@article{ManfredMaySutter2020,
  author    = {Manfred, Enning and May, J{\"o}rg and Sutter, Stefan},
  title     = {Innovation am G{\"u}terwagen am Beispiel der Automatischen Bremsprobe},
  series = {ETR - Eisenbahntechnische Rundschau},
  journal   = {ETR - Eisenbahntechnische Rundschau},
  number    = {12},
  publisher = {DVV Media Group},
  address   = {Hamburg},
  issn      = {0013-2845},
  pages     = {14 -- 19},
  year      = {2020},
  abstract  = {Mit der Digitalen Automatischen Kupplung beginnt ein neues Kapitel des Schieneng{\"u}terverkehrs, in dem zusammengestellte Wagen sich automatisch in wenigen Minuten abfahrbereit machen, ohne dass der Mensch eingreifen muss. Eines des gr{\"o}ßten Hemmnisse der umweltfreundlichen Schiene wird dann entfallen. Notwendig ist jetzt eine Diskussion {\"u}ber den Umfang und die Systemgrenzen der Automatischen Bremsprobe.},
  language  = {de}
}
@article{EnningSchmidtWilbring2019,
  author    = {Enning, Manfred and Schmidt, Bernd and Wilbring, Daniela},
  title     = {Auf dem Weg zur autonomen Anschlussbedienung},
  series = {Privatbahn-Magazin},
  journal   = {Privatbahn-Magazin},
  number    = {3},
  publisher = {Bahn Media},
  address   = {Suhlendorf},
  issn      = {1865-0163},
  pages     = {40 -- 43},
  year      = {2019},
  language  = {de}
}
@inproceedings{WilbringEnningPfaffetal.2019,
  author    = {Wilbring, Daniela and Enning, Manfred and Pfaff, Raphael and Schmidt, Bernd},
  title     = {Neue Perspektiven f{\"u}r die Bahn in der Produktions- und Distributionslogistik durch Prozessautomation},
  series = {IRSA 2019: Tagungsband, Proceedings},
  booktitle = {IRSA 2019: Tagungsband, Proceedings},
  editor    = {de Doncker, Rik W. and Nießen, Nils and Schindler, Christian},
  publisher = {RWTH Aachen},
  address   = {Aachen},
  doi       = {10.18154/RWTH-2020-00014},
  pages     = {128 -- 142},
  year      = {2019},
  abstract  = {Deutschland braucht mehr Eisenbahn um CO2-Emissionen aus dem Verkehr zu reduzieren. Sie muss zum R{\"u}ckgrat aktueller Logistikprozesse, z.B. bei Kaufmannsg{\"u}tern und E-Commerce, werden. Dies geht nicht ohne neuartige betriebliche Konzepte und eine Transformation des G{\"u}terwagens von einem „dummen St{\"u}ck Stahl" zu einem modernen Werkzeug der Logistik. Als „G{\"u}terwagen 4.0" wird ein kommunikativer und kooperativer G{\"u}terwagen verstanden, der die Voraussetzung zur Automatisierung aller Prozesse der Zugvorbereitung bereitstellt, sich aber ansonsten vollkommen kompatibel mit heutigen Betriebsverfahren im Hauptlauf pr{\"a}sentiert. Durch Kommunikation zwischen G{\"u}terwagen und umgebenden intelligenten Systemen im Sinne eines „Internet der Dinge" gelingt damit unter Anderem die Realisierung hoch effizienter Gleisanschlussverkehre, die der G{\"u}terbahn neue M{\"a}rkte abseits der klassisch bahn-affinen Verkehre erschließen und letztlich den Wandel zu einer nachhaltigen G{\"u}termobilit{\"a}t f{\"o}rdern.},
  language  = {de}
}
@article{EnningPfaff2017,
  author    = {Enning, Manfred and Pfaff, Raphael},
  title     = {G{\"u}terwagen 4.0 - Mehr als nur technischer Fortschritt},
  series = {Privatbahn-Magazin},
  journal   = {Privatbahn-Magazin},
  number    = {2},
  publisher = {Bahn Media},
  address   = {Suhlendorf},
  issn      = {1865-0163},
  pages     = {21 -- 25},
  year      = {2017},
  abstract  = {Lokomotiven sind dank modernster Konzepte der Antriebstechnik heute energiesparend und umweltfreundlich. Eine Ausr{\"u}stung mit Telematik und Assistenzfunktionen ist Standard. Auf der Strecke zeigt sich moderne Technik in Form elektronischer Stellwerke und Zugsicherungssysteme und in Rangier- und Abstellanlagen als EOW-Technik. Am G{\"u}terwagen hingegen ist der technische Fortschritt komplett vorbeigegangen. Auch beim modernsten Wagen (Abb. 1) ist die einzige „Automatik"-Funktion die zentral {\"u}ber die Hauptluftleitung (HL) versorgte und bet{\"a}tigte Luftbremse.},
  language  = {de}
}
@article{EnningPfaff2016,
  author    = {Enning, Manfred and Pfaff, Raphael},
  title     = {Digitalisierung bringt mehr G{\"u}ter auf die Schiene},
  series = {Sonderprojekte ATM/MTZ},
  volume    = {21},
  journal   = {Sonderprojekte ATM/MTZ},
  number    = {6 (suppl.)},
  publisher = {Springer Fachmedien},
  address   = {Wiesbaden},
  issn      = {2509-4610},
  doi       = {10.1007/s41491-016-0570-7},
  pages     = {34 -- 37},
  year      = {2016},
  language  = {de}
}
@article{EichlerBalcBremenetal.2024,
  author    = {Eichler, Fabian and Balc, Nicolae and Bremen, Sebastian and Nink, Philipp},
  title     = {Investigation of laser powder bed fusion parameters with respect to their influence on the thermal conductivity of 316L samples},
  series = {Journal of Manufacturing and Materials Processing},
  volume    = {8},
  journal   = {Journal of Manufacturing and Materials Processing},
  number    = {4},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2504-4494},
  doi       = {10.3390/jmmp8040166},
  pages     = {12 Seiten},
  year      = {2024},
  abstract  = {The thermal conductivity of components manufactured using Laser Powder Bed Fusion (LPBF), also called Selective Laser Melting (SLM), plays an important role in their processing. Not only does a reduced thermal conductivity cause residual stresses during the process, but it also makes subsequent processes such as the welding of LPBF components more difficult. This article uses 316L stainless steel samples to investigate whether and to what extent the thermal conductivity of specimens can be influenced by different LPBF parameters. To this end, samples are set up using different parameters, orientations, and powder conditions and measured by a heat flow meter using stationary analysis. The heat flow meter set-up used in this study achieves good reproducibility and high measurement accuracy, so that comparative measurements between the various LPBF influencing factors to be tested are possible. In summary, the series of measurements show that the residual porosity of the components has the greatest influence on conductivity. The degradation of the powder due to increased recycling also appears to be detectable. The build-up direction shows no detectable effect in the measurement series.},
  language  = {en}
}
@techreport{BarnatArntzBerneckeretal.2024,
  type      = {Working Paper},
  author    = {Barnat, Miriam and Arntz, Kristian and Bernecker, Andreas and Fissabre, Anke and Franken, Norbert and Goldbach, Daniel and H{\"u}ning, Felix and J{\"o}rissen, J{\"o}rg and Kirsch, Ansgar and Pettrak, J{\"u}rgen and Rexforth, Matthias and Josef, Rosenkranz and Terstegge, Andreas},
  title     = {Strategische Gestaltung von Studieng{\"a}ngen f{\"u}r die Zukunft: Ein kollaborativ entwickeltes Self-Assessment},
  series = {Hochschulforum Digitalisierung - Diskussionspapier},
  journal   = {Hochschulforum Digitalisierung - Diskussionspapier},
  publisher = {Stifterverband f{\"u}r die Deutsche Wissenschaft},
  address   = {Berlin},
  issn      = {2365-7081},
  pages     = {16 Seiten},
  year      = {2024},
  abstract  = {Das Diskussionspapier beschreibt einen Prozess an der FH Aachen zur Entwicklung und Implementierung eines Self-Assessment-Tools f{\"u}r Studieng{\"a}nge. Dieser Prozess zielte darauf ab, die Relevanz der Themen Digitalisierung, Internationalisierung und Nachhaltigkeit in Studieng{\"a}ngen zu st{\"a}rken. Durch Workshops und kollaborative Entwicklung mit Studiendekan:innen entstand ein Fragebogen, der zur Reflexion und strategischen Weiterentwicklung der Studieng{\"a}nge dient.},
  language  = {de}
}
@masterthesis{Ferraioli2023,
  type      = {Bachelor Thesis},
  author    = {Ferraioli, Luigi},
  title     = {Validierung einer Simulationsumgebung f{\"u}r Umfeldsensorik von Schienenfahrzeugen},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {49 Seiten},
  year      = {2023},
  abstract  = {Diese Bachelorarbeit befasst sich mit der digitalen Nachbildung eines Testgel{\"a}ndes sowie eines Schienenfahrzeugs in der Simulationsumgebung Gazebo. Der Schwerpunkt liegt auf der pr{\"a}zisen Abbildung der Umfeldsensorik anhand eines realen Schienenfahrzeuges. Ziel ist die Erzeugung {\"a}quivalenter Messdaten der Simulationsumgebung und des realen Schienenfahrzeuges unter gleichen Einsatzbedingungen. Dazu werden unterschiedliche Verfahren eingesetzt, um die Parameter der einzelnen Sensorik so zu konfigurieren, dass die Messergebnisse mit den Messdaten der realen Sensorik konvergieren. Die Ergebnisse der Messdaten zeigen, dass obwohl die Simulationsumgebung einige physikalische Materialeigenschaften nicht ber{\"u}cksichtigt, eine pr{\"a}zise Abbildung der Sensorik und geometrischen Strukturen des Testgel{\"a}ndes m{\"o}glich ist. Dar{\"u}ber hinaus erm{\"o}glicht die Kombination von Gazebo und ROS2 Integrationstests und die Entwicklung von Softwareanwendungen sowohl in der Simulation als auch auf dem realen Schienenfahrzeug. Eine realit{\"a}tsnahe und reproduzierbare Auswertung der Sensormessdaten der Simulationsumgebung f{\"u}r Schienenfahrzeuge ist somit realisierbar.},
  language  = {de}
}
@incollection{Dreschers2023,
  author    = {Dreschers, Martin},
  title     = {\S18 Grenz{\"u}berschreitende Eigenverwaltung},
  series = {HRI II - Handbuch Restrukturierung in der Insolvenz},
  booktitle = {HRI II - Handbuch Restrukturierung in der Insolvenz},
  editor    = {K{\"u}bler, Bruno M. and Bork, Reinhard and Pr{\"u}tting, Hanns},
  publisher = {RWS Verlag},
  address   = {K{\"o}ln},
  isbn      = {978-3-8145-2010-0 (print)},
  doi       = {10.15375/9783814558714-028},
  pages     = {524 -- 556},
  year      = {2023},
  language  = {de}
}
@incollection{Dreschers2023,
  author    = {Dreschers, Martin},
  title     = {\S25 Arbeitsrechtliche Probleme im Rahmen der Betriebsfortf{\"u}hrung},
  series = {Betriebsfortf{\"u}hrung in Restrukturierung und Insolvenz},
  booktitle = {Betriebsfortf{\"u}hrung in Restrukturierung und Insolvenz},
  editor    = {M{\"o}nning, Rolf-Dieter},
  publisher = {RWS Verlag},
  address   = {K{\"o}ln},
  isbn      = {978-3-8145-2012-4 (print)},
  doi       = {https://doi.org/10.15375/9783814558882-028},
  pages     = {947 -- 988},
  year      = {2023},
  language  = {de}
}
@article{AbbasBalcBremenetal.2022,
  author    = {Abbas, Karim and Balc, Nicolae and Bremen, Sebastian and Skupin, Marco},
  title     = {Crystallization and aging behavior of polyetheretherketone PEEK within rapid tooling and rubber molding},
  series = {Journal of Manufacturing and Materials Processing},
  volume    = {6},
  journal   = {Journal of Manufacturing and Materials Processing},
  number    = {5},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2504-4494},
  doi       = {10.3390/jmmp6050093},
  pages     = {1 -- 12},
  year      = {2022},
  abstract  = {In times of short product life cycles, additive manufacturing and rapid tooling are important methods to make tool development and manufacturing more efficient. High-performance polymers are the key to mold production for prototypes and small series. However, the high temperatures during vulcanization injection molding cause thermal aging and can impair service life. The extent to which the thermal stress over the entire process chain stresses the material and whether it leads to irreversible material aging is evaluated. To this end, a mold made of PEEK is fabricated using fused filament fabrication and examined for its potential application. The mold is heated to 200 ◦C, filled with rubber, and cured. A differential scanning calorimetry analysis of each process step illustrates the crystallization behavior and first indicates the material resistance. It shows distinct cold crystallization regions at a build chamber temperature of 90 ◦C. At an ambient temperature above Tg, crystallization of 30\% is achieved, and cold crystallization no longer occurs. Additional tensile tests show a decrease in tensile strength after ten days of thermal aging. The steady decrease in recrystallization temperature indicates degradation of the additives. However, the tensile tests reveal steady embrittlement of the material due to increasing crosslinking.},
  language  = {en}
}
@article{LuftLuftArntz2023,
  author    = {Luft, Angela and Luft, Nils and Arntz, Kristian},
  title     = {A basic description logic for service-oriented architecture in factory planning and operational control in the age of industry 4.0},
  series = {Applied Sciences},
  volume    = {2023},
  journal   = {Applied Sciences},
  number    = {13},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2076-3417},
  doi       = {10.3390/app13137610},
  pages     = {23 Seiten},
  year      = {2023},
  abstract  = {Manufacturing companies across multiple industries face an increasingly dynamic and unpredictable environment. This development can be seen on both the market and supply side. To respond to these challenges, manufacturing companies must implement smart manufacturing systems and become more flexible and agile. The flexibility in operational planning regarding the scheduling and sequencing of customer orders needs to be increased and new structures must be implemented in manufacturing systems' fundamental design as they constitute much of the operational flexibility available. To this end, smart and more flexible solutions for production planning and control (PPC) are developed. However, scheduling or sequencing is often only considered isolated in a predefined stable environment. Moreover, their orientation on the fundamental logic of the existing IT solutions and their applicability in a dynamic environment is limited. This paper presents a conceptual model for a task-based description logic that can be applied to factory planning, technology planning, and operational control. By using service-oriented architectures, the goal is to generate smart manufacturing systems. The logic is designed to allow for easy and automated maintenance. It is compatible with the existing resource and process allocation logic across operational and strategic factory and production planning.},
  language  = {en}
}
@article{AbbasHedwigBalcetal.2023,
  author    = {Abbas, Karim and Hedwig, Lukas and Balc, Nicolae and Bremen, Sebastian},
  title     = {Advanced FFF of PEEK: Infill strategies and material characteristics for rapid tooling},
  series = {Polymers},
  volume    = {2023},
  journal   = {Polymers},
  number    = {15},
  publisher = {MDPI},
  address   = {Basel},
  doi       = {10.3390/polym15214293},
  pages     = {Artikel 4293},
  year      = {2023},
  abstract  = {Traditional vulcanization mold manufacturing is complex, costly, and under pressure due to shorter product lifecycles and diverse variations. Additive manufacturing using Fused Filament Fabrication and high-performance polymers like PEEK offer a promising future in this industry. This study assesses the compressive strength of various infill structures (honeycomb, grid, triangle, cubic, and gyroid) when considering two distinct build directions (Z, XY) to enhance PEEK's economic and resource efficiency in rapid tooling. A comparison with PETG samples shows the behavior of the infill strategies. Additionally, a proof of concept illustrates the application of a PEEK mold in vulcanization. A peak compressive strength of 135.6 MPa was attained in specimens that were 100\% solid and subjected to thermal post-treatment. This corresponds to a 20\% strength improvement in the Z direction. In terms of time and mechanical properties, the anisotropic grid and isotropic cubic infill have emerged for use in rapid tooling. Furthermore, the study highlights that reducing the layer thickness from 0.15 mm to 0.1 mm can result in a 15\% strength increase. The study unveils the successful utilization of a room-temperature FFF-printed PEEK mold in vulcanization injection molding. The parameters and infill strategies identified in this research enable the resource-efficient FFF printing of PEEK without compromising its strength properties. Using PEEK in rapid tooling allows a cost reduction of up to 70\% in tool production.},
  language  = {en}
}