@article{DantismRoehlenSelmeretal.2019, author = {Dantism, Shahriar and R{\"o}hlen, Desiree and Selmer, Thorsten and Wagner, Torsten and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Quantitative differential monitoring of the metabolic activity of Corynebacterium glutamicum cultures utilizing a light-addressable potentiometric sensor system}, series = {Biosensors and Bioelectronics}, volume = {139}, journal = {Biosensors and Bioelectronics}, publisher = {Elsevier}, address = {Amsterdam}, doi = {10.1016/j.bios.2019.111332}, pages = {Artikel 111332}, year = {2019}, language = {en} } @article{DantismRoehlenWagneretal.2019, author = {Dantism, Shahriar and R{\"o}hlen, Desiree and Wagner, Torsten and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {A LAPS-based differential sensor for parallelized metabolism monitoring of various bacteria}, series = {Sensors}, volume = {19}, journal = {Sensors}, number = {21}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s19214692}, pages = {Artikel 4692}, year = {2019}, abstract = {Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.}, language = {en} } @article{DantismRoehlenWagneretal.2018, author = {Dantism, Shahriar and R{\"o}hlen, Desiree and Wagner, Torsten and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Optimization of Cell-Based Multi-Chamber LAPS Measurements Utilizing FPGA-Controlled Laser-Diode Modules}, series = {physica status solidi a : applications and materials sciences}, volume = {215}, journal = {physica status solidi a : applications and materials sciences}, number = {15}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201800058}, pages = {Article number 1800058}, year = {2018}, abstract = {A light-addressable potentiometric sensor (LAPS) is a field-effect-based potentiometric device, which detects concentration changes of an analyte solution on the sensor surface in a spatially resolved way. It uses a light source to generate electron-hole pairs inside the semiconductor, which are separated in the depletion region due to an applied bias voltage across the sensor structure and hence, a surface-potential-dependent photocurrent can be read out. However, depending on the beam angle of the light source, scattering effects can occur, which influence the recorded signal in LAPS-based differential measurements. To solve this problem, a novel illumination unit based on a field programmable gate array (FPGA) consisting of 16 small-sized tunable infrared laser-diode modules (LDMs) is developed. Due to the improved focus of the LDMs with a beam angle of only 2 mrad, undesirable scattering effects are minimized. Escherichia coli (E. coli) K12 bacteria are used as a test microorganism to study the extracellular acidification on the sensor surface. Furthermore, a salt bridge chamber is built up and integrated with the LAPS system enabling multi-chamber differential measurements with a single Ag/AgCl reference electrode.}, language = {en} } @article{DantismTakenagaWagneretal.2016, author = {Dantism, Shahriar and Takenaga, Shoko and Wagner, Patrick and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Determination of the extracellular acidification of Escherichia coli K12 with a multi-​chamber-​based LAPS system}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.201533043}, pages = {1479 -- 1485}, year = {2016}, abstract = {On-line monitoring of the metabolic activity of microorganisms involved in intermediate stages of biogas production plays an important role to avoid undesirable "down times" during the biogas production. In order to control this process, an on-chip differential measuring system based on the light-addressable potentiometric sensor (LAPS) principle combined with a 3D-printed multi-chamber structure has been realized. As a test microorganism, Escherichia coli K12 (E. coli K12) were used for cell-based measurements. Multi-chamber structures were developed to determine the metabolic activity of E. coli K12 in suspension for a different number of cells, responding to the addition of a constant or variable amount of glucose concentrations, enabling differential and simultaneous measurements.}, language = {en} } @article{DantismTakenagaWagneretal.2017, author = {Dantism, Shahriar and Takenaga, Shoko and Wagner, Torsten and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Differential imaging of the metabolism of bacteria and eukaryotic cells based on light-addressable potentiometric sensors}, series = {Electrochimica Acta}, volume = {246}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2017.05.196}, pages = {234 -- 241}, year = {2017}, abstract = {A light-addressable potentiometric sensor (LAPS) is a field-effect-based potentiometric sensor with an electrolyte/insulator/semiconductor (EIS) structure, which is able to monitor analyte concentrations of (bio-)chemical species in aqueous solutions in a spatially resolved way. Therefore, it is also an appropriate tool to record 2D-chemical images of concentration variations on the sensor surface. In the present work, two differential, LAPS-based measurement principles are introduced to determine the metabolic activity of Escherichia coli (E. coli) K12 and Chinese hamster ovary (CHO) cells as test microorganisms. Hereby, we focus on i) the determination of the extracellular acidification rate (ΔpH/min) after adding glucose solutions to the cell suspensions; and ii) recording the amplitude increase of the photocurrent (Iph) related to the produced acids from E. coli K12 bacteria and CHO cells on the sensor surface by 2D-chemical imaging. For this purpose, 3D-printed multi-chamber structures were developed and mounted on the planar sensor-chip surface to define four independent compartments, enabling differential measurements with varying cell concentrations. The differential concept allows eliminating unwanted drift effects and, with the four-chamber structures, measurements on the different cell concentrations were performed simultaneously, thus reducing also the overall measuring time.}, language = {en} } @article{DashevskyLanzlKotliar2011, author = {Dashevsky, Alexey V. and Lanzl, Ines M. and Kotliar, Konstantin}, title = {Non-penetrating intracanalicular partial trabeculectomy via the ostia of Schlemm's canal}, series = {Graefe's Archive for Clinical and Experimental Ophthalmology}, volume = {249}, journal = {Graefe's Archive for Clinical and Experimental Ophthalmology}, number = {4}, publisher = {Springer}, address = {Berlin}, issn = {0721-832x}, pages = {565 -- 573}, year = {2011}, language = {en} } @inproceedings{deHondePorstDigel2017, author = {de Honde, Lukas and Porst, Dariusz and Digel, Ilya}, title = {A randomized, observational thermographic study of the neck region before and after a physiotherapeutic intervention}, series = {2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West}, booktitle = {2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West}, editor = {Fischerauer, Alice}, publisher = {Universit{\"a}t Duisburg-Essen}, address = {Duisburg}, organization = {MedTech Symposium}, isbn = {978-3-9814801-9-1}, doi = {10.17185/duepublico/43984}, pages = {122 -- 123}, year = {2017}, language = {en} } @article{DefosseKleinschmidtSchmutzetal.2022, author = {Defosse, Jerome and Kleinschmidt, Joris and Schmutz, Axel and Loop, Torsten and Staat, Manfred and Gatzweiler, Karl-Heinz and Wappler, Frank and Schieren, Mark}, title = {Dental strain on maxillary incisors during tracheal intubation with double-lumen tubes and different laryngoscopy techniques - a blinded manikin study}, series = {Journal of Cardiothoracic and Vascular Anesthesia}, volume = {36}, journal = {Journal of Cardiothoracic and Vascular Anesthesia}, number = {8, Part B}, publisher = {Elsevier}, address = {New York, NY}, issn = {1053-0770}, doi = {10.1053/j.jvca.2022.02.017}, pages = {3021 -- 3027}, year = {2022}, language = {en} } @article{DelleHuckBaeckeretal.2015, author = {Delle, Lotta E. and Huck, Christina and B{\"a}cker, Matthias and M{\"u}ller, Frank and Grandthyll, Samuel and Jacobs, Karin and Lilischkis, Rainer and Vu, Xuan T. and Sch{\"o}ning, Michael Josef and Wagner, Patrick and Thoelen, Roland and Weil, Maryam and Ingebrandt, Sven}, title = {Impedimetric immunosensor for the detection of histamine based on reduced graphene oxide}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431863}, pages = {1327 -- 1334}, year = {2015}, language = {en} } @article{DemirciTrzewikLinderetal.2004, author = {Demirci, T. and Trzewik, J. and Linder, Peter and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: Real Time PCR Products and Suppliers by Comparison}, series = {Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2}, journal = {Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2}, isbn = {0932-4666}, pages = {1046 -- 1047}, year = {2004}, language = {en} } @article{DemirciTrzewikLinderetal.2004, author = {Demirci, T. and Trzewik, J. and Linder, Peter and Digel, Ilya and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: ITGB5 and p53 Responses as Quantified on the mRNA Level}, series = {Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2}, journal = {Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2}, isbn = {0932-4666}, pages = {1030 -- 1031}, year = {2004}, language = {en} } @article{DemirciKurulganDemirciTrzewiketal.2009, author = {Demirci, Taylan and Kurulgan Demirci, Eylem and Trzewik, J{\"u}rgen and Linder, Peter and Digel, Ilya and Artmann, Gerhard and Sakizli, Meral and Temiz Artmann, Ayseg{\"u}l}, title = {Gene expression profile analysis of 3T3/NIH fibroblasts after one hour mechanical stress}, series = {IUBMB Life. 61 (2009), H. 3}, journal = {IUBMB Life. 61 (2009), H. 3}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {1521-6543}, pages = {311 -- 312}, year = {2009}, language = {en} } @article{Digel2011, author = {Digel, Ilya}, title = {Primary thermosensory events in cells}, series = {Transient receptor potential channels / Md. Shahidul Islam, ed.}, journal = {Transient receptor potential channels / Md. Shahidul Islam, ed.}, publisher = {Springer}, address = {Dordrecht [u.a.]}, isbn = {978-94-007-0264-6}, pages = {451 -- 468}, year = {2011}, language = {en} } @article{Digel2008, author = {Digel, Ilya}, title = {Controlling microbial adhesion : a surface engineering approach}, series = {Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)}, journal = {Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-540-75408-4}, pages = {601 -- 625}, year = {2008}, language = {en} } @article{Digel2010, author = {Digel, Ilya}, title = {In-situ biological decontamination of an ice melting probe}, year = {2010}, language = {en} } @book{Digel1998, author = {Digel, Ilya}, title = {Enhancement of microbial adhesion onto solid surfaces using transition metals and water-soluble polymers : academic PhD dissertation in microbiology, Division of Applied Microbiology, Department of Microbiology, Biology Faculty, Kazakh National State University, Almaty, Kazakhstan / Ilya Digel}, address = {Almaty}, year = {1998}, language = {en} } @incollection{DigelAkimbekovKistaubayevaetal.2018, author = {Digel, Ilya and Akimbekov, Nuraly S. and Kistaubayeva, Aida and Zhubanova, Azhar A.}, title = {Microbial Sampling from Dry Surfaces: Current Challenges and Solutions}, series = {Biological, Physical and Technical Basics of Cell Engineering}, booktitle = {Biological, Physical and Technical Basics of Cell Engineering}, editor = {Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l and Zhubanova, Azhar A. and Digel, Ilya}, publisher = {Springer}, address = {Singapore}, isbn = {978-981-10-7904-7}, doi = {10.1007/978-981-10-7904-7_19}, pages = {421 -- 456}, year = {2018}, abstract = {Sampling of dry surfaces for microorganisms is a main component of microbiological safety and is of critical importance in many fields including epidemiology, astrobiology as well as numerous branches of medical and food manufacturing. Aspects of biofilm formation, analysis and removal in aqueous solutions have been thoroughly discussed in literature. In contrast, microbial communities on air-exposed (dry) surfaces have received significantly less attention. Diverse surface sampling methods have been developed in order to address various surfaces and microbial groups, but they notoriously show poor repeatability, low recovery rates and suffer from lack of mutual consistency. Quantitative sampling for viable microorganisms represents a particular challenge, especially on porous and irregular surfaces. Therefore, it is essential to examine in depth the factors involved in microorganisms' recovery efficiency and accuracy depending on the sampling technique used. Microbial colonization, retention and community composition on different dry surfaces are very complex and rely on numerous physicochemical and biological factors. This study is devoted to analyze and review the (a) physical phenomena and intermolecular forces relevant for microbiological surface sampling; (b) challenges and problems faced by existing sampling methods for viable microorganisms and (c) current directions of engineering and research aimed at improvement of quality and efficiency of microbiological surface sampling.}, language = {en} } @article{DigelAkimbekovRogachevetal.2023, author = {Digel, Ilya and Akimbekov, Nuraly S. and Rogachev, Evgeniy and Pogorelova, Natalia}, title = {Bacterial cellulose produced by Medusomyces gisevii on glucose and sucrose: biosynthesis and structural properties}, series = {Cellulose}, journal = {Cellulose}, publisher = {Springer Science + Business Media}, address = {Dordrecht}, issn = {1572-882X (Online)}, doi = {10.1007/s10570-023-05592-z}, pages = {15 Seiten}, year = {2023}, abstract = {In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) synthesized by Medusomyces gisevii have been studied. The culture medium was composed of different initial concentrations of glucose or sucrose dissolved in 0.4\% extract of plain green tea. Parameters of the culture media (titratable acidity, substrate conversion degree etc.) were monitored daily for 20 days of cultivation. The BC pellicles produced on different carbon sources were characterized in terms of biomass yield, crystallinity and morphology by field emission scanning electron microscopy (FE-SEM), atomic force microscopy and X-ray diffraction. Our results showed that Medusomyces gisevii had higher BC yields in media with sugar concentrations close to 10 g L-1 after a 18-20 days incubation period. Glucose in general lead to a higher BC yield (173 g L-1) compared to sucrose (163.5 g L-1). The BC crystallinity degree and surface roughness were higher in the samples synthetized from sucrose. Obtained FE-SEM micrographs show that the BC pellicles synthesized in the sucrose media contained densely packed tangles of cellulose fibrils whereas the BC produced in the glucose media displayed rather linear geometry of the BC fibrils without noticeable aggregates.}, language = {en} } @article{DigelAkimbekovTuralievaetal.2013, author = {Digel, Ilya and Akimbekov, Nuraly S. and Turalieva, M. and Mansurov, Z. and Temiz Artmann, Ayseg{\"u}l and Eshibaev, A. and Zhubanova, A.}, title = {Usage of Carbonized Plant Wastes for Purification of Aqueous Solutions}, series = {Journal of Industrial Technology and Engineering}, volume = {2}, journal = {Journal of Industrial Technology and Engineering}, number = {07}, pages = {47 -- 54}, year = {2013}, language = {en} } @inproceedings{DigelDachwaldArtmannetal.2009, author = {Digel, Ilya and Dachwald, Bernd and Artmann, Gerhard and Linder, Peter and Funke, O.}, title = {A concept of a probe for particle analysis and life detection in icy environments}, year = {2009}, abstract = {A melting probe equipped with autofluorescence-based detection system combined with a light scattering unit, and, optionally, with a microarray chip would be ideally suited to probe icy environments like Europa's ice layer as well as the polar ice layers of Earth and Mars for recent and extinct live.}, subject = {Sonde}, language = {en} }