@article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @article{DelleHuckBaeckeretal.2015, author = {Delle, Lotta E. and Huck, Christina and B{\"a}cker, Matthias and M{\"u}ller, Frank and Grandthyll, Samuel and Jacobs, Karin and Lilischkis, Rainer and Vu, Xuan T. and Sch{\"o}ning, Michael Josef and Wagner, Patrick and Thoelen, Roland and Weil, Maryam and Ingebrandt, Sven}, title = {Impedimetric immunosensor for the detection of histamine based on reduced graphene oxide}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431863}, pages = {1327 -- 1334}, year = {2015}, language = {en} } @article{DeppeBongaertsO'Connelletal.2011, author = {Deppe, Veronika Maria and Bongaerts, Johannes and O'Connell, Timothy and Maurer, Karl-Heinz and Meinhardt, Friedhelm}, title = {Enzymatic deglycation of Amadori products in bacteria}, series = {Applied microbiology and biotechnology}, volume = {Vol. 90}, journal = {Applied microbiology and biotechnology}, number = {Iss. 2}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)}, pages = {399 -- 406}, year = {2011}, language = {en} } @article{DeppeKlatteBongaertsetal.2011, author = {Deppe, Veronika Maria and Klatte, Stephanie and Bongaerts, Johannes and Maurer, Karl-Heinz and O'Connell, Timothy and Meinhardt, Friedhelm}, title = {Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR}, series = {Applied and environmental microbiology}, volume = {Vol. 77}, journal = {Applied and environmental microbiology}, number = {No. 9}, publisher = {American Society of Mechanical Engineers (ASME)}, address = {New York}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, pages = {2839 -- 2846}, year = {2011}, language = {en} } @article{DollWagnerWagneretal.2016, author = {Doll, Theodor and Wagner, Torsten and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Engineering of functional interfaces / Theodor Doll ; Torsten Wagner ; Patrick Wagner ; Michael J. Sch{\"o}ning (eds.)}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201670641}, pages = {1393 -- 1394}, year = {2016}, language = {en} } @article{EmonsBaadeSchoening2000, author = {Emons, H. and Baade, A. and Sch{\"o}ning, Michael Josef}, title = {Voltammetric determination of heavy metals in microvolumes of rain water}, series = {Electroanalysis. 12 (2000), H. 15}, journal = {Electroanalysis. 12 (2000), H. 15}, isbn = {1040-0397}, pages = {1171 -- 1176}, year = {2000}, language = {en} } @article{EmonsGlueckHuellenkremeretal.2001, author = {Emons, H. and Gl{\"u}ck, O. and H{\"u}llenkremer, B. and Sch{\"o}ning, Michael Josef}, title = {Voltohmmetry as an alternative detection method at polycrystalline metal film electrodes}, series = {Electroanalysis. 13 (2001), H. 8-9}, journal = {Electroanalysis. 13 (2001), H. 8-9}, isbn = {1040-0397}, pages = {677 -- 680}, year = {2001}, language = {en} } @article{EmonsGlueckSchoening2001, author = {Emons, H. and Gl{\"u}ck, O. and Sch{\"o}ning, Michael Josef}, title = {Voltohmmetry - An alternative detection principle at ultrathin metal electrodes in solution}, series = {Chemical and biological sensors and analytical methods : proceedings of the international symposium / Sensor, Physical Electrochemistry, and Organic and Biological Electrochemistry Divisions. Ed.: M. Butler}, journal = {Chemical and biological sensors and analytical methods : proceedings of the international symposium / Sensor, Physical Electrochemistry, and Organic and Biological Electrochemistry Divisions. Ed.: M. Butler}, publisher = {Electrochemical Society}, address = {Pennington, NJ}, isbn = {1-56677-351-2}, pages = {1 -- 3}, year = {2001}, language = {en} } @article{EmonsHuellenkremerSchoening2001, author = {Emons, H. and H{\"u}llenkremer, B. and Sch{\"o}ning, Michael Josef}, title = {Detection of metal ions in aqueous solutions by voltohmmetry}, series = {Fresenius' Journal of Analytical Chemistry. 369 (2001), H. 1}, journal = {Fresenius' Journal of Analytical Chemistry. 369 (2001), H. 1}, isbn = {0937-0633}, pages = {42 -- 46}, year = {2001}, language = {en} } @article{EngelmannPourshahidiShalabyetal.2022, author = {Engelmann, Ulrich M. and Pourshahidi, Mohammad Ali and Shalaby, Ahmed and Krause, Hans-Joachim}, title = {Probing particle size dependency of frequency mixing magnetic detection with dynamic relaxation simulation}, series = {Journal of Magnetism and Magnetic Materials}, volume = {563}, journal = {Journal of Magnetism and Magnetic Materials}, number = {In progress, Art. No. 169965}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0304-8853}, doi = {10.1016/j.jmmm.2022.169965}, year = {2022}, abstract = {Biomedical applications of magnetic nanoparticles (MNP) fundamentally rely on the particles' magnetic relaxation as a response to an alternating magnetic field. The magnetic relaxation complexly depends on the interplay of MNP magnetic and physical properties with the applied field parameters. It is commonly accepted that particle core size is a major contributor to signal generation in all the above applications, however, most MNP samples comprise broad distribution spanning nm and more. Therefore, precise knowledge of the exact contribution of individual core sizes to signal generation is desired for optimal MNP design generally for each application. Specifically, we present a magnetic relaxation simulation-driven analysis of experimental frequency mixing magnetic detection (FMMD) for biosensing to quantify the contributions of individual core size fractions towards signal generation. Applying our method to two different experimental MNP systems, we found the most dominant contributions from approx. 20 nm sized particles in the two independent MNP systems. Additional comparison between freely suspended and immobilized MNP also reveals insight in the MNP microstructure, allowing to use FMMD for MNP characterization, as well as to further fine-tune its applicability in biosensing.}, language = {en} } @article{EngelmannSimsekShalabyetal.2024, author = {Engelmann, Ulrich M. and Simsek, Beril and Shalaby, Ahmed and Krause, Hans-Joachim}, title = {Key contributors to signal generation in frequency mixing magnetic detection (FMMD): an in silico study}, series = {Sensors}, volume = {24}, journal = {Sensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s24061945}, pages = {Artikel 1945}, year = {2024}, abstract = {Frequency mixing magnetic detection (FMMD) is a sensitive and selective technique to detect magnetic nanoparticles (MNPs) serving as probes for binding biological targets. Its principle relies on the nonlinear magnetic relaxation dynamics of a particle ensemble interacting with a dual frequency external magnetic field. In order to increase its sensitivity, lower its limit of detection and overall improve its applicability in biosensing, matching combinations of external field parameters and internal particle properties are being sought to advance FMMD. In this study, we systematically probe the aforementioned interaction with coupled N{\´e}el-Brownian dynamic relaxation simulations to examine how key MNP properties as well as applied field parameters affect the frequency mixing signal generation. It is found that the core size of MNPs dominates their nonlinear magnetic response, with the strongest contributions from the largest particles. The drive field amplitude dominates the shape of the field-dependent response, whereas effective anisotropy and hydrodynamic size of the particles only weakly influence the signal generation in FMMD. For tailoring the MNP properties and parameters of the setup towards optimal FMMD signal generation, our findings suggest choosing large particles of core sizes dc > 25 nm nm with narrow size distributions (σ < 0.1) to minimize the required drive field amplitude. This allows potential improvements of FMMD as a stand-alone application, as well as advances in magnetic particle imaging, hyperthermia and magnetic immunoassays.}, language = {en} } @article{ErmelenkoYoshinobuMourzinaetal.2002, author = {Ermelenko, Y. and Yoshinobu, T and Mourzina, Y. and Levichev, S. and Furuichi, K. and Vlasov, Y. and Sch{\"o}ning, Michael Josef and Iwasaki, H.}, title = {Photocurable membranes for ion-selective light-addressable potentiometric sensors}, series = {Sensors and Actuators B. 85 (2002), H. 1-2}, journal = {Sensors and Actuators B. 85 (2002), H. 1-2}, isbn = {0925-4005}, pages = {79 -- 85}, year = {2002}, language = {en} } @article{ErmelenkoYoshinobuMourzinaetal.2001, author = {Ermelenko, Y. and Yoshinobu, T. and Mourzina, Y. and Furuichi, K. and Iwasaki, H. and Vlasov, Y. and Sch{\"o}ning, Michael Josef}, title = {Technology of photocurable polymeric membranes for integrated LAPS}, series = {Proceedings of the 5th East Asian Conference on Chemical Sensors; the 33rd Chemical Sensor Symposium : December 4 - 7, 2001, Huis Ten Bosch, Sasebo-shi, Nagasaki, Japan / Japan Association of Chemical Sensors; the Electrochemical Society of Japan}, journal = {Proceedings of the 5th East Asian Conference on Chemical Sensors; the 33rd Chemical Sensor Symposium : December 4 - 7, 2001, Huis Ten Bosch, Sasebo-shi, Nagasaki, Japan / Japan Association of Chemical Sensors; the Electrochemical Society of Japan}, publisher = {Japan Association of Chemical Sensors}, address = {Kasuga, Fukuoka-ken}, pages = {66 -- 68}, year = {2001}, language = {en} } @article{ErmelenkoYoshinobuMourzinaetal.2002, author = {Ermelenko, Y. and Yoshinobu, T. and Mourzina, Y. and Furuichi, K. and Levichev, S. and Vlasov, Y. and Sch{\"o}ning, Michael Josef and Iwasaki, H.}, title = {Lithium sensor based on the laser scanning semiconductor transducer}, series = {Analytica Chimica Acta. 459 (2002), H. 1}, journal = {Analytica Chimica Acta. 459 (2002), H. 1}, issn = {0378-4304}, pages = {1 -- 9}, year = {2002}, language = {en} } @article{ErmelenkoYoshinobuMourzinaetal.2003, author = {Ermelenko, Y. and Yoshinobu, T. and Mourzina, Y. and Sch{\"o}ning, Michael Josef and Furuichi, K. and Levichev, S. and Vlasov, Y. and Iwasaki, H.}, title = {The double K+/Ca2+ sensor based on laser scanned silicon transducer (LSST) for multicomponent analysis}, series = {Talanta. 59 (2003), H. 4}, journal = {Talanta. 59 (2003), H. 4}, isbn = {0039-9140}, pages = {785 -- 795}, year = {2003}, language = {en} } @article{ErmelenkoYoshinobuMourzinaetal.2002, author = {Ermelenko, Y. and Yoshinobu, T. and Mourzina, Y. and Sch{\"o}ning, Michael Josef and Vlasov, Y. and Iwasaki, H.}, title = {The hybrid K+/Ca2+ sensor based on laser scanned silicon transducer for multi-component analysis}, series = {Proceedings of ICAS 2001, IUPAC [3rd] International Congress on Analytical Sciences 2001 : August 6 - 10, 2001, Waseda University, Tokyo}, journal = {Proceedings of ICAS 2001, IUPAC [3rd] International Congress on Analytical Sciences 2001 : August 6 - 10, 2001, Waseda University, Tokyo}, publisher = {Japan Society for Analytical Chemistry}, address = {Tokyo}, pages = {i777 -- i780}, year = {2002}, language = {en} } @inproceedings{ErmelenkoYoshinobuMourzinaetal.2003, author = {Ermelenko, Y. and Yoshinobu, T. and Mourzina, Y. and Sch{\"o}ning, Michael Josef and Vlasov, Y. and Iwasaki, H.}, title = {A multisensor based on laser scanned silicon transducer (LSST): development and properties}, series = {Eurosensors XVII : the 17th European Conference on Solid-State Transducers ; University of Minho, Guimar{\~a}es, Portugal, September 21 - 24, 2003}, booktitle = {Eurosensors XVII : the 17th European Conference on Solid-State Transducers ; University of Minho, Guimar{\~a}es, Portugal, September 21 - 24, 2003}, pages = {72 -- 73}, year = {2003}, language = {en} } @article{ErmolenkoYoshinobuMourzinaetal.2004, author = {Ermolenko, Y. E. and Yoshinobu, T. and Mourzina, Y. G. and Vlasov, Y. G. and Sch{\"o}ning, Michael Josef and Iwasaki, H.}, title = {Laserscanned transducer (LSST) as a multisensor system}, series = {Sensors and Actuators B. 103 (2004), H. 1-2}, journal = {Sensors and Actuators B. 103 (2004), H. 1-2}, isbn = {0925-4005}, pages = {457 -- 462}, year = {2004}, language = {en} } @article{ErmolenkoVlasovKolodnikovetal.2004, author = {Ermolenko, Y.E. and Vlasov, Y.G. and Kolodnikov, V.V. and Shabaldkin, D. A. and Kloock, Joachim P. and Sch{\"o}ning, Michael Josef}, title = {Diffusion of radioactive tracers (204Tl, 110mAg) and ionic conductivity in membrane materials for the chemical sensors}, series = {Advances in nuclear and radiochemistry : extended abstracts of papers presented at the Sixth International Conference on Nuclear and Radiochemistry (NRC-6), 29 August to 3 September 2004, Aachen, Germany ; in cooperation with University of Cologne, GDCh, FECS, OECD-NEA and IAEA / Syed M. Qaim ... (eds.).}, journal = {Advances in nuclear and radiochemistry : extended abstracts of papers presented at the Sixth International Conference on Nuclear and Radiochemistry (NRC-6), 29 August to 3 September 2004, Aachen, Germany ; in cooperation with University of Cologne, GDCh, FECS, OECD-NEA and IAEA / Syed M. Qaim ... (eds.).}, publisher = {Forschungszentrum J{\"u}lich, Zentralbibliothek}, address = {J{\"u}lich}, isbn = {3893363629}, pages = {483 -- 485}, year = {2004}, language = {en} } @article{FalkenbergBottBongaertsetal.2022, author = {Falkenberg, Fabian and Bott, Michael and Bongaerts, Johannes and Siegert, Petra}, title = {Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae}, series = {Frontiers in Microbiology}, volume = {2022}, journal = {Frontiers in Microbiology}, number = {13}, publisher = {Frontiers}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2022.1017978}, pages = {Artikel 13:1017978}, year = {2022}, abstract = {The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates.}, language = {en} }