@article{AdelsElbersDiehletal.2023,
  author    = {Adels, Klaudia and Elbers, Gereon and Diehl, Bernd and Monakhova, Yulia},
  title     = {Multicomponent analysis of dietary supplements containing glucosamine and chondroitin: comparative low- and high-field NMR spectroscopic study},
  series = {Analytical Sciences},
  volume    = {2023},
  journal   = {Analytical Sciences},
  publisher = {Springer Verlag},
  address   = {Cham},
  issn      = {1348-2246 (Online)},
  doi       = {10.1007/s44211-023-00433-2},
  pages     = {13 Seiten},
  year      = {2023},
  abstract  = {With the prevalence of glucosamine- and chondroitin-containing dietary supplements for people with osteoarthritis in the marketplace, it is important to have an accurate and reproducible analytical method for the quantitation of these compounds in finished products. NMR spectroscopic method based both on low- (80 MHz) and high- (500-600 MHz) field NMR instrumentation was established, compared and validated for the determination of chondroitin sulfate and glucosamine in dietary supplements. The proposed method was applied for analysis of 20 different dietary supplements. In the majority of cases, quantification results obtained on the low-field NMR spectrometer are similar to those obtained with high-field 500-600 MHz NMR devices. Validation results in terms of accuracy, precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit for purpose for the marketed products. The NMR method was extended to the analysis of methylsulfonylmethane, adulterant maltodextrin, acetate and inorganic ions. Low-field NMR can be a quicker and cheaper alternative to more expensive high-field NMR measurements for quality control of the investigated dietary supplements. High-field NMR instrumentation can be more favorable for samples with complex composition due to better resolution, simultaneously giving the possibility of analysis of inorganic species such as potassium and chloride.},
  language  = {en}
}
@misc{BisselsJahn2004,
  author    = {Bissels, Anne and Jahn, Amelie},
  title     = {Multiplex},
  year      = {2004},
  abstract  = {Eine kurze Folge einer Fernsehsendung namens Multiplex. Gesendet wird ein Portait der kubanischen Diva Omara Portuondo auf Europatourn{\´e}e.},
  subject      = {Mediendesign},
  language  = {de}
}
@article{KarschuckSchmidtAchtsnichtetal.2023,
  author    = {Karschuck, Tobias and Schmidt, Stefan and Achtsnicht, Stefan and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef},
  title     = {Multiplexing system for automated characterization of a capacitive field-effect sensor array},
  series = {Physica Status Solidi A},
  volume    = {220},
  journal   = {Physica Status Solidi A},
  number    = {22},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1862-6300 (Print)},
  doi       = {10.1002/pssa.202300265},
  pages     = {7 Seiten},
  year      = {2023},
  abstract  = {In comparison to single-analyte devices, multiplexed systems for a multianalyte detection offer a reduced assay time and sample volume, low cost, and high throughput. Herein, a multiplexing platform for an automated quasi-simultaneous characterization of multiple (up to 16) capacitive field-effect sensors by the capacitive-voltage (C-V) and the constant-capacitance (ConCap) mode is presented. The sensors are mounted in a newly designed multicell arrangement with one common reference electrode and are electrically connected to the impedance analyzer via the base station. A Python script for the automated characterization of the sensors executes the user-defined measurement protocol. The developed multiplexing system is tested for pH measurements and the label-free detection of ligand-stabilized, charged gold nanoparticles.},
  language  = {en}
}
@misc{RoesslerKuehn2004,
  author    = {R{\"o}ßler, Edith and K{\"u}hn, Nicola},
  title     = {Murphy's law},
  year      = {2004},
  abstract  = {Ein Kurzfilm {\"u}ber den letzten Morgen im Leben eines Mannes, das durch Murphy's Law gekennzeichnet ist},
  subject      = {Mediendesign},
  language  = {de}
}
@misc{Tekampe2007,
  author    = {Tekampe, Nina},
  title     = {my own : Spielhaus / Ruhezone f{\"u}r Kinder},
  year      = {2007},
  abstract  = {In der heutigen hektischen und schnelllebigen Zeit ist es auch f{\"u}r Kinder wichtig einen eigenen R{\"u}ckzugsort zu haben, an dem sie ungest{\"o}rt spielen, toben und tr{\"a}umen k{\"o}nnen. Der Entwurf ist eine Spielm{\"o}glichkeit f{\"u}r Kinder, die an der Decke des Kinderzimmers h{\"a}ngt. Dem Kind wird die M{\"o}glichkeit geboten, sich zu verstecken, zu spielen, ein eigenes, geheimes Reich f{\"u}r sich ganz alleine und f{\"u}r Spielkameraden zu haben.},
  subject      = {Kinderm{\"o}bel},
  language  = {de}
}
@masterthesis{Loose2021,
  type      = {Bachelor Thesis},
  author    = {Loose, Pia},
  title     = {Nachhaltige Nutzung textiler Ressourcen: optimiertes Kreislaufsystem f{\"u}r die Wiederverwendung von Textilien},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {179 Seiten},
  year      = {2021},
  abstract  = {Die Fast-Fashion-Industrie produziert am laufenden Band neue Kleidung und so schnell wie sie gekauft wird, wird sie auch wieder entsorgt. Das Altkleidersystem ist allerdings an seiner Kapazit{\"a}tsgrenze angekommen und die sich zuspitzende Lage verlangt nach einer neuen innovativen L{\"o}sung. „VABRIC" ersetzt die herk{\"o}mmlichen Altkleidercontainer in den St{\"a}dten und verl{\"a}ngert den Lebenszyklus von Textilien. Durch eine gezielte Vorsortierung entsteht die M{\"o}glichkeit Kleidung weiterzuverteilen und in bestehende oder ganz neue Nutzungskreisl{\"a}ufe zu integrieren. F{\"u}r die Nutzenden wird die Textilspende durch das hochwertige Erscheinungsbild, die zentralen Standorte und die Aufkl{\"a}rung zu einer positiven Erfahrung. „VABRIC" verk{\"o}rpert, am Beispiel von Textilien, die Vision, wie wir in Zukunft mit vermeintlichem Abfall umgehen und den wahren Wert von Ressourcen hervorheben und nutzbar machen.},
  language  = {de}
}
@masterthesis{Christiani2022,
  type      = {Bachelor Thesis},
  author    = {Christiani, Fabian},
  title     = {Nachhaltiger Bauen : eine interaktive Plattform zum Vergleich von Baustoffen},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {124 Seiten},
  year      = {2022},
  abstract  = {Bei dem Projekt „Nachhaltiger Bauen - Eine interaktive Plattform zum Vergleich von Baustoffen" handelt es sich um eine Datenbank, die verschiedene Baustoffe mit deren Eigenschaften listet und einen Vergleich dieser erm{\"o}glicht. Das Baugewerbe tr{\"a}gt einen erheblichen Teil zum Klimawandel bei. Besonders der CO2-Ausstoß, der Ressourcenverbrauch und die Abfallproduktion sind hier zu nennen. Deshalb ist der Aspekt der Nachhaltigkeit von zentraler Bedeutung f{\"u}r die Zukunft der Baubranche.Ziel des Projekts ist es, sowohl die technischen als auch die {\"o}kologischen Eigenschaften von Baustoffen darzustellen und miteinander zu vergleichen. So kann die Plattform aktiv und effektiv gegen die umwelttechnischen Probleme der Baubranche wirken.},
  language  = {de}
}
@masterthesis{Paulzen2021,
  type      = {Bachelor Thesis},
  author    = {Paulzen, Lena},
  title     = {Nachhaltiges Corporate Packaging Design: Am Beispiel von Naturkosmetik Produkten.},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {124 Seiten},
  year      = {2021},
  abstract  = {Diese Bachelorarbeit widmet sich dem nachhaltigen Packaging-Design von Kosmetikprodukten. Dies wird am Beispiel eines Naturkosmetik-Unternehmens dargestellt. Die Gestaltung einer Shampoo-Flasche wird in dem vorliegenden Projekt exemplarisch f{\"u}r die thematische und gestalterische Untersuchung genutzt. Zudem wurden mit Tube und Tiegel noch zwei weitere Verpackungsformen erstellt. Das Ziel dieser Arbeit ist es, eine nachhaltige Verpackung zu gestalten. Diese soll den hohen CO2-Ausstoß bei der Herstellung von Verpackungen verringern und dabei dem Anspruch des Marketings gerecht werden. Hierbei wurde ein Logo-Design, ein Farbkonzept, eine einheitliche Formsprache und eine Typografie erarbeitet.},
  language  = {de}
}
@inproceedings{SchoeningAbdelghani2012,
  author    = {Sch{\"o}ning, Michael Josef and Abdelghani, Adnane},
  title     = {Nanoscale Science and Technology (NS\&T'12) : Proceedings Book Humboldt Kolleg <2012, Tunisia> ; Tunisia, 17-19 March, 2012 / ed. by Michael J. Sch{\"o}ning ; Adnane Abdelghani},
  url       = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-3544},
  year      = {2012},
  abstract  = {Proceedings of the 2nd Humboldt Kolleg, Hammamet, Tunisia Organizer: Alexander von Humboldt Stiftung, Germany. pdf 184 p. Welcome Address Dear Participants, Welcome to the 2nd Humboldt Kolleg in "Nanoscale Science and Technology" (NS\&T'12) in Tunisia, sponsored by the "Alexander von Humboldt" foundation. The NS\&T'12 multidisciplinary scientific program includes seven "hot" topics dealing with "Nanoscale Science and Technology" covering basic and application-oriented research as well as industrial (market) aspects: - Molecular Biophyics, Spectroscopy Techniques, Imaging Microscopy - Nanomaterials Synthesis for Medicine and Bio-chemical Sensors - Nanostructures, Semiconductors, Photonics and Nanodevices - New Technologies in Market Industry - Environment, Electro-chemistry, Bio-polymers and Fuel Cells - Nanomaterials, Photovoltaic, Modelling, Quantum Physics - Microelectronics, Sensors Networks and Embedded Systems We are deeply indebted to all members of the Scientific Committee and General Chairs for joint Sessions and to all speakers and chairmen, who have dedicated invaluable time and efforts for the realization of this event. On behalf of the Organizing Committee, we are cordially inviting you to join the conference and hope that your stay will be fruitful, rewarding and enjoyable. Prof. Dr. Michael J. Sch{\"o}ning, Prof. Dr. Adnane Abdelghani},
  subject      = {Biosensor},
  language  = {en}
}
@inproceedings{O'HerasDigelTemizArtmann2009,
  author    = {O\'Heras, Carlos and Digel, Ilya and Temiz Artmann, Ayseg{\"u}l},
  title     = {Nanostructured carbon-based column for LPS/protein adsorption : [abstract]},
  year      = {2009},
  abstract  = {The absence of a general method for endotoxin removal from liquid interfaces gives an opportunity to find new methods and materials to overcome this gap. Activated nanostructured carbon is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study is to find the adsorption rates for a carboneous material produced at different temperatures, as well as to reveal possible differences between the performance of the material for each of the adsorbates used during the study (hemoglobin, serum albumin and lipopolysaccharide, LPS).},
  subject      = {Kohlenstofffaser},
  language  = {en}
}
@book{Tuennemann2017,
  author    = {T{\"u}nnemann, Thomas},
  title     = {Neo Forma : Experimente. Workshop Formine 2017},
  publisher = {FH Aachen, Fachbereich Architektur},
  address   = {Aachen},
  isbn      = {978-3-9811003-9-6},
  publisher      = {Fachhochschule Aachen},
  pages     = {92 S. ; zahlr. Ill.},
  year      = {2017},
  abstract  = {Herausgeber: Prof. Dipl.-Ing. Thomas T{\"u}nnemann Katalog Layout; Thilo Haas, Matthias Funken Lektorat: Gerd G{\"o}tschen Titelbild: Kevin Osterkamp Fotos, Yvonne Albers | Fotografin und die jeweiligen Verfasserinnen Impressum | Inhalt 01 Team | Die Besetzung 03 Thomas T{\"u}nnemann | Neo Forma 05 Projekte Carolin Gr{\"u}n | Fare Luce 07 Anke Mannshausen | Tetto Galleggiante 11 Kevin Osterkamp | Azione e Reazione 15 Collin Hackenbroich | Fuori Dentro 21 Lynn Thomas | La Tramoggia 25 Miriam Azzab | l'Incontro 29 Jonas W{\"u}bbe | Le Scale 35 Matthias Funken | Il Laboratorio 39 Moran Dorner | La Via 43 Thilo Haas | Le Scale Vicino 47 Frank Drehsen | Dietro l'Angelo 51 Felix Fischer | La Capella 55 Thomas T{\"u}nnemann | Basilica di Argento 59 Esra Ulutas | Arco Rotondo di Speranza 63 Jana Tillmanns | Punto di Rottura 67 Henri Boh | Il Portale 71 Paul Br{\"u}ggentisch | Silenzio in Movimento 75 Hermann Stuzmann | Scale Antincendio 79 Atmosph{\"a}risches Auf die K{\"u}che | Kulinarisches 81 Carpe Diem | Fotografisches 87},
  language  = {de}
}
@article{BlankeHagenkampDoeringetal.2021,
  author    = {Blanke, Tobias and Hagenkamp, Markus and D{\"o}ring, Bernd and G{\"o}ttsche, Joachim and Reger, Vitali and Kuhnhenne, Markus},
  title     = {Net-exergetic, hydraulic and thermal optimization of coaxial heat exchangers using fixed flow conditions instead of fixed flow rates},
  series = {Geothermal Energy},
  volume    = {9},
  journal   = {Geothermal Energy},
  number    = {Article number: 19},
  publisher = {Springer},
  address   = {Berlin},
  issn      = {2195-9706},
  doi       = {10.1186/s40517-021-00201-3},
  pages     = {23 Seiten},
  year      = {2021},
  abstract  = {Previous studies optimized the dimensions of coaxial heat exchangers using constant mass fow rates as a boundary condition. They show a thermal optimal circular ring width of nearly zero. Hydraulically optimal is an inner to outer pipe radius ratio of 0.65 for turbulent and 0.68 for laminar fow types. In contrast, in this study, fow conditions in the circular ring are kept constant (a set of fxed Reynolds numbers) during optimization. This approach ensures fxed fow conditions and prevents inappropriately high or low mass fow rates. The optimization is carried out for three objectives: Maximum energy gain, minimum hydraulic efort and eventually optimum net-exergy balance. The optimization changes the inner pipe radius and mass fow rate but not the Reynolds number of the circular ring. The thermal calculations base on Hellstr{\"o}m's borehole resistance and the hydraulic optimization on individually calculated linear loss of head coefcients. Increasing the inner pipe radius results in decreased hydraulic losses in the inner pipe but increased losses in the circular ring. The net-exergy diference is a key performance indicator and combines thermal and hydraulic calculations. It is the difference between thermal exergy fux and hydraulic efort. The Reynolds number in the circular ring is instead of the mass fow rate constant during all optimizations. The result from a thermal perspective is an optimal width of the circular ring of nearly zero. The hydraulically optimal inner pipe radius is 54\% of the outer pipe radius for laminar fow and 60\% for turbulent fow scenarios. Net-exergetic optimization shows a predominant infuence of hydraulic losses, especially for small temperature gains. The exact result depends on the earth's thermal properties and the fow type. Conclusively, coaxial geothermal probes' design should focus on the hydraulic optimum and take the thermal optimum as a secondary criterion due to the dominating hydraulics.},
  language  = {en}
}
@article{PicardBrillCasseletal.2002,
  author    = {Picard, Antoni and Brill, Manfred and Cassel, Detlev and Jentsch, Andreas and Rollwa, Markus and K{\"a}mper, Klaus-Peter and Merten, Sabine},
  title     = {Neue Medien f{\"u}r die praktische MST-Ausbildung},
  organization    = {FH-Kaiserslautern},
  year      = {2002},
  abstract  = {Im Studiengang Mikrosystemtechnik des Fachhochschulstandortes Zweibr{\"u}cken werden zwei neue moderne Anlagen f{\"u}r die Herstellung von mikrotechnischen Komponenten in Betrieb genommen: Ein Oxidationsofen f{\"u}r Herstellung d{\"u}nner Oxidschichten auf Silizium-Einkristallen und eine Belichtungsapparatur f{\"u}r die Fotolithografie - das Besondere an diesen Anlagen: Sie existieren nur virtuell, d.h. als Animationen in einer Computerwelt.},
  subject      = {Mikrosystemtechnik},
  language  = {de}
}
@inproceedings{KernMeppelink2001,
  author    = {Kern, Alexander and Meppelink, Jan},
  title     = {Neue M{\"o}glichkeiten elektrischer Anschl{\"u}sse an die Bewehrung und Untersuchung der Wirkung von Blitzstr{\"o}men in bewehrtem Beton},
  year      = {2001},
  abstract  = {Im Rahmen eines modernen Blitzschutzsystems f{\"u}r Stahlbeton-Bauten bietet es sich an, die Betonbewehrung zu benutzen: - Sie kann die Funktionen der Ableitungseinrichtungen und des Blitzschutz- Potentialausgleichs bei einem klassischen Geb{\"a}ude-Blitzschutz {\"u}bernehmen [1]; - Sie kann, ggf. bei entsprechender Erg{\"a}nzung, als ein geschlossener K{\"a}fig ausgebildet werden und damit eine deutliche Reduzierung der Belastung elektrischer / elektronischer Systeme durch blitzinduzierte elektromagnetische Felder erbringen (LEMP-Schutz [2]). Die Nutzung der Bewehrung ist dabei grunds{\"a}tzlich gleichermaßen bei Neubauten wie auch bei Ert{\"u}chtigungen m{\"o}glich und sinnvoll. So stellt die Nutzung der Bewehrung beispielsweise im Bereich von Großkraftwerken eine wesentliche Ert{\"u}chtigungsmaßnahme f{\"u}r den Blitzschutz elektrischer und elektronischer Einrichtungen dar: - Einerseits wird der Blitzschutz-Potentialausgleich durch den Anschluss metallener Einrichtungen wie Elektronik-Schr{\"a}nke, Kabeltrag-Konstruktionen, Rohrleitungen, etc. an die Bewehrung deutlich verbessert. - Andererseits kann bei gr{\"o}ßeren Geb{\"a}uden die elektromagnetische Schirmwirkung durch die elektrische {\"U}berbr{\"u}ckung von vorhandenen Dehnfugen bei Stahlbetonbauten optimiert werden. Diese Dehnfugen sind teilweise nur unzureichend {\"u}berbr{\"u}ckt, so dass bei Blitzeinschlag in das betreffende oder ein benachbartes Geb{\"a}ude an Kabelstrecken, die {\"u}ber die Dehnfuge hinwegf{\"u}hren, rel. hohe Spannungen induziert werden k{\"o}nnen [2, 3]. Die sich um das gesamte Geb{\"a}ude herumziehende oder zwischen zwei Geb{\"a}uden befindliche Dehnfuge muss deshalb im Abstand von maximal einigen Metern {\"u}berbr{\"u}ckt werden. Im Falle von Blitzschutz-Ert{\"u}chtigungen in vorhandenen Geb{\"a}uden wird bisher an jeder geplanten Anschlussstelle die Bewehrung großfl{\"a}chig (\&\#8709; wenige 10 cm) freigelegt, dort ein elektrischer Anschluss zu dem Bewehrungsstab hergestellt, z.B. mittels eines Erdungsfestpunkts, und dann die Betonoberfl{\"a}che wieder geschlossen. Je nach prognostizierter Strombelastung wird teilweise versucht, den {\"u}ber den Anschluss fließenden Strom bereits auf mehrere Bewehrungsst{\"a}be zu verteilen. Dazu sind entweder die kreuzenden St{\"a}be zu verschweißen oder es sind direkt Anschl{\"u}sse an zwei Bewehrungsst{\"a}be herzustellen. All dieses bedeutet einen hohen Aufwand bei der Freilegung der Bewehrung und auch wieder bei der Schließung der entstandenen Betonl{\"o}cher. Es soll deshalb hier untersucht werden, ob es beispielsweise zum Zwecke des Blitzschutz-Potentialausgleichs und auch zur {\"U}berbr{\"u}ckung von Dehnfugen ausreichend ist, den Anschluss an die Bewehrung nach einfachen Verfahren nur jeweils an einen Bewehrungsstab herzustellen. Damit w{\"u}rde der finanzielle und administrative Aufwand an Betonarbeiten deutlich reduziert. Die hier dargestellten Verfahren sind dabei insbesondere f{\"u}r den Einsatz bei Blitzschutz-Ert{\"u}chtigungen in bestehenden Geb{\"a}uden vorgesehen. Abschließend sollen deshalb die M{\"o}glichkeiten zur Pr{\"u}fung korrekter Anschl{\"u}sse, die Grenzen der Verfahren sowie auch die Grenzen der Anwendbarkeit bei Neuanlagen diskutiert werden.},
  language  = {de}
}
@article{FalkenbergVossBottetal.2023,
  author    = {Falkenberg, Fabian and Voß, Leonie and Bott, Michael and Bongaerts, Johannes and Siegert, Petra},
  title     = {New robust subtilisins from halotolerant and halophilic Bacillaceae},
  series = {Applied Microbiology and Biotechnology},
  volume    = {107},
  journal   = {Applied Microbiology and Biotechnology},
  publisher = {Springer Nature},
  address   = {Berlin},
  issn      = {1432-0614},
  doi       = {10.1007/s00253-023-12553-w},
  pages     = {3939 -- 3954},
  year      = {2023},
  abstract  = {The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0-12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5\% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications.},
  language  = {en}
}
@inproceedings{GellertNordaButenweg2008,
  author    = {Gellert, Christoph and Norda, Hannah and Butenweg, Christoph},
  title     = {Nonlinear behaviour of masonry under cyclic loading},
  series = {7th European Conference on Structural Dynamics, EURODYN 2008 : 7-9 July 2008, Southampton},
  booktitle = {7th European Conference on Structural Dynamics, EURODYN 2008 : 7-9 July 2008, Southampton},
  organization    = {European Conference on Structural Dynamics, EURODYN <7, 2008, Southampton>},
  pages     = {1 -- 12},
  year      = {2008},
  language  = {en}
}
@article{HaegerProbstJaegeretal.2023,
  author    = {Haeger, Gerrit and Probst, Johanna and Jaeger, Karl-Erich and Bongaerts, Johannes and Siegert, Petra},
  title     = {Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans},
  series = {FEBS Open Bio},
  volume    = {13},
  journal   = {FEBS Open Bio},
  number    = {12},
  publisher = {Wiley},
  address   = {Hoboken, NJ},
  issn      = {2211-5463},
  doi       = {10.1002/2211-5463.13723},
  pages     = {2224 -- 2238},
  year      = {2023},
  abstract  = {Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.},
  language  = {en}
}
@inproceedings{AridaKloockSchoening2006,
  author    = {Arida, Hassan A. and Kloock, Joachim P. and Sch{\"o}ning, Michael Josef},
  title     = {Novel organic membrane-based thin-film microsensors for the determination of heavy metal cations},
  url       = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-1545},
  year      = {2006},
  abstract  = {A first step towards the fabrication and electrochemical evaluation of thin-film microsensors based on organic PVC membranes for the determination of Hg(II), Cd(II), Pb(II) and Cu(II) ions in solutions has been realised. The membrane-coating mixture used in the preparation of this new type of microsensors is incorporating PVC as supporting matrix, o-nitrophenyloctylether (o-NPOE) as solvent mediator and a recently synthesized Hg[dimethylglyoxime(phene)]2+ and Bis-(4-hydroxyacetophenone)-ethylenediamine as electroactive materials for Hg(II) and Cd(II), respectively. A set of three commercialised ionophores for Cd(II), Pb(II) and Cu(II) has been also used for comparison. Thin-film microsensors based on these membranes showed a Nernstian response of slope (26-30 mV/dec.) for the respective tested cations. The potentiometric response characteristics (linear range, pH range, detection limit and response time) are comparable with those obtained by conventional membranes as well as coated wire electrodes prepared from the same membrane. The realisation of the new organic membrane-based thin-film microsensors overcomes the problem of an insufficient selectivity of solid-state-based thinfilm sensors.},
  subject      = {Biosensor},
  language  = {en}
}
@article{HaegerJolmesOyenetal.2024,
  author    = {Haeger, Gerrit and Jolmes, Tristan and Oyen, Sven and Jaeger, Karl-Erich and Bongaerts, Johannes and Sch{\"o}rken, Ulrich and Siegert, Petra},
  title     = {Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis},
  series = {Applied Microbiology and Biotechnology},
  journal   = {Applied Microbiology and Biotechnology},
  number    = {108},
  publisher = {Springer},
  address   = {Berlin},
  issn      = {1432-0614},
  doi       = {10.1007/s00253-023-12868-8},
  pages     = {14 Seiten},
  year      = {2024},
  abstract  = {N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75\%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC-MS and NMR.},
  language  = {en}
}
@techreport{Wessling2006,
  type      = {Working Paper},
  author    = {Weßling, Matthias},
  title     = {O Perfil Reiss: Conceito scientifico e conclus{\~o}es da pesquisa, aplica{\c{c}}{\~o}es exemplaricas e conseq{\"u}{\^e}ncias pr{\´a}ticas},
  year      = {2006},
  abstract  = {Para Reiss, a avalia{\c{c}}{\~a}o de cada um dos 16 motivos vitais {\´e} a chave para n{\~a}o s{\´o} entender o comportamento humano, como tamb{\´e}m para poder prev{\^e}-lo. Tal previs{\~a}o {\´e} tamb{\´e}m a meta de cada ci{\^e}ncia. Quando se quer saber a rea{\c{c}}{\~a}o das pessoas, precisa-se primeiro descobrir, o que as pessoas realmente querem - e, ent{\~a}o prever, que elas satisfar{\~a}o esses desejos e necessidades em suas a{\c{c}}{\~o}es. As chances, que o Perfil Reiss oferece, resultam entre outras tamb{\´e}m, que aqui {\´e} poss{\´i}vel pela primeira vez uma individualiza{\c{c}}{\~a}o total. Cerca de 6 bilh{\~o}es de perfis individuais podem ser apresentados com o Perfil Reiss. Na combina{\c{c}}{\~a}o correta da forma{\c{c}}{\~a}o e utiliza{\c{c}}{\~a}o da tipologia de um lado e da individualidade da conclus{\~a}o de outro lado, est{\´a} a for{\c{c}}a positiva desse instrumento.},
  subject      = {Motivation},
  language  = {pt}
}