@article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{FerreinNiemuellerSteinbauer2010, author = {Ferrein, Alexander and Niem{\"u}ller, Tim and Steinbauer, Gerald}, title = {Team Zadeat 2010 : application for participation}, pages = {5 Seiten}, year = {2010}, language = {en} } @book{Thomas2010, author = {Thomas, Axel}, title = {Rhetorische Knallfr{\"o}sche : Zitatensammlung f{\"u}r Politik und Wirtschaft}, publisher = {Neck + Heyn}, address = {Aachen}, isbn = {978-3-00-033037-7}, pages = {145 S. : Ill.}, year = {2010}, language = {de} } @inproceedings{WalterGligorevicDetertetal.2010, author = {Walter, Michael and Gligorevic, Snjezana and Detert, Thorben and Schnell, Michael}, title = {UHF/VHF air-to-air propagation measurements}, series = {Proceedings of the Fourth European Conference on Antennas and Propagation (EuCAP) : 12 - 16 April 2010, Barcelona, Spain}, booktitle = {Proceedings of the Fourth European Conference on Antennas and Propagation (EuCAP) : 12 - 16 April 2010, Barcelona, Spain}, organization = {European Association on Antennas and Propagation}, isbn = {978-1-4244-6431-9 ; 978-84-7653-472-4}, pages = {1 -- 5}, year = {2010}, language = {en} } @inproceedings{SchnellFranzenGligorevic2010, author = {Schnell, Michael and Franzen, Nico and Gligorevic, Snjezana}, title = {L-DACS1 laboratory demonstrator development and compatibility measurement set-up}, series = {IEEE/AIAA 29th Digital Avionics Systems Conference (DASC) : 3 - 7 Oct. 2010, Salt Lake City, Utah}, booktitle = {IEEE/AIAA 29th Digital Avionics Systems Conference (DASC) : 3 - 7 Oct. 2010, Salt Lake City, Utah}, organization = {Institute of Electrical and Electronics Engineers}, isbn = {9781424466160 ; 9781424466184}, pages = {3E3-1 -- 3E3-11}, year = {2010}, language = {en} } @inproceedings{GligorevicPulini2010, author = {Gligorevic, Snjezana and Pulini, Paola}, title = {Simplified airport surface channel model based on the WSSUS assumption}, series = {2010 Integrated Communications, Navigation and Surveillance Conference (ICNS 2010) : Herndon, Virginia, USA, 11 - 13 May 2010}, booktitle = {2010 Integrated Communications, Navigation and Surveillance Conference (ICNS 2010) : Herndon, Virginia, USA, 11 - 13 May 2010}, organization = {Institute of Electrical and Electronics Engineers}, isbn = {978-1-4244-7457-8}, issn = {2155-4943}, pages = {F2-1 -- F2-11}, year = {2010}, language = {en} } @misc{Alt2010, author = {Alt, Helmut}, title = {Optionen einer bezahlbaren Energieversorgung „Vision versus Realismus" : Beitrag f{\"u}r KTG Kongress: vom 4. bis 6. Mai 2010, Berlin}, pages = {1 -- 10}, year = {2010}, language = {de} } @misc{Alt2010, author = {Alt, Helmut}, title = {Vom Studium der Kerntechnik zu Energie zwischen Wunsch und Wirklichkeit : [Vortrag : Verleihung der KTG-Ehrenmitgliedschaft, Berlin, November 2010]}, pages = {1 -- 15}, year = {2010}, language = {de} } @book{BuyerKleinMueller2010, author = {Buyer, Christoph and Klein, Hartmut and M{\"u}ller, Thomas}, title = {{\"A}nderung der Unternehmensform : Handbuch zum Umwandlungs- und Umwandlungssteuerrecht}, edition = {8. Aufl.}, publisher = {Verl. Neue Wirtschafts-Briefe}, address = {Herne}, isbn = {978-3-482-42258-4}, pages = {XL, 585 S.}, year = {2010}, language = {de} } @inproceedings{FeldhusenBrezingPuetzetal.2010, author = {Feldhusen, J{\"o}rg and Brezing, Alexander Nikolaus and P{\"u}tz, Claus and W{\"a}hlisch, Georg}, title = {Multi-system CAD-teaching in large classes}, series = {When design education and design research meet : proceedings of the 12th International Conference on Engineering and Product Design Education, Norwegian University of Science and Technology (NTNU) Trondheim, Norway, 2nd - 3rd September 2010 ; [E\&PDE]}, booktitle = {When design education and design research meet : proceedings of the 12th International Conference on Engineering and Product Design Education, Norwegian University of Science and Technology (NTNU) Trondheim, Norway, 2nd - 3rd September 2010 ; [E\&PDE]}, publisher = {Design Society}, address = {Glasgow}, organization = {International Conference on Engineering and Product Design Education <12, 2010, Trondheim>}, isbn = {978-1-904670-19-3}, pages = {204 -- 209}, year = {2010}, language = {en} } @inproceedings{Bung2010, author = {Bung, Daniel Bernhard}, title = {A comparative study of self-aerated stepped spillway and smooth invert chute flow: the effect of step-induced macro roughness}, series = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, booktitle = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, publisher = {Univ. Press}, address = {Tianjin}, organization = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <5, 2010, Tianjin>}, isbn = {978-7-5618-3671-2}, pages = {451 -- 456}, year = {2010}, language = {en} } @inproceedings{KerpenBungSchlurmann2010, author = {Kerpen, N. B. and Bung, Daniel Bernhard and Schlurmann, Torsten}, title = {Physical model investigations of pressure distributions next to ships passing through a lock}, series = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, booktitle = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, publisher = {Univ. Press}, address = {Tianjin}, organization = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <5, 2010, Tianjin>}, isbn = {978-7-5618-3671-2}, pages = {514 -- 519}, year = {2010}, language = {en} } @inproceedings{BungSchlenkhoff2010, author = {Bung, Daniel Bernhard and Schlenkhoff, Andreas}, title = {Self-aerated skimming flow on embankment stepped spillways : the effect of additional micro-roughness on energy dissipation and oxygen transfer}, series = {Proceedings from first IAHR European congress : May 2010, Edinburgh}, booktitle = {Proceedings from first IAHR European congress : May 2010, Edinburgh}, publisher = {Heriot-Watt University, School of the Built Environment}, address = {Edinburgh}, organization = {International Association for Hydraulic Engineering and Research}, isbn = {9780956595102}, pages = {Artikelkennnummer HSIIId}, year = {2010}, language = {en} } @inproceedings{KerpenBungSchlurmann2010, author = {Kerpen, N. B. and Bung, Daniel Bernhard and Schlurmann, Torsten}, title = {Physical model investigations of ships passing through a lock}, series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, editor = {Janssen, Robert}, publisher = {School of Civil Engineeering, The University of Queensland}, address = {Brisbane}, organization = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>}, isbn = {9781742720159}, pages = {93 -- 100}, year = {2010}, language = {en} } @inproceedings{HeinzOertelBung2010, author = {Heinz, G. and Oertel, Mario and Bung, Daniel Bernhard}, title = {Investigations on a cooling water system for a gas tubine facility concerning air vortexes and sediment transport}, series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, editor = {Janssen, Robert}, publisher = {School of Civil Engineering, The University of Queensland}, address = {Brisbane}, organization = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>}, isbn = {9781742720159}, pages = {109 -- 116}, year = {2010}, language = {en} } @inproceedings{BungSchlenkhoff2010, author = {Bung, Daniel Bernhard and Schlenkhoff, Andreas}, title = {Selbstbel{\"u}ftete Gerinnestr{\"o}mungen auf Treppenschussrinnen}, series = {Talsperren im Wandel : 15. Deutsches Talsperrensymposium ; Beitr{\"a}ge zur Tagung am 14. bis 16. April 2010 im Eurogress Aachen}, booktitle = {Talsperren im Wandel : 15. Deutsches Talsperrensymposium ; Beitr{\"a}ge zur Tagung am 14. bis 16. April 2010 im Eurogress Aachen}, organization = {Deutsches TalsperrenKomitee}, isbn = {978-3-8322-9043-6}, pages = {39 -- 47}, year = {2010}, language = {de} } @inproceedings{BungOertelSchlenkhoffetal.2010, author = {Bung, Daniel Bernhard and Oertel, M. and Schlenkhoff, A. and Schlurmann, Torsten}, title = {Flash flood awareness and prevention in Germany}, series = {Early warning for flash floods : international workshop, Praha 2011 : workshop proceedings}, booktitle = {Early warning for flash floods : international workshop, Praha 2011 : workshop proceedings}, editor = {Obrusnik, Ivan}, organization = {Czech Hydrometeorological Institute}, isbn = {978-80-86690-91-9}, pages = {34 -- 40}, year = {2010}, language = {en} } @article{KuhnhenneDoeringFeldmann2010, author = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Feldmann, Markus}, title = {Grunds{\"a}tze und L{\"o}sungen zur W{\"a}rmebr{\"u}ckenreduktion im Metallleichtbau}, series = {Stahlbau}, volume = {79}, journal = {Stahlbau}, number = {5}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1437-1049 (E-Journal); 0038-9145 (Print)}, doi = {10.1002/stab.201001330}, pages = {345 -- 355}, year = {2010}, abstract = {Die Anforderungen an das energiesparende Bauen sind mit der Einf{\"u}hrung der Energieeinsparverordnung (EnEV) 2009 auch im Industrie- und Gewerbebau deutlich versch{\"a}rft worden. Einen wesentlichen Beitrag zur Energieeinsparung liefert die Minimierung des Transmissionsw{\"a}rmetransfers. Analysiert man Geb{\"a}udeh{\"u}llen in Metallleichtbauweise stellt man fest, dass eine Erh{\"o}hung der W{\"a}rmed{\"a}mmst{\"a}rke allein noch nicht zielf{\"u}hrend ist, zus{\"a}tzlich sind W{\"a}rmebr{\"u}ckeneffekte zu ber{\"u}cksichtigen und deren Einfl{\"u}sse auf die W{\"a}rmetransmission zu reduzieren. Neben der Bedeutung f{\"u}r die Energieeinsparung ist eine w{\"a}rmetechnisch optimierte Detailausbildung auch erforderlich, um einen ausreichenden Feuchteschutz (Vermeidung von Tauwasser und Schimmelpilz) zu realisieren und so Sch{\"a}den zu vermeiden. Ein wichtiges Hilfsmittel stellt hierzu der vom Industrieverband f{\"u}r Bausysteme im Metallleichtbau (IFBS) herausgegebene W{\"a}rmebr{\"u}ckenatlas der Metall-Sandwichbauweise dar.}, language = {de} } @article{KuhnhenneDoeringKockeretal.2010, author = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Kocker, Ronald and Pyschny, Dominik and Feldmann, Markus}, title = {Die {\"O}kobilanz als Baustein der Nachhaltigkeitsbewertung im Industrie- und Gewerbebau}, series = {Stahlbau}, volume = {79}, journal = {Stahlbau}, number = {6}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1437-1049 (E-Journal); 0038-9145 (Print)}, doi = {10.1002/stab.201001336}, pages = {439 -- 447}, year = {2010}, abstract = {Im Herbst 2009 wurde das "Deutsche G{\"u}tesiegel Nachhaltiges Bauen" f{\"u}r die Geb{\"a}udetypen Industriebauten und Handelsbauten offiziell vorgestellt. Die {\"O}kobilanz als wichtiger Baustein der Nachhaltigkeitsbewertung wird somit auch im Industrie- und Gewerbebau zuk{\"u}nftig stark an Bedeutung gewinnen. Der vorliegende Beitrag beinhaltet einen Vergleich der Umweltleistung verschiedener Bauweisen des Industrie- und Gewerbebaus, die durch die {\"O}kobilanzierung quantifizierbar wird, anhand von momentan verf{\"u}gbaren Daten und Methoden. Es werden ausgew{\"a}hlte Ergebnisse sowie vorhandene Unsicherheiten und daraus resultierender Forschungsbedarf aufgezeigt.}, language = {de} } @incollection{AbelnDoeringFeldmannetal.2010, author = {Abeln, Bj{\"o}rn and D{\"o}ring, Bernd and Feldmann, Markus and Geßler, Achim and M{\"o}ller, Sebastian and Paschen, Michael and Sedlacek, Gerhard}, title = {Entwicklungen zu Stahlfahrbahnen von Straßenbr{\"u}cken}, series = {Festschrift zum 60. Geburtstag von Univ.-Prof. Dr.-Ing. Ingbert Mangerig. (Berichte aus dem Konstruktiven Ingenieurbau / Universit{\"a}t der Bundeswehr M{\"u}nchen ; 10/6)}, booktitle = {Festschrift zum 60. Geburtstag von Univ.-Prof. Dr.-Ing. Ingbert Mangerig. (Berichte aus dem Konstruktiven Ingenieurbau / Universit{\"a}t der Bundeswehr M{\"u}nchen ; 10/6)}, publisher = {Univ. der Bundeswehr}, address = {Neubiberg}, pages = {65 -- 76}, year = {2010}, language = {de} }