@article{KirchnerSpelthahnSchoeningetal.2010, author = {Kirchner, Patrick and Spelthahn, H. and Sch{\"o}ning, Michael Josef and Henkel, H. and Schneider, A. and Friedrich, P. and Kolstad, J. and Berger, J.}, title = {Realisierung eines Polyimid-basierten kalorimetrischen Gassensors zur Inline-{\"U}berwachung der H2O2-Konzentration in aseptischen Abf{\"u}llanlagen}, series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, journal = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {607 -- 612}, year = {2010}, language = {de} } @article{WernerSpelthahnSchoeningetal.2010, author = {Werner, Frederik and Spelthahn, H. and Sch{\"o}ning, Michael Josef and Krumbe, C. and Wagner, Torsten and Yoshinobu, T. and Keusgen, M.}, title = {Neue Ansteuerungselektronik f{\"u}r LAPS-basierte Biosensoren zur gleichzeitig ortsaufgel{\"o}sten Messung der pH-Konzentration}, series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, journal = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {109 -- 114}, year = {2010}, language = {de} } @article{PoghossianWagnerSchoening2010, author = {Poghossian, Arshak and Wagner, H. and Sch{\"o}ning, Michael Josef}, title = {Automatisiertes „wafer level"-Testsystem zur Charakterisierung von siliziumbasierten Chemo- und Biosensoren}, series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, journal = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {89 -- 92}, year = {2010}, language = {de} } @article{WagnerMiyamotoSchoeningetal.2010, author = {Wagner, Torsten and Miyamoto, Ko-ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Novel combination of digital light processing (DLP) and light-addressable potentiometric sensors (LAPS) for flexible chemical imaging}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {520 -- 523}, year = {2010}, language = {en} } @article{KirchnerOberlaenderFriedrichetal.2010, author = {Kirchner, Patrick and Oberl{\"a}nder, Jan and Friedrich, Peter and Rysstad, G. and Berger, J. and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Realization of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {264 -- 267}, year = {2010}, language = {en} } @article{MiyamotoKanekoMatsuoetal.2010, author = {Miyamoto, Ko-ichiro and Kaneko, Kazumi and Matsuo, Akira and Wagner, Torsten and Kanoh, Shin`ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Miniaturized chemical imaging sensor system using an OLED display panel}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {516 -- 519}, year = {2010}, language = {en} } @article{BohrnStuetzFleischeretal.2010, author = {Bohrn, U. and St{\"u}tz, E. and Fleischer, M. and Sch{\"o}ning, Michael Josef}, title = {Real-time detection of CO by eukaryotic cells}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {17 -- 20}, year = {2010}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @inproceedings{BegingPoghossianMlyneketal.2010, author = {Beging, Stefan and Poghossian, Arshak and Mlynek, D. and Hataihimakul, S. and Pedraza, A. and Dhawan, S. and Laube, N. and Kleinen, L. and Baldsiefen, G. and Busch, H. and Sch{\"o}ning, Michael Josef}, title = {Ion-selective sensors for the determination of the risk of urinary stone formation}, series = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, booktitle = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, address = {Warsaw}, pages = {74 -- 80}, year = {2010}, language = {en} } @article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @article{SchoeningAbouzarPoghossian2009, author = {Sch{\"o}ning, Michael Josef and Abouzar, Maryam H. and Poghossian, Arshak}, title = {pH and ion sensitivity of a field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with polyelectrolyte multilayers}, series = {Journal of Solid State Electrochemistry. 13 (2009), H. 1}, journal = {Journal of Solid State Electrochemistry. 13 (2009), H. 1}, isbn = {1433-0768}, pages = {115 -- 122}, year = {2009}, language = {en} } @article{SchoeningSchuetz2009, author = {Sch{\"o}ning, Michael Josef and Sch{\"u}tz, S.}, title = {Der K{\"a}fer/Chip-Sensor als bioelektronischer "Schn{\"u}ffler"}, series = {Bioanalytik f{\"u}r Einsteiger : [Diabetes, Drogen und DNA] / Reinhard Renneberg. Darja S{\"u}ßbier (Ill.). [Red.: Andreas Held ...]}, journal = {Bioanalytik f{\"u}r Einsteiger : [Diabetes, Drogen und DNA] / Reinhard Renneberg. Darja S{\"u}ßbier (Ill.). [Red.: Andreas Held ...]}, publisher = {Spektrum, Akad. Verl.}, address = {Heidelberg}, isbn = {978-3-8274-1831-9}, pages = {204 -- 205}, year = {2009}, language = {de} } @article{AbouzarPoghossianRazavietal.2009, author = {Abouzar, Maryam H. and Poghossian, Arshak and Razavi, A. and Williams, O. A. and Bijnens, N. and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Characterisation of capacitive field-effect sensors with a nanocrystalline-diamond film as transducer material for multi-parameter sensing}, series = {Biosensors and Bioelectronics. 24 (2009), H. 5}, journal = {Biosensors and Bioelectronics. 24 (2009), H. 5}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0956-5663}, pages = {1298 -- 1304}, year = {2009}, language = {en} } @article{PoghossianIngebrandtOffenhaeusseretal.2009, author = {Poghossian, Arshak and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Field-effect devices for detecting cellular signals}, series = {Seminars in Cell \& Developmental Biology. 20 (2009), H. 1}, journal = {Seminars in Cell \& Developmental Biology. 20 (2009), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1096-3634}, pages = {41 -- 48}, year = {2009}, language = {en} } @article{AridaMohsenSchoening2009, author = {Arida, Hassan and Mohsen, Q. and Sch{\"o}ning, Michael Josef}, title = {Microfabrication, characterization and analytical application of a new thin-film silver microsensor}, series = {Electrochimica Acta. 54 (2009), H. 13}, journal = {Electrochimica Acta. 54 (2009), H. 13}, isbn = {0013-4686}, pages = {3543 -- 3547}, year = {2009}, language = {en} } @article{KraemerPitaZhouetal.2009, author = {Kr{\"a}mer, Melina and Pita, Marcos and Zhou, Jian and Ornatska, Maryna and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Katz, Evgeny}, title = {Coupling of Biocomputing Systems with Electronic Chips: Electronic Interface for Transduction of Biochemical Information}, series = {Journal of Physical Chemistry C: Nanomaterials and Interfaces. 113 (2009), H. 6}, journal = {Journal of Physical Chemistry C: Nanomaterials and Interfaces. 113 (2009), H. 6}, publisher = {American Cemical Society}, address = {Washington, DC}, isbn = {1932-7455}, pages = {2573 -- 2579}, year = {2009}, language = {en} } @article{SiqueiraWernerBaeckeretal.2009, author = {Siqueira, Jose R. and Werner, Frederik and B{\"a}cker, Matthias and Poghossian, Arshak and Zucolotto, Valtencir and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Layer-by-Layer Assembly of Carbon Nanotubes Incorporated in Light-Addressable Potentiometric Sensors}, series = {Journal of Physical Chemistry C. 113 (2009), H. 33}, journal = {Journal of Physical Chemistry C. 113 (2009), H. 33}, publisher = {American Chemical Society}, address = {Washington, DC}, isbn = {1932-7455}, pages = {14765 -- 14770}, year = {2009}, language = {en} } @article{AridaTurekRolkaetal.2009, author = {Arida, Hassan and Turek, Monika and Rolka, David and Sch{\"o}ning, Michael Josef}, title = {A Novel Thin-Film Copper Array Based on an Organic/Inorganic Sensor Hybrid: Microfabrication, Potentiometric Characterization, and Flow-Injection Analysis Application}, series = {Electroanalysis. 21 (2009), H. 10}, journal = {Electroanalysis. 21 (2009), H. 10}, publisher = {Wiley}, address = {Weinheim}, isbn = {1040-0397}, pages = {1145 -- 1151}, year = {2009}, language = {en} } @article{MiyamotoKuwabaraKanohetal.2009, author = {Miyamoto, K. and Kuwabara, Yohei and Kanoh, Shin'ichiro and Yoshinobu, Tatsuo and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Chemical image scanner based on FDM-LAPS}, series = {Sensors and Actuators B: Chemical. 137 (2009), H. 2}, journal = {Sensors and Actuators B: Chemical. 137 (2009), H. 2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0925-4005}, pages = {533 -- 538}, year = {2009}, language = {en} } @article{MorenoiCodinachsBirkenstockGarmaetal.2009, author = {Moreno i Codinachs, L. and Birkenstock, C. and Garma, T. and Zierold, R. and Bachmann, J. and Nielsch, K. and Sch{\"o}ning, Michael Josef and Fontcuberta i Morral, A.}, title = {A micron-sized nanoporous multifunction sensing device}, series = {physica status solidi (a) . 206 (2009), H. 3}, journal = {physica status solidi (a) . 206 (2009), H. 3}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {435 -- 441}, year = {2009}, language = {en} }