@article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @inproceedings{GoldbachAment2010, author = {Goldbach, Daniel and Ament, Christoph}, title = {Stabilized reversing with vehicle trailer combinations}, series = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau}, booktitle = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau}, editor = {Scharff, Peter}, publisher = {Verl. ISLE}, address = {Ilmenau}, organization = {Technische Universit{\"a}t / Fakult{\"a}t f{\"u}r Informatik und Automatisierung}, isbn = {978-3-938843-53-6}, pages = {352 -- 357}, year = {2010}, language = {en} } @inproceedings{Goldbach2010, author = {Goldbach, Daniel}, title = {Management der funktionalen Sicherheit einer Entwicklung eines sicherheitskritischen Systems im Bereich Nutzfahrzeuge}, series = {Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)}, booktitle = {Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)}, publisher = {Shaker}, address = {Aachen}, isbn = {978-3-8322-9040-5}, pages = {487 -- 495}, year = {2010}, language = {de} } @article{Goldbach2010, author = {Goldbach, Daniel}, title = {Fahrstabilit{\"a}tserh{\"o}hung bei Gespannen durch Querverschiebung des Anh{\"a}ngerkupplungspunktes}, series = {mechatronik mobil}, volume = {2}, journal = {mechatronik mobil}, number = {1}, publisher = {Expert Verlag}, address = {Renningen}, issn = {1867-7371}, pages = {23 -- 29}, year = {2010}, language = {de} } @inproceedings{ReisgenSchleserMokrovetal.2010, author = {Reisgen, Uwe and Schleser, Markus and Mokrov, Oleg and Schmidt, A. and Rossiter, E. and Loose, T.}, title = {Durchg{\"a}ngige Schweißsimulation der Eigenspannung und des Gef{\"u}gezustands auf Basis realer Schweißstromquellenparameter}, series = {DVS congress 2010 : Große Schweißtechnische Tagung ; Studentenkongress ; Statusseminar "Schneidtechnik" ; Abschlusskolloquium zum AiF-Forschungscluster "Integration des R{\"u}hrreibschweißens in Fertigungsprozessketten" ; Vortr{\"a}ge der Veranstaltung in N{\"u}rnberg am 27. und 28. September 2010. (DVS-Berichte ; 267)}, booktitle = {DVS congress 2010 : Große Schweißtechnische Tagung ; Studentenkongress ; Statusseminar "Schneidtechnik" ; Abschlusskolloquium zum AiF-Forschungscluster "Integration des R{\"u}hrreibschweißens in Fertigungsprozessketten" ; Vortr{\"a}ge der Veranstaltung in N{\"u}rnberg am 27. und 28. September 2010. (DVS-Berichte ; 267)}, publisher = {DVS Media}, address = {D{\"u}sseldorf}, organization = {Deutscher Verband f{\"u}r Schweißen und Verwandte Verfahren ; Große Schweißtechnische Tagung <2010, N{\"u}rnberg>}, isbn = {978-3-87155-592-3}, pages = {37 -- 42}, year = {2010}, language = {de} } @inproceedings{PompSchwarzboezlKolletal.2010, author = {Pomp, Stefan and Schwarzb{\"o}zl, Peter and Koll, Gerrit and Hennecke, Klaus and Schmitz, Mark and Hoffschmidt, Bernhard}, title = {Advanced concept for a solar thermal power plant with open volumetric air receiver}, series = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, booktitle = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, publisher = {Soc. OSC}, address = {Saint Maur}, pages = {97 -- 98}, year = {2010}, language = {en} } @inproceedings{SchwarzboezlPompKolletal.2010, author = {Schwarzb{\"o}zl, Peter and Pomp, Stefan and Koll, Gerrit and Hennecke, Klaus and Hartz, Thomas and Schmitz, Mark and Hoffschmidt, Bernhard}, title = {The solar tower J{\"u}lich - first operational experiences and test results}, series = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, booktitle = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, publisher = {Soc. OSC}, address = {Saint Maur}, pages = {8 -- 9}, year = {2010}, language = {en} } @inproceedings{BuckWurmhoeringerLehleetal.2010, author = {Buck, R. and Wurmh{\"o}ringer, K. and Lehle, R. and Pfahl, A. and G{\"o}ttsche, Joachim and Meyr, T.}, title = {Development of a 30m2 heliostat with hydraulic drive}, series = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, booktitle = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, publisher = {Soc. OSC}, address = {Saint Maur}, pages = {74 -- 75}, year = {2010}, language = {en} } @inproceedings{AlexopoulosHoffschmidtRauetal.2010, author = {Alexopoulos, Spiros and Hoffschmidt, Bernhard and Rau, Christoph and Schmitz, M. and Schwarzb{\"o}zl, P. and Pomp, Stefan}, title = {Simulation results for a hybridized operation of a gas turbine or a burner for a small solar tower power plant}, series = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, booktitle = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, publisher = {Soc. OSC}, address = {Saint Maur}, pages = {82 -- 83}, year = {2010}, language = {en} } @inproceedings{BaumannTeixeiraBouraGoettscheetal.2010, author = {Baumann, T. and Teixeira Boura, Cristiano Jos{\´e} and G{\"o}ttsche, Joachim and Hoffschmidt, Bernhard and O'Connell, B. and Schmitz, S. and Zunft, S.}, title = {Air/Sand heat exchanger design and materials for solar thermal power plant applications}, series = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, booktitle = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, publisher = {Soc. OSC}, address = {Saint Maur}, pages = {146 -- 147}, year = {2010}, language = {en} }