@article{FalkenbergVossBottetal.2023, author = {Falkenberg, Fabian and Voß, Leonie and Bott, Michael and Bongaerts, Johannes and Siegert, Petra}, title = {New robust subtilisins from halotolerant and halophilic Bacillaceae}, series = {Applied Microbiology and Biotechnology}, volume = {107}, journal = {Applied Microbiology and Biotechnology}, publisher = {Springer Nature}, address = {Berlin}, issn = {1432-0614}, doi = {10.1007/s00253-023-12553-w}, pages = {3939 -- 3954}, year = {2023}, abstract = {The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0-12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5\% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications.}, language = {en} } @inproceedings{GellertNordaButenweg2008, author = {Gellert, Christoph and Norda, Hannah and Butenweg, Christoph}, title = {Nonlinear behaviour of masonry under cyclic loading}, series = {7th European Conference on Structural Dynamics, EURODYN 2008 : 7-9 July 2008, Southampton}, booktitle = {7th European Conference on Structural Dynamics, EURODYN 2008 : 7-9 July 2008, Southampton}, organization = {European Conference on Structural Dynamics, EURODYN <7, 2008, Southampton>}, pages = {1 -- 12}, year = {2008}, language = {en} } @article{HaegerProbstJaegeretal.2023, author = {Haeger, Gerrit and Probst, Johanna and Jaeger, Karl-Erich and Bongaerts, Johannes and Siegert, Petra}, title = {Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans}, series = {FEBS Open Bio}, volume = {13}, journal = {FEBS Open Bio}, number = {12}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {2211-5463}, doi = {10.1002/2211-5463.13723}, pages = {2224 -- 2238}, year = {2023}, abstract = {Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.}, language = {en} } @inproceedings{AridaKloockSchoening2006, author = {Arida, Hassan A. and Kloock, Joachim P. and Sch{\"o}ning, Michael Josef}, title = {Novel organic membrane-based thin-film microsensors for the determination of heavy metal cations}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-1545}, year = {2006}, abstract = {A first step towards the fabrication and electrochemical evaluation of thin-film microsensors based on organic PVC membranes for the determination of Hg(II), Cd(II), Pb(II) and Cu(II) ions in solutions has been realised. The membrane-coating mixture used in the preparation of this new type of microsensors is incorporating PVC as supporting matrix, o-nitrophenyloctylether (o-NPOE) as solvent mediator and a recently synthesized Hg[dimethylglyoxime(phene)]2+ and Bis-(4-hydroxyacetophenone)-ethylenediamine as electroactive materials for Hg(II) and Cd(II), respectively. A set of three commercialised ionophores for Cd(II), Pb(II) and Cu(II) has been also used for comparison. Thin-film microsensors based on these membranes showed a Nernstian response of slope (26-30 mV/dec.) for the respective tested cations. The potentiometric response characteristics (linear range, pH range, detection limit and response time) are comparable with those obtained by conventional membranes as well as coated wire electrodes prepared from the same membrane. The realisation of the new organic membrane-based thin-film microsensors overcomes the problem of an insufficient selectivity of solid-state-based thinfilm sensors.}, subject = {Biosensor}, language = {en} } @article{HaegerJolmesOyenetal.2024, author = {Haeger, Gerrit and Jolmes, Tristan and Oyen, Sven and Jaeger, Karl-Erich and Bongaerts, Johannes and Sch{\"o}rken, Ulrich and Siegert, Petra}, title = {Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis}, series = {Applied Microbiology and Biotechnology}, journal = {Applied Microbiology and Biotechnology}, number = {108}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614}, doi = {10.1007/s00253-023-12868-8}, pages = {14 Seiten}, year = {2024}, abstract = {N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75\%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC-MS and NMR.}, language = {en} } @techreport{Wessling2006, type = {Working Paper}, author = {Weßling, Matthias}, title = {O Perfil Reiss: Conceito scientifico e conclus{\~o}es da pesquisa, aplica{\c{c}}{\~o}es exemplaricas e conseq{\"u}{\^e}ncias pr{\´a}ticas}, year = {2006}, abstract = {Para Reiss, a avalia{\c{c}}{\~a}o de cada um dos 16 motivos vitais {\´e} a chave para n{\~a}o s{\´o} entender o comportamento humano, como tamb{\´e}m para poder prev{\^e}-lo. Tal previs{\~a}o {\´e} tamb{\´e}m a meta de cada ci{\^e}ncia. Quando se quer saber a rea{\c{c}}{\~a}o das pessoas, precisa-se primeiro descobrir, o que as pessoas realmente querem - e, ent{\~a}o prever, que elas satisfar{\~a}o esses desejos e necessidades em suas a{\c{c}}{\~o}es. As chances, que o Perfil Reiss oferece, resultam entre outras tamb{\´e}m, que aqui {\´e} poss{\´i}vel pela primeira vez uma individualiza{\c{c}}{\~a}o total. Cerca de 6 bilh{\~o}es de perfis individuais podem ser apresentados com o Perfil Reiss. Na combina{\c{c}}{\~a}o correta da forma{\c{c}}{\~a}o e utiliza{\c{c}}{\~a}o da tipologia de um lado e da individualidade da conclus{\~a}o de outro lado, est{\´a} a for{\c{c}}a positiva desse instrumento.}, subject = {Motivation}, language = {pt} } @masterthesis{Vu2022, type = {Bachelor Thesis}, author = {Vu, Tuan Dat}, title = {Objekterkennung und Schienenerkennung in der Schienenfahrzeugtechnik}, publisher = {FH Aachen}, address = {Aachen}, school = {Fachhochschule Aachen}, pages = {72 Seiten}, year = {2022}, language = {de} } @inproceedings{Bung2014, author = {Bung, Daniel Bernhard}, title = {Observations on non-aerated flow and air entrainment on moderately sloped stepped spillways}, series = {7th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : 8. - 12. Sept. 2014, Hannover}, booktitle = {7th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : 8. - 12. Sept. 2014, Hannover}, organization = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <7, 2014, Hannover>}, pages = {1 -- 8}, year = {2014}, language = {en} } @article{MoehrenBergmannJanseretal.2023, author = {M{\"o}hren, Felix and Bergmann, Ole and Janser, Frank and Braun, Carsten}, title = {On the influence of elasticity on propeller performance: a parametric study}, series = {CEAS Aeronautical Journal}, volume = {14}, journal = {CEAS Aeronautical Journal}, publisher = {Springer Nature}, address = {Berlin}, issn = {1869-5590 (Online)}, doi = {10.1007/s13272-023-00649-y}, pages = {311 -- 323}, year = {2023}, abstract = {The aerodynamic performance of propellers strongly depends on their geometry and, consequently, on aeroelastic deformations. Knowledge of the extent of the impact is crucial for overall aircraft performance. An integrated simulation environment for steady aeroelastic propeller simulations is presented. The simulation environment is applied to determine the impact of elastic deformations on the aerodynamic propeller performance. The aerodynamic module includes a blade element momentum approach to calculate aerodynamic loads. The structural module is based on finite beam elements, according to Timoshenko theory, including moderate deflections. Several fixed-pitch propellers with thin-walled cross sections made of both isotropic and non-isotropic materials are investigated. The essential parameters are varied: diameter, disc loading, sweep, material, rotational, and flight velocity. The relative change of thrust between rigid and elastic blades quantifies the impact of propeller elasticity. Swept propellers of large diameters or low disc loadings can decrease the thrust significantly. High flight velocities and low material stiffness amplify this tendency. Performance calculations without consideration of propeller elasticity can lead to decreased efficiency. To avoid cost- and time-intense redesigns, propeller elasticity should be considered for swept planforms and low disc loadings.}, language = {en} } @masterthesis{Kauffmann2023, type = {Bachelor Thesis}, author = {Kauffmann, Michael}, title = {OpenCouch : Therapiesuche leicht gemacht.}, publisher = {FH Aachen}, address = {Aachen}, school = {Fachhochschule Aachen}, pages = {175 Seiten}, year = {2023}, abstract = {Die Auswirkungen psychischer Erkrankungen stellen ein zunehmendes Thema in unserer Gesellschaft dar. Allein in Deutschland erkranken jedes Jahr ein Viertel aller Menschen psychisch. Besonders seit der Pandemie w{\"a}chst die Nachfrage nach psychotherapeutischer Behandlung stetig an, wodurch die Wartelisten in den Praxen immer l{\"a}nger werden. Betroffene m{\"u}ssen oft ein halbes Jahr oder l{\"a}nger auf einen Therapieplatz warten und sind gezwungen zu nehmen, was gerade frei ist. Dabei ist es f{\"u}r den Therapieerfolg essenziell, dass nicht nur die Therapie auf einen abgestimmt ist, sondern auch die Chemie zwischen Therapeut:in und Patient:in passt. OpenCouch ist eine Anwendung, die Betroffene bei der Therapieplatzsuche unterst{\"u}tzen soll, indem sie Informationen und Tools zur Verf{\"u}gung stellt, um trotz der geringen Verf{\"u}gbarkeit einen Therapieplatz finden zu k{\"o}nnen, der einen Behandlungserfolg garantiert.}, language = {de} } @article{CheenakulaPaulsenOttetal.2023, author = {Cheenakula, Dheeraja and Paulsen, Svea and Ott, Fabian and Gr{\"o}mping, Markus}, title = {Operational window of a deammonifying sludge for mainstream application in a municipal wastewater treatment plant}, series = {Water and Environment Journal}, volume = {38}, journal = {Water and Environment Journal}, number = {1}, publisher = {Wiley}, address = {Chichester}, issn = {1747-6593}, doi = {10.1111/wej.12898}, pages = {59 -- 70}, year = {2023}, abstract = {The present work aimed to study the mainstream feasibility of the deammonifying sludge of side stream of municipal wastewater treatment plant (MWWTP) in Kaster, Germany. For this purpose, the deammonifying sludge available at the side stream was investigated for nitrogen (N) removal with respect to the operational factors temperature (15-30°C), pH value (6.0-8.0) and chemical oxygen demand (COD)/N ratio (≤1.5-6.0). The highest and lowest N-removal rates of 0.13 and 0.045 kg/(m³ d) are achieved at 30 and 15°C, respectively. Different conditions of pH and COD/N ratios in the SBRs of Partial nitritation/anammox (PN/A) significantly influenced both the metabolic processes and associated N-removal rates. The scientific insights gained from the current work signifies the possibility of mainstream PN/A at WWTPs. The current study forms a solid basis of operational window for the upcoming semi-technical trails to be conducted prior to the full-scale mainstream PN/A at WWTP Kaster and WWTPs globally.}, language = {en} } @inproceedings{KraftRetkowitz2005, author = {Kraft, Bodo and Retkowitz, Daniel}, title = {Operationale Semantikdefinition f{\"u}r konzeptuelles Regelwissen}, year = {2005}, abstract = {In: Forum Bauinformatik 2005 : junge Wissenschaftler forschen / [Lehrstuhl Bauinformatik, Brandenburgische Technische Universit{\"a}t Cottbus. Frank Schley ... (Hrsg.)]. - Cottbus : Techn. Universit{\"a}t 2005. S. 1-10 ISBN 3-934934-11-0 Mittels eines operationalen Ansatzes zur Semantikdefinition wird am Bei-spiel des konzeptuellen Geb{\"a}udeentwurfs ein Regelsystem formalisiert. Dazu werdenzwei Teile, zum einen das Regelwissen, zum anderen ein konzeptueller Entwurfsplan zun{\"a}chst informell eingef{\"u}hrt und dann formal beschrieben. Darauf aufbauend wird die Grundlage f{\"u}r eine Konsistenzpr{\"u}fung des konzeptuellen Entwurfs gegen das Regel-wissen formal angeben}, subject = {CAD}, language = {de} } @misc{Bellenberg2002, author = {Bellenberg, Markus}, title = {Optimierung einer Roboter-Schweißzelle als Basis eines E-Learning Systems}, year = {2002}, abstract = {Ziel war die technische {\"U}berarbeitung und Optimierung des vorliegenden Prototypen, um eine Basis f{\"u}r ein E-Learning System (Internet unterst{\"u}tztes Lernen) zu schaffen. Zu diesem Zweck wurde das Programm der Robotersteuerung neu erstellt, notwendige mechanische {\"A}nderungen an Roboterequipment und Materialf{\"o}rderung vorgenommen sowie eine neue Internet-Anbindung geschaffen. Wesentliche Ergebnisse der Arbeit sind: • Erzeugen roboterbasierter kreisinterpolierter Bahnschweißn{\"a}hte • Kommunikation des Lernsystems via Internet • Lernorientierte Steuerungssoftware}, subject = {Roboter}, language = {de} } @inproceedings{JungStaatMueller2014, author = {Jung, Alexander and Staat, Manfred and M{\"u}ller, Wolfram}, title = {Optimization of the flight style in ski jumping}, series = {11th World Congress on Computational Mechanics (WCCM XI) ; 5th European Conference on Computational Mechanics (ECCM V) ; 6th European Conference on Computational Fluid Dynamics (ECFD VI) ; July 20 - 25, 2014, Barcelona}, booktitle = {11th World Congress on Computational Mechanics (WCCM XI) ; 5th European Conference on Computational Mechanics (ECCM V) ; 6th European Conference on Computational Fluid Dynamics (ECFD VI) ; July 20 - 25, 2014, Barcelona}, editor = {Onate, E.}, organization = {World Congress on Computational Mechanics <11, 2014, Barcelona>}, pages = {799 -- 810}, year = {2014}, language = {en} } @misc{OPUS4-10963, title = {Ordnung zur Aufhebung der Pr{\"u}fungsordnung f{\"u}r den Masterstudiengang „Elektrotechnik" (zuvor „Nachrichtentechnik" und „Elektrotechnik und Informationstechnik") vom 22. Januar 2010 (FH-Mitteilung Nr. 7/2010), zuletzt ge{\"a}ndert durch {\"A}nderungsordnung vom 25. April 2016 (FH-Mitteilung Nr. 31/2016) : vom 20. Dezember 2019}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2019}, language = {de} } @misc{OPUS4-10962, title = {Ordnung zur Aufhebung der Pr{\"u}fungsordnung f{\"u}r den Masterstudiengang „Information Systems Engineering" vom 22. Januar 2010 (FH-Mitteilung Nr. 5/2010), zuletzt ge{\"a}ndert durch {\"A}nderungsordnung vom 4. Februar 2013 (FH-Mitteilung Nr. 6/2013) : vom 20. Dezember 2019}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2019}, language = {de} } @misc{OPUS4-10964, title = {Ordnung zur Aufhebung der Pr{\"u}fungsordnung f{\"u}r die Masterstudieng{\"a}nge „Energiewirtschaft \& Informatik" (3 Semester) und „Energiewirtschaft \& Informatik" (4 Semester) vom 4. Mai 2016 (FH-Mitteilung Nr. 59/2016) Fachbereich Energietechnik Fachbereich Medizintechnik und Technomathematik an der Fachhochschule Aachen : vom 16. Januar 2020}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2020}, language = {de} } @misc{OPUS4-10956, title = {Ordnung zur Aufhebung der Pr{\"u}fungsordnungen f{\"u}r den Masterstudiengang „Nuclear Applications" vom 13. Februar 2004 (FH-Mitteilung Nr. 5/2004) und vom 25. Februar 2010 (FH-Mitteilung Nr. 15/2010) in den Fassungen der Bekanntmachung der {\"A}nderungsordnungen vom 6. November 2012 (FH-Mitteilung Nr. 120/2012), 18. Juni 2014 (FH- Mitteilung Nr. 72/2014) und vom 15. Juni 2015 (FH-Mitteilung Nr. 28/2015) : vom 29. November 2019}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2019}, language = {de} } @misc{OPUS4-10877, title = {Ordnung zur Aufhebung der Studien- und Pr{\"u}fungsordnung f{\"u}r den Bachelorstudiengang „Holzingenieurwesen" (7-semestrig) vom 16. Juli 2012 (FH-Mitteilung Nr. 66/2012) in der Fassung der Bekanntmachung der {\"A}nderungsordnungen vom 6. Juli 2016 (FH-Mitteilung Nr. 95/2016) und 16. August 2019 (FH-Mitteilung Nr. 76/2019) an der Fachhochschule Aachen : vom 24. Juli 2018 - FH-Mitteilung Nr. 120/2018 in der Fassung der Bekanntmachung der {\"A}nderungsordnung vom 16. August 2019 - FH-Mitteilung Nr. 77/2019 (Nichtamtliche lesbare Fassung)}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2019}, language = {de} } @misc{OPUS4-10883, title = {Ordnung zur Aufhebung der Studien- und Pr{\"u}fungsordnung f{\"u}r den Masterstudiengang „Bauingenieurwesen" vom 10. Dezember 2012 (FH-Mitteilung Nr. 129/2012) in der Fassung der Bekanntmachung der {\"A}nderungsordnung vom 30. Mai 2017 (FH-Mitteilung Nr. 58/2017) und 16. August 2019 (FH-Mitteilung Nr. 80/2019) an der Fachhochschule Aachen : vom 24. Juli 2018 - FH-Mitteilung Nr. 121/2018 in der Fassung der Bekanntmachung der {\"A}nderungsordnung vom 16. August 2019 - FH-Mitteilung Nr. 81/2019 (Nichtamtliche lesbare Fassung)}, organization = {FH Aachen}, pages = {3 Seiten}, year = {2019}, language = {de} }