@article{WuBronderPoghossianetal.2015, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Werner, Frederik and Sch{\"o}ning, Michael Josef}, title = {Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer}, series = {Nanoscale}, volume = {14}, journal = {Nanoscale}, number = {7}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, doi = {10.1039/C4NR07225A}, pages = {6143 -- 6150}, year = {2015}, abstract = {A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent-voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.}, language = {en} } @incollection{PoghossianSchoening2006, author = {Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Silicon-based chemical and biological field-effect sensors}, series = {Encyclopedia of Sensors. Vol. 9 S - Sk}, booktitle = {Encyclopedia of Sensors. Vol. 9 S - Sk}, publisher = {ASP, American Scientific Publ.}, address = {Stevenson Ranch, Calif.}, isbn = {1-58883-065-9}, pages = {463 -- 534}, year = {2006}, language = {en} } @inproceedings{BegingPoghossianMlyneketal.2010, author = {Beging, Stefan and Poghossian, Arshak and Mlynek, D. and Hataihimakul, S. and Pedraza, A. and Dhawan, S. and Laube, N. and Kleinen, L. and Baldsiefen, G. and Busch, H. and Sch{\"o}ning, Michael Josef}, title = {Ion-selective sensors for the determination of the risk of urinary stone formation}, series = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, booktitle = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, address = {Warsaw}, pages = {74 -- 80}, year = {2010}, language = {en} } @inproceedings{NaetherPoghossianPlatenetal.2006, author = {N{\"a}ther, Niko and Poghossian, Arshak and Platen, J. and Yoshinobu, T. and Koudelka-Hep, M. and Sch{\"o}ning, Michael Josef}, title = {Multi-parameter sensing of both physical and (bio-)chemical quantities using the same transducer principle}, series = {Biochemical sensing utilisation of micro- and nanotechnologies : Warsaw, [23rd - 26th] November 2005 / ed. by M. Mascini ...}, booktitle = {Biochemical sensing utilisation of micro- and nanotechnologies : Warsaw, [23rd - 26th] November 2005 / ed. by M. Mascini ...}, address = {Warsaw}, pages = {172 -- 181}, year = {2006}, language = {en} } @inproceedings{SchoeningAbouzarWagneretal.2006, author = {Sch{\"o}ning, Michael Josef and Abouzar, Maryam H. and Wagner, Torsten and N{\"a}ther, Niko and Rolka, David and Yoshinobu, Tatsuo and Kloock, Joachim P. and Turek, Monika and Ingebrandt, Sven and Poghossian, Arshak}, title = {A semiconductor-based field-effect platform for (bio-)chemical and physical sensors: From capacitive EIS sensors and LAPS over ISFETs to nano-scale devices}, series = {MRS Proceedings}, booktitle = {MRS Proceedings}, doi = {10.1557/PROC-0952-F08-02}, pages = {1 -- 9}, year = {2006}, language = {en} } @article{SchoeningPoghossian2006, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {BioFEDs (field-effect devices) : State-of-the-art and new directions}, series = {Electroanalysis}, volume = {18}, journal = {Electroanalysis}, number = {19-20}, issn = {1521-4109}, doi = {10.1002/elan.200603609}, pages = {1893 -- 1900}, year = {2006}, language = {en} } @inproceedings{PlatenPoghossianSchoening2006, author = {Platen, J. and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Pr{\"a}paration von selbstjustierenden Nanostrukturen mittels Schichtausdehnungstechnik}, series = {Sensoren und Mess-Systeme 2006 : Vortr{\"a}ge der 13. ITG/GMA-Fachtagung vom 13. bis 14.3.2006 in Freiburg/Breisgau}, booktitle = {Sensoren und Mess-Systeme 2006 : Vortr{\"a}ge der 13. ITG/GMA-Fachtagung vom 13. bis 14.3.2006 in Freiburg/Breisgau}, publisher = {VDE Verl.}, address = {Berlin}, isbn = {3-8007-2939-3}, pages = {277 -- 280}, year = {2006}, language = {de} } @inproceedings{SchoeningAbouzarHanetal.2006, author = {Sch{\"o}ning, Michael Josef and Abouzar, Maryam H. and Han, Y. and Ingebrandt, S. and Offenh{\"a}usser, A. and Poghossian, Arshak}, title = {Markierungsfreie DNA-Detektion mit Silizium-Feldeffektsensoren - Messeffekte oder Artefakte?}, series = {Sensoren und Mess-Systeme 2006 : Vortr{\"a}ge der 13. ITG/GMA-Fachtagung vom 13. bis 14.3.2006 in Freiburg/Breisgau}, booktitle = {Sensoren und Mess-Systeme 2006 : Vortr{\"a}ge der 13. ITG/GMA-Fachtagung vom 13. bis 14.3.2006 in Freiburg/Breisgau}, publisher = {VDE Verl.}, address = {Berlin}, isbn = {3-8007-2939-3}, pages = {443 -- 446}, year = {2006}, language = {de} } @inproceedings{HuckBaeckerPoghossianetal.2011, author = {Huck, Christina and B{\"a}cker, Matthias and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Bifunktionaler Sensorchip f{\"u}r den Nachweis von Gel{\"o}stwasserstoff}, isbn = {978-3942710-53-4}, doi = {10.5162/10dss2011/16.10}, pages = {325 -- 328}, year = {2011}, language = {de} } @inproceedings{WeilPoghossianSchoeningetal.2012, author = {Weil, M. and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Cherstvy, A.}, title = {Electrical monitoring of layer-by-layer adsorption of oppositely charged macromolecules by means of capacitive field-effect devices}, isbn = {978-3-9813484-2-2}, doi = {10.5162/IMCS2012/P2.5.2}, pages = {1575 -- 1578}, year = {2012}, language = {en} }