@article{HaegerJolmesOyenetal.2024,
  author    = {Haeger, Gerrit and Jolmes, Tristan and Oyen, Sven and Jaeger, Karl-Erich and Bongaerts, Johannes and Sch{\"o}rken, Ulrich and Siegert, Petra},
  title     = {Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis},
  series = {Applied Microbiology and Biotechnology},
  journal   = {Applied Microbiology and Biotechnology},
  number    = {108},
  publisher = {Springer},
  address   = {Berlin},
  issn      = {1432-0614},
  doi       = {10.1007/s00253-023-12868-8},
  pages     = {14 Seiten},
  year      = {2024},
  abstract  = {N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75\%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC-MS and NMR.},
  language  = {en}
}
@masterthesis{Liedtke2024,
  type      = {Bachelor Thesis},
  author    = {Liedtke, Janna},
  title     = {Lehrmaterial f{\"u}r den Tiroler Skilehrerverband: Konzeption f{\"u}r eine crossmediale Neugestaltung},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {68 Seiten},
  year      = {2024},
  abstract  = {Der Tiroler Skilehrerverband bereitet angehende Skilehrer:innen auf die verschiedenen Schneesportlehrer-Pr{\"u}fungen vor. Skilehrer:innen spielen eine entscheidende Rolle im Erfolg des Wintertourismus, was ihre gesellschaftliche und wirtschaftliche Relevanz unterstreicht. Die zeitgem{\"a}ße Neugestaltung des Lehrmaterials soll eine effektive Vermittlung von Lerninhalten erm{\"o}glichen. Durch die Kombination von Informationsdesign, Corporate Design und Editorial Design wird die Verst{\"a}ndlichkeit und Attraktivit{\"a}t des Materials gesteigert. Die Integration einer Lernapp erm{\"o}glicht eine interaktive und multimediale Lernerfahrung sowie mehr Flexibilit{\"a}t f{\"u}r die Lernenden. Die crossmediale Neugestaltung wirkt den Problemen veralteter Darstellungsformen entgegen. Es wird deutlich, dass gut gestaltetes Lehrmaterial bei gleichbleibenden Inhalten zu einer besseren Lernerfahrung f{\"u}hrt.},
  language  = {de}
}
@masterthesis{Jansen2024,
  type      = {Bachelor Thesis},
  author    = {Jansen, Marie},
  title     = {Budzi Budzi},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {53 Seiten},
  year      = {2024},
  abstract  = {Mit {\"u}ber 1300 B{\"u}dchen in 86 Veedeln ist K{\"o}ln Deutschlands Kiosk-Hauptstadt. Sie sind ein pr{\"a}gendes Element von Nachbarschaften und geh{\"o}ren zum K{\"o}lner Stadtbild einfach dazu wie der Dom und der Rhein. Durch Anekdoten, Interviews, Beitr{\"a}ge zur Popkultur, alte Fotografien, Momentaufnahmen und vertraute Grafiken sowie einer festen Formsprache entsteht das erste B{\"u}dchenmagazin f{\"u}r K{\"o}ln. Das Periodikum erscheint alle drei Monate und liegt kostenfrei in den B{\"u}dchen aus. Es ist eine Publikation die beweist, dass B{\"u}dchen mehr als g{\"a}ngige Verkaufsstellen sind, sondern Knotenpunkte unserer Gesellschaft.},
  language  = {de}
}