@misc{KluinMaurerBanowskietal.2005,
  author    = {Kluin, Cornelia and Maurer, Karl-Heinz and Banowski, Bernhard and Bessler, Cornelius and Siegert, Petra},
  title     = {Verwendung von Siderophoren gegen Geruchskeime [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt},
  address   = {M{\"u}nchen},
  pages     = {1 -- 32},
  year      = {2005},
  language  = {de}
}
@misc{MerkelWeberSiegertetal.2006,
  author    = {Merkel, Marion and Weber, Angrit and Siegert, Petra and Wieland, Susanne and Maurer, Karl-Heinz and Bessler, Cornelius},
  title     = {Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 46},
  year      = {2006},
  language  = {de}
}
@misc{BesslerMaurerMerkeletal.2007,
  author    = {Bessler, Cornelius and Maurer, Karl-Heinz and Merkel, Marion and Siegert, Petra and Wieland, Susanne},
  title     = {Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / Europ{\"a}isches Patentamt / WIPO / CIPO},
  address   = {M{\"u}nchen / Den Hague / Genf},
  pages     = {1 -- 47},
  year      = {2007},
  language  = {de}
}
@misc{O'ConnellHovenSiegertetal.2007,
  author    = {O'Connell, Timothy and Hoven, Nina and Siegert, Petra and Maurer, Karl-Heinz},
  title     = {Amadoriasen in Wasch- und Reinigungsmitteln [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / Europ{\"a}isches Patentamt / WIPO},
  address   = {M{\"u}nchen / Den Hague / Genf},
  pages     = {1 -- 45},
  year      = {2007},
  language  = {de}
}
@misc{SiegertWielandEngelskirchenetal.2008,
  author    = {Siegert, Petra and Wieland, Susanne and Engelskirchen, Julia and Merkel, Marion and Maurer, Karl-Heinz and Bessler, Cornelius},
  title     = {Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift]},
  publisher = {Deutsches Patentamt / Europ{\"a}isches Patentamt / WIPO},
  address   = {M{\"u}nchen / Den Hague / Genf},
  pages     = {1 -- 51},
  year      = {2008},
  language  = {de}
}
@misc{BesslerEversMaureretal.2009,
  author    = {Bessler, Cornelius and Evers, Stefan and Maurer, Karl-Heinz and Merkel, Marion and Siegert, Petra and Weber, Angrit and Wieland, Susanne},
  title     = {Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 41},
  year      = {2009},
  language  = {de}
}
@misc{BesslerMaurerMerkeletal.2009,
  author    = {Bessler, Cornelius and Maurer, Karl-Heinz and Merkel, Marion and Siegert, Petra and Wieland, Susanne},
  title     = {Subtilisin from Bacillus Pumilus and detergent and cleaning agents containing said novel subtilisin [US Patentanmeldung / Internationale Patentanmeldung]},
  publisher = {USPTO; WIPO},
  address   = {Washington; Genf},
  pages     = {1 -- 39},
  year      = {2009},
  language  = {en}
}
@misc{SiegertMerkelKluinetal.2009,
  author    = {Siegert, Petra and Merkel, Marion and Kluin, Cornelia and O'Connell, Timothy and Maurer, Karl-Heinz},
  title     = {Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 15},
  year      = {2009},
  language  = {de}
}
@misc{BergBesslerGerlachetal.2009,
  author    = {Berg, Gabriele and Bessler, Cornelius and Gerlach, Jochen and G{\"u}bitz, Georg and Heumann, Sonja and Karl, Wolfgang and Maurer, Karl-Heinz and Remler, Peter and Ribitsch, Doris and Schwab, Helmut and Siegert, Petra and Wieland, Susanne},
  title     = {Mittel enthaltend Proteasen aus Stenotrophomonas maltophilia [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / Europ{\"a}isches Patentamt / USPTO / WIPO},
  address   = {M{\"u}nchen / Den Hague / Washington / Genf},
  pages     = {1 -- 47},
  year      = {2009},
  language  = {de}
}
@article{RibitschKarlBirnerGruenbergeretal.2010,
  author    = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.},
  title     = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli},
  series = {Journal of biotechnology},
  volume    = {150},
  journal   = {Journal of biotechnology},
  number    = {3},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2010.09.947},
  pages     = {408 -- 416},
  year      = {2010},
  abstract  = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.},
  language  = {en}
}