@article{ArtmannTrzewikAtes2002, author = {Artmann, Gerhard and Trzewik, J{\"u}rgen and Ates, M.}, title = {A novel method to quantify mechanical tension in cell monolayers. Trzewik, J{\"u}rgen; Ates, M., Artmann, Gerhard Michael}, series = {Biomedizinische Technik. 47 (2002), H. Suppl. 1. Pt. 1}, journal = {Biomedizinische Technik. 47 (2002), H. Suppl. 1. Pt. 1}, isbn = {0013-5585}, pages = {379 -- 381}, year = {2002}, language = {en} } @article{KurzLinderTrzewiketal.2010, author = {Kurz, R. and Linder, Peter and Trzewik, J{\"u}rgen and R{\"u}ffer, M. and Artmann, Gerhard and Digel, Ilya and Rothermel, A. and Robitzki, A. and Temiz Artmann, Ayseg{\"u}l}, title = {Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes}, series = {Medical and Biological Engineering and Computing}, volume = {48}, journal = {Medical and Biological Engineering and Computing}, number = {1}, publisher = {Springer Nature}, address = {Cham}, issn = {1741-0444}, doi = {10.1007/s11517-009-0552-y}, pages = {59 -- 65}, year = {2010}, abstract = {The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.}, language = {en} } @article{KurulganDemirciLinderDemircietal.2009, author = {Kurulgan Demirci, Eylem and Linder, Peter and Demirci, Taylan and Trzewik, J{\"u}rgen and Digel, Ilya and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Contractile tension of endothelial cells: An LPS based in-vitro sepsis model}, series = {IUBMB Life. 61 (2009), H. 3}, journal = {IUBMB Life. 61 (2009), H. 3}, publisher = {Wiley}, address = {Weinheim}, isbn = {1521-6543}, pages = {307 -- 308}, year = {2009}, language = {en} } @article{TrzewikTemizArtmannLinderetal.2004, author = {Trzewik, J{\"u}rgen and Temiz Artmann, Ayseg{\"u}l and Linder, Peter and Demirci, T. and Digel, Ilya and Artmann, Gerhard}, title = {Evaluation of lateral mechanical tension in thin-film tissue constructs}, series = {Annals of Biomedical Engineering. 32 (2004), H. 9}, journal = {Annals of Biomedical Engineering. 32 (2004), H. 9}, isbn = {1573-9686}, pages = {1243 -- 1251}, year = {2004}, language = {en} } @article{ArtmannTrzewikMallipattuetal.2001, author = {Artmann, Gerhard and Trzewik, J{\"u}rgen and Mallipattu, S. K. and Delano, F. A. [u.a.]}, title = {Evidence for a second valve system in Lymphatics: Endothelial Microvalves. Trzewik, J{\"u}rgen; Mallipattu, S. K.; Artmann, Gerhard Michael; Delano, F. A.; Schmid-Schonbein, G. W}, series = {The FASEB Journal. 15 (2001)}, journal = {The FASEB Journal. 15 (2001)}, isbn = {1530-6860}, pages = {1711 -- 1717}, year = {2001}, language = {en} } @phdthesis{Trzewik2007, author = {Trzewik, J{\"u}rgen}, title = {Experimental analysis of biaxial mechanical tension in cell monolayers and cultured three-dimensional tissues: the celldrum technology}, publisher = {Univerist{\"a}tsverlg Ilmenau}, address = {Ilmenau}, year = {2007}, language = {en} } @inproceedings{DigelDemirciTrzewiketal.2004, author = {Digel, Ilya and Demirci, Taylan and Trzewik, J{\"u}rgen and Linder, Peter and Temiz Artmann, Ayseg{\"u}l}, title = {Fibroblast response to mechanical stress: role of the adhesion substrate : [abstract]}, year = {2004}, abstract = {Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate.}, subject = {Fibroblast}, language = {en} } @article{DemirciKurulganDemirciTrzewiketal.2009, author = {Demirci, Taylan and Kurulgan Demirci, Eylem and Trzewik, J{\"u}rgen and Linder, Peter and Digel, Ilya and Artmann, Gerhard and Sakizli, Meral and Temiz Artmann, Ayseg{\"u}l}, title = {Gene expression profile analysis of 3T3/NIH fibroblasts after one hour mechanical stress}, series = {IUBMB Life. 61 (2009), H. 3}, journal = {IUBMB Life. 61 (2009), H. 3}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {1521-6543}, pages = {311 -- 312}, year = {2009}, language = {en} } @article{KurulganDemirciDemirciTrzewiketal.2011, author = {Kurulgan Demirci, Eylem and Demirci, T. and Trzewik, J{\"u}rgen and Linder, Peter and Karakulah, G. and Artmann, Gerhard and Sakizli, M. and Temiz Artmann, Ayseg{\"u}l}, title = {Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation}, series = {Cellular and molecular bioengineering. 4 (2011), H. 1}, journal = {Cellular and molecular bioengineering. 4 (2011), H. 1}, publisher = {Springer}, address = {Berlin}, isbn = {1865-5025}, pages = {46 -- 55}, year = {2011}, language = {en} } @article{DigelTrzewikDemircietal.2004, author = {Digel, Ilya and Trzewik, J{\"u}rgen and Demirci, Taylan and Temiz Artmann, Ayseg{\"u}l}, title = {Response of fibroblasts to cyclic mechanical stress : a proteome approach / Digel, I. ; Trzewik, J. ; Demirci, T. ; Temiz Artmann, A. ; Artmann, G. M.}, series = {Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2}, journal = {Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2}, isbn = {0932-4666}, pages = {1042 -- 1043}, year = {2004}, language = {en} }