@article{BaeckerBegingBisellietal.2009,
  author    = {B{\"a}cker, Matthias and Beging, Stefan and Biselli, Manfred and Poghossian, Arshak and Wang, J. and Zang, Werner and Wagner, Patrick and Sch{\"o}ning, Michael Josef},
  title     = {Concept for a solid-state multi-parameter sensor system for cell-culture monitoring},
  series = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI},
  journal   = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI},
  publisher = {Elsevier},
  address   = {Amsterdam},
  isbn      = {0013-4686},
  pages     = {6107 -- 6112},
  year      = {2009},
  language  = {en}
}
@article{BaeckerPouyeshmanSchnitzleretal.2011,
  author    = {B{\"a}cker, Matthias and Pouyeshman, S. and Schnitzler, Thomas and Poghossian, Arshak and Wagner, Patrick and Biselli, Manfred and Sch{\"o}ning, Michael Josef},
  title     = {A silicon-based multi-sensor chip for monitoring of fermentation processes},
  series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6},
  journal   = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6},
  publisher = {Wiley},
  address   = {Weinheim},
  isbn      = {1862-6319},
  pages     = {1364 -- 1369},
  year      = {2011},
  language  = {en}
}
@article{BaeckerDellePoghossianetal.2011,
  author    = {B{\"a}cker, Matthias and Delle, L. and Poghossian, Arshak and Biselli, Manfred and Zang, Werner and Wagner, P. and Sch{\"o}ning, Michael Josef},
  title     = {Electrochemical sensor array for bioprocess monitoring},
  series = {Electrochimica Acta (2011)},
  volume    = {56},
  journal   = {Electrochimica Acta (2011)},
  number    = {26},
  publisher = {Elsevier},
  address   = {Amsterdam},
  pages     = {9673 -- 9678},
  year      = {2011},
  language  = {en}
}
@article{WincklerKruegerSchnitzleretal.2014,
  author    = {Winckler, Silvia and Krueger, Rolf and Schnitzler, Thomas and Zang, Werner and Fischer, Rainer and Biselli, Manfred},
  title     = {A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach},
  series = {Bioprocess and biosystems engineering},
  volume    = {37},
  journal   = {Bioprocess and biosystems engineering},
  number    = {5},
  publisher = {Springer},
  address   = {Berlin, Heidelberg},
  issn      = {1615-7591 (Print) 1615-7605 (Online)},
  doi       = {10.1007/s00449-013-1062-8},
  pages     = {901 -- 912},
  year      = {2014},
  abstract  = {Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.},
  language  = {en}
}
@inproceedings{TakenagaHerreraWerneretal.2013,
  author    = {Takenaga, Shoko and Herrera, Cony F. and Werner, Frederik and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten},
  title     = {Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip},
  series = {11. Dresdner Sensor-Symposium : 9.-11.12.2013},
  booktitle = {11. Dresdner Sensor-Symposium : 9.-11.12.2013},
  organization    = {Dresdner Sensor-Symposium <11, 2013>},
  isbn      = {978-3-9813484-5-3},
  pages     = {63 -- 67},
  year      = {2013},
  language  = {en}
}
@article{TakenagaBiselliSchnitzleretal.2014,
  author    = {Takenaga, Shoko and Biselli, Manfred and Schnitzler, Thomas and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten and Sch{\"o}ning, Michael Josef},
  title     = {Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing},
  series = {Physica status solidi A : Applications and materials science},
  volume    = {211},
  journal   = {Physica status solidi A : Applications and materials science},
  number    = {6},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)},
  doi       = {10.1002/pssa.201330464},
  pages     = {1410 -- 1415},
  year      = {2014},
  abstract  = {The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.},
  language  = {en}
}
@article{SchusserBaeckerKrischeretal.2014,
  author    = {Schusser, Sebastian and B{\"a}cker, Matthias and Krischer, M. and Wenzel, L. and Leinhos, Marcel and Poghossian, Arshak and Biselli, Manfred and Wagner, P. and Sch{\"o}ning, Michael Josef},
  title     = {Enzymatically catalyzed degradation of biodegradable polymers investigated by means of a semiconductor-based field-effect sensor},
  series = {Procedia Engineering},
  volume    = {87},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2014.11.689},
  pages     = {1314 -- 1317},
  year      = {2014},
  abstract  = {A semiconductor field-effect device has been used for an enzymatically catalyzed degradation of biopolymers for the first time. This novel technique is capable to monitor the degradation process of multiple samples in situ and in real-time. As model system, the degradation of the biopolymer poly(D, L-lactic acid) has been monitored in the degradation medium containing the enzyme lipase from Rhizomucor miehei. The obtained results demonstrate the potential of capacitive field-effect sensors for degradation studies of biodegradable polymers.},
  language  = {en}
}
@article{TakenagaSchneiderErbayetal.2015,
  author    = {Takenaga, Shoko and Schneider, Benno and Erbay, E. and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and Wagner, Torsten},
  title     = {Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process},
  series = {Physica status solidi (a)},
  volume    = {212},
  journal   = {Physica status solidi (a)},
  number    = {6},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1862-6319},
  doi       = {10.1002/pssa.201532053},
  pages     = {1347 -- 1352},
  year      = {2015},
  abstract  = {A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.},
  language  = {en}
}
@misc{WandreyBiselliSchroederetal.1999,
  author    = {Wandrey, Christian and Biselli, Manfred and Schr{\"o}der, Bernd and Schmoll, Hans-Joachim},
  title     = {Culturing cells on macroporous glass carriers coated with gelatin, extracellular matrix protein and stromal cells},
  pages     = {1 -- 12},
  year      = {1999},
  language  = {en}
}
@article{BaeckerRakowskiPoghossianetal.2013,
  author    = {B{\"a}cker, Matthias and Rakowski, D. and Poghossian, Arshak and Biselli, Manfred and Wagner, Patrick and Sch{\"o}ning, Michael Josef},
  title     = {Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis},
  series = {Journal of Biotechnology},
  volume    = {163},
  journal   = {Journal of Biotechnology},
  number    = {4},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0168-1656},
  doi       = {10.1016/j.jbiotec.2012.03.014},
  pages     = {371 -- 376},
  year      = {2013},
  abstract  = {A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.},
  language  = {en}
}