@article{MourzinaMaiPoghossianetal.2003, author = {Mourzina, Y. and Mai, T. and Poghossian, Arshak and Ermolenko, Y. and Yoshinobu, T. and Vlasov, Y. and Iwasaki, H. and Sch{\"o}ning, Michael Josef}, title = {K+-selective field-effect sensors as transducers for bioelectronic applications}, series = {Electrochimica Acta. 48 (2003), H. 20-22}, journal = {Electrochimica Acta. 48 (2003), H. 20-22}, isbn = {0013-4686}, pages = {3333 -- 3339}, year = {2003}, language = {en} } @article{PoghossianPlatenSchoening2005, author = {Poghossian, Arshak and Platen, J. and Sch{\"o}ning, Michael Josef}, title = {Towards self-aligned nanostructures by means of layerexpansion technique}, series = {Electrochimica Acta. 51 (2005), H. 5}, journal = {Electrochimica Acta. 51 (2005), H. 5}, isbn = {0013-4686}, pages = {838 -- 843}, year = {2005}, language = {en} } @article{MolinnusIkenJohnenetal.2022, author = {Molinnus, Denise and Iken, Heiko and Johnen, Anna Lynn and Richstein, Benjamin and Hellmich, Lena and Poghossian, Arshak and Knoch, Joachim and Sch{\"o}ning, Michael Josef}, title = {Miniaturized pH-Sensitive Field-Effect Capacitors with Ultrathin Ta₂O₅ Films Prepared by Atomic Layer Deposition}, series = {physica status solidi (a) applications and materials science}, volume = {219}, journal = {physica status solidi (a) applications and materials science}, number = {8}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.202100660}, pages = {7 Seiten}, year = {2022}, abstract = {Miniaturized electrolyte-insulator-semiconductor capacitors (EISCAPs) with ultrathin gate insulators have been studied in terms of their pH-sensitive sensor characteristics: three different EISCAP systems consisting of Al-p-Si-Ta2O5(5 nm), Al-p-Si-Si3N4(1 or 2 nm)-Ta2O5 (5 nm), and Al-p-Si-SiO2(3.6 nm)-Ta2O5(5 nm) layer structures are characterized in buffer solution with different pH values by means of capacitance-voltage and constant capacitance method. The SiO2 and Si3N4 gate insulators are deposited by rapid thermal oxidation and rapid thermal nitridation, respectively, whereas the Ta2O5 film is prepared by atomic layer deposition. All EISCAP systems have a clear pH response, favoring the stacked gate insulators SiO2-Ta2O5 when considering the overall sensor characteristics, while the Si3N4(1 nm)-Ta2O5 stack delivers the largest accumulation capacitance (due to the lower equivalent oxide thickness) and a higher steepness in the slope of the capacitance-voltage curve among the studied stacked gate insulator systems.}, language = {en} } @article{BaeckerRaueSchusseretal.2012, author = {B{\"a}cker, Matthias and Raue, Markus and Schusser, Sebastian and Jeitner, C. and Breuer, L. and Wagner, P. and Poghossian, Arshak and F{\"o}rster, Arnold and Mang, Thomas and Sch{\"o}ning, Michael Josef}, title = {Microfluidic chip with integrated microvalves based on temperature- and pH-responsive hydrogel thin films}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100763}, pages = {839 -- 845}, year = {2012}, abstract = {Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3-12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off.}, language = {en} } @article{SchoeningAbouzarPoghossian2009, author = {Sch{\"o}ning, Michael Josef and Abouzar, Maryam H. and Poghossian, Arshak}, title = {pH and ion sensitivity of a field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with polyelectrolyte multilayers}, series = {Journal of Solid State Electrochemistry. 13 (2009), H. 1}, journal = {Journal of Solid State Electrochemistry. 13 (2009), H. 1}, isbn = {1433-0768}, pages = {115 -- 122}, year = {2009}, language = {en} } @article{ThustPoghossianSchoeningetal.1999, author = {Thust, M. and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Naser, S. and M{\"u}ller-Veggian, Mattea and Kordos, P. and L{\"u}th, H.}, title = {Cross-sensitivity of a capacitive penicillin sensor combined with a diffusion barrier}, series = {Proceedings : The Hague, The Netherlands, September 12 - 15, 1999 / [ed. by M. Bartek]. - Vol 1.}, journal = {Proceedings : The Hague, The Netherlands, September 12 - 15, 1999 / [ed. by M. Bartek]. - Vol 1.}, address = {The Hague, The Netherlands}, isbn = {90-76699-02-X}, pages = {573 -- 576}, year = {1999}, language = {en} } @article{BaeckerKowollikSchnitzleretal.2009, author = {B{\"a}cker, Matthias and Kowollik, Silvia and Schnitzler, Thomas and Wang, J. and Poghossian, Arshak and Zang, Werner and Biselli, Manfred and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Entwicklung eines festk{\"o}rperbasierten Sensorsystems f{\"u}r die Bioprozessautomatisierung}, series = {9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]}, journal = {9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]}, publisher = {TUDpress}, address = {Dresden}, isbn = {978-3-941298-44-6}, pages = {197 -- 200}, year = {2009}, language = {de} } @article{SchoeningPoghossianYoshinobuetal.2001, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Yoshinobu, Tatsuo and L{\"u}th, Hans}, title = {Semiconductor-based field-effect structures for chemical sensing}, pages = {188 -- 198}, year = {2001}, language = {en} } @inproceedings{HuckPoghossianBaeckeretal.2013, author = {Huck, Christina and Poghossian, Arshak and B{\"a}cker, Matthias and Zander, W. and Schubert, J. and Sukoyan, L. H. and Begoyan, V. and Buniatyan, V. V. and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Chemische Sensoren mit Bariumstrontiumtitanat als funktionelle Schicht zur Multiparameterdetektion}, series = {11. Dresdner Sensor-Symposium : 9.-11.12.2013}, booktitle = {11. Dresdner Sensor-Symposium : 9.-11.12.2013}, organization = {Dresdner Sensor-Symposium <11, 2013>}, isbn = {978-3-9813484-5-3}, pages = {368 -- 372}, year = {2013}, language = {de} } @inproceedings{SchusserBaeckerLeinhosetal.2013, author = {Schusser, Sebastian and B{\"a}cker, Matthias and Leinhos, Marcel and Krischer, M. and Wenzel, L. and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Sensorkonzept zur in vitro Echtzeitmessung des Degradationsverhaltens von biodegradierbaren Biopolymeren}, series = {11. Dresdner Sensor-Symposium : 9.-11.12.2013}, booktitle = {11. Dresdner Sensor-Symposium : 9.-11.12.2013}, organization = {Dresdner Sensor-Symposium <11, 2013>}, isbn = {978-3-9813484-5-3}, pages = {174 -- 177}, year = {2013}, language = {de} } @article{KochPoghossianSchoeningetal.2018, author = {Koch, Claudia and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Wege, Christian}, title = {Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors}, series = {Nanotheranostics}, volume = {2}, journal = {Nanotheranostics}, number = {2}, publisher = {Ivyspring}, address = {Sydney}, issn = {2206-7418}, doi = {10.7150/ntno.22114}, pages = {184 -- 196}, year = {2018}, abstract = {The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.}, language = {en} } @article{PoghossianThustSchoeningetal.2000, author = {Poghossian, Arshak and Thust, M. and Sch{\"o}ning, Michael Josef and M{\"u}ller-Veggian, Mattea and Kordos, P. and L{\"u}th, H.}, title = {Cross-sensitivity of a capacitive penicillin sensor combined with a diffusion barrier}, series = {Sensors and Actuators B. 68 (2000), H. 1-3}, journal = {Sensors and Actuators B. 68 (2000), H. 1-3}, isbn = {0925-4005}, pages = {260 -- 265}, year = {2000}, language = {en} } @article{SchoeningBrinkmannDemuthetal.2004, author = {Sch{\"o}ning, Michael Josef and Brinkmann, D. and Demuth, C. and Poghossian, Arshak}, title = {CIP (cleaning-in-place)-suitable „non-glass" pH sensor based on a Ta2O5-gate EIS structure}, series = {Digest of technical papers : September 12 - 15, 2004, Rome, Italy, Pontificia Universitas Sancto Thoma Aquinate in Urbe / [conference chairperson: C. Di Natale].}, journal = {Digest of technical papers : September 12 - 15, 2004, Rome, Italy, Pontificia Universitas Sancto Thoma Aquinate in Urbe / [conference chairperson: C. Di Natale].}, address = {Roma}, isbn = {88-7621-282-5}, pages = {857 -- 860}, year = {2004}, language = {en} } @article{PoghossianBerndsenLuethetal.2001, author = {Poghossian, Arshak and Berndsen, Lars and L{\"u}th, Hans and Sch{\"o}ning, Michael Josef}, title = {Novel concepts for flow-rate and flow-direction determination by means of pH-sensitive ISFETs}, series = {Proceedings of SPIE. 4560 (2001)}, journal = {Proceedings of SPIE. 4560 (2001)}, pages = {19 -- 27}, year = {2001}, language = {en} } @article{HonarvarfardGamellaPoghossianetal.2017, author = {Honarvarfard, Elham and Gamella, Maria and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Katz, Evgeny}, title = {An enzyme-based reversible Controlled NOT (CNOT) logic gate operating on a semiconductor transducer}, series = {Applied Materials Today}, volume = {9}, journal = {Applied Materials Today}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2352-9407}, doi = {10.1016/j.apmt.2017.08.003}, pages = {266 -- 270}, year = {2017}, abstract = {An enzyme-based biocatalytic system mimicking operation of a logically reversible Controlled NOT (CNOT) gate has been interfaced with semiconductor electronic transducers. Electrolyte-insulator-semiconductor (EIS) structures have been used to transduce chemical changes produced by the enzyme system to an electronically readable capacitive output signal using field-effect features of the EIS device. Two enzymes, urease and esterase, were immobilized on the insulating interface of EIS structure producing local pH changes performing XOR logic operation controlled by various combinations of the input signals represented by urea and ethyl butyrate. Another EIS transducer was functionalized with esterase only, thus performing Identity (ID) logic operation for the ethyl butyrate input. Both semiconductor devices assembled in parallel operated as a logically reversible CNOT gate. The present system, despite its simplicity, demonstrated for the first time logically reversible function of the enzyme system transduced electronically with the semiconductor devices. The biomolecular realization of a CNOT gate interfaced with semiconductors is promising for integration into complex biomolecular networks and future biosensor/biomedical applications.}, language = {en} } @article{WagnerBegingRotteretal.2007, author = {Wagner, Torsten and Beging, Stefan and Rotter, L. and Poghossian, Arshak and Biselli, Manfred and Zang, Werner and Sch{\"o}ning, Michael Josef}, title = {Online-Messsysteme f{\"u}r die automatisierte Charakterisierung von feldeffektbasierten Biosensoren}, series = {8. Dresdner Sensor-Symposium : Sensoren f{\"u}r Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme f{\"u}r die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung f{\"u}r die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)}, journal = {8. Dresdner Sensor-Symposium : Sensoren f{\"u}r Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme f{\"u}r die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung f{\"u}r die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)}, publisher = {TUDpress, Verl. der Wissenschaften}, address = {Dresden}, isbn = {978-3-940046-45-1}, pages = {257 -- 260}, year = {2007}, language = {de} } @article{YoshinobuEckenPoghossianetal.2001, author = {Yoshinobu, T. and Ecken, H. and Poghossian, Arshak and Simonis, A. and Iwasaki, H. and L{\"u}th, H. and Sch{\"o}ning, Michael Josef}, title = {Constant-current-mode LAPS (CLAPS) for the detection of penicillin}, series = {Electroanalysis. 13 (2001), H. 8-9}, journal = {Electroanalysis. 13 (2001), H. 8-9}, isbn = {1040-0397}, pages = {733 -- 736}, year = {2001}, language = {en} } @article{PoghossianAbouzarSchoening2008, author = {Poghossian, Arshak and Abouzar, Maryam H. and Sch{\"o}ning, Michael Josef}, title = {Capacitance-voltage and impedance characteristics of field-effect EIS sensors functionalised with polyelectrolyte multilayers}, series = {IRBM. 29 (2008), H. 2-3}, journal = {IRBM. 29 (2008), H. 2-3}, isbn = {1959-0318}, pages = {149 -- 154}, year = {2008}, language = {en} } @article{PoghossianLuethSchultzeetal.2001, author = {Poghossian, Arshak and L{\"u}th, H. and Schultze, J. W. and Sch{\"o}ning, Michael Josef}, title = {(Bio-)chemical and physical microsensor array using an identical transducer principle}, series = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, journal = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, publisher = {Elsevier [u.a.]}, address = {Amsterdam [u.a.]}, isbn = {0-08-044014-2}, pages = {243 -- 249}, year = {2001}, language = {en} } @article{SchoeningPoghossian2006, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {BioFEDs (field-effect devices) : State-of-the-art and new directions}, series = {Electroanalysis}, volume = {18}, journal = {Electroanalysis}, number = {19-20}, issn = {1521-4109}, doi = {10.1002/elan.200603609}, pages = {1893 -- 1900}, year = {2006}, language = {en} } @article{HuckPoghossianKerroumietal.2014, author = {Huck, Christina and Poghossian, Arshak and Kerroumi, Iman and Schusser, Sebastian and B{\"a}cker, Matthias and Zander, Willi and Schubert, J{\"u}rgen and Buniatyan, Vahe V. and Martirosyan, Norayr W. and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Multiparameter sensor chip with Barium Strontium Titanate as multipurpose material}, series = {Electroanalysis}, volume = {26}, journal = {Electroanalysis}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-4109 (E-Journal); 1040-0397 (Print)}, doi = {10.1002/elan.201400076}, pages = {980 -- 987}, year = {2014}, abstract = {It is well known that biochemical and biotechnological processes are strongly dependent and affected by a variety of physico-chemical parameters such as pH value, temperature, pressure and electrolyte conductivity. Therefore, these quantities have to be monitored or controlled in order to guarantee a stable process operation, optimization and high yield. In this work, a sensor chip for the multiparameter detection of three physico-chemical parameters such as electrolyte conductivity, pH and temperature is realized using barium strontium titanate (BST) as multipurpose material. The chip integrates a capacitively coupled four-electrode electrolyte-conductivity sensor, a capacitive field-effect pH sensor and a thin-film Pt-temperature sensor. Due to the multifunctional properties of BST, it is utilized as final outermost coating layer of the processed sensor chip and serves as passivation and protection layer as well as pH-sensitive transducer material at the same time. The results of testing of the individual sensors of the developed multiparameter sensor chip are presented. In addition, a quasi-simultaneous multiparameter characterization of the sensor chip in buffer solutions with different pH value and electrolyte conductivity is performed. To study the sensor behavior and the suitability of BST as multifunctional material under harsh environmental conditions, the sensor chip was exemplarily tested in a biogas digestate.}, language = {en} } @article{RolkaPoghossianSchoening2004, author = {Rolka, David and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Integration of a capacitive EIS sensor into a FIA system for pH and penicillin determination}, series = {Sensors. 4 (2004)}, journal = {Sensors. 4 (2004)}, isbn = {1424-8220}, pages = {84 -- 94}, year = {2004}, language = {en} } @article{PoghossianSchoening2004, author = {Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Detecting Both Physical and (Bio-)Chemical Parameters by Means of ISFET Devices}, series = {Electroanalysis. 16 (2004), H. 22}, journal = {Electroanalysis. 16 (2004), H. 22}, isbn = {1040-0397}, pages = {1863 -- 1872}, year = {2004}, language = {en} } @incollection{KochPoghossianWegeetal.2018, author = {Koch, Claudia and Poghossian, Arshak and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {TMV-Based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications}, series = {Virus-Derived Nanoparticles for Advanced Technologies}, booktitle = {Virus-Derived Nanoparticles for Advanced Technologies}, editor = {Wege, Christina}, publisher = {Humana Press}, address = {New York, NY}, isbn = {978-1-4939-7808-3}, doi = {10.1007/978-1-4939-7808-3}, pages = {553 -- 568}, year = {2018}, abstract = {Nanotubular tobacco mosaic virus (TMV) particles and RNA-free lower-order coat protein (CP) aggregates have been employed as enzyme carriers in different diagnostic layouts and compared for their influence on biosensor performance. In the following, we describe a label-free electrochemical biosensor for improved glucose detection by use of TMV adapters and the enzyme glucose oxidase (GOD). A specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CP aggregates was achieved via bioaffinity binding. Glucose sensors with adsorptively immobilized [SA]-GOD, and with [SA]-GOD cross-linked with glutardialdehyde, respectively, were tested in parallel on the same sensor chip. Comparison of these sensors revealed that TMV adapters enhanced the amperometric glucose detection remarkably, conveying highest sensitivity, an extended linear detection range and fastest response times. These results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for applications in biosensorics and biochips. Here, we describe the fabrication and use of amperometric sensor chips combining an array of circular Pt electrodes, their loading with GOD-modified TMV nanotubes (and other GOD immobilization methods), and the subsequent investigations of the sensor performance.}, language = {en} } @article{SiqueiraAbouzarPoghossianetal.2009, author = {Siqueira, Jos{\´e} R. Jr. and Abouzar, Maryam H. and Poghossian, Arshak and Zucolotto, Valtencir and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Penicillin biosensor based on a capacitive field-effect structure functionalized with a dendrimer/carbon nanotube multilayer}, series = {Biosensors and Bioelectronics. 25 (2009), H. 2}, journal = {Biosensors and Bioelectronics. 25 (2009), H. 2}, isbn = {0956-5663}, pages = {497 -- 501}, year = {2009}, language = {en} } @article{GamellaZakharchenkoGuzetal.2017, author = {Gamella, Maria and Zakharchenko, Andrey and Guz, Nataliia and Masi, Madeline and Minko, Sergiy and Kolpashchikov, Dmitry M. and Iken, Heiko and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Katz, Evgeny}, title = {DNA computing system activated by electrochemically triggered DNA realease from a polymer-brush-modified electrode array}, series = {Electroanalysis}, volume = {29}, journal = {Electroanalysis}, number = {2}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-4109}, doi = {10.1002/elan.201600389}, pages = {398 -- 408}, year = {2017}, abstract = {An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAEMA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at -1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals.}, language = {en} } @article{AbouzarPoghossianRazavietal.2008, author = {Abouzar, Maryam H. and Poghossian, Arshak and Razavi, Arash and Besmehn, Astrid and Bijnens, Nathalie and Williams, Oliver A. and Haenen, Ken and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Penicillin detection with nanocrystalline-diamond field-effect sensor}, series = {physica status solidi (a). 205 (2008), H. 9}, journal = {physica status solidi (a). 205 (2008), H. 9}, isbn = {1862-6319}, pages = {2141 -- 2145}, year = {2008}, language = {en} } @inproceedings{HuckBaeckerPoghossianetal.2011, author = {Huck, Christina and B{\"a}cker, Matthias and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Bifunktionaler Sensorchip f{\"u}r den Nachweis von Gel{\"o}stwasserstoff}, isbn = {978-3942710-53-4}, doi = {10.5162/10dss2011/16.10}, pages = {325 -- 328}, year = {2011}, language = {de} } @article{AbouzarIngebrandtPoghossianetal.2009, author = {Abouzar, Maryam H. and Ingebrandt, S. and Poghossian, Arshak and Zhang, Y. and Moritz, W. and Sch{\"o}ning, Michael Josef}, title = {Field-effect nanoplate capacitive pH sensor based on SOI structure}, series = {Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009}, journal = {Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009}, pages = {55 -- 58}, year = {2009}, language = {en} } @article{PoghossianSchoening2003, author = {Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {"High-order" hybrid FET module for (bio)chemical and physical sensing}, series = {Integrated analytical systems / ed. by Salvador Alegret}, journal = {Integrated analytical systems / ed. by Salvador Alegret}, publisher = {Elsevier}, address = {Amsterdam [u.a.]}, isbn = {0-444-51037-0}, pages = {587 -- 623}, year = {2003}, language = {en} } @article{WuBronderPoghossianetal.2015, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Werner, Frederik and Sch{\"o}ning, Michael Josef}, title = {Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer}, series = {Nanoscale}, volume = {14}, journal = {Nanoscale}, number = {7}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, doi = {10.1039/C4NR07225A}, pages = {6143 -- 6150}, year = {2015}, abstract = {A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent-voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.}, language = {en} } @article{BegingMlynekHataihimakuletal.2010, author = {Beging, Stefan and Mlynek, Daniela and Hataihimakul, Sudkanung and Poghossian, Arshak and Baldsiefen, Gerhard and Busch, Heinz and Laube, Norbert and Kleinen, Lisa and Sch{\"o}ning, Michael Josef}, title = {Field-effect calcium sensor for the determination of the risk of urinary stone formation}, series = {Sensors and Actuators B: Chemical. 144 (2010), H. 2}, journal = {Sensors and Actuators B: Chemical. 144 (2010), H. 2}, pages = {374 -- 379}, year = {2010}, language = {en} } @article{BuniatyanHuckPoghossianetal.2013, author = {Buniatyan, V. and Huck, Christina and Poghossian, Arshak and Aroutiounian, V. M. and Sch{\"o}ning, Michael Josef}, title = {BaxSr1-x TiO3/pc-Si heterojunction}, series = {Armenian journal of physics}, volume = {6}, journal = {Armenian journal of physics}, number = {4}, publisher = {National Academy of Sciences of Armenia}, address = {Yerevan}, issn = {1829-1171}, pages = {177 -- 187}, year = {2013}, language = {en} } @article{YoshinobuEckenPoghossianetal.2001, author = {Yoshinobu, T. and Ecken, H. and Poghossian, Arshak and L{\"u}th, H. and Iwasaki, H. and Sch{\"o}ning, Michael Josef}, title = {Alternative sensor materials for light-addressable potentiometric sensors}, series = {Sensors and Actuators B. 76 (2001), H. 1-3}, journal = {Sensors and Actuators B. 76 (2001), H. 1-3}, isbn = {0925-4005}, pages = {388 -- 392}, year = {2001}, language = {en} } @article{PoghossianAbouzarSakkarietal.2006, author = {Poghossian, Arshak and Abouzar, Maryam H. and Sakkari, M. and Kassab, T. and Han, Y. and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Field-effect sensors for monitoring the layer-by-layer adsorption of charged macromolecules}, series = {Sensors and Actuators B: Chemical. 118 (2006), H. 1-2}, journal = {Sensors and Actuators B: Chemical. 118 (2006), H. 1-2}, isbn = {0925-4005}, pages = {163 -- 170}, year = {2006}, language = {en} } @article{KraemerPitaZhouetal.2009, author = {Kr{\"a}mer, Melina and Pita, Marcos and Zhou, Jian and Ornatska, Maryna and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Katz, Evgeny}, title = {Coupling of Biocomputing Systems with Electronic Chips: Electronic Interface for Transduction of Biochemical Information}, series = {Journal of Physical Chemistry C: Nanomaterials and Interfaces. 113 (2009), H. 6}, journal = {Journal of Physical Chemistry C: Nanomaterials and Interfaces. 113 (2009), H. 6}, publisher = {American Cemical Society}, address = {Washington, DC}, isbn = {1932-7455}, pages = {2573 -- 2579}, year = {2009}, language = {en} } @article{HuckJollyWagneretal.2011, author = {Huck, Christina and Jolly, Christina and Wagner, Patrick and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {One-chip integrated dual amperometric/field-effect sensor for the detection of dissolved hydrogen}, series = {Procedia Engineering. 25 (2011)}, journal = {Procedia Engineering. 25 (2011)}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1877-7058}, pages = {1161 -- 1164}, year = {2011}, language = {en} } @article{WuBronderPoghossianetal.2014, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Werner, Frederik and B{\"a}cker, Matthias and Sch{\"o}ning, Michael Josef}, title = {Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips}, series = {Physica status solidi A : Applications and materials science}, volume = {211}, journal = {Physica status solidi A : Applications and materials science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330442}, pages = {1423 -- 1428}, year = {2014}, abstract = {A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent-voltage (I-V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I-V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge.}, language = {en} } @inproceedings{NaetherPoghossianPlatenetal.2006, author = {N{\"a}ther, Niko and Poghossian, Arshak and Platen, J. and Yoshinobu, T. and Koudelka-Hep, M. and Sch{\"o}ning, Michael Josef}, title = {Multi-parameter sensing of both physical and (bio-)chemical quantities using the same transducer principle}, series = {Biochemical sensing utilisation of micro- and nanotechnologies : Warsaw, [23rd - 26th] November 2005 / ed. by M. Mascini ...}, booktitle = {Biochemical sensing utilisation of micro- and nanotechnologies : Warsaw, [23rd - 26th] November 2005 / ed. by M. Mascini ...}, address = {Warsaw}, pages = {172 -- 181}, year = {2006}, language = {en} } @article{PoghossianJablonskiMolinnusetal.2020, author = {Poghossian, Arshak and Jablonski, Melanie and Molinnus, Denise and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers}, series = {Frontiers in Plant Science}, volume = {11}, journal = {Frontiers in Plant Science}, number = {Article 598103}, publisher = {Frontiers}, address = {Lausanne}, doi = {10.3389/fpls.2020.598103}, pages = {1 -- 14}, year = {2020}, abstract = {Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.}, language = {en} } @article{SchusserLeinhosBaeckeretal.2013, author = {Schusser, Sebastian and Leinhos, Marcel and B{\"a}cker, Matthias and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Impedance spectroscopy: A tool for real-time in situ monitoring of the degradation of biopolymers}, series = {Physica Status Solidi (A)}, volume = {210}, journal = {Physica Status Solidi (A)}, number = {5}, publisher = {Wiley}, address = {Weinheim}, issn = {1521-396X ; 0031-8965}, doi = {10.1002/pssa.201200941}, pages = {905 -- 910}, year = {2013}, abstract = {Investigation of the degradation kinetics of biodegradable polymers is essential for the development of implantable biomedical devices with predicted biodegradability. In this work, an impedimetric sensor has been applied for real-time and in situ monitoring of degradation processes of biopolymers. The sensor consists of two platinum thin-film electrodes covered by a polymer film to be studied. The benchmark biomedical polymer poly(D,L-lactic acid) (PDLLA) was used as a model system. PDLLA films were deposited on the sensor structure from a polymer solution by using the spin-coating method. The degradation kinetics of PDLLA films have been studied in alkaline solutions of pH 9 and 12 by means of an impedance spectroscopy (IS) method. Any changes in a polymer capacitance/resistance induced by water uptake and/or polymer degradation will modulate the global impedance of the polymer-covered sensor that can be used as an indicator of the polymer degradation. The degradation rate can be evaluated from the time-dependent impedance spectra. As expected, a faster degradation has been observed for PDLLA films exposed to pH 12 solution.}, language = {en} } @article{PoghossianBerndsenLuethetal.2002, author = {Poghossian, Arshak and Berndsen, L. and L{\"u}th, H. and Schultze, J. W. and Sch{\"o}ning, Michael Josef}, title = {Multi-Parameter-Detektions-System unter dem Einsatz eines einzigen Transducerprinzips sowohl f{\"u}r (bio-)chemische als auch physikalische Sensoren}, series = {Sensoren und Mess-Systeme : Vortr{\"a}ge der 11. ITG/GMA-Fachtagung vom 11. bis 12. M{\"a}rz 2002 in Ludwigsburg / Veranst.: Informationstechnische Gesellschaft im VDE (ITG) ... Wiss. Tagungsleitung: C. D. Kohl ....}, journal = {Sensoren und Mess-Systeme : Vortr{\"a}ge der 11. ITG/GMA-Fachtagung vom 11. bis 12. M{\"a}rz 2002 in Ludwigsburg / Veranst.: Informationstechnische Gesellschaft im VDE (ITG) ... Wiss. Tagungsleitung: C. D. Kohl ....}, publisher = {VDE-Verl.}, address = {Berlin [u.a.]}, isbn = {3-8007-2675-0}, pages = {137 -- 140}, year = {2002}, language = {de} } @article{BaeckerPoghossianSchoeningetal.2010, author = {B{\"a}cker, Matthias and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Schnitzler, Thomas and Biselli, Manfred and Zang, Werner and Wagner, P.}, title = {Entwicklung eines modularen festk{\"o}rperbasierten Sensorsystems f{\"u}r die {\"U}berwachung von Zellkulturfermentationen}, series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, journal = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {688 -- 698}, year = {2010}, language = {de} } @book{SchoeningPoghossian2018, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Label-free biosensing: advanced materials, devices and applications}, publisher = {Springer}, address = {Cham}, isbn = {978-3-319-75219-8}, pages = {xii, 480 Seiten ; Illustrationen, Diagramme}, year = {2018}, language = {en} } @article{KarschuckPoghossianSeretal.2024, author = {Karschuck, Tobias and Poghossian, Arshak and Ser, Joey and Tsokolakyan, Astghik and Achtsnicht, Stefan and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Capacitive model of enzyme-modified field-effect biosensors: Impact of enzyme coverage}, series = {Sensors and Actuators B: Chemical}, volume = {408}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005 (Print)}, doi = {10.1016/j.snb.2024.135530}, pages = {12 Seiten}, year = {2024}, abstract = {Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.}, language = {en} } @article{OezsoyluKizildagSchoeningetal.2020, author = {{\"O}zsoylu, Dua and Kizildag, Sefa and Sch{\"o}ning, Michael Josef and Wagner, Torsten}, title = {Differential chemical imaging of extracellular acidification within microfluidic channels using a plasma-functionalized light-addressable potentiometric sensor (LAPS)}, series = {Physics in Medicine}, volume = {10}, journal = {Physics in Medicine}, number = {100030}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2352-4510}, doi = {10.1016/j.phmed.2020.100030}, pages = {8}, year = {2020}, abstract = {Extracellular acidification is a basic indicator for alterations in two vital metabolic pathways: glycolysis and cellular respiration. Measuring these alterations by monitoring extracellular acidification using cell-based biosensors such as LAPS plays an important role in studying these pathways whose disorders are associated with numerous diseases including cancer. However, the surface of the biosensors must be specially tailored to ensure high cell compatibility so that cells can represent more in vivo-like behavior, which is critical to gain more realistic in vitro results from the analyses, e.g., drug discovery experiments. In this work, O2 plasma patterning on the LAPS surface is studied to enhance surface features of the sensor chip, e.g., wettability and biofunctionality. The surface treated with O2 plasma for 30 s exhibits enhanced cytocompatibility for adherent CHO-K1 cells, which promotes cell spreading and proliferation. The plasma-modified LAPS chip is then integrated into a microfluidic system, which provides two identical channels to facilitate differential measurements of the extracellular acidification of CHO-K1 cells. To the best of our knowledge, it is the first time that extracellular acidification within microfluidic channels is quantitatively visualized as differential (bio-)chemical images.}, language = {en} } @article{AliaziziOezsoyluBakhshiSichanietal.2024, author = {Aliazizi, Fereshteh and {\"O}zsoylu, Dua and Bakhshi Sichani, Soroush and Khorshid, Mehran and Glorieux, Christ and Robbens, Johan and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Development and Calibration of a Microfluidic, Chip-Based Sensor System for Monitoring the Physical Properties of Water Samples in Aquacultures}, series = {Micromachines}, volume = {15}, journal = {Micromachines}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2072-666X}, doi = {10.3390/mi15060755}, year = {2024}, abstract = {In this work, we present a compact, bifunctional chip-based sensor setup that measures the temperature and electrical conductivity of water samples, including specimens from rivers and channels, aquaculture, and the Atlantic Ocean. For conductivity measurements, we utilize the impedance amplitude recorded via interdigitated electrode structures at a single triggering frequency. The results are well in line with data obtained using a calibrated reference instrument. The new setup holds for conductivity values spanning almost two orders of magnitude (river versus ocean water) without the need for equivalent circuit modelling. Temperature measurements were performed in four-point geometry with an on-chip platinum RTD (resistance temperature detector) in the temperature range between 2 °C and 40 °C, showing no hysteresis effects between warming and cooling cycles. Although the meander was not shielded against the liquid, the temperature calibration provided equivalent results to low conductive Milli-Q and highly conductive ocean water. The sensor is therefore suitable for inline and online monitoring purposes in recirculating aquaculture systems.}, language = {en} } @inproceedings{KirchnerHenkelNaetheretal.2008, author = {Kirchner, Patrick and Henkel, H. and N{\"a}ther, Niko and Spelthahn, H. and Schneider, A. and Berger, J. and Kolstad, J. and Friedrich, P. and Sch{\"o}ning, Michael Josef}, title = {RFID-basiertes Sensorsystem zur Realisierung intelligenter Verpackungen f{\"u}r die Nahrungsmittelindustrie}, series = {KMU - innovativ: IKT 2008. CD-ROM : BMBF-Statustagung KMU - innovativ: IKT, Darmstadt, 17. - 18. Nov. 2008}, booktitle = {KMU - innovativ: IKT 2008. CD-ROM : BMBF-Statustagung KMU - innovativ: IKT, Darmstadt, 17. - 18. Nov. 2008}, number = {CD-ROM-Ausg.}, publisher = {BMBF}, address = {Berlin}, pages = {9 S.}, year = {2008}, language = {de} } @article{KarschuckKaulenPoghossianetal.2021, author = {Karschuck, Tobias and Kaulen, Corinna and Poghossian, Arshak and Wagner, Patrick H. and Sch{\"o}ning, Michael Josef}, title = {Gold nanoparticle-modified capacitive field-effect sensors: Studying the surface density of nanoparticles and coupling of charged polyelectrolyte macromolecules}, series = {Electrochemical Science Advances}, volume = {2}, journal = {Electrochemical Science Advances}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0938-5193}, doi = {10.1002/elsa.202100179}, pages = {10 Seiten}, year = {2021}, abstract = {The coupling of ligand-stabilized gold nanoparticles with field-effect devices offers new possibilities for label-free biosensing. In this work, we study the immobilization of aminooctanethiol-stabilized gold nanoparticles (AuAOTs) on the silicon dioxide surface of a capacitive field-effect sensor. The terminal amino group of the AuAOT is well suited for the functionalization with biomolecules. The attachment of the positively-charged AuAOTs on a capacitive field-effect sensor was detected by direct electrical readout using capacitance-voltage and constant capacitance measurements. With a higher particle density on the sensor surface, the measured signal change was correspondingly more pronounced. The results demonstrate the ability of capacitive field-effect sensors for the non-destructive quantitative validation of nanoparticle immobilization. In addition, the electrostatic binding of the polyanion polystyrene sulfonate to the AuAOT-modified sensor surface was studied as a model system for the label-free detection of charged macromolecules. Most likely, this approach can be transferred to the label-free detection of other charged molecules such as enzymes or antibodies.}, language = {en} } @article{PoghossianWeldenBuniatyanetal.2021, author = {Poghossian, Arshak and Welden, Rene and Buniatyan, Vahe V. and Sch{\"o}ning, Michael Josef}, title = {An Array of On-Chip Integrated, Individually Addressable Capacitive Field-Effect Sensors with Control Gate: Design and Modelling}, series = {Sensors}, volume = {21}, journal = {Sensors}, number = {18}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s21186161}, pages = {17}, year = {2021}, abstract = {The on-chip integration of multiple biochemical sensors based on field-effect electrolyte-insulator-semiconductor capacitors (EISCAP) is challenging due to technological difficulties in realization of electrically isolated EISCAPs on the same Si chip. In this work, we present a new simple design for an array of on-chip integrated, individually electrically addressable EISCAPs with an additional control gate (CG-EISCAP). The existence of the CG enables an addressable activation or deactivation of on-chip integrated individual CG-EISCAPs by simple electrical switching the CG of each sensor in various setups, and makes the new design capable for multianalyte detection without cross-talk effects between the sensors in the array. The new designed CG-EISCAP chip was modelled in so-called floating/short-circuited and floating/capacitively-coupled setups, and the corresponding electrical equivalent circuits were developed. In addition, the capacitance-voltage curves of the CG-EISCAP chip in different setups were simulated and compared with that of a single EISCAP sensor. Moreover, the sensitivity of the CG-EISCAP chip to surface potential changes induced by biochemical reactions was simulated and an impact of different parameters, such as gate voltage, insulator thickness and doping concentration in Si, on the sensitivity has been discussed.}, language = {en} } @article{VahidpourGuthmanArreolaetal.2022, author = {Vahidpour, Farnoosh and Guthman, Eric and Arreola, Julia and Alghazali, Yousef H. M. and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Assessment of Various Process Parameters for Optimized Sterilization Conditions Using a Multi-Sensing Platform}, series = {Foods}, volume = {11}, journal = {Foods}, number = {5}, publisher = {MDPI}, address = {Basel}, issn = {2304-8158}, doi = {10.3390/foods11050660}, pages = {Artikel 660}, year = {2022}, abstract = {In this study, an online multi-sensing platform was engineered to simultaneously evaluate various process parameters of food package sterilization using gaseous hydrogen peroxide (H₂O₂). The platform enabled the validation of critical aseptic parameters. In parallel, one series of microbiological count reduction tests was performed using highly resistant spores of B. atrophaeus DSM 675 to act as the reference method for sterility validation. By means of the multi-sensing platform together with microbiological tests, we examined sterilization process parameters to define the most effective conditions with regards to the highest spore kill rate necessary for aseptic packaging. As these parameters are mutually associated, a correlation between different factors was elaborated. The resulting correlation indicated the need for specific conditions regarding the applied H₂O₂ gas temperature, the gas flow and concentration, the relative humidity and the exposure time. Finally, the novel multi-sensing platform together with the mobile electronic readout setup allowed for the online and on-site monitoring of the sterilization process, selecting the best conditions for sterility and, at the same time, reducing the use of the time-consuming and costly microbiological tests that are currently used in the food package industry.}, language = {en} } @article{PoghossianKarschuckWagneretal.2022, author = {Poghossian, Arshak and Karschuck, Tobias and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Field-Effect Capacitors Decorated with Ligand-Stabilized Gold Nanoparticles: Modeling and Experiments}, series = {Biosensors}, volume = {12}, journal = {Biosensors}, number = {5}, publisher = {MDPI}, address = {Basel}, issn = {2079-6374}, doi = {10.3390/bios12050334}, pages = {Artikel 334}, year = {2022}, abstract = {Nanoparticles are recognized as highly attractive tunable materials for designing field-effect biosensors with enhanced performance. In this work, we present a theoretical model for electrolyte-insulator-semiconductor capacitors (EISCAP) decorated with ligand-stabilized charged gold nanoparticles. The charged AuNPs are taken into account as additional, nanometer-sized local gates. The capacitance-voltage (C-V) curves and constant-capacitance (ConCap) signals of the AuNP-decorated EISCAPs have been simulated. The impact of the AuNP coverage on the shift of the C-V curves and the ConCap signals was also studied experimentally on Al-p-Si-SiO₂ EISCAPs decorated with positively charged aminooctanethiol-capped AuNPs. In addition, the surface of the EISCAPs, modified with AuNPs, was characterized by scanning electron microscopy for different immobilization times of the nanoparticles.}, language = {en} } @article{OezsoyluKizildagSchoeningetal.2019, author = {{\"O}zsoylu, Dua and Kizildag, Sefa and Sch{\"o}ning, Michael Josef and Wagner, Torsten}, title = {Effect of plasma treatment on the sensor properties of a light-addressable potentiometric sensor (LAPS)}, series = {physica status solidi a : applications and materials sciences}, volume = {216}, journal = {physica status solidi a : applications and materials sciences}, number = {20}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201900259}, pages = {8 Seiten}, year = {2019}, abstract = {A light-addressable potentiometric sensor (LAPS) is a field-effect-based (bio-) chemical sensor, in which a desired sensing area on the sensor surface can be defined by illumination. Light addressability can be used to visualize the concentration and spatial distribution of the target molecules, e.g., H+ ions. This unique feature has great potential for the label-free imaging of the metabolic activity of living organisms. The cultivation of those organisms needs specially tailored surface properties of the sensor. O2 plasma treatment is an attractive and promising tool for rapid surface engineering. However, the potential impacts of the technique are carefully investigated for the sensors that suffer from plasma-induced damage. Herein, a LAPS with a Ta2O5 pH-sensitive surface is successfully patterned by plasma treatment, and its effects are investigated by contact angle and scanning LAPS measurements. The plasma duration of 30 s (30 W) is found to be the threshold value, where excessive wettability begins. Furthermore, this treatment approach causes moderate plasma-induced damage, which can be reduced by thermal annealing (10 min at 300 °C). These findings provide a useful guideline to support future studies, where the LAPS surface is desired to be more hydrophilic by O2 plasma treatment.}, language = {en} } @article{KarschuckSchmidtAchtsnichtetal.2023, author = {Karschuck, Tobias and Schmidt, Stefan and Achtsnicht, Stefan and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Multiplexing system for automated characterization of a capacitive field-effect sensor array}, series = {Physica Status Solidi A}, volume = {220}, journal = {Physica Status Solidi A}, number = {22}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300 (Print)}, doi = {10.1002/pssa.202300265}, pages = {7 Seiten}, year = {2023}, abstract = {In comparison to single-analyte devices, multiplexed systems for a multianalyte detection offer a reduced assay time and sample volume, low cost, and high throughput. Herein, a multiplexing platform for an automated quasi-simultaneous characterization of multiple (up to 16) capacitive field-effect sensors by the capacitive-voltage (C-V) and the constant-capacitance (ConCap) mode is presented. The sensors are mounted in a newly designed multicell arrangement with one common reference electrode and are electrically connected to the impedance analyzer via the base station. A Python script for the automated characterization of the sensors executes the user-defined measurement protocol. The developed multiplexing system is tested for pH measurements and the label-free detection of ligand-stabilized, charged gold nanoparticles.}, language = {en} } @article{WendlandtKochBritzetal.2023, author = {Wendlandt, Tim and Koch, Claudia and Britz, Beate and Liedek, Anke and Schmidt, Nora and Werner, Stefan and Gleba, Yuri and Vahidpour, Farnoosh and Welden, Melanie and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System}, series = {Viruses}, volume = {9}, journal = {Viruses}, number = {15}, publisher = {MDPI}, address = {Basel}, issn = {1999-4915}, doi = {doi.org/10.3390/v15091951}, pages = {Artikel 1951}, year = {2023}, abstract = {Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.}, language = {en} } @article{YoshinobuMiyamotoWagneretal.2024, author = {Yoshinobu, Tatsuo and Miyamoto, Ko-ichiro and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Field-effect sensors combined with the scanned light pulse technique: from artificial olfactory images to chemical imaging technologies}, series = {Chemosensors}, volume = {12}, journal = {Chemosensors}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors12020020}, pages = {Artikel 20}, year = {2024}, abstract = {The artificial olfactory image was proposed by Lundstr{\"o}m et al. in 1991 as a new strategy for an electronic nose system which generated a two-dimensional mapping to be interpreted as a fingerprint of the detected gas species. The potential distribution generated by the catalytic metals integrated into a semiconductor field-effect structure was read as a photocurrent signal generated by scanning light pulses. The impact of the proposed technology spread beyond gas sensing, inspiring the development of various imaging modalities based on the light addressing of field-effect structures to obtain spatial maps of pH distribution, ions, molecules, and impedance, and these modalities have been applied in both biological and non-biological systems. These light-addressing technologies have been further developed to realize the position control of a faradaic current on the electrode surface for localized electrochemical reactions and amperometric measurements, as well as the actuation of liquids in microfluidic devices.}, language = {en} } @article{BertzMolinnusSchoeningetal.2023, author = {Bertz, Morten and Molinnus, Denise and Sch{\"o}ning, Michael Josef and Homma, Takayuki}, title = {Real-time monitoring of H₂O₂ sterilization on individual bacillus atrophaeus spores by optical sensing with trapping Raman spectroscopy}, series = {Chemosensors}, volume = {8}, journal = {Chemosensors}, number = {11}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors11080445}, pages = {Artikel 445}, year = {2023}, abstract = {Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore's core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores' coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death.}, language = {en} } @inproceedings{MorenoDosevBratovetal.2006, author = {Moreno, Lia and Dosev, D. and Bratov, A. and Dominguez, C. and Sch{\"o}ning, Michael Josef and Kloock, Joachim P.}, title = {Effect of electrical properties of the surrounding medium on the response of an interdigitated electrode array with chalcogenide glass film}, series = {XX Eurosensors : 20th anniversary ; G{\"o}teborg, Sweden, 17 - 20 September 2006 ; [proceedings]. - Vol. 1}, booktitle = {XX Eurosensors : 20th anniversary ; G{\"o}teborg, Sweden, 17 - 20 September 2006 ; [proceedings]. - Vol. 1}, address = {G{\"o}teborg}, isbn = {978-91-631-9280-7}, pages = {384 -- 385}, year = {2006}, language = {en} } @article{AridaAlHaddadSchoening2011, author = {Arida, Hassan A. and Al-Haddad, Ameera and Sch{\"o}ning, Michael Josef}, title = {New Solid-State Organic Membrane Based Lead-Selective Micro-Electrode}, series = {International Journal of Electrochemical Science. 6 (2011), H. 9}, journal = {International Journal of Electrochemical Science. 6 (2011), H. 9}, isbn = {1452-3981}, pages = {3858 -- 3867}, year = {2011}, language = {en} } @article{PoghossianJablonskiKochetal.2018, author = {Poghossian, Arshak and Jablonski, Melanie and Koch, Claudia and Bronder, Thomas and Rolka, David and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Field-effect biosensor using virus particles as scaffolds for enzyme immobilization}, series = {Biosensors and Bioelectronics}, volume = {110}, journal = {Biosensors and Bioelectronics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0956-5663}, doi = {10.1016/j.bios.2018.03.036}, pages = {168 -- 174}, year = {2018}, abstract = {A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples.}, language = {en} } @article{BaeckerSchusserPoghossianetal.2014, author = {B{\"a}cker, Matthias and Schusser, Sebastian and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Multi-Parametererfassung mit siliziumbasiertem Sensorchip: Aus Drei mach Eins}, series = {GIT Labor-Fachzeitschrift}, journal = {GIT Labor-Fachzeitschrift}, number = {2}, publisher = {Wiley}, issn = {0016-3538}, pages = {28 -- 30}, year = {2014}, language = {de} } @article{SchoeningPoghossianSchultzeetal.2002, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Schultze, J. W. and L{\"u}th, H.}, title = {Field-effect based multifunctional hybrid sensor module for the determination of both (bio-)chemical and physical paramters}, series = {Proceedings of SPIE. 4576 (2002)}, journal = {Proceedings of SPIE. 4576 (2002)}, pages = {149 -- 159}, year = {2002}, language = {en} } @article{PoghossianBerndsenSchoening2002, author = {Poghossian, Arshak and Berndsen, L. and Sch{\"o}ning, Michael Josef}, title = {Chemical sensor as a physical sensor: ISFET-based flowvelocity, flow-direction and diffusion-coefficient sensor}, series = {Book of abstracts / ed. by J. Saneistr.}, journal = {Book of abstracts / ed. by J. Saneistr.}, publisher = {Czech Technical University, Faculty of Electrical Engineering, Department of Measurement}, address = {Prague}, isbn = {80-01-02576-4}, pages = {649 -- 652}, year = {2002}, language = {en} } @article{NaetherAugerPoghossianetal.2004, author = {N{\"a}ther, Niko and Auger, V. and Poghossian, Arshak and Koudelka-Hep, M. and Sch{\"o}ning, Michael Josef}, title = {A miniaturized flow-through cell in SU-8 technique for EIS sensors}, series = {Biomedizinische Technik. 49 (2004), H. 2}, journal = {Biomedizinische Technik. 49 (2004), H. 2}, isbn = {0932-4666}, pages = {994 -- 995}, year = {2004}, language = {en} } @incollection{PoghossianWeilandSchoening2014, author = {Poghossian, Arshak and Weiland, Maryam and Sch{\"o}ning, Michael Josef}, title = {Nanoplate field-effect capacitors: a new transducer structure for multiparameter (bio-)chemical sensing}, series = {Multisensor system for chemical analysis : materials and sensors}, booktitle = {Multisensor system for chemical analysis : materials and sensors}, editor = {Lvova, Larisa and Kirsanov, Dmitry and di Natale, Corrado and Legin, Audrey}, edition = {1}, publisher = {Jenny Stanford Publishing}, address = {Singapore}, isbn = {978-981-4411-15-8 ; 978-981-4411-16-5}, doi = {10.1201/b15491-11}, pages = {333 -- 373}, year = {2014}, abstract = {An array of electrically isolated nanoplate field-effect silicon-on-insulator (SOI) capacitors as a new transducer structure for multiparameter (bio-)chemical sensing is presented. The proposed approach allows addressable biasing and electrical readout of multiple nanoplate field-effect capacitive (bio-)chemical sensors on the same SOI chip, as well as differential-mode measurements. The realized sensor chip has been applied for pH and penicillin concentration measurements, electrical monitoring of polyelectrolyte multilayer formation, and the label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization and denaturation events.}, language = {en} } @article{BuniatyanHuckPoghossianetal.2013, author = {Buniatyan, V. and Huck, Christina and Poghossian, Arshak and Aroutiounian, V. M. and Sch{\"o}ning, Michael Josef}, title = {BaxSr1-x TiO3/pc-Si heterojunction capacitance}, series = {Armenian journal of physics}, volume = {6}, journal = {Armenian journal of physics}, number = {4}, publisher = {National Academy of Sciences of Armenia}, address = {Yerevan}, issn = {1829-1171}, pages = {188 -- 197}, year = {2013}, language = {en} } @article{SchoeningPoghossianSchultze2003, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Schultze, Joachim W.}, title = {Measuring seven parameters by two ISFET modules in a microcell set-up}, series = {Int. Journal of Computational Engineering Science. 4 (2003), H. 2}, journal = {Int. Journal of Computational Engineering Science. 4 (2003), H. 2}, isbn = {1465-8763}, pages = {257 -- 260}, year = {2003}, language = {en} } @inproceedings{PoghossianAbouzarSchoening2012, author = {Poghossian, Arshak and Abouzar, Maryam H. and Sch{\"o}ning, Michael Josef}, title = {(Bio-­)chemical sensor array based on nanoplate SOI capacitors}, series = {Nanoscale Science and Technology (NS\&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012}, booktitle = {Nanoscale Science and Technology (NS\&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012}, editor = {Abdelghani, Adnane and Sch{\"o}ning, Michael Josef}, pages = {31 -- 31}, year = {2012}, language = {en} } @article{HuckPoghossianBaeckeretal.2015, author = {Huck, Christina and Poghossian, Arshak and B{\"a}cker, Matthias and Reisert, Steffen and Kramer, Friederike and Begoyan, Vardges K. and Buniatyan, Vahe V. and Sch{\"o}ning, Michael Josef}, title = {Multi-parameter sensing using high-k oxide of barium strontium titanate}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431911}, pages = {1259}, year = {2015}, abstract = {High-k perovskite oxide of barium strontium titanate (BST) represents a very attractive multi-functional transducer material for the development of (bio-)chemical sensors. In this work, a Si-based sensor chip containing Pt interdigitated electrodes covered with a thin BST layer (485 nm) has been developed for multi-parameter chemical sensing. The chip has been applied for the contactless measurement of the electrolyte conductivity, the detection of adsorbed charged macromolecules (positively charged polyelectrolytes of polyethylenimine) and the concentration of hydrogen peroxide (H2O2) vapor. The experimental results of functional testing of individual sensors are presented. The mechanism of the BST sensitivity to charged polyelectrolytes and H2O2 vapor has been proposed and discussed.}, language = {en} } @inproceedings{SchusserLeinhosPoghossianetal.2012, author = {Schusser, Sebastian and Leinhos, Marcel and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Biopolymer-degradation monitoring by chip-­based impedance spectroscopy technique}, series = {Nanoscale Science and Technology (NS\&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012}, booktitle = {Nanoscale Science and Technology (NS\&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012}, editor = {Abdelghani, Adnane and Sch{\"o}ning, Michael Josef}, pages = {47 -- 47}, year = {2012}, language = {en} } @article{IkenAhlbornGerlachetal.2013, author = {Iken, Heiko and Ahlborn, Kristina and Gerlach, Frank and Vonau, Winfried and Zander, Wilhelm and Schubert, J{\"u}rgen P. and Sch{\"o}ning, Michael Josef}, title = {Development of redox glasses and subsequent processing by means of pulsed laser deposition for realizing silicon-based thin-film sensors}, series = {Electrochimica acta}, journal = {Electrochimica acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-3859 (E-Journal); 0013-4686 (Print)}, pages = {Available online 30.8.2013}, year = {2013}, language = {en} } @article{VahidpourAlghazaliAkcaetal.2022, author = {Vahidpour, Farnoosh and Alghazali, Yousef H. M. and Akca, Sevilay and Hommes, Gregor and Sch{\"o}ning, Michael Josef}, title = {An Enzyme-Based Interdigitated Electrode-Type Biosensor for Detecting Low Concentrations of H₂O₂ Vapor/Aerosol}, series = {Chemosensors}, volume = {10}, journal = {Chemosensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors10060202}, pages = {Arikel 202}, year = {2022}, abstract = {This work introduces a novel method for the detection of H₂O₂ vapor/aerosol of low concentrations, which is mainly applied in the sterilization of equipment in medical industry. Interdigitated electrode (IDE) structures have been fabricated by means of microfabrication techniques. A differential setup of IDEs was prepared, containing an active sensor element (active IDE) and a passive sensor element (passive IDE), where the former was immobilized with an enzymatic membrane of horseradish peroxidase that is selective towards H₂O₂. Changes in the IDEs' capacitance values (active sensor element versus passive sensor element) under H₂O₂ vapor/aerosol atmosphere proved the detection in the concentration range up to 630 ppm with a fast response time (<60 s). The influence of relative humidity was also tested with regard to the sensor signal, showing no cross-sensitivity. The repeatability assessment of the IDE biosensors confirmed their stable capacitive signal in eight subsequent cycles of exposure to H₂O₂ vapor/aerosol. Room-temperature detection of H₂O₂ vapor/aerosol with such miniaturized biosensors will allow a future three-dimensional, flexible mapping of aseptic chambers and help to evaluate sterilization assurance in medical industry.}, language = {en} } @article{GrinsvenVandenBonGrietenetal.2011, author = {Grinsven, Bart van and Vanden Bon, Natalie and Grieten, Lars and Murib, Mohammed Sharif and Janssen, Stoffel Dominique and Haenen, Ken and Schneider, E. and Ingebrandt, Sven and Sch{\"o}ning, Michael Josef and Vermeeren, Veronique and Ameloot, Marcel and Michiels, Luc and Thoelen, Ronald and De Ceuninck, Ward A. and Wagner, Patrick}, title = {Rapid assessment of the stability of DNA duplexes by impedimetric real-time monitoring of chemically induced denaturation}, series = {Lab on a Chip}, volume = {11}, journal = {Lab on a Chip}, number = {9}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, isbn = {1473-0197}, pages = {1656 -- 1663}, year = {2011}, language = {en} } @article{MuribGrinsvenGrietenetal.2013, author = {Murib, M. S. and Grinsven, B. van and Grieten, L. and Janssens, S. D. and Vermeeren, V. and Eersels, K. and Broeders, J. and Ameloot, Marcel and Michiels, L. and Ceuninck, W. De and Haenen, K. and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Electronic monitoring of chemical DNA denaturation on nanocrystalline diamond electrodes with different molarities and flow rates}, series = {Physica Status Solidi (A). Vol. 210 (2013), iss. 5}, journal = {Physica Status Solidi (A). Vol. 210 (2013), iss. 5}, publisher = {Wiley-VCH}, address = {Berlin}, issn = {0031-8965}, pages = {911 -- 917}, year = {2013}, language = {en} } @article{MuribYeapEurlingsetal.2016, author = {Murib, M. S. and Yeap, W. S. and Eurlings, Y. and Grinsven, B. van and Boyen, H.-G. and Conings, B. and Michiels, L. and Ameloot, Marcel and Carleer, R. and Warmer, J. and Kaul, P. and Haenen, K. and Sch{\"o}ning, Michael Josef and Ceuninck, W. de and Wagner, P.}, title = {Heat-transfer based characterization of DNA on synthetic sapphire chips}, series = {Sensors and Actuators B: Chemical}, volume = {230}, journal = {Sensors and Actuators B: Chemical}, number = {230}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.02.027}, pages = {260 -- 271}, year = {2016}, abstract = {In this study, we show that synthetic sapphire (Al₂O₃), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material.}, language = {en} }