@article{VoegeleRuebbelkeGovorukhaetal.2019, author = {V{\"o}gele, Stefan and R{\"u}bbelke, Dirk and Govorukha, Kristina and Grajewski, Matthias}, title = {Socio-technical scenarios for energy-intensive industries: the future of steel production in Germany}, series = {Climatic Change}, journal = {Climatic Change}, publisher = {Springer}, address = {Berlin}, issn = {0165-0009}, doi = {10.1007/s10584-019-02366-0}, pages = {1 -- 16}, year = {2019}, language = {en} } @article{ThomaRavi2019, author = {Thoma, Andreas and Ravi, Sridhar}, title = {Significance of parallel computing on the performance of Digital Image Correlation algorithms in MATLAB}, pages = {1 -- 17}, year = {2019}, abstract = {Digital Image Correlation (DIC) is a powerful tool used to evaluate displacements and deformations in a non-intrusive manner. By comparing two images, one of the undeformed reference state of a specimen and another of the deformed target state, the relative displacement between those two states is determined. DIC is well known and often used for post-processing analysis of in-plane displacements and deformation of specimen. Increasing the analysis speed to enable real-time DIC analysis will be beneficial and extend the field of use of this technique. Here we tested several combinations of the most common DIC methods in combination with different parallelization approaches in MATLAB and evaluated their performance to determine whether real-time analysis is possible with these methods. To reflect improvements in computing technology different hardware settings were also analysed. We found that implementation problems can reduce the efficiency of a theoretically superior algorithm such that it becomes practically slower than a suboptimal algorithm. The Newton-Raphson algorithm in combination with a modified Particle Swarm algorithm in parallel image computation was found to be most effective. This is contrary to theory, suggesting that the inverse-compositional Gauss-Newton algorithm is superior. As expected, the Brute Force Search algorithm is the least effective method. We also found that the correct choice of parallelization tasks is crucial to achieve improvements in computing speed. A poorly chosen parallelisation approach with high parallel overhead leads to inferior performance. Finally, irrespective of the computing mode the correct choice of combinations of integerpixel and sub-pixel search algorithms is decisive for an efficient analysis. Using currently available hardware realtime analysis at high framerates remains an aspiration.}, language = {en} } @article{CornelisGivanoudiYongabietal.2019, author = {Cornelis, Peter and Givanoudi, Stella and Yongabi, Derick and Iken, Heiko and Duw{\´e}, Sam and Deschaume, Olivier and Robbens, Johan and Dedecker, Peter and Bartic, Carmen and W{\"u}bbenhorst, Michael and Sch{\"o}ning, Michael Josef and Heyndrickx, Marc and Wagner, Patrick}, title = {Sensitive and specific detection of E. coli using biomimetic receptors in combination with a modified heat-transfer method}, series = {Biosensors and Bioelectronics}, volume = {136}, journal = {Biosensors and Bioelectronics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0956-5663}, doi = {10.1016/j.bios.2019.04.026}, pages = {97 -- 105}, year = {2019}, language = {en} } @article{AchtsnichtNeuendorfFassbenderetal.2019, author = {Achtsnicht, Stefan and Neuendorf, Christian and Faßbender, Tobias and N{\"o}lke, Greta and Offenh{\"a}usser, Andreas and Krause, Hans-Joachim and Schr{\"o}per, Florian}, title = {Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection}, series = {Plos One}, volume = {14}, journal = {Plos One}, number = {7}, publisher = {Plos}, address = {San Francisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0219356}, pages = {e0219356}, year = {2019}, abstract = {Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin's B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more than 700 ng/ml.}, language = {en} } @article{KnoedlerRuehlEmontsetal.2019, author = {Kn{\"o}dler, Matthias and R{\"u}hl, Clemens and Emonts, Jessica and Buyel, Johannes Felix}, title = {Seasonal weather changes affect the yield and quality of recombinant proteins produced in transgenic tobacco plants in a greenhouse setting}, series = {Frontiers in Plant Science}, journal = {Frontiers in Plant Science}, number = {10}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-462X (online-ressource)}, doi = {10.3389/fpls.2019.01245}, pages = {13 Seiten}, year = {2019}, abstract = {Transgenic plants have the potential to produce recombinant proteins on an agricultural scale, with yields of several tons per year. The cost-effectiveness of transgenic plants increases if simple cultivation facilities such as greenhouses can be used for production. In such a setting, we expressed a novel affinity ligand based on the fluorescent protein DsRed, which we used as a carrier for the linear epitope ELDKWA from the HIV-neutralizing antibody 2F5. The DsRed-2F5-epitope (DFE) fusion protein was produced in 12 consecutive batches of transgenic tobacco (Nicotiana tabacum) plants over the course of 2 years and was purified using a combination of blanching and immobilized metal-ion affinity chromatography (IMAC). The average purity after IMAC was 57 ± 26\% (n = 24) in terms of total soluble protein, but the average yield of pure DFE (12 mg kg-1) showed substantial variation (± 97 mg kg-1, n = 24) which correlated with seasonal changes. Specifically, we found that temperature peaks (>28°C) and intense illuminance (>45 klx h-1) were associated with lower DFE yields after purification, reflecting the loss of the epitope-containing C-terminus in up to 90\% of the product. Whereas the weather factors were of limited use to predict product yields of individual harvests conducted for each batch (spaced by 1 week), the average batch yields were well approximated by simple linear regression models using two independent variables for prediction (illuminance and plant age). Interestingly, accumulation levels determined by fluorescence analysis were not affected by weather conditions but positively correlated with plant age, suggesting that the product was still expressed at high levels, but the extreme conditions affected its stability, albeit still preserving the fluorophore function. The efficient production of intact recombinant proteins in plants may therefore require adequate climate control and shading in greenhouses or even cultivation in fully controlled indoor farms.}, language = {en} } @article{PilasSelmerKeusgenetal.2019, author = {Pilas, Johanna and Selmer, Thorsten and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Screen-printed carbon electrodes modified with graphene oxide for the design of a reagent-free NAD+-dependent biosensor array}, series = {Analytical Chemistry}, volume = {91}, journal = {Analytical Chemistry}, number = {23}, publisher = {ACS Publications}, address = {Washington}, doi = {10.1021/acs.analchem.9b04481}, pages = {15293 -- 15299}, year = {2019}, language = {en} } @article{HueningHillgaertnerReke2019, author = {H{\"u}ning, Felix and Hillg{\"a}rtner, Michael and Reke, Michael}, title = {Rolling Labs - Teaching Vehicle Electronics from the Beginning}, series = {International Journal of Engineering Pedagogy (iJEP)}, volume = {9}, journal = {International Journal of Engineering Pedagogy (iJEP)}, number = {1}, issn = {2192-4880}, doi = {10.3991/ijep.v9i1.9241}, pages = {34 -- 49}, year = {2019}, language = {en} } @article{ValeroChansonBung2019, author = {Valero, Daniel and Chanson, Hubert and Bung, Daniel Bernhard}, title = {Robust estimators for turbulence properties assessment}, pages = {1 -- 24}, year = {2019}, language = {en} } @article{QuittmannAbelAlbrachtetal.2019, author = {Quittmann, Oliver J. and Abel, Thomas and Albracht, Kirsten and Str{\"u}der, Heiko K.}, title = {Reliability of muscular activation patterns and their alterations during incremental handcycling in able-bodied participants}, series = {Sports Biomechanics}, journal = {Sports Biomechanics}, number = {Article in press}, publisher = {Taylor \& Francis}, address = {London}, issn = {1752-6116}, doi = {10.1080/14763141.2019.1593496}, year = {2019}, language = {en} } @article{Golland2019, author = {Golland, Alexander}, title = {Reichweite des "Joint Controllership": Neue Fragen der gemeinsamen Verantwortlichkeit}, series = {K\&R Kommunikation \& Recht}, volume = {22}, journal = {K\&R Kommunikation \& Recht}, number = {9}, publisher = {dfv Mediengruppe, Deutscher Fachverlag GmbH}, address = {Frankfurt am Main}, issn = {1434-6354}, pages = {533 -- 537}, year = {2019}, abstract = {Kein Urteil zum Datenschutzrecht sorgte im vergangenen Jahr f{\"u}r mehr panische Reaktionen als die Entscheidung des EuGH in der Rechtssache "Wirtschaftsakademie Schleswig-Holstein"(C-210/16). Das Urteil warf in datenschutzrechtlicher Literatur und {\"O}ffentlichkeit zahlreiche Fragen auf: Ist jetzt jeder "gemeinsam" Verantwortlicher? Was sind die Kriterien? Der EuGH hat k{\"u}rzlich in einem - dem allgemeinen Vernehmen nach aufsehenerregenden, de facto aber kaum {\"u}berraschenden - Urteil f{\"u}r Klarheit gesorgt. Dabei hat das Gericht jedoch einige Fragen offengelassen und neue Fragen aufgeworfen. Ein Blick auf alte und neue Herausforderungen in Kooperationsszenarien.}, language = {de} }