@article{DegeringEggertPulsetal.2010,
  author    = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich},
  title     = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides},
  series = {Applied and environmental microbiology},
  volume    = {76},
  journal   = {Applied and environmental microbiology},
  number    = {19},
  publisher = {American Society for Microbiology},
  address   = {Washington, DC},
  issn      = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)},
  doi       = {10.1128/AEM.01146-10},
  pages     = {6370 -- 6378},
  year      = {2010},
  abstract  = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.},
  language  = {en}
}
@article{ReisgenSchleserMokrovetal.2010,
  author    = {Reisgen, Uwe and Schleser, Markus and Mokrov, Oleg and Ahmed, Essam},
  title     = {Uni- and bi-axial deformation behavior of laser welded advanced high strength steel sheets},
  series = {Journal of materials processing technology},
  volume    = {210},
  journal   = {Journal of materials processing technology},
  number    = {15},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0924-0136},
  doi       = {http10.1016/j.jmatprotec.2010.08.003},
  pages     = {2188 -- 2196},
  year      = {2010},
  abstract  = {Bead-on-plate butt joints of 2.5 mm hot rolled DP600/DP600 and 1.2 mm cold rolled TRIP700/TRIP700 steel sheets were performed using 6 kW CO2 laser beam welding. The welding speed ranged from 1.5 to 3.0 and from 2.1 to 3.9 m/min in DP/DP and TRIP/TRIP steel weldments respectively. A top surface helium gas was used as a shielding gas at a flow rate of 20 l/min. Metallographic examinations and transverse tensile testing (DIN EN 895: 1995) were carried out to characterize the weldments. The formability of base metals and weldments were investigated by standard Erichsen test (DIN EN ISO 20482). It was found that the uniaxial plastic behavior of both DP600 and TRIP700 base metals was in agreement with Swift and modified Mecking-Kocks models respectively. In a perpendicular tensile test to the weld line, all specimens were fractured at the base metal however the strengths were somewhat higher than those of base metal. There was a significant reduction in formability caused by welding of both DP/DP and TRIP/TRIP steel weldments and the formability has been improved with the increase of the welding speed.},
  language  = {en}
}
@article{ReisgenSchleserJerabeketal.2010,
  author    = {Reisgen, Uwe and Schleser, Markus and Jerabek, Jakub and Chudoba, Rostislav},
  title     = {Messung der Strahlqualit{\"a}t einer Elektronenstrahlanlage in Umgebungsatmosph{\"a}re},
  series = {Materialwissenschaft und Werkstofftechnik},
  volume    = {41},
  journal   = {Materialwissenschaft und Werkstofftechnik},
  number    = {1},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1521-4052},
  pages     = {45 -- 52},
  year      = {2010},
  abstract  = {In den letzten Jahrzehnten hat das Elektronenstrahlschweißen, das bereits im gr{\"o}ßeren Maßstab verwendet wird, seine F{\"a}higkeit als qualitatives Werkzeug f{\"u}r die Verbindung verschiedener Materialen nachgewiesen. Das Non Vacuum Electron Beam Welding (NV-EBW) hat zahlreiche Vorteile im Vergleich zum Elektronenstrahlschweißen im Vakuum, da man unter normalem Atmosph{\"a}rendruck arbeiten kann. Im Hinblick auf die reproduzierbare Qualit{\"a}t, insbesondere im Bereich der Massen-Fertigung, ist die Kontrolle der Strahlparameter sowie deren Einfluss auf das Schweißergebnis von großer Bedeutung. Durch eine genaue Kenntnis der Strahlkenngr{\"o}ßen wie des Strahldurchmessers und der Leistungsdichteverteilung kann eine Aussage {\"u}ber die sich ausbildende Schweißnaht sowie die Schweißbaddynamik getroffen werden. Messungen der Strahlkenngr{\"o}ßen im Prozess erlauben insbesondere die Untersuchung von Humping-Effekten. In diesem Beitrag wird der Prozess der Elektronenstrahlvermessung unter atmosph{\"a}rischen Bedingungen beschrieben. Es wird zudem die Abh{\"a}ngigkeit der Elektronenstrahlcharakteristika von den verschiedenen Prozessparametern dargestellt.},
  language  = {de}
}
@article{KuhnhenneDoeringFeldmann2010,
  author    = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Feldmann, Markus},
  title     = {Grunds{\"a}tze und L{\"o}sungen zur W{\"a}rmebr{\"u}ckenreduktion im Metallleichtbau},
  series = {Stahlbau},
  volume    = {79},
  journal   = {Stahlbau},
  number    = {5},
  publisher = {Ernst \& Sohn},
  address   = {Berlin},
  issn      = {1437-1049 (E-Journal); 0038-9145 (Print)},
  doi       = {10.1002/stab.201001330},
  pages     = {345 -- 355},
  year      = {2010},
  abstract  = {Die Anforderungen an das energiesparende Bauen sind mit der Einf{\"u}hrung der Energieeinsparverordnung (EnEV) 2009 auch im Industrie- und Gewerbebau deutlich versch{\"a}rft worden. Einen wesentlichen Beitrag zur Energieeinsparung liefert die Minimierung des Transmissionsw{\"a}rmetransfers. Analysiert man Geb{\"a}udeh{\"u}llen in Metallleichtbauweise stellt man fest, dass eine Erh{\"o}hung der W{\"a}rmed{\"a}mmst{\"a}rke allein noch nicht zielf{\"u}hrend ist, zus{\"a}tzlich sind W{\"a}rmebr{\"u}ckeneffekte zu ber{\"u}cksichtigen und deren Einfl{\"u}sse auf die W{\"a}rmetransmission zu reduzieren. Neben der Bedeutung f{\"u}r die Energieeinsparung ist eine w{\"a}rmetechnisch optimierte Detailausbildung auch erforderlich, um einen ausreichenden Feuchteschutz (Vermeidung von Tauwasser und Schimmelpilz) zu realisieren und so Sch{\"a}den zu vermeiden. Ein wichtiges Hilfsmittel stellt hierzu der vom Industrieverband f{\"u}r Bausysteme im Metallleichtbau (IFBS) herausgegebene W{\"a}rmebr{\"u}ckenatlas der Metall-Sandwichbauweise dar.},
  language  = {de}
}
@article{MukherjeePrahlBlecketal.2010,
  author    = {Mukherjee, Krishnendu and Prahl, Ulrich and Bleck, Wolfgang and Reisgen, Uwe and Schleser, Markus and Abdurakhmanov, Aydemir},
  title     = {Characterization and modelling techniques for gas metal arc welding of DP 600 sheet steels},
  series = {Materialwissenschaft und Werkstofftechnik},
  volume    = {41},
  journal   = {Materialwissenschaft und Werkstofftechnik},
  number    = {11},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1521-4052},
  doi       = {10.1002/mawe.201000692},
  pages     = {972 -- 983},
  year      = {2010},
  abstract  = {The objectives of the present work are to characterize the Gas Metal Arc Welding process of DP 600 sheet steel and to summarize the modelling techniques. The time-temperature evolution during the welding cycle was measured experimentally and modelled with the softwaretool SimWeld. To model the phase transformations during the welding cycle dilatometer tests were done to quantify the parameters for phase field modelling by MICRESS®. The important input parameters are interface mobility, nucleation density, etc. A contribution was made to include austenite to bainite transformation in MICRESS®. This is useful to predict the microstructure in the fast cooling segments. The phase transformation model is capable to predict the microstructure along the heating and cooling cycles of welding. Tensile tests have shown the evidence of failure at the heat affected zone, which has the ferrite-tempered martensite microstructure.},
  language  = {en}
}
@article{ReisgenSchleserMokrovetal.2010,
  author    = {Reisgen, Uwe and Schleser, Markus and Mokrov, Oleg and Ahmed, Essam},
  title     = {Shielding gas influences on laser weldability of tailored blanks of advanced automotive steels},
  series = {Applied surface science},
  volume    = {257},
  journal   = {Applied surface science},
  number    = {5},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0169-4332 (E-Journal); 0169-4332 (Print)},
  doi       = {10.1016/j.apsusc.2010.08.042},
  pages     = {1401 -- 1406},
  year      = {2010},
  abstract  = {The effects of shielding gas types and flow rates on CO2 laser weldability of DP600/TRIP700 steel sheets were studied in this work. The evaluated shielding gases were helium (He), argon (Ar) and different mixtures of He and Ar. Weld penetration, tensile strength and formability (Erichsen test) of laser welds were found to be strongly dependent upon the shielding gas types. The ability of shielding gas in removing plasma plume and thus increasing weld penetration is believed to be closely related to ionization potential and atomic weight which determine the period of plasma formation and disappearance. It was found that the higher helium shielding gas flow rate, the deeper weld penetration and the lower weld width.},
  language  = {en}
}
@article{AhmedReisgenSchleseretal.2010,
  author    = {Ahmed, Essam and Reisgen, Uwe and Schleser, Markus and Mokrov, Oleg},
  title     = {On formability of tailor laser welded blanks of DP/TRIP steel sheets},
  series = {Science and technology of welding and joining},
  volume    = {15},
  journal   = {Science and technology of welding and joining},
  number    = {5},
  publisher = {Maney},
  address   = {Leeds},
  issn      = {1743-2936},
  doi       = {10.1179/136217110X12731414739754},
  pages     = {337 -- 342},
  year      = {2010},
  abstract  = {This paper aims to evaluate the formability of tailor welded blanks of dual phase (DP600)/transformation induced plasticity (TRIP700) steel sheets. In this work, bead on plate butt joints of 2·5 mm DP600 and 1·2 mm TRIP700 steel sheets were performed using CO2 laser beam welding. Microhardness measurements and transverse tensile testing were carried out to characterise the welds. The formability of base metals and welds were investigated by standard Erichsen test. In a perpendicular tensile test to the weld line, all specimens were fractured at the TRIP base metal, and the strengths were somewhat higher than those of base metal. There was a significant reduction in formability caused by welding of the DP600/TRIP700 steel sheets, and the formability increased with increasing welding speed.},
  language  = {en}
}
@article{KuhnhenneDoeringKockeretal.2010,
  author    = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Kocker, Ronald and Pyschny, Dominik and Feldmann, Markus},
  title     = {Die {\"O}kobilanz als Baustein der Nachhaltigkeitsbewertung im Industrie- und Gewerbebau},
  series = {Stahlbau},
  volume    = {79},
  journal   = {Stahlbau},
  number    = {6},
  publisher = {Ernst \& Sohn},
  address   = {Berlin},
  issn      = {1437-1049 (E-Journal); 0038-9145 (Print)},
  doi       = {10.1002/stab.201001336},
  pages     = {439 -- 447},
  year      = {2010},
  abstract  = {Im Herbst 2009 wurde das "Deutsche G{\"u}tesiegel Nachhaltiges Bauen" f{\"u}r die Geb{\"a}udetypen Industriebauten und Handelsbauten offiziell vorgestellt. Die {\"O}kobilanz als wichtiger Baustein der Nachhaltigkeitsbewertung wird somit auch im Industrie- und Gewerbebau zuk{\"u}nftig stark an Bedeutung gewinnen. Der vorliegende Beitrag beinhaltet einen Vergleich der Umweltleistung verschiedener Bauweisen des Industrie- und Gewerbebaus, die durch die {\"O}kobilanzierung quantifizierbar wird, anhand von momentan verf{\"u}gbaren Daten und Methoden. Es werden ausgew{\"a}hlte Ergebnisse sowie vorhandene Unsicherheiten und daraus resultierender Forschungsbedarf aufgezeigt.},
  language  = {de}
}
@article{Goldbach2010,
  author    = {Goldbach, Daniel},
  title     = {Fahrstabilit{\"a}tserh{\"o}hung bei Gespannen durch Querverschiebung des Anh{\"a}ngerkupplungspunktes},
  series = {mechatronik mobil},
  volume    = {2},
  journal   = {mechatronik mobil},
  number    = {1},
  publisher = {Expert Verlag},
  address   = {Renningen},
  issn      = {1867-7371},
  pages     = {23 -- 29},
  year      = {2010},
  language  = {de}
}
@article{HeinzelSchaeferMuelleretal.2010,
  author    = {Heinzel, Alexander and Sch{\"a}fer, Ralf and M{\"u}ller, Hans-Wilhelm and Schieffer, Andre and Ingenhag, Ariane and Eickhoff, Simon B. and Northoff, Georg and Franz, Matthias and Hautzel, Hubertus},
  title     = {Increased Activation of the Supragenual Anterior Cingulate Cortex during Visual Emotional Processing in Male Subjects with High Degrees of Alexithymia: An Event-Related fMRI Study},
  series = {Psychotherapy and Psychosomatics},
  volume    = {79},
  journal   = {Psychotherapy and Psychosomatics},
  number    = {6},
  publisher = {Karger},
  address   = {Basel},
  issn      = {0033-3190},
  doi       = {10.1159/000320121},
  pages     = {363 -- 370},
  year      = {2010},
  abstract  = {Background: One of the most prominent neurobiological models of alexithymia assumes an altered function of the anterior cingulate cortex (ACC) as the crucial neural correlate of alexithymia. So far functional imaging studies have yielded inconclusive results. Therefore, we tested this hypothesis in healthy alexithymics and nonalexithymics in an event-related fMRI study. Methods: Thirty high- and 30 low-alexithymic right-handed male subjects (selected by the 20-item Toronto Alexithymia Scale, TAS-20) were investigated with event-related fMRI using a picture viewing paradigm. The stimuli consisted of happy, fearful and neutral facial expressions (Ekman-Friesen) as well as positive, negative and neutral pictures from the International Affective Picture System. Results: Contrasting the high-alexithymic with the low-alexithymic group we observed increased activation of the supragenual ACC for different emotional valences as well as for different emotional stimuli. Moreover, there was a positive correlation of the ACC with the individual TAS-20 scores but no correlations with the individual Beck Depression Inventory scores. Additionally, there was no difference in activity of the amygdala. Conclusions: We demonstrated that the supragenual ACC is constantly activated more strongly in alexithymic subjects and that this activation is related to the symptoms of alexithymia and not to associated symptoms such as depression. Therefore, our findings support the hypothesis of an altered function of the ACC in alexithymia.},
  language  = {en}
}