@article{EijckDemmelArtmannetal.2011, author = {Eijck, Lambert van and Demmel, Franz and Artmann, Gerhard and Stadtler, Andreas Maximilian}, title = {Macromolecular dynamics in red blood cells investigated using neutron spectroscopy}, series = {Journal of the Royal Society Interface}, volume = {8}, journal = {Journal of the Royal Society Interface}, number = {57}, publisher = {The Royal Society}, address = {London}, isbn = {1742-5689}, pages = {590 -- 600}, year = {2011}, language = {en} } @article{KurulganDemirciDemirciTrzewiketal.2011, author = {Kurulgan Demirci, Eylem and Demirci, T. and Trzewik, J{\"u}rgen and Linder, Peter and Karakulah, G. and Artmann, Gerhard and Sakizli, M. and Temiz Artmann, Ayseg{\"u}l}, title = {Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation}, series = {Cellular and molecular bioengineering. 4 (2011), H. 1}, journal = {Cellular and molecular bioengineering. 4 (2011), H. 1}, publisher = {Springer}, address = {Berlin}, isbn = {1865-5025}, pages = {46 -- 55}, year = {2011}, language = {en} } @article{AminTemizArtmannArtmannetal.2009, author = {Amin, Rashid and Temiz Artmann, Ayseg{\"u}l and Artmann, Gerhard and Lazarovici, Philip and Lelkes, Peter I.}, title = {Permeability of an In Vitro Model of Blood Brain Barrier (BBB)}, series = {13th International Conference on Biomedical Engineering / Lim, Chwee Teck [Ed.]}, journal = {13th International Conference on Biomedical Engineering / Lim, Chwee Teck [Ed.]}, isbn = {978-3-540-92841-6}, pages = {81 -- 84}, year = {2009}, language = {en} } @article{LeimenaArtmannDachwaldetal.2010, author = {Leimena, W. and Artmann, Gerhard and Dachwald, Bernd and Temiz Artmann, Ayseg{\"u}l and Gossmann, Matthias and Digel, Ilya}, title = {Feasibility of an in-situ microbial decontamination of an ice-melting probe}, series = {Eurasian Chemico-Technological Journal. 12 (2010), H. 2}, journal = {Eurasian Chemico-Technological Journal. 12 (2010), H. 2}, isbn = {1562-3920}, pages = {145 -- 150}, year = {2010}, language = {en} } @article{KurulganDemirciDemirciLinderetal.2012, author = {Kurulgan Demirci, Eylem and Demirci, Taylan and Linder, Peter and Trzewik, J{\"u}rgen and Gierkowski, Jessica Ricarda and Gossmann, Matthias and Kayser, Peter and Porst, Dariusz and Digel, Ilya and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells}, series = {Journal of Bioscience and Bioengineering}, volume = {113}, journal = {Journal of Bioscience and Bioengineering}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1347-4421}, doi = {10.1016/j.jbiosc.2012.03.019}, pages = {212 -- 219}, year = {2012}, abstract = {All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.}, language = {en} } @book{Artmann2011, author = {Artmann, Gerhard}, title = {Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds.}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-11864-7}, pages = {XLI, 541 S. : Ill., graph. Darst.}, year = {2011}, language = {en} } @article{BassamHeschelerTemizArtmannetal.2012, author = {Bassam, Rasha and Hescheler, J{\"u}rgen and Temiz Artmann, Ayseg{\"u}l and Artmann, Gerhard and Digel, Ilya}, title = {Effects of spermine NONOate and ATP on the thermal stability of hemoglobin}, series = {BMC Biophysics}, volume = {5}, journal = {BMC Biophysics}, publisher = {BioMed Central}, address = {London}, issn = {2046-1682}, doi = {10.1186/2046-1682-5-16}, pages = {Art. 16}, year = {2012}, abstract = {Background Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min. Results Major results were: 1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment, 2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and 3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature. Conclusion The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell.}, language = {en} } @article{StadlerDigelEmbsetal.2009, author = {Stadler, Andreas M. and Digel, Ilya and Embs, Jan P. and Unruh, Tobias and Tehei, M. and Zaccai, G. and B{\"u}ldt, G. and Artmann, Gerhard}, title = {From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature}, series = {Biophysical Journal. 96 (2009), H. 12}, journal = {Biophysical Journal. 96 (2009), H. 12}, publisher = {Cell Press}, address = {Cambridge, Mass.}, isbn = {0006-3495}, pages = {5073 -- 5081}, year = {2009}, language = {en} } @article{StadlerEmbsDigeletal.2008, author = {Stadler, Andreas M. and Embs, Jan P. and Digel, Ilya and Artmann, Gerhard and Unruh, Tobias and B{\"u}ldt, Georg and Zaccai, Guiseppe}, title = {Cytoplasmic water and hydration layer dynamics in human red blood cells}, series = {Journal of the American Chemical Society. 50 (2008), H. 130}, journal = {Journal of the American Chemical Society. 50 (2008), H. 130}, isbn = {1520-5126}, pages = {16852 -- 16853}, year = {2008}, language = {en} } @article{DigelKayserArtmann2008, author = {Digel, Ilya and Kayser, Peter and Artmann, Gerhard}, title = {Molecular processes in biological thermosensation}, series = {Journal of Biophysics. 2008 (2008)}, journal = {Journal of Biophysics. 2008 (2008)}, isbn = {1687-8000}, pages = {1 -- 9}, year = {2008}, language = {en} }