@article{MolinnusSorichBartzetal.2016, author = {Molinnus, Denise and Sorich, Maren and Bartz, Alexander and Siegert, Petra and Willenberg, Holger S. and Lisdat, Fred and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Towards an adrenaline biosensor based on substrate recycling amplification in combination with an enzyme logic gate}, series = {Sensors and Actuators B: Chemical}, volume = {237}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.06.064}, pages = {190 -- 195}, year = {2016}, abstract = {An amperometric biosensor using a substrate recycling principle was realized for the detection of low adrenaline concentrations (1 nM) by measurements in phosphate buffer and Ringer's solution at pH 6.5 and pH 7.4, respectively. In proof-of-concept experiments, a Boolean logic-gate principle has been applied to develop a digital adrenaline biosensor based on an enzyme AND logic gate. The obtained results demonstrate that the developed digital biosensor is capable for a rapid qualitative determination of the presence/absence of adrenaline in a YES/NO statement. Such digital biosensor could be used in clinical diagnostics for the control of a correct insertion of a catheter in the adrenal veins during adrenal venous-sampling procedure.}, language = {en} } @article{TakenagaBiselliSchnitzleretal.2014, author = {Takenaga, Shoko and Biselli, Manfred and Schnitzler, Thomas and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing}, series = {Physica status solidi A : Applications and materials science}, volume = {211}, journal = {Physica status solidi A : Applications and materials science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330464}, pages = {1410 -- 1415}, year = {2014}, abstract = {The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.}, language = {en} } @article{RoehlenPilasDahmenetal.2018, author = {R{\"o}hlen, Desiree and Pilas, Johanna and Dahmen, Markus and Keusgen, Michael and Selmer, Thorsten and Sch{\"o}ning, Michael Josef}, title = {Toward a Hybrid Biosensor System for Analysis of Organic and Volatile Fatty Acids in Fermentation Processes}, series = {Frontiers in Chemistry}, journal = {Frontiers in Chemistry}, number = {6}, publisher = {Frontiers}, address = {Lausanne}, doi = {10.3389/fchem.2018.00284}, pages = {Artikel 284}, year = {2018}, abstract = {Monitoring of organic acids (OA) and volatile fatty acids (VFA) is crucial for the control of anaerobic digestion. In case of unstable process conditions, an accumulation of these intermediates occurs. In the present work, two different enzyme-based biosensor arrays are combined and presented for facile electrochemical determination of several process-relevant analytes. Each biosensor utilizes a platinum sensor chip (14 × 14 mm²) with five individual working electrodes. The OA biosensor enables simultaneous measurement of ethanol, formate, d- and l-lactate, based on a bi-enzymatic detection principle. The second VFA biosensor provides an amperometric platform for quantification of acetate and propionate, mediated by oxidation of hydrogen peroxide. The cross-sensitivity of both biosensors toward potential interferents, typically present in fermentation samples, was investigated. The potential for practical application in complex media was successfully demonstrated in spiked sludge samples collected from three different biogas plants. Thereby, the results obtained by both of the biosensors were in good agreement to the applied reference measurements by photometry and gas chromatography, respectively. The proposed hybrid biosensor system was also used for long-term monitoring of a lab-scale biogas reactor (0.01 m³) for a period of 2 months. In combination with typically monitored parameters, such as gas quality, pH and FOS/TAC (volatile organic acids/total anorganic carbonate), the amperometric measurements of OA and VFA concentration could enhance the understanding of ongoing fermentation processes.}, language = {en} } @article{ImmelGrothHuhnetal.2011, author = {Immel, Timo A. and Groth, Ulrich and Huhn, Thomas and {\"O}hlschl{\"a}ger, Peter}, title = {Titanium salan complexes displays strong antitumor properties in vitro and in vivo in mice}, series = {PLoS ONE}, volume = {6}, journal = {PLoS ONE}, number = {3}, publisher = {Plos}, address = {San Francisco, California, US}, doi = {10.1371/journal.pone.0017869}, pages = {e17869}, year = {2011}, abstract = {The anticancer activity of titanium complexes has been known since the groundbreaking studies of K{\"o}pf and K{\"o}pf-Maier on titanocen dichloride. Unfortunately, possibly due to their fast hydrolysis, derivatives of titanocen dichloride failed in clinical studies. Recently, the new family of titanium salan complexes containing tetradentate ONNO ligands with anti-cancer properties has been discovered. These salan complexes are much more stabile in aqueous media. In this study we describe the biological activity of two titanium salan complexes in a mouse model of cervical cancer. High efficiency of this promising complex family was demonstrated for the first time in vivo. From these data we conclude that titanium salan complexes display very strong antitumor properties exhibiting only minor side effects. Our results may influence the chemotherapy with metallo therapeutics in the future.}, language = {en} } @misc{Lauth1997, author = {Lauth, Jakob}, title = {Titan-Verbindungen mit Hydrotalcit-Struktur : Offenlegungsschrift DE19523053A1 ; Ver{\"o}ffentlichungstag im Patentblatt : 02.01.1997 / Anmelder: BASF AG, 67063 Ludwigshafen}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {6 S. . graph. Darst.}, year = {1997}, language = {de} } @misc{Lauth1997, author = {Lauth, Jakob}, title = {Titan-Verbindungen mit Hydrotalcit-Struktur : Europ{\"a}ische Offenlegungsschrift EP0749941A1; Ver{\"o}ffentlichungstag im Patentblatt : 06.02.1997 / Anmelder: BASF AG, 67063 Ludwigshafen}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {6 S. . graph. Darst.}, year = {1997}, language = {de} } @article{Mang2004, author = {Mang, Thomas}, title = {Thermosensitive Magnetic Nanoparticles for Bioanalytical and Therapeutical Applications}, series = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, journal = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, isbn = {0013-5585}, pages = {1006 -- 1007}, year = {2004}, language = {en} } @article{FeuerriegelStahlbergRiegeletal.1995, author = {Feuerriegel, Uwe and Stahlberg, R. and Riegel, J. and Steiger, F.}, title = {Thermoselect - Energie- und Rohstoffgewinnung: Teil 3. Massen- und Energiebilanz unter Ber{\"u}cksichtigung der Wirtschaftlichkeit der Synthesegasnutzung. Stahlberg, R.; Riegel, J.; Feuerriegel, U.; Steiger, F.}, series = {Chemische Technik . 47 (1995), H. 3}, journal = {Chemische Technik . 47 (1995), H. 3}, isbn = {0045-6519}, pages = {113 -- 121}, year = {1995}, language = {de} } @article{FeuerriegelStahlberg1995, author = {Feuerriegel, Uwe and Stahlberg, R.}, title = {Thermoselect - Energie- und Rohstoffgewinnung: Teil 2. Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualit{\"a}ten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffstr{\"o}men. Stahlberg, R. ; Feuerriegel, U.}, series = {Chemische Technik . 47 (1995), H. 1}, journal = {Chemische Technik . 47 (1995), H. 1}, isbn = {0045-6519}, pages = {1 -- 10}, year = {1995}, language = {de} } @article{FeuerriegelStahlberg1994, author = {Feuerriegel, Uwe and Stahlberg, R.}, title = {Thermoselect - Energie- und Rohstoffgewinnung: Teil 1. Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabf{\"a}llen. Stahlberg, R. ; Feuerriegel, U.}, series = {Chemische Technik . 46 (1994), H. 5}, journal = {Chemische Technik . 46 (1994), H. 5}, isbn = {0045-6519}, pages = {257 -- 266}, year = {1994}, language = {en} }