@article{KuhnhenneDoeringFeldmann2010, author = {Kuhnhenne, Markus and D{\"o}ring, Bernd and Feldmann, Markus}, title = {Grunds{\"a}tze und L{\"o}sungen zur W{\"a}rmebr{\"u}ckenreduktion im Metallleichtbau}, series = {Stahlbau}, volume = {79}, journal = {Stahlbau}, number = {5}, publisher = {Ernst \& Sohn}, address = {Berlin}, issn = {1437-1049 (E-Journal); 0038-9145 (Print)}, doi = {10.1002/stab.201001330}, pages = {345 -- 355}, year = {2010}, abstract = {Die Anforderungen an das energiesparende Bauen sind mit der Einf{\"u}hrung der Energieeinsparverordnung (EnEV) 2009 auch im Industrie- und Gewerbebau deutlich versch{\"a}rft worden. Einen wesentlichen Beitrag zur Energieeinsparung liefert die Minimierung des Transmissionsw{\"a}rmetransfers. Analysiert man Geb{\"a}udeh{\"u}llen in Metallleichtbauweise stellt man fest, dass eine Erh{\"o}hung der W{\"a}rmed{\"a}mmst{\"a}rke allein noch nicht zielf{\"u}hrend ist, zus{\"a}tzlich sind W{\"a}rmebr{\"u}ckeneffekte zu ber{\"u}cksichtigen und deren Einfl{\"u}sse auf die W{\"a}rmetransmission zu reduzieren. Neben der Bedeutung f{\"u}r die Energieeinsparung ist eine w{\"a}rmetechnisch optimierte Detailausbildung auch erforderlich, um einen ausreichenden Feuchteschutz (Vermeidung von Tauwasser und Schimmelpilz) zu realisieren und so Sch{\"a}den zu vermeiden. Ein wichtiges Hilfsmittel stellt hierzu der vom Industrieverband f{\"u}r Bausysteme im Metallleichtbau (IFBS) herausgegebene W{\"a}rmebr{\"u}ckenatlas der Metall-Sandwichbauweise dar.}, language = {de} } @book{BuyerKleinMueller2010, author = {Buyer, Christoph and Klein, Hartmut and M{\"u}ller, Thomas}, title = {{\"A}nderung der Unternehmensform : Handbuch zum Umwandlungs- und Umwandlungssteuerrecht}, edition = {8. Aufl.}, publisher = {Verl. Neue Wirtschafts-Briefe}, address = {Herne}, isbn = {978-3-482-42258-4}, pages = {XL, 585 S.}, year = {2010}, language = {de} } @article{Goldbach2010, author = {Goldbach, Daniel}, title = {Fahrstabilit{\"a}tserh{\"o}hung bei Gespannen durch Querverschiebung des Anh{\"a}ngerkupplungspunktes}, series = {mechatronik mobil}, volume = {2}, journal = {mechatronik mobil}, number = {1}, publisher = {Expert Verlag}, address = {Renningen}, issn = {1867-7371}, pages = {23 -- 29}, year = {2010}, language = {de} } @inproceedings{Goldbach2010, author = {Goldbach, Daniel}, title = {Management der funktionalen Sicherheit einer Entwicklung eines sicherheitskritischen Systems im Bereich Nutzfahrzeuge}, series = {Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)}, booktitle = {Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)}, publisher = {Shaker}, address = {Aachen}, isbn = {978-3-8322-9040-5}, pages = {487 -- 495}, year = {2010}, language = {de} } @inproceedings{GoldbachAment2010, author = {Goldbach, Daniel and Ament, Christoph}, title = {Stabilized reversing with vehicle trailer combinations}, series = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau}, booktitle = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau}, editor = {Scharff, Peter}, publisher = {Verl. ISLE}, address = {Ilmenau}, organization = {Technische Universit{\"a}t / Fakult{\"a}t f{\"u}r Informatik und Automatisierung}, isbn = {978-3-938843-53-6}, pages = {352 -- 357}, year = {2010}, language = {en} } @misc{Alt2010, author = {Alt, Helmut}, title = {Vom Studium der Kerntechnik zu Energie zwischen Wunsch und Wirklichkeit : [Vortrag : Verleihung der KTG-Ehrenmitgliedschaft, Berlin, November 2010]}, pages = {1 -- 15}, year = {2010}, language = {de} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @misc{SiegertMussmannO'Connelletal.2010, author = {Siegert, Petra and Mussmann, Nina and O'Connell, Timothy and Maurer, Karl-Heinz}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt / WIPO}, address = {M{\"u}nchen / Genf}, pages = {1 -- 30}, year = {2010}, language = {de} } @misc{SiegertBaumstarkKluinetal.2010, author = {Siegert, Petra and Baumstark, Rebecca and Kluin, Cornelia and O'Connell, Timothy and Maurer, Karl-Heinz and Hellmuth, Hendrik}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {1 -- 30}, year = {2010}, language = {de} } @misc{SiegertSpitzMaurer2010, author = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patentamt / WIPO}, address = {M{\"u}nchen / Genf}, pages = {1 -- 31}, year = {2010}, language = {de} }