@article{BalakrishnanAndreiSelmerSelmeretal.2010,
  author    = {Balakrishnan, Karthikeyan and Andrei-Selmer, Luminita-Cornelia and Selmer, Thorsten and Bacher, Michael and Dodel, Richard},
  title     = {Comparison of intravenous immunoglobulins for naturally occurring autoantibodies against amyloid-β},
  series = {Journal of Alzheimer's Disease},
  volume    = {20},
  journal   = {Journal of Alzheimer's Disease},
  number    = {1},
  publisher = {IOS Press},
  address   = {Amsterdam},
  issn      = {1387-2877},
  doi       = {10.3233/JAD-2010-1353},
  pages     = {135 -- 143},
  year      = {2010},
  abstract  = {Intravenous immunoglobulins (IVIG) are currently used for therapeutic purposes in autoimmune disorders. Recently, we demonstrated the presence of naturally occurring antibodies against amyloid- β (nAbs-Aβ) within the pool of IVIG. In this study, we compared different brands of IVIG for nAbs-Aβ and have found differences in the specificity of the nAbs-Aβ towards Aβ1-40 and Aβ1-42 . We analyzed the influence of a pH-shift over the course of antibody storage using ELISA and investigated antibody dimerization at acidic and neutral pH as well as differences in the IgG subclass distributions among the IVIG using both HPLC and a nephelometric assay. Furthermore, we investigated the epitope region of purified nAbs-Aβ. The differences found in Aβ specificity are not directly proportionate to the binding nature of these antibodies when administered in vivo. This information, however, may serve as a guide when choosing the commercial source of IVIG for therapeutic applications in Alzheimer's disease},
  language  = {en}
}
@article{TixMollKrafftetal.2024,
  author    = {Tix, Julian and Moll, Fabian and Krafft, Simone and Betsch, Matthias and Tippk{\"o}tter, Nils},
  title     = {Hydrogen production from enzymatic pretreated organic waste with thermotoga neapolitana},
  series = {Energies},
  volume    = {17},
  journal   = {Energies},
  number    = {12},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {1996-1073},
  doi       = {10.3390/en17122938},
  pages     = {20 Seiten},
  year      = {2024},
  abstract  = {Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H2) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481\% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L-1. In the subsequent set fermentation in serum bottles, a H2 yield of 1.26 mmol H2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83\% could be measured in the overhead space.},
  language  = {en}
}
@article{VarrialeHengsbachGuoetal.2024,
  author    = {Varriale, Ludovica and Hengsbach, Jan-Niklas and Guo, Tianyi and Kuka, Katrin and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Sustainable production of lactic acid using a perennial ryegrass as feedstock—a comparative study of fermentation at the bench- and reactor-scale, and ensiling},
  series = {Sustainability},
  volume    = {16},
  journal   = {Sustainability},
  number    = {18},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2071-1050},
  doi       = {10.3390/su16188054},
  year      = {2024},
  abstract  = {Perennial ryegrass (Lolium perenne) is an underutilized lignocellulosic biomass that has several benefits such as high availability, renewability, and biomass yield. The grass press-juice obtained from the mechanical pretreatment can be used for the bio-based production of chemicals. Lactic acid is a platform chemical that has attracted consideration due to its broad area of applications. For this reason, the more sustainable production of lactic acid is expected to increase. In this work, lactic acid was produced using complex medium at the bench- and reactor scale, and the results were compared to those obtained using an optimized press-juice medium. Bench-scale fermentations were carried out in a pH-control system and lactic acid production reached approximately 21.84 ± 0.95 g/L in complex medium, and 26.61 ± 1.2 g/L in press-juice medium. In the bioreactor, the production yield was 0.91 ± 0.07 g/g, corresponding to a 1.4-fold increase with respect to the complex medium with fructose. As a comparison to the traditional ensiling process, the ensiling of whole grass fractions of different varieties harvested in summer and autumn was performed. Ensiling showed variations in lactic acid yields, with a yield up to 15.2\% dry mass for the late-harvested samples, surpassing typical silage yields of 6-10\% dry mass.},
  language  = {en}
}
@article{UndenBeckerBongaertsetal.1995,
  author    = {Unden, Gottfried and Becker, S. and Bongaerts, Johannes and Holighaus, G. and Schirawski, Jan and Six, Simon},
  title     = {O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria},
  series = {Archives of microbiology},
  volume    = {Vol. 164},
  journal   = {Archives of microbiology},
  number    = {Iss. 2},
  issn      = {1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)},
  pages     = {81 -- 90},
  year      = {1995},
  language  = {en}
}
@article{UndenBeckerBongaertsetal.1994,
  author    = {Unden, Gottfried and Becker, S. and Bongaerts, Johannes and Schirawski, Jan and Six, Simon},
  title     = {Oxygen regulated gene expression in facultatively anaerobic bacteria},
  series = {Antonie van Leeuwenhoek},
  volume    = {Vol. 66},
  journal   = {Antonie van Leeuwenhoek},
  number    = {Iss. 1-3},
  issn      = {0003-6072 (Print) ; 1572-9699 (online)},
  pages     = {3 -- 22},
  year      = {1994},
  language  = {en}
}
@article{ElbersPfefferFrieseletal.1993,
  author    = {Elbers, Gereon and Pfeffer, H.-U. and Friesel, J. and Beier, Reinhold},
  title     = {Pilotuntersuchungen gem{\"a}ß dem Verordnungsentwurf nach \S 40, Abs. 2 des Bundesimmissionsschutzgesetzes / H.-U. Pfeffer, J. Friesel, G. Elbers, R. Beier, K. Ellermann},
  series = {Drei Jahrzehnte Luftqualit{\"a}ts{\"u}berwachung : Vortr{\"a}ge und Berichte zum Kolloquium "Drei Jahrzehnte Luftqualit{\"a}ts{\"u}berwachung - Bilanz und Perspektiven" am 11. November 1993 in Essen / [Hrsg.: Landesanstalt f{\"u}r Immissionsschutz Nordrhein-Westfalen. Red. Bearb.: Robert Niesenhaus]},
  journal   = {Drei Jahrzehnte Luftqualit{\"a}ts{\"u}berwachung : Vortr{\"a}ge und Berichte zum Kolloquium "Drei Jahrzehnte Luftqualit{\"a}ts{\"u}berwachung - Bilanz und Perspektiven" am 11. November 1993 in Essen / [Hrsg.: Landesanstalt f{\"u}r Immissionsschutz Nordrhein-Westfalen. Red. Bearb.: Robert Niesenhaus]},
  address   = {Essen},
  pages     = {117},
  year      = {1993},
  language  = {de}
}
@article{RibitschKarlBirnerGruenbergeretal.2010,
  author    = {Ribitsch, Doris and Karl, Wolfgang and Birner-Gruenberger, Ruth and Gruber, Karl and Eiteljoerg, Inge and Remler, Peter and Wieland, Susanne and Siegert, Petra and Maurer, Karl-Heinz and Schwab, Helmut},
  title     = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli},
  series = {Journal of biotechnology},
  volume    = {150},
  journal   = {Journal of biotechnology},
  number    = {3},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2010.09.947},
  pages     = {408 -- 416},
  year      = {2010},
  abstract  = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.},
  language  = {en}
}
@article{AdelsThoennessenMonakhova2024,
  author    = {Adels, Klaudia and Th{\"o}nnessen, Vera and Monakhova, Yulia},
  title     = {Complementary instrumental techniques applied to pain relieving tablets in an undergraduate laboratory experiment},
  series = {Journal of Chemical Education},
  journal   = {Journal of Chemical Education},
  publisher = {ACS},
  issn      = {0021-9584 (Print)},
  doi       = {10.1021/acs.jchemed.4c00681},
  pages     = {11 Seiten},
  year      = {2024},
  abstract  = {Several unconnected laboratory experiments are usually offered for students in instrumental analysis lab. To give the students a more rational overview of the most common instrumental techniques, a new laboratory experiment was developed. Marketed pain relief drugs, familiar consumer products with one to three active components, namely, acetaminophen (paracetamol), acetylsalicylic acid (ASA), and caffeine, were selected. Common analytical methods were compared regarding the performance of qualitative and quantitative analysis of unknown tablets: UV-visible (UV-vis), infrared (IR), and nuclear magnetic resonance (NMR) spectroscopies, as well as high-performance liquid chromatography (HPLC). The students successfully uncovered the composition of formulations, which were divided into three difficulty categories. Students were shown that in addition to simple mixtures handled in theoretical classes, the composition of complex drug products can also be uncovered. By comparing the performance of different techniques, students deepen their understanding and compare the efficiency of analytical methods in the context of complex mixtures. The laboratory experiment can be adjusted for graduate level by including extra tasks such as method optimization, validation, and 2D spectroscopic techniques.},
  language  = {en}
}
@article{RaueWambachGloeggleretal.2014,
  author    = {Raue, Markus and Wambach, M. and Gl{\"o}ggler, Stefan and Grefen, Dana and Kaufmann, R. and Abetz, C. and Georgopanos, Prokopios and Handge, U. A. and Mang, Thomas and Bl{\"u}mich, Bernhard and Abetz, V.},
  title     = {Investigation of historical hard rubber ornaments of Charles Goodyear},
  series = {Macromolecular chemistry and physics},
  volume    = {Vol. 215},
  journal   = {Macromolecular chemistry and physics},
  number    = {No. 3},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1022-1352},
  pages     = {245 -- 254},
  year      = {2014},
  language  = {en}
}
@article{BiselliSchmidtBuechsetal.1999,
  author    = {Biselli, Manfred and Schmidt, Sebastian and B{\"u}chs, Jochen and Born, Christoph},
  title     = {A new correlation for the wall-to-fluid mass transfer in liquid-sol fluidized beds / Schmidt, S. ; B{\"u}chs, J. ; Born, C. ; Biselli, M.},
  series = {Chemical Engineering Science. 54 (1999), H. 6},
  journal   = {Chemical Engineering Science. 54 (1999), H. 6},
  isbn      = {0009-2509},
  pages     = {829 -- 839},
  year      = {1999},
  language  = {en}
}