@inproceedings{HeinzOertelBung2010, author = {Heinz, G. and Oertel, Mario and Bung, Daniel B.}, title = {Investigations on a cooling water system for a gas tubine facility concerning air vortexes and sediment transport}, series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, editor = {Janssen, Robert}, publisher = {School of Civil Engineering, The University of Queensland}, address = {Brisbane}, organization = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>}, isbn = {9781742720159}, pages = {109 -- 116}, year = {2010}, language = {en} } @inproceedings{KerpenBungSchlurmann2010, author = {Kerpen, N. B. and Bung, Daniel B. and Schlurmann, Torsten}, title = {Physical model investigations of ships passing through a lock}, series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, editor = {Janssen, Robert}, publisher = {School of Civil Engineeering, The University of Queensland}, address = {Brisbane}, organization = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>}, isbn = {9781742720159}, pages = {93 -- 100}, year = {2010}, language = {en} } @inproceedings{BungSchlenkhoff2010, author = {Bung, Daniel B. and Schlenkhoff, Andreas}, title = {Self-aerated skimming flow on embankment stepped spillways : the effect of additional micro-roughness on energy dissipation and oxygen transfer}, series = {Proceedings from first IAHR European congress : May 2010, Edinburgh}, booktitle = {Proceedings from first IAHR European congress : May 2010, Edinburgh}, publisher = {Heriot-Watt University, School of the Built Environment}, address = {Edinburgh}, organization = {International Association for Hydraulic Engineering and Research}, isbn = {9780956595102}, pages = {Artikelkennnummer HSIIId}, year = {2010}, language = {en} } @inproceedings{KerpenBungSchlurmann2010, author = {Kerpen, N. B. and Bung, Daniel B. and Schlurmann, Torsten}, title = {Physical model investigations of pressure distributions next to ships passing through a lock}, series = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, booktitle = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, publisher = {Univ. Press}, address = {Tianjin}, organization = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <5, 2010, Tianjin>}, isbn = {978-7-5618-3671-2}, pages = {514 -- 519}, year = {2010}, language = {en} } @inproceedings{Bung2010, author = {Bung, Daniel B.}, title = {A comparative study of self-aerated stepped spillway and smooth invert chute flow: the effect of step-induced macro roughness}, series = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, booktitle = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, publisher = {Univ. Press}, address = {Tianjin}, organization = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <5, 2010, Tianjin>}, isbn = {978-7-5618-3671-2}, pages = {451 -- 456}, year = {2010}, language = {en} } @inproceedings{FeldhusenBrezingPuetzetal.2010, author = {Feldhusen, J{\"o}rg and Brezing, Alexander Nikolaus and P{\"u}tz, Claus and W{\"a}hlisch, Georg}, title = {Multi-system CAD-teaching in large classes}, series = {When design education and design research meet : proceedings of the 12th International Conference on Engineering and Product Design Education, Norwegian University of Science and Technology (NTNU) Trondheim, Norway, 2nd - 3rd September 2010 ; [E\&PDE]}, booktitle = {When design education and design research meet : proceedings of the 12th International Conference on Engineering and Product Design Education, Norwegian University of Science and Technology (NTNU) Trondheim, Norway, 2nd - 3rd September 2010 ; [E\&PDE]}, publisher = {Design Society}, address = {Glasgow}, organization = {International Conference on Engineering and Product Design Education <12, 2010, Trondheim>}, isbn = {978-1-904670-19-3}, pages = {204 -- 209}, year = {2010}, language = {en} } @inproceedings{GligorevicPulini2010, author = {Gligorevic, Snjezana and Pulini, Paola}, title = {Simplified airport surface channel model based on the WSSUS assumption}, series = {2010 Integrated Communications, Navigation and Surveillance Conference (ICNS 2010) : Herndon, Virginia, USA, 11 - 13 May 2010}, booktitle = {2010 Integrated Communications, Navigation and Surveillance Conference (ICNS 2010) : Herndon, Virginia, USA, 11 - 13 May 2010}, organization = {Institute of Electrical and Electronics Engineers}, isbn = {978-1-4244-7457-8}, issn = {2155-4943}, pages = {F2-1 -- F2-11}, year = {2010}, language = {en} } @inproceedings{SchnellFranzenGligorevic2010, author = {Schnell, Michael and Franzen, Nico and Gligorevic, Snjezana}, title = {L-DACS1 laboratory demonstrator development and compatibility measurement set-up}, series = {IEEE/AIAA 29th Digital Avionics Systems Conference (DASC) : 3 - 7 Oct. 2010, Salt Lake City, Utah}, booktitle = {IEEE/AIAA 29th Digital Avionics Systems Conference (DASC) : 3 - 7 Oct. 2010, Salt Lake City, Utah}, organization = {Institute of Electrical and Electronics Engineers}, isbn = {9781424466160 ; 9781424466184}, pages = {3E3-1 -- 3E3-11}, year = {2010}, language = {en} } @inproceedings{WalterGligorevicDetertetal.2010, author = {Walter, Michael and Gligorevic, Snjezana and Detert, Thorben and Schnell, Michael}, title = {UHF/VHF air-to-air propagation measurements}, series = {Proceedings of the Fourth European Conference on Antennas and Propagation (EuCAP) : 12 - 16 April 2010, Barcelona, Spain}, booktitle = {Proceedings of the Fourth European Conference on Antennas and Propagation (EuCAP) : 12 - 16 April 2010, Barcelona, Spain}, organization = {European Association on Antennas and Propagation}, isbn = {978-1-4244-6431-9 ; 978-84-7653-472-4}, pages = {1 -- 5}, year = {2010}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} }