@article{BohrnStuetzFuchsetal.2012, author = {Bohrn, U. and St{\"u}tz, E. and Fuchs, K. and Fleischer, M. and Sch{\"o}ning, Michael Josef and Wagner, P.}, title = {Monitoring of irritant gas using a whole-cell-based sensor system}, series = {Sensors and Actuators B: Chemical}, volume = {175}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2012.05.088}, pages = {208 -- 217}, year = {2012}, abstract = {Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas®), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security.}, language = {en} } @article{SchneiderSchneider2012, author = {Schneider, Bettina and Schneider, Wilhelm}, title = {Due Diligence - Grundlagen und Checkliste f{\"u}r KMU}, series = {Betriebswirtschaft im Blickpunkt}, journal = {Betriebswirtschaft im Blickpunkt}, publisher = {IWW Institut f{\"u}r Wissen in der Wirtschaft}, address = {Nordkirchen}, issn = {2191-1118}, pages = {148 -- 149}, year = {2012}, abstract = {Im Folgenden sollen Anlass und Inhalt einer Due Diligence erl{\"a}utert werden. Im Rahmen einer Checkliste werden Beratern und Unternehmen Hinweise zu m{\"o}glichen Pr{\"u}fungsgebieten sowie zu vorzuhaltenden oder zu pr{\"u}fenden Unterlagen gegeben. Entstanden ist die Checkliste aus der langj{\"a}hrigen praktischen T{\"a}tigkeit der Autoren bei einer großen Wirtschaftspr{\"u}fungsgesellschaft bzw. einer deutschen Großbank.}, language = {de} } @book{WosnitzaHilgers2012, author = {Wosnitza, Franz and Hilgers, Hans Gerd}, title = {Energieeffizienz und Energiemanagement : Ein {\"U}berblick heutiger M{\"o}glichkeiten und Notwendigkeiten}, publisher = {Vieweg + Teubner}, address = {Wiesbaden}, isbn = {978-3-8348-8671-2}, pages = {Elektronische Ressource}, year = {2012}, language = {de} } @incollection{Laack2012, author = {Laack, Walter van}, title = {Elektro- und Ultraschalltherapie}, series = {Leitfaden Naturheilverfahren : f{\"u}r die {\"a}rztliche Praxis}, booktitle = {Leitfaden Naturheilverfahren : f{\"u}r die {\"a}rztliche Praxis}, editor = {Beer, Andr{\´e}-Michael and Adler, Martin}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {978-3-437-56103-0}, doi = {10.1016/B978-3-437-56103-0.10013-5}, pages = {285 -- 299}, year = {2012}, language = {de} } @book{Laack2012, author = {Laack, Walter van}, title = {Schnittstelle Tod: Warum auf ein Danach vertrauen?}, publisher = {Books on Demand}, address = {Norderstedt}, isbn = {978-3-936624-14-4}, pages = {120 S. : Ill.,graph. Darst.}, year = {2012}, language = {de} } @article{BleckenHolthausSellheier2012, author = {Blecken, Udo and Holthaus-Sellheier, Ursula}, title = {Quantitative Prognoseverfahren f{\"u}r Mietpreise von Bueroimmobilien am Beispiel D{\"u}sseldorf}, series = {GuG : Grundst{\"u}cksmarkt und Grundst{\"u}ckswert}, volume = {23}, journal = {GuG : Grundst{\"u}cksmarkt und Grundst{\"u}ckswert}, number = {1}, publisher = {Werner}, address = {K{\"o}ln}, issn = {0938-0175}, pages = {12 -- 21}, year = {2012}, language = {de} } @article{KurulganDemirciDemirciLinderetal.2012, author = {Kurulgan Demirci, Eylem and Demirci, Taylan and Linder, Peter and Trzewik, J{\"u}rgen and Gierkowski, Jessica Ricarda and Gossmann, Matthias and Kayser, Peter and Porst, Dariusz and Digel, Ilya and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells}, series = {Journal of Bioscience and Bioengineering}, volume = {113}, journal = {Journal of Bioscience and Bioengineering}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1347-4421}, doi = {10.1016/j.jbiosc.2012.03.019}, pages = {212 -- 219}, year = {2012}, abstract = {All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.}, language = {en} } @article{KlingeWilkeMuehl2012, author = {Klinge, Uwe and Wilke, Ralf and M{\"u}hl, Thomas}, title = {Auswahl der Herniennetze nach der "effektiven Porosit{\"a}t" - warum das Gewicht weniger wichtig ist}, series = {Chirurgische Allgemeine : CHAZ ; Zeitung f{\"u}r Klinik und Praxis}, volume = {13}, journal = {Chirurgische Allgemeine : CHAZ ; Zeitung f{\"u}r Klinik und Praxis}, number = {12}, publisher = {Kaden}, address = {Mannheim}, isbn = {1615-5378}, pages = {297 -- 301}, year = {2012}, language = {de} } @article{WernerGroebelKrumbeetal.2012, author = {Werner, Frederik and Groebel, Simone and Krumbe, Christoph and Wagner, Torsten and Selmer, Thorsten and Yoshinobu, Tatsuo and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {Nutrient concentration-sensitive microorganism-based biosensor}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100801}, pages = {900 -- 904}, year = {2012}, language = {en} } @article{KirchnerReisertPuetzetal.2012, author = {Kirchner, Patrick and Reisert, Steffen and P{\"u}tz, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Characterisation of polymeric materials as passivation layer for calorimetric H2O2 gas sensors}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100773}, pages = {859 -- 863}, year = {2012}, abstract = {Calorimetric gas sensors for monitoring the H₂O₂ concentration at elevated temperatures in industrial sterilisation processes have been presented in previous works. These sensors are built up in form of a differential set-up of a catalytically active and passive temperature-sensitive structure. Although, various types of catalytically active dispersions have been studied, the passivation layer has to be established and therefore, chemically as well as physically characterised. In the present work, fluorinated ethylene propylene (FEP), perfluoralkoxy (PFA) and epoxy-based SU-8 photoresist as temperature-stable polymeric materials have been investigated for sensor passivation in terms of their chemical inertness against H₂O₂, their hygroscopic properties as well as their morphology. The polymeric materials were deposited via spin-coating on the temperature-sensitive structure, wherein spin-coated FEP and PFA show slight agglomerates. However, they possess a low absorption of humidity due to their hydrophobic surface, whereas the SU-8 layer has a closed surface but shows a slightly higher absorption of water. All of them were inert against gaseous H₂O₂ during the characterisation in H₂O₂ atmosphere that demonstrates their suitability as passivation layer for calorimetric H₂O₂ gas sensors.}, language = {en} }