@article{WeldenPoghossianVahidpouretal.2022, author = {Welden, Melanie and Poghossian, Arshak and Vahidpour, Farnoosh and Wendlandt, Tim and Keusgen, Michael and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers}, series = {Biosensors}, volume = {12}, journal = {Biosensors}, number = {1}, publisher = {MDPI}, address = {Basel}, issn = {2079-6374}, doi = {10.3390/bios12010043}, pages = {Artikel 43}, year = {2022}, abstract = {Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1-3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.}, language = {en} } @inproceedings{WeilPoghossianSchoeningetal.2012, author = {Weil, M. and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Cherstvy, A.}, title = {Electrical monitoring of layer-by-layer adsorption of oppositely charged macromolecules by means of capacitive field-effect devices}, isbn = {978-3-9813484-2-2}, doi = {10.5162/IMCS2012/P2.5.2}, pages = {1575 -- 1578}, year = {2012}, language = {en} } @article{WarmerWagnerSchoeningetal.2015, author = {Warmer, Johannes and Wagner, Patrick and Sch{\"o}ning, Michael Josef and Kaul, Peter}, title = {Detection of triacetone triperoxide using temperature cycled metal-oxide semiconductor gas sensors}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431882}, pages = {1289 -- 1298}, year = {2015}, language = {en} } @article{WangKrauseBlocketal.2003, author = {Wang, J. and Krause, R. and Block, K. and Musameh, M. and Mulchandani, A. and Sch{\"o}ning, Michael Josef}, title = {Flow injection amperometric detection of OP nerve agents based on organophosphorus-hydrolase biosensor detector}, series = {Biosensors \& Bioelectronics. 18 (2003), H. 2-3}, journal = {Biosensors \& Bioelectronics. 18 (2003), H. 2-3}, isbn = {0956-5663}, pages = {255 -- 260}, year = {2003}, language = {en} } @article{WagnerYoshinobuRaoetal.2005, author = {Wagner, Torsten and Yoshinobu, T. and Rao, C. and Otto, R. and Sch{\"o}ning, Michael Josef}, title = {„All-in-one" solid-state device based on a lightaddressable potentiometric sensor platform}, series = {Transducers '05 : the 13th International Conference on Solid-State Sensors, Actuators and Microsystems ; Seoul, Korea, [June 5 - 9, 2005] ; digest of technical papers / sponsored by Korean Sensors Society. Technical co-sponsors: IEEE Electron Devices Society}, journal = {Transducers '05 : the 13th International Conference on Solid-State Sensors, Actuators and Microsystems ; Seoul, Korea, [June 5 - 9, 2005] ; digest of technical papers / sponsored by Korean Sensors Society. Technical co-sponsors: IEEE Electron Devices Society}, publisher = {IEEE Operations Center}, address = {Piscataway, NJ}, isbn = {0-7803-83995-6}, pages = {1872 -- 1875}, year = {2005}, language = {en} } @article{WagnerWernerMiyamotoetal.2012, author = {Wagner, Torsten and Werner, Frederik and Miyamoto, Ko-Ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Development and characterisation of a compact light-addressable potentiometric sensor (LAPS) based on the digital light processing (DLP) technology for flexible chemical imaging}, series = {Sensors and Actuators B: Chemical}, volume = {170}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2010.12.003}, pages = {34 -- 39}, year = {2012}, abstract = {Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach to read out light-addressable potentiometric sensors (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device, which allows fast and flexible generation of light patterns. With the help of these light patterns, the sensor surface of the LAPS device can be addressed. The DLP approach has several advantages compared to conventional LAPS set-ups, e.g., the spot size and the shape of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of the measurement. In addition, the modulation frequency and intensity of the light beam are important parameters of the LAPS set-up. Within this work, the authors will discuss two different ways of light modulation by the DLP set-up, investigate the influence of different modulation frequencies and different light intensities as well as demonstrate the scanning capabilities of the new set-up by pH mapping on the sensor surface.}, language = {en} } @article{WagnerWernerMiyamotoetal.2011, author = {Wagner, Torsten and Werner, Frederik and Miyamoto, Ko-Ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {A high-density multi-point LAPS set-up using a VCSEL array and FPGA control}, series = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, journal = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1873-3077}, pages = {124 -- 128}, year = {2011}, language = {en} } @article{WagnerWernerMiyamotoetal.2010, author = {Wagner, Torsten and Werner, Frederik and Miyamoto, Ko-ichiro and Ackermann, Hans-Josef and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {FPGA-based LAPS device for the flexible design of sensing sites on functional interfaces}, series = {Physica Status Solidi (A). 207 (2010), H. 4}, journal = {Physica Status Solidi (A). 207 (2010), H. 4}, isbn = {1862-6300}, pages = {844 -- 849}, year = {2010}, language = {en} } @article{WagnerWernerMiyamotoetal.2009, author = {Wagner, Torsten and Werner, Frederik and Miyamoto, K. and Sch{\"o}ning, Michael Josef and Yoshinobu, T.}, title = {A high-density multi-point LAPS set-up using a VCSEL array and FPGA control}, series = {Procedia Chemistry. 1 (2009), H. 1}, journal = {Procedia Chemistry. 1 (2009), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1876-6196}, pages = {1483 -- 1486}, year = {2009}, language = {en} } @article{WagnerVornholtWerneretal.2016, author = {Wagner, Torsten and Vornholt, Wolfgang and Werner, Frederik and Yoshinobu, Tatsuo and Miyamoto, Ko-Ichiro and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Light-addressable potentiometric sensor (LAPS) combined with magnetic beads for pharmaceutical screening}, series = {Physics in medicine}, volume = {2016}, journal = {Physics in medicine}, number = {1}, issn = {2352-4510}, doi = {10.1016/j.phmed.2016.03.001}, pages = {2 -- 7}, year = {2016}, abstract = {The light-addressable potentiometric sensor (LAPS) has the unique feature to address different regions of a sensor surface without the need of complex structures. Measurements at different locations on the sensor surface can be performed in a common analyte solution, which distinctly simplifies the fluidic set-up. However, the measurement in a single analyte chamber prevents the application of different drugs or different concentrations of a drug to each measurement spot at the same time as in the case of multi-reservoir-based set-ups. In this work, the authors designed a LAPS-based set-up for cell culture screening that utilises magnetic beads loaded with the endotoxin (lipopolysaccharides, LPS), to generate a spatially distributed gradient of analyte concentration. Different external magnetic fields can be adjusted to move the magnetic beads loaded with a specific drug within the measurement cell. By recording the metabolic activities of a cell layer cultured on top of the LAPS surface, this work shows the possibility to apply different concentrations of a sample along the LAPS measurement spots within a common analyte solution.}, language = {en} }