@article{WeldenSeverinsPoghossianetal.2022,
  author    = {Welden, Melanie and Severins, Robin and Poghossian, Arshak and Wege, Christina and Bongaerts, Johannes and Siegert, Petra and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor},
  series = {Chemosensors},
  volume    = {10},
  journal   = {Chemosensors},
  number    = {6},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2227-9040},
  doi       = {10.3390/chemosensors10060218},
  pages     = {Artikel 218},
  year      = {2022},
  abstract  = {Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte-insulator-semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin-streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage-current, capacitance-voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.},
  language  = {en}
}
@inproceedings{MolinnusHardtKaeveretal.2017,
  author    = {Molinnus, Denise and Hardt, Gabriel and K{\"a}ver, Larissa and Willenberg, Holger S. and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology},
  series = {MDPI Proceedings},
  booktitle = {MDPI Proceedings},
  doi       = {10.3390/proceedings1040506},
  pages     = {4 Seiten},
  year      = {2017},
  language  = {en}
}
@article{MolinnusBaeckerSiegertetal.2015,
  author    = {Molinnus, Denise and B{\"a}cker, Matthias and Siegert, Petra and Willenberg, H. and Poghossian, Arshak and Keusgen, M. and Sch{\"o}ning, Michael Josef},
  title     = {Detection of Adrenaline Based on Substrate Recycling Amplification},
  series = {Procedia Engineering},
  volume    = {120},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2015.08.708},
  pages     = {540 -- 543},
  year      = {2015},
  abstract  = {An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.},
  language  = {en}
}
@article{MolinnusHardtSiegertetal.2018,
  author    = {Molinnus, Denise and Hardt, Gabriel and Siegert, Petra and Willenberg, Holger S. and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Detection of Adrenaline in Blood Plasma as Biomarker for Adrenal Venous Sampling},
  series = {Electroanalysis},
  volume    = {30},
  journal   = {Electroanalysis},
  number    = {5},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1521-4109},
  doi       = {10.1002/elan.201800026},
  pages     = {937 -- 942},
  year      = {2018},
  abstract  = {An amperometric bi-enzyme biosensor based on substrate recycling principle for the amplification of the sensor signal has been developed for the detection of adrenaline in blood. Adrenaline can be used as biomarker verifying successful adrenal venous sampling procedure. The adrenaline biosensor has been realized via modification of a galvanic oxygen sensor with a bi-enzyme membrane combining a genetically modified laccase and a pyrroloquinoline quinone-dependent glucose dehydrogenase. The measurement conditions such as pH value and temperature were optimized to enhance the sensor performance. A high sensitivity and a low detection limit of about 0.5-1 nM adrenaline have been achieved in phosphate buffer at pH 7.4, relevant for measurements in blood samples. The sensitivity of the biosensor to other catecholamines such as noradrenaline, dopamine and dobutamine has been studied. Finally, the sensor has been successfully applied for the detection of adrenaline in human blood plasma.},
  language  = {en}
}
@article{SchoeningPoghossian2008,
  author    = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak},
  title     = {Detection of charged macromolecules by means of field-effect devices (FEDs): possibilities and limitations},
  series = {Electrochemical sensors, biosensors and their biomedical applications / ed. by Xueji Zhang ...},
  journal   = {Electrochemical sensors, biosensors and their biomedical applications / ed. by Xueji Zhang ...},
  publisher = {Elsevier Acad. Press},
  address   = {Amsterdam},
  isbn      = {978-0-12-373738-0},
  pages     = {187 -- 212},
  year      = {2008},
  language  = {en}
}
@inproceedings{NaetherJuarezEmmerichetal.2006,
  author    = {N{\"a}ther, Niko and Ju{\´a}rez, Leon M. and Emmerich, R{\"u}diger and Berger, J{\"o}rg and Friedrich, Peter and Sch{\"o}ning, Michael Josef},
  title     = {Detection of hydrogen peroxide (H2O2) at exposed temperatures for industrial processes},
  url       = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-1418},
  year      = {2006},
  abstract  = {An H2O2 sensor for the application in industrial sterilisation processes has been developed. Therefore, automated sterilisation equipment at laboratory scale has been constructed using parts from industrial sterilisation facilities. In addition, a software tool has been developed for the control of the sterilisation equipment at laboratory scale. First measurements with the developed sensor set-up as part of the sterilisation equipment have been performed and the sensor has been physically characterised by optical microscopy and SEM.},
  subject      = {Biosensor},
  language  = {en}
}
@article{OberlaenderKirchnerBoyenetal.2014,
  author    = {Oberl{\"a}nder, Jan and Kirchner, Patrick and Boyen, Hans-Gerd and Sch{\"o}ning, Michael Josef},
  title     = {Detection of hydrogen peroxide vapor by use of manganese(IV) oxide as catalyst for calorimetric gas sensors},
  series = {Physica status solidi A: Applications and materials science},
  volume    = {211},
  journal   = {Physica status solidi A: Applications and materials science},
  number    = {6},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)},
  doi       = {10.1002/pssa.201330359},
  pages     = {1372 -- 1376},
  year      = {2014},
  abstract  = {In this work, the catalyst manganese(IV) oxide (MnO2), of calorimetric gas sensors (to monitor the sterilization agent vaporized hydrogen peroxide) has been investigated in more detail. Chemical analyses by means of X-ray-induced photoelectron spectroscopy have been performed to unravel the surface chemistry prior and after exposure to hydrogen peroxide vapor at elevated temperature, as applied in the sterilization processes of beverage cartons. The surface characterization reveals a change in oxidation states of the metal oxide catalyst after exposure to hydrogen peroxide. Additionally, a cleaning effect of the catalyst, which itself is attached to the sensor surface by means of a polymer interlayer, could be observed.},
  language  = {en}
}
@article{KassabHanPoghossianetal.2004,
  author    = {Kassab, T. and Han, Y. and Poghossian, Arshak and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef},
  title     = {Detection of layerby-layer adsorbed polyelectrolytes by means of field-effect based capacitive EIS structures},
  series = {Biomedizinische Technik. 49 (2004), H. 2},
  journal   = {Biomedizinische Technik. 49 (2004), H. 2},
  isbn      = {0932-4666},
  pages     = {1034 -- 1035},
  year      = {2004},
  language  = {en}
}
@article{EmonsHuellenkremerSchoening2001,
  author    = {Emons, H. and H{\"u}llenkremer, B. and Sch{\"o}ning, Michael Josef},
  title     = {Detection of metal ions in aqueous solutions by voltohmmetry},
  series = {Fresenius' Journal of Analytical Chemistry. 369 (2001), H. 1},
  journal   = {Fresenius' Journal of Analytical Chemistry. 369 (2001), H. 1},
  isbn      = {0937-0633},
  pages     = {42 -- 46},
  year      = {2001},
  language  = {en}
}
@article{BronderJessingPoghossianetal.2018,
  author    = {Bronder, Thomas and Jessing, Max P. and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Detection of PCR-Amplified Tuberculosis DNA Fragments with Polyelectrolyte-Modified Field-Effect Sensors},
  series = {Analytical Chemistry},
  volume    = {90},
  journal   = {Analytical Chemistry},
  number    = {12},
  publisher = {ACS Publications},
  address   = {Washington, DC},
  issn      = {0003-2700},
  doi       = {10.1021/acs.analchem.8b01807},
  pages     = {7747 -- 7753},
  year      = {2018},
  abstract  = {Field-effect-based electrolyte-insulator-semiconductor (EIS) sensors were modified with a bilayer of positively charged weak polyelectrolyte (poly(allylamine hydrochloride) (PAH)) and probe single-stranded DNA (ssDNA) and are used for the detection of complementary single-stranded target DNA (cDNA) in different test solutions. The sensing mechanism is based on the detection of the intrinsic molecular charge of target cDNA molecules after the hybridization event between cDNA and immobilized probe ssDNA. The test solutions contain synthetic cDNA oligonucleotides (with a sequence of tuberculosis mycobacteria genome) or PCR-amplified DNA (which origins from a template DNA strand that has been extracted from Mycobacterium avium paratuberculosis-spiked human sputum samples), respectively. Sensor responses up to 41 mV have been measured for the test solutions with DNA, while only small signals of ∼5 mV were detected for solutions without DNA. The lower detection limit of the EIS sensors was ∼0.3 nM, and the sensitivity was ∼7.2 mV/decade. Fluorescence experiments using SybrGreen I fluorescence dye support the electrochemical results.},
  language  = {en}
}