@article{HagerHentschkeHojdisetal.2015, author = {Hager, Jonathan and Hentschke, Reinhard and Hojdis, Nils and Karimi-Varzaneh, Hossein Ali}, title = {Computer Simulation of Particle-Particle Interaction in a Model Polymer Nanocomposite}, series = {Macromolecules}, volume = {48}, journal = {Macromolecules}, number = {24}, issn = {1520-5835}, doi = {10.1021/acs.macromol.5b01864}, pages = {9039 -- 9049}, year = {2015}, language = {en} } @article{BaeckerBegingBisellietal.2009, author = {B{\"a}cker, Matthias and Beging, Stefan and Biselli, Manfred and Poghossian, Arshak and Wang, J. and Zang, Werner and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Concept for a solid-state multi-parameter sensor system for cell-culture monitoring}, series = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, journal = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0013-4686}, pages = {6107 -- 6112}, year = {2009}, language = {en} } @article{HaegerGrankinWagner2023, author = {Haeger, Gerrit and Grankin, Alina and Wagner, Michaela}, title = {Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology}, series = {Applied Research}, journal = {Applied Research}, number = {Early View}, publisher = {Wiley-VCH}, issn = {2702-4288}, doi = {10.1002/appl.202200106}, pages = {1 -- 15}, year = {2023}, abstract = {Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47\%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78\%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.}, language = {en} } @article{Scherer2006, author = {Scherer, Ulrich W.}, title = {Controlled ion track etching / J. George; M. Irkens ; S. Neumann ; U. W. Scherer ; A. Srivastava ; D. Sinha ; D. Fink}, series = {Radiation Effects and Defects in Solids. 161 (2006), H. 3}, journal = {Radiation Effects and Defects in Solids. 161 (2006), H. 3}, pages = {161 -- 175}, year = {2006}, language = {en} } @article{SelmerLukatelaKraussetal.1998, author = {Selmer, Thorsten and Lukatela, G. and Krauss, N. and Theis, K.}, title = {Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis / Lukatela, G. ; Krauss, N. ; Theis, K. ; Selmer, T. ; Gieselmann, V. ; Figura, K. von ; Saenger,}, series = {Biochemistry. 37 (1998), H. 11}, journal = {Biochemistry. 37 (1998), H. 11}, pages = {3654 -- 3664}, year = {1998}, language = {en} } @article{HemmerlingMerschenzQuackWunderlich1988, author = {Hemmerling, H.-J. and Merschenz-Quack, Angelika and Wunderlich, H.}, title = {Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988}, series = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, volume = {185}, journal = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, number = {H. 1-4}, issn = {2196-7105 (E-Books); 2194-4946 (Print)}, pages = {256}, year = {1988}, language = {en} } @article{LauthHoelderichWagenblast1995, author = {Lauth, Jakob and Hoelderich, W. and Wagenblast, G.}, title = {Crystalline zeolites containing indigo dyes}, series = {Zeolites. 15 (1995), H. 1}, journal = {Zeolites. 15 (1995), H. 1}, isbn = {0144-2449}, pages = {86}, year = {1995}, language = {en} } @article{BiselliNollJelineketal.2002, author = {Biselli, Manfred and Noll, Thomas and Jelinek, Nanni and Schmidt, Sebastian}, title = {Cultivation of Hematopoietic Stem and Progenitor Cells: biochemical Engineering Aspects / Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli und Christian Wandrey}, series = {Tools and Applications of Biochemical Engineering Science}, journal = {Tools and Applications of Biochemical Engineering Science}, publisher = {Springer}, address = {Berlin}, isbn = {3-540-42250-1}, pages = {111 -- 128}, year = {2002}, language = {en} } @article{BiselliHilbertNoll2001, author = {Biselli, Manfred and Hilbert, U. and Noll, T.}, title = {Cultivation of Human HCMV Specific Lymphocytes - An Example for Adoptive Immunotherapy / Hilbert, U. ; Biselli, M. ; Noll, T.}, series = {Animal cell technology : from target to market ; Tyl{\"o}sand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...}, journal = {Animal cell technology : from target to market ; Tyl{\"o}sand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...}, publisher = {Kluwer}, address = {Dordrecht}, isbn = {1-4020-0264-5}, pages = {558 -- 561}, year = {2001}, language = {en} } @article{Schnitzler2009, author = {Schnitzler, Thomas}, title = {Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)}, series = {Application notes / Sartorius stedim biotech}, journal = {Application notes / Sartorius stedim biotech}, pages = {1 -- 4}, year = {2009}, language = {en} }