@article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @misc{Alt2010, author = {Alt, Helmut}, title = {Optionen einer bezahlbaren Energieversorgung „Vision versus Realismus" : Beitrag f{\"u}r KTG Kongress: vom 4. bis 6. Mai 2010, Berlin}, pages = {1 -- 10}, year = {2010}, language = {de} } @book{Siedenbiedel2010, author = {Siedenbiedel, Georg}, title = {Organisation}, publisher = {Lucius \& Lucius}, address = {Stuttgart}, isbn = {978-3-8252-8413-8}, pages = {XXVI, 546 S. : Ill., graph. Darst.}, year = {2010}, language = {de} } @book{SchulteZurhausen2010, author = {Schulte-Zurhausen, Manfred}, title = {Organisation / 5., {\"u}berarb. und aktualisierte Aufl.}, publisher = {Vahlen}, address = {M{\"u}nchen}, isbn = {978-3-8006-3736-2}, pages = {XVI, 639 S. : graph. Darst.}, year = {2010}, language = {de} } @article{TippkoetterRoikaewUlberetal.2010, author = {Tippk{\"o}tter, Nils and Roikaew, Wipa and Ulber, Roland and Hoffmann, Alexander and Denzler, Hans-J{\"o}rg and Buchholz, Heinrich}, title = {Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food}, series = {Biotechnology Progress}, volume = {26}, journal = {Biotechnology Progress}, number = {3}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {8756-7938}, doi = {10.1002/btpr.384}, pages = {756 -- 762}, year = {2010}, abstract = {Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed.}, language = {en} } @inproceedings{MatchaLjubas2010, author = {Matcha, Heike and Ljubas, Ante}, title = {Parametric Origami: Adaptable temporary buildings}, series = {Future cities: 28th eCAADe Conference Proceedings. eCAADe: Conferences. Zurich, Switzerland}, booktitle = {Future cities: 28th eCAADe Conference Proceedings. eCAADe: Conferences. Zurich, Switzerland}, isbn = {978-0-9541183-7-2}, pages = {243 -- 251}, year = {2010}, language = {en} } @article{BuniatyanAbouzarMartirosyanetal.2010, author = {Buniatyan, Vahe V. and Abouzar, Maryam H. and Martirosyan, Norayr W. and Schubert, J{\"u}rgen and Gevorgian, Spartak and Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {pH-sensitive properties of barium strontium titanate (BST) thin films prepared by pulsed laser deposition technique}, series = {Physica Status Solidi (A). 207 (2010), H. 4}, journal = {Physica Status Solidi (A). 207 (2010), H. 4}, isbn = {1862-6300}, pages = {824 -- 830}, year = {2010}, language = {en} } @article{FleischhakerEversDey2010, author = {Fleischhaker, Robert and Evers, J{\"o}rg and Dey, Tarak N.}, title = {Phase modulation induced by cooperative effects in electromagnetically induced transparency}, series = {Physical Review A - Atomic, molecular, and optical physics}, volume = {82}, journal = {Physical Review A - Atomic, molecular, and optical physics}, number = {1}, publisher = {AIP Publishing}, address = {Melville, NY}, issn = {1050-2947}, doi = {10.1103/PhysRevA.82.013815}, pages = {013815}, year = {2010}, abstract = {We analyze the influence of dipole-dipole interactions in an electromagnetically induced transparency set up for a density at the onset of cooperative effects. To this end, we include mean-field models for the influence of local-field corrections and radiation trapping into our calculation. We show both analytically and numerically that the polarization contribution to the local field strongly modulates the phase of a weak pulse. We give an intuitive explanation for this local-field-induced phase modulation and demonstrate that it distinctively differs from the nonlinear self-phase-modulation that a strong pulse experiences in a Kerr medium.}, language = {en} } @inproceedings{KerpenBungSchlurmann2010, author = {Kerpen, N. B. and Bung, Daniel B. and Schlurmann, Torsten}, title = {Physical model investigations of pressure distributions next to ships passing through a lock}, series = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, booktitle = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010}, publisher = {Univ. Press}, address = {Tianjin}, organization = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <5, 2010, Tianjin>}, isbn = {978-7-5618-3671-2}, pages = {514 -- 519}, year = {2010}, language = {en} } @inproceedings{KerpenBungSchlurmann2010, author = {Kerpen, N. B. and Bung, Daniel B. and Schlurmann, Torsten}, title = {Physical model investigations of ships passing through a lock}, series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, editor = {Janssen, Robert}, publisher = {School of Civil Engineeering, The University of Queensland}, address = {Brisbane}, organization = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>}, isbn = {9781742720159}, pages = {93 -- 100}, year = {2010}, language = {en} }