@article{BienerSasse1993, author = {Biener, Ernst and Sasse, T.}, title = {Construction and rehabilitation of landfill shafts}, series = {Sardinia 93 : Fourth International Landfill Symposium "Barrier systems, environmental aspects, upgrading and remediation, siting, monofills, effects of waste pretreatment, landfilling in developing countries" ; 11 - 15 October 1993, S. Margherita di Pula (Cagliari), Sardinia, Italy ; proceedings / coord. by Thomas H. Christensen ... - Bd. 1}, journal = {Sardinia 93 : Fourth International Landfill Symposium "Barrier systems, environmental aspects, upgrading and remediation, siting, monofills, effects of waste pretreatment, landfilling in developing countries" ; 11 - 15 October 1993, S. Margherita di Pula (Cagliari), Sardinia, Italy ; proceedings / coord. by Thomas H. Christensen ... - Bd. 1}, publisher = {CISA, Environmental Sanitary Engineering Centre}, address = {Cagliari}, pages = {451 -- 460}, year = {1993}, language = {en} } @article{HaegerGrankinWagner2023, author = {Haeger, Gerrit and Grankin, Alina and Wagner, Michaela}, title = {Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology}, series = {Applied Research}, journal = {Applied Research}, number = {Early View}, publisher = {Wiley-VCH}, issn = {2702-4288}, doi = {10.1002/appl.202200106}, pages = {1 -- 15}, year = {2023}, abstract = {Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47\%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78\%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.}, language = {en} } @article{BienerSasse1994, author = {Biener, Ernst and Sasse, T.}, title = {Construction of composite lining systems under unfavourable weather conditions}, series = {Landfilling of waste: barriers / ed. by T. H. Christensen; R. Cossu; R. Stegmann. - 1. Aufl.}, journal = {Landfilling of waste: barriers / ed. by T. H. Christensen; R. Cossu; R. Stegmann. - 1. Aufl.}, publisher = {Spon}, address = {London [u.a.]}, isbn = {0419159908}, pages = {183 -- 200}, year = {1994}, language = {en} } @article{Wrede2016, author = {Wrede, Oliver}, title = {Continuity in travel information}, series = {Information Design Journal}, volume = {22}, journal = {Information Design Journal}, number = {2}, publisher = {John Benjamins}, address = {Amsterdam}, issn = {0142-5471}, doi = {10.1075/idj.22.2.09wre}, pages = {172 -- 178}, year = {2016}, abstract = {This article discusses the contrast between the information transportation companies provide to travellers and that of their brand messaging. Companies' brand messaging often portrays the service they provide as pleasant, stress free and perfect. Customers and users of the service, on the other hand, often describe their experience of the service as a negative one. This article suggests that the brand value would be greater if transportation companies paid more attention to the users' experience when designing their information systems, particularly in worst case scenarios.}, language = {en} } @inproceedings{PaulsenHoffstadtKrafftetal.2020, author = {Paulsen, Svea and Hoffstadt, Kevin and Krafft, Simone and Leite, A. and Zang, J. and Fonseca-Zang, W. and Kuperjans, Isabel}, title = {Continuous biogas production from sugarcane as sole substrate}, series = {Energy Reports}, volume = {6}, booktitle = {Energy Reports}, number = {Supplement 1}, publisher = {Elsevier}, doi = {10.1016/j.egyr.2019.08.035}, pages = {153 -- 158}, year = {2020}, language = {en} } @inproceedings{PetersZiemonsDammersetal.2001, author = {Peters, H. and Ziemons, Karl and Dammers, J. and M{\"u}ller-Veggian, Mattea}, title = {Continuous head motion detection during MEG measurement using head location coils}, series = {Proceedings of the 12th International Conference on Biomagnetism : August 13 - 17, 2000, Helsinki University of Technology, Espoo, Finland}, booktitle = {Proceedings of the 12th International Conference on Biomagnetism : August 13 - 17, 2000, Helsinki University of Technology, Espoo, Finland}, editor = {Nenonen, Jukka}, publisher = {Helsinki Univ. of Technology, Laboratory of Biomedical Engineering}, address = {Espoo}, isbn = {951-22-5401-8}, pages = {XX, 1060 S. : Ill., graph. Darst.}, year = {2001}, language = {en} } @inproceedings{SchmidtsKraftSchreiberetal.2018, author = {Schmidts, Oliver and Kraft, Bodo and Schreiber, Marc and Z{\"u}ndorf, Albert}, title = {Continuously evaluated research projects in collaborative decoupled environments}, series = {2018 ACM/IEEE 5th International Workshop on Software Engineering Research and Industrial PracticePractice, May 29, 2018, Gothenburg, Sweden : SER\&IP' 18}, booktitle = {2018 ACM/IEEE 5th International Workshop on Software Engineering Research and Industrial PracticePractice, May 29, 2018, Gothenburg, Sweden : SER\&IP' 18}, publisher = {ACM}, address = {New York, NY}, pages = {1 -- 9}, year = {2018}, abstract = {Often, research results from collaboration projects are not transferred into productive environments even though approaches are proven to work in demonstration prototypes. These demonstration prototypes are usually too fragile and error-prone to be transferred easily into productive environments. A lot of additional work is required. Inspired by the idea of an incremental delivery process, we introduce an architecture pattern, which combines the approach of Metrics Driven Research Collaboration with microservices for the ease of integration. It enables keeping track of project goals over the course of the collaboration while every party may focus on their expert skills: researchers may focus on complex algorithms, practitioners may focus on their business goals. Through the simplified integration (intermediate) research results can be introduced into a productive environment which enables getting an early user feedback and allows for the early evaluation of different approaches. The practitioners' business model benefits throughout the full project duration.}, language = {en} } @article{KurzLinderTrzewiketal.2010, author = {Kurz, R. and Linder, Peter and Trzewik, J{\"u}rgen and R{\"u}ffer, M. and Artmann, Gerhard and Digel, Ilya and Rothermel, A. and Robitzki, A. and Temiz Artmann, Ayseg{\"u}l}, title = {Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes}, series = {Medical and Biological Engineering and Computing}, volume = {48}, journal = {Medical and Biological Engineering and Computing}, number = {1}, publisher = {Springer Nature}, address = {Cham}, issn = {1741-0444}, doi = {10.1007/s11517-009-0552-y}, pages = {59 -- 65}, year = {2010}, abstract = {The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.}, language = {en} } @article{KurulganDemirciLinderDemircietal.2009, author = {Kurulgan Demirci, Eylem and Linder, Peter and Demirci, Taylan and Trzewik, J{\"u}rgen and Digel, Ilya and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Contractile tension of endothelial cells: An LPS based in-vitro sepsis model}, series = {IUBMB Life. 61 (2009), H. 3}, journal = {IUBMB Life. 61 (2009), H. 3}, publisher = {Wiley}, address = {Weinheim}, isbn = {1521-6543}, pages = {307 -- 308}, year = {2009}, language = {en} } @article{UmutluBitzMaderwaldetal.2013, author = {Umutlu, Lale and Bitz, Andreas and Maderwald, Stefan and Orzada, Stephan and Kinner, Sonja and Kraff, Oliver and Brote, Irina and Ladd, Susanne C. and Schroeder, Tobias and Forsting, Michael}, title = {Contrast-enhanced ultra-high-field liver MRI: a feasibility trial}, series = {European Journal of Radiology}, volume = {82}, journal = {European Journal of Radiology}, number = {5}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0720-048X}, doi = {10.1016/j.ejrad.2011.07.004}, pages = {760 -- 767}, year = {2013}, language = {en} }