@article{ThiebesKleinZingsheimetal.2022, author = {Thiebes, Anja Lena and Klein, Sarah and Zingsheim, Jonas and M{\"o}ller, Georg H. and G{\"u}rzing, Stefanie and Reddemann, Manuel A. and Behbahani, Mehdi and Cornelissen, Christian G.}, title = {Effervescent atomizer as novel cell spray technology to decrease the gas-to-liquid ratio}, series = {pharmaceutics}, volume = {14}, journal = {pharmaceutics}, number = {11}, publisher = {MDPI}, address = {Basel}, doi = {10.3390/pharmaceutics14112421}, pages = {Artikel 2421}, year = {2022}, abstract = {Cell spraying has become a feasible application method for cell therapy and tissue engineering approaches. Different devices have been used with varying success. Often, twin-fluid atomizers are used, which require a high gas velocity for optimal aerosolization characteristics. To decrease the amount and velocity of required air, a custom-made atomizer was designed based on the effervescent principle. Different designs were evaluated regarding spray characteristics and their influence on human adipose-derived mesenchymal stromal cells. The arithmetic mean diameters of the droplets were 15.4-33.5 µm with decreasing diameters for increasing gas-to-liquid ratios. The survival rate was >90\% of the control for the lowest gas-to-liquid ratio. For higher ratios, cell survival decreased to approximately 50\%. Further experiments were performed with the design, which had shown the highest survival rates. After seven days, no significant differences in metabolic activity were observed. The apoptosis rates were not influenced by aerosolization, while high gas-to-liquid ratios caused increased necrosis levels. Tri-lineage differentiation potential into adipocytes, chondrocytes, and osteoblasts was not negatively influenced by aerosolization. Thus, the effervescent aerosolization principle was proven suitable for cell applications requiring reduced amounts of supplied air. This is the first time an effervescent atomizer was used for cell processing.}, language = {en} } @article{HerssensCowburnAlbrachtetal.2022, author = {Herssens, Nolan and Cowburn, James and Albracht, Kirsten and Braunstein, Bjoern and Cazzola, Dario and Colyer, Steffi and Minetti, Alberto E. and Pavei, Gaspare and Rittweger, J{\"o}rn and Weber, Tobias and Green, David A.}, title = {Movement in low gravity environments (MoLo) programme - the MoLo-L.O.O.P. study protocol}, series = {PLOS ONE / Public Library of Science}, volume = {17}, journal = {PLOS ONE / Public Library of Science}, number = {11}, editor = {Cattaneo, Luigi}, publisher = {Plos}, address = {San Francisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0278051}, pages = {e0278051}, year = {2022}, abstract = {Exposure to prolonged periods in microgravity is associated with deconditioning of the musculoskeletal system due to chronic changes in mechanical stimulation. Given astronauts will operate on the Lunar surface for extended periods of time, it is critical to quantify both external (e.g., ground reaction forces) and internal (e.g., joint reaction forces) loads of relevant movements performed during Lunar missions. Such knowledge is key to predict musculoskeletal deconditioning and determine appropriate exercise countermeasures associated with extended exposure to hypogravity.}, language = {en} } @article{MeyerGranrathFeyerletal.2021, author = {Meyer, Max-Arno and Granrath, Christian and Feyerl, G{\"u}nter and Richenhagen, Johannes and Kaths, Jakob and Andert, Jakob}, title = {Closed-loop platoon simulation with cooperative intelligent transportation systems based on vehicle-to-X communication}, series = {Simulation Modelling Practice and Theory}, volume = {106}, journal = {Simulation Modelling Practice and Theory}, number = {Art. 102173}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1569-190X}, doi = {10.1016/j.simpat.2020.102173}, year = {2021}, language = {en} } @article{SerrorHackHenzeetal.2021, author = {Serror, Martin and Hack, Sacha and Henze, Martin and Schuba, Marko and Wehrle, Klaus}, title = {Challenges and Opportunities in Securing the Industrial Internet of Things}, series = {IEEE Transactions on Industrial Informatics}, volume = {17}, journal = {IEEE Transactions on Industrial Informatics}, number = {5}, publisher = {IEEE}, address = {New York}, issn = {1941-0050}, doi = {10.1109/TII.2020.3023507}, pages = {2985 -- 2996}, year = {2021}, language = {en} } @article{TranStaat2021, author = {Tran, Ngoc Trinh and Staat, Manfred}, title = {Direct plastic structural design under random strength and random load by chance constrained programming}, series = {European Journal of Mechanics - A/Solids}, volume = {85}, journal = {European Journal of Mechanics - A/Solids}, number = {Article 104106}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0997-7538}, doi = {10.1016/j.euromechsol.2020.104106}, year = {2021}, language = {en} } @article{SeefeldtDachwald2021, author = {Seefeldt, Patric and Dachwald, Bernd}, title = {Temperature increase on folded solar sail membranes}, series = {Advances in Space Research}, volume = {67}, journal = {Advances in Space Research}, number = {9}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0273-1177}, doi = {10.1016/j.asr.2020.09.026}, pages = {2688 -- 2695}, year = {2021}, language = {en} } @article{AlexyukBogoyavlenskiyAlexyuketal.2021, author = {Alexyuk, Madina and Bogoyavlenskiy, Andrey and Alexyuk, Pavel and Moldakhanov, Yergali and Berezin, Vladimir and Digel, Ilya}, title = {Epipelagic microbiome of the Small Aral Sea: Metagenomic structure and ecological diversity}, series = {MicrobiologyOpen}, volume = {10}, journal = {MicrobiologyOpen}, number = {1}, publisher = {Wiley}, address = {Weinheim}, issn = {2045-8827}, doi = {10.1002/mbo3.1142}, pages = {1 -- 10}, year = {2021}, abstract = {Microbial diversity studies regarding the aquatic communities that experienced or are experiencing environmental problems are essential for the comprehension of the remediation dynamics. In this pilot study, we present data on the phylogenetic and ecological structure of microorganisms from epipelagic water samples collected in the Small Aral Sea (SAS). The raw data were generated by massive parallel sequencing using the shotgun approach. As expected, most of the identified DNA sequences belonged to Terrabacteria and Actinobacteria (40\% and 37\% of the total reads, respectively). The occurrence of Deinococcus-Thermus, Armatimonadetes, Chloroflexi in the epipelagic SAS waters was less anticipated. Surprising was also the detection of sequences, which are characteristic for strict anaerobes—Ignavibacteria, hydrogen-oxidizing bacteria, and archaeal methanogenic species. We suppose that the observed very broad range of phylogenetic and ecological features displayed by the SAS reads demonstrates a more intensive mixing of water masses originating from diverse ecological niches of the Aral-Syr Darya River basin than presumed before.}, language = {en} } @article{HeinkeKnickerAlbracht2021, author = {Heinke, Lars N. and Knicker, Axel J. and Albracht, Kirsten}, title = {Test-retest reliability of the internal shoulder rotator muscles' stretch reflex in healthy men}, series = {Journal of Electromyography and Kinesiology}, volume = {62}, journal = {Journal of Electromyography and Kinesiology}, number = {Article 102611}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1050-6411}, doi = {10.1016/j.jelekin.2021.102611}, year = {2021}, abstract = {Until now the reproducibility of the short latency stretch reflex of the internal rotator muscles of the glenohumeral joint has not been identified. Twenty-three healthy male participants performed three sets of external shoulder rotation stretches with various pre-activation levels on two different dates of measurement to assess test-retest reliability. All stretches were applied with a dynamometer acceleration of 104°/s2 and a velocity of 150°/s. Electromyographical response was measured via surface EMG. Reflex latencies showed a pre-activation effect (ƞ2 = 0,355). ICC ranged from 0,735 to 0,909 indicating an overall "good" relative reliability. SRD 95\% lay between ±7,0 to ±12,3 ms.. The reflex gain showed overall poor test-retest reproducibility. The chosen methodological approach presented a suitable test protocol for shoulder muscles stretch reflex latency evaluation. A proof-of-concept study to validate the presented methodical approach in shoulder involvement including subjects with clinically relevant conditions is recommended.}, language = {en} } @article{TemizArtmannKurulgandemirciFıratetal.2021, author = {Temiz Artmann, Ayseg{\"u}l and Kurulgan demirci, Eylem and F{\i}rat, Ipek Seda and Oflaz, Hakan and Artmann, Gerhard}, title = {Recombinant activated protein C (rhAPC) affects lipopolysaccharide-induced mechanical compliance changes and beat frequency of mESC-derived cardiomyocyte monolayers}, series = {SHOCK}, journal = {SHOCK}, publisher = {Wolters Kluwer}, address = {K{\"o}ln}, issn = {1540-0514}, doi = {10.1097/SHK.0000000000001845}, year = {2021}, language = {en} } @article{HunkerGossmannRamanetal.2021, author = {Hunker, Jan L. and Gossmann, Matthias and Raman, Aravind Hariharan and Linder, Peter}, title = {Artificial neural networks in cardiac safety assessment: Classification of chemotherapeutic compound effects on hiPSC-derived cardiomyocyte contractility}, series = {Journal of Pharmacological and Toxicological Methods}, volume = {111}, journal = {Journal of Pharmacological and Toxicological Methods}, number = {Article number 107044}, publisher = {Elsevier}, address = {New York}, issn = {1056-8719}, doi = {10.1016/j.vascn.2021.107044}, year = {2021}, language = {en} }