@article{NguyenDuongTranetal.2012, author = {Nguyen, Nhu Huynh and Duong, Minh Tuan and Tran, Thanh Ngoc and Pham, Phu Tinh and Grottke, O. and Tolba, R. and Staat, Manfred}, title = {Influence of a freeze-thaw cycle on the stress-stretch curves of tissues of porcine abdominal organs}, series = {Journal of Biomechanics}, volume = {45}, journal = {Journal of Biomechanics}, number = {14}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-2380}, doi = {10.1016/j.jbiomech.2012.07.008}, pages = {2382 -- 2386}, year = {2012}, abstract = {The paper investigates both fresh porcine spleen and liver and the possible decomposition of these organs under a freeze-thaw cycle. The effect of tissue preservation condition is an important factor which should be taken into account for protracted biomechanical tests. In this work, tension tests were conducted for a large number of tissue specimens from twenty pigs divided into two groups of 10. Concretely, the first group was tested in fresh state; the other one was tested after a freeze-thaw cycle which simulates the conservation conditions before biomechanical experiments. A modified Fung model for isotropic behavior was adopted for the curve fitting of each kind of tissues. Experimental results show strong effects of the realistic freeze-thaw cycle on the capsule of elastin-rich spleen but negligible effects on the liver which virtually contains no elastin. This different behavior could be explained by the autolysis of elastin by elastolytic enzymes during the warmer period after thawing. Realistic biomechanical properties of elastin-rich organs can only be expected if really fresh tissue is tested. The observations are supported by tests of intestines.}, language = {en} } @article{KotliarLanzl2011, author = {Kotliar, Konstantin and Lanzl, Ines M.}, title = {Can vascular function be assessed by the interpretation of retinal vascular diameter changes?}, series = {Investigative Ophthalmology \& Visual Science, IOVS. 52 (2011), H. 1}, journal = {Investigative Ophthalmology \& Visual Science, IOVS. 52 (2011), H. 1}, publisher = {ARVO}, address = {Rockville, Md.}, isbn = {0146-0404}, pages = {635 -- 636}, year = {2011}, language = {en} } @article{KotliarMueckeVilseretal.2008, author = {Kotliar, Konstantin and M{\"u}cke, Bruno and Vilser, Walthard and Schilling, Rudolf}, title = {Effect of aging on retinal artery blood column diameter measured along the vessel axis / Kotliar, Konstantin E. ; M{\"u}cke, Bruno ; Vilser, Walthard ; Schilling, Rudolf ; Lanzl, Ines M.}, series = {Investigative Ophthalmology \& Visual Science, IOVS. 49 (2008), H. 5}, journal = {Investigative Ophthalmology \& Visual Science, IOVS. 49 (2008), H. 5}, publisher = {-}, isbn = {0146-0404}, pages = {2094 -- 2102}, year = {2008}, language = {en} } @article{KotliarLanzlHanssenetal.2012, author = {Kotliar, Konstantin and Lanzl, Ines M. and Hanssen, Henner and Eberhardt, Karla and Vilser, Walthard and Halle, Martin and Heemann, Uwe and Schmidt-Trucks{\"a}ss, Arno and Baumann, Marcus}, title = {Does increased blood pressure rather than aging influence retinal pulse wave velocity?}, series = {Investigative Ophthalmology \& Visual Science, IOVS}, volume = {53}, journal = {Investigative Ophthalmology \& Visual Science, IOVS}, number = {4}, publisher = {ARVO}, address = {Rockville, Md.}, issn = {0146-0404}, doi = {10.1167/iovs.11-8815}, pages = {2119 -- 2126}, year = {2012}, abstract = {Purpose: It was demonstrated previously that retinal pulse wave velocity (rPWV) as a measure of retinal arterial stiffness is increased in aged anamnestically healthy volunteers compared with young healthy subjects. Using novel methodology of rPWV assessment this finding was confirmed and investigated whether it might relate to the increased blood pressure usually accompanying the aging process, rather than to the aging itself. Methods: A total of 12 young 25.5-year-old (24.0-28.8) [median(1st quartile-3rd quartile)] and 12 senior 68.5-year-old (63.8-71.8) anamnestically healthy volunteers; and 12 senior 63.0-year-old (60.8-65.0) validated healthy volunteers and 12 young 33.0-year-old (29.5-35.0) hypertensive patients were examined. Time-dependent alterations of vessel diameter were assessed by the Dynamic Vessel Analyzer in a retinal artery of each subject. The data were filtered and processed using mathematical signal analysis and rPWVs were calculated. Results: rPWV amounted to 1200 (990-1470) RU (relative units)/s in the hypertensive group and to 1040 (700-2230) RU/s in anamnestically healthy seniors. These differed significantly from rPWVs in young healthy group (410 [280-500] RU/s) and in validated healthy seniors (400 [320-510] RU/s). rPWV associated with age and mean arterial pressure (MAP) in the pooled cohort excluded validated healthy seniors. In a regression model these associations remain when alternately adjusted for MAP and age. When including validated healthy seniors in the pooled cohort only association with MAP remains. Conclusions: Both aging (with not excluded cardiovascular risk factors) and mild hypertension are associated with elevated rPWV. rPWV increases to a similar extent both in young mildly hypertensive subjects and in aged anamnestically healthy persons. Healthy aging is not associated with increased rPWV.}, language = {en} } @article{KurulganDemirciDemirciLinderetal.2012, author = {Kurulgan Demirci, Eylem and Demirci, Taylan and Linder, Peter and Trzewik, J{\"u}rgen and Gierkowski, Jessica Ricarda and Gossmann, Matthias and Kayser, Peter and Porst, Dariusz and Digel, Ilya and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells}, series = {Journal of Bioscience and Bioengineering}, volume = {113}, journal = {Journal of Bioscience and Bioengineering}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1347-4421}, doi = {10.1016/j.jbiosc.2012.03.019}, pages = {212 -- 219}, year = {2012}, abstract = {All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.}, language = {en} } @article{HanssenNickelDrexeletal.2011, author = {Hanssen, H. and Nickel, T. and Drexel, V. and Hertel, G. and Emslander, I. and Sisic, Z. and Lorang, D. and Schuster, T. and Kotliar, Konstantin and Pressler, A. and Schmidt-Trucks{\"a}ss, A. and Weis, M. and Halle, M.}, title = {Exercise-induced alterations of retinal vessel diameters and cardiovascular risk reduction in obesity}, series = {Atherosclerosis}, volume = {216}, journal = {Atherosclerosis}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0021-9150}, pages = {433 -- 439}, year = {2011}, language = {en} } @article{KotliarKoshitzSvetlowaetal.2005, author = {Kotliar, Konstantin and Koshitz, I. N. and Svetlowa, O. V. and Makarov, F. N.}, title = {Biomechanical analysis of traditional and contemporary conceptions on pathogenesis of the primary open angle glaucoma / Koshitz, I. N. ; Svetlova, O. V. ; Kotliar, K. E. ; Makarov, F. N. ; Smolnikov, B. A.}, series = {Glaukoma (2005)}, journal = {Glaukoma (2005)}, publisher = {-}, pages = {41 -- 63}, year = {2005}, language = {en} } @article{KotliarSvetlovaStegaevetal.2004, author = {Kotliar, Konstantin and Svetlova, O. V. and Stegaev, V. A. and Parkhomov, S. D.}, title = {Biomechanical substantiation of relatively low efficiency of recurrent laser trabeculoplasty / Svetlova, O. V. ; Stagaev, V. A. ; Parkhomov, S. D. ; Kotliar, K. E. ; Makarov, F. N. ; Smolnikov, B. A. ; Koshitz, I. N.}, series = {Glaukoma (2004)}, journal = {Glaukoma (2004)}, publisher = {-}, pages = {29 -- 39}, year = {2004}, language = {en} } @article{KotliarSvetlovaSourjikovetal.2004, author = {Kotliar, Konstantin and Svetlova, O. V. and Sourjikov, A. V. and Zaseeva, M. V.}, title = {Biomechanical peculiarities of aqueous humor production system and outflow regulation system / Svetlova, O. V. ; Sourjikov, A. V. ; Kotliar, K. E. ; Zaseeva, M. V. ; Shukhaev, S. V. ; Koshitz, I. N.}, series = {Glaukoma (2004)}, journal = {Glaukoma (2004)}, publisher = {-}, pages = {66 -- 76}, year = {2004}, language = {en} } @article{Digel2011, author = {Digel, Ilya}, title = {Primary thermosensory events in cells}, series = {Transient receptor potential channels / Md. Shahidul Islam, ed.}, journal = {Transient receptor potential channels / Md. Shahidul Islam, ed.}, publisher = {Springer}, address = {Dordrecht [u.a.]}, isbn = {978-94-007-0264-6}, pages = {451 -- 468}, year = {2011}, language = {en} }