@article{BaeckerKochEibenetal.2017, author = {B{\"a}cker, Matthias and Koch, Claudia and Eiben, Sabine and Geiger, Fania and Eber, Fabian and Gliemann, Hartmut and Poghossian, Arshak and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors}, series = {Sensors and Actuators B: Chemical}, volume = {238}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.07.096}, pages = {716 -- 722}, year = {2017}, abstract = {The conjunction of (bio-)chemical recognition elements with nanoscale biological building blocks such as virus particles is considered as a very promising strategy for the creation of biohybrids opening novel opportunities for label-free biosensing. This work presents a new approach for the development of biosensors using tobacco mosaic virus (TMV) nanotubes or coat proteins (CPs) as enzyme nanocarriers. Sensor chips combining an array of Pt electrodes loaded with glucose oxidase (GOD)-modified TMV nanotubes or CP aggregates were used for amperometric detection of glucose as a model system for the first time. The presence of TMV nanotubes or CPs on the sensor surface allows binding of a high amount of precisely positioned enzymes without substantial loss of their activity, and may also ensure accessibility of their active centers for analyte molecules. Specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CPs was achieved via bioaffinity binding. These layouts were tested in parallel with glucose sensors with adsorptively immobilized [SA]-GOD, as well as [SA]-GOD crosslinked with glutardialdehyde, and came out to exhibit superior sensor performance. The achieved results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for future applications in biosensorics and biochips.}, language = {en} } @incollection{KochPoghossianWegeetal.2018, author = {Koch, Claudia and Poghossian, Arshak and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {TMV-Based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications}, series = {Virus-Derived Nanoparticles for Advanced Technologies}, booktitle = {Virus-Derived Nanoparticles for Advanced Technologies}, editor = {Wege, Christina}, publisher = {Humana Press}, address = {New York, NY}, isbn = {978-1-4939-7808-3}, doi = {10.1007/978-1-4939-7808-3}, pages = {553 -- 568}, year = {2018}, abstract = {Nanotubular tobacco mosaic virus (TMV) particles and RNA-free lower-order coat protein (CP) aggregates have been employed as enzyme carriers in different diagnostic layouts and compared for their influence on biosensor performance. In the following, we describe a label-free electrochemical biosensor for improved glucose detection by use of TMV adapters and the enzyme glucose oxidase (GOD). A specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CP aggregates was achieved via bioaffinity binding. Glucose sensors with adsorptively immobilized [SA]-GOD, and with [SA]-GOD cross-linked with glutardialdehyde, respectively, were tested in parallel on the same sensor chip. Comparison of these sensors revealed that TMV adapters enhanced the amperometric glucose detection remarkably, conveying highest sensitivity, an extended linear detection range and fastest response times. These results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for applications in biosensorics and biochips. Here, we describe the fabrication and use of amperometric sensor chips combining an array of circular Pt electrodes, their loading with GOD-modified TMV nanotubes (and other GOD immobilization methods), and the subsequent investigations of the sensor performance.}, language = {en} } @article{KochPoghossianSchoeningetal.2018, author = {Koch, Claudia and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Wege, Christian}, title = {Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors}, series = {Nanotheranostics}, volume = {2}, journal = {Nanotheranostics}, number = {2}, publisher = {Ivyspring}, address = {Sydney}, issn = {2206-7418}, doi = {10.7150/ntno.22114}, pages = {184 -- 196}, year = {2018}, abstract = {The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.}, language = {en} } @book{Grotendorst2012, author = {Grotendorst, Johannes}, title = {Hierarchical methods for dynamics in complex molecular systems}, series = {Schriften des Forschungszentrums J{\"u}lich IAS Series 10}, journal = {Schriften des Forschungszentrums J{\"u}lich IAS Series 10}, publisher = {Forschungszentrum J{\"u}lich}, address = {J{\"u}lich}, isbn = {978-3-89336-768-9}, pages = {VI, 540 S. zahlr. Ill. u. graph. Darst.}, year = {2012}, language = {en} } @inproceedings{JablonskiKochBronderetal.2017, author = {Jablonski, Melanie and Koch, Claudia and Bronder, Thomas and Poghossian, Arshak and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Field-Effect Biosensors Modified with Tobacco Mosaic Virus Nanotubes as Enzyme Nanocarrier}, series = {MDPI Proceeding}, volume = {1}, booktitle = {MDPI Proceeding}, number = {4}, doi = {10.3390/proceedings1040505}, pages = {4}, year = {2017}, language = {en} } @article{BegingMlynekHataihimakuletal.2010, author = {Beging, Stefan and Mlynek, Daniela and Hataihimakul, Sudkanung and Poghossian, Arshak and Baldsiefen, Gerhard and Busch, Heinz and Laube, Norbert and Kleinen, Lisa and Sch{\"o}ning, Michael Josef}, title = {Field-effect calcium sensor for the determination of the risk of urinary stone formation}, series = {Sensors and Actuators B: Chemical}, volume = {144}, journal = {Sensors and Actuators B: Chemical}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2008.12.012}, pages = {374 -- 379}, year = {2010}, abstract = {Urinary stone formation has been evolved to a widespread disease during the last years. The reason for the formation of urinary stones are little crystals, mostly composed of calcium oxalate, which are formed in human kidneys. The early diagnosis of the risk for urinary stone formation of patients can be determined by the "Bonn-Risk-Index" method based on the potentiometric detection of the Ca2+-ion concentration and an optical determination of the triggered crystallisation of calcium oxalate in unprocessed urine. In this work, miniaturised capacitive field-effect EMIS (electrolyte-membrane-insulator-semiconductor) sensors have been developed for the determination of the Ca2+-ion concentration in human native urine. The Ca2+-sensitive EMIS sensors have been systematically characterised by impedance spectroscopy, capacitance-voltage and constant-capacitance method in terms of sensitivity, signal stability and response time in both CaCl2 solutions and in native urine. The obtained results demonstrate the suitability of EMIS sensors for the measurement of the Ca2+-ion concentration in native urine of patients.}, language = {en} } @article{ReisertHenkelSchneideretal.2010, author = {Reisert, Steffen and Henkel, Hartmut and Schneider, Andreas and Sch{\"a}fer, Daniel and Friedrich, Peter and Berger, J{\"o}rg and Sch{\"o}ning, Michael Josef}, title = {Development of a handheld sensor system for the online measurement of hydrogen peroxide in aseptic filling systems}, series = {Physica Status Solidi (A)}, volume = {207}, journal = {Physica Status Solidi (A)}, number = {4}, publisher = {Wiley-VCH}, address = {Berlin}, issn = {1862-6300}, doi = {10.1002/pssa.200983304}, pages = {913 -- 918}, year = {2010}, abstract = {A handheld sensor system for the online measurement of hydrogen peroxide (H2O2) in aseptic sterilisation processes has been developed. It is based on a calorimetric-type gas sensor that consists of a differential set-up of two temperature sensors, of which one is catalytically activated and the second one is passivated and used as reference. The sensor principle relies in detecting a rise in temperature on the active sensor due to the exothermic reaction of H2O2 on the catalytic surface. To characterise the sensor system towards H2O2 sensitivity and other influencing factors, measurements have been carried out both at an experimental set-up and a manufacturer's sterilisation machine. Physical sensor characterisation was done by means of the optical microscopy.}, language = {en} } @article{MiyamotoSugawaraKanohetal.2010, author = {Miyamoto, Ko-ichiro and Sugawara, Yuri and Kanoh, Shin´ichiro and Yoshinobu, Tatsuo and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Image correction method for the chemical imaging sensor}, series = {Sensors and Actuators B: Chemical}, volume = {144}, journal = {Sensors and Actuators B: Chemical}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2008.10.069}, pages = {344 -- 348}, year = {2010}, abstract = {The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the spatial distribution of chemical species. For the practical application of this sensor, artifacts in the chemical images due to defects of the semiconductor substrate and contamination of the sensing surface etc. have been a major problem. An image correction method was developed to eliminate the influence of nonuniformity of individual sensor plate.}, language = {en} } @article{AbouzarPoghossianSiqueiraetal.2010, author = {Abouzar, Maryam H. and Poghossian, Arshak and Siqueira, Jos{\´e} R. Jr. and Oliveira, Osvaldo N. Jr. and Moritz, Werner and Sch{\"o}ning, Michael Josef}, title = {Capacitive electrolyte-insulator-semiconductor structures functionalised with a polyelectrolyte/enzyme multilayer: New strategy for enhanced field-effect biosensing}, series = {Physica Status Solidi (A)}, volume = {207}, journal = {Physica Status Solidi (A)}, number = {4}, publisher = {Wiley-VCH}, address = {Berlin}, issn = {1862-6300}, doi = {10.1002/pssa.200983317}, pages = {884 -- 890}, year = {2010}, abstract = {A novel strategy for enhanced field-effect biosensing using capacitive electrolyte-insulator-semiconductor (EIS) structures functionalised with pH-responsive weak polyelectrolyte/enzyme or dendrimer/enzyme multilayers is presented. The feasibility of the proposed approach is exemplarily demonstrated by realising a penicillin biosensor based on a capacitive p-Si-SiO2 EIS structure functionalised with a poly(allylamine hydrochloride) (PAH)/penicillinase and a poly(amidoamine) dendrimer/penicillinase multilayer. The developed sensors response to changes in both the local pH value near the gate surface and the charge of macromolecules induced via enzymatic reaction, resulting in a higher sensitivity. For comparison, an EIS penicillin biosensor with adsorptively immobilised penicillinase has been also studied. The highest penicillin sensitivity of 100 mV/dec has been observed for the EIS sensor functionalised with the PAH/penicillinase multilayer. The lower and upper detection limit was around 20 µM and 10 mM, respectively. In addition, an incorporation of enzymes in a multilayer prepared by layer-by-layer technique provides a larger amount of immobilised enzymes per sensor area, reduces enzyme leaching effects and thus, enhances the biosensor lifetime (the loss of penicillin sensitivity after 2 months was 10-12\%).}, language = {en} } @article{SchoeningTsarouchasBeckersetal.1996, author = {Sch{\"o}ning, Michael Josef and Tsarouchas, D. and Beckers, Leah and Schubert, J. and Zander, W. and Kordoš, P. and L{\"u}th, Hans}, title = {A highly long-term stable silicon-based pH sensor using pulsed laser deposition technique}, series = {Sensors and Actuators B. 35 (1996), H. 1-3}, journal = {Sensors and Actuators B. 35 (1996), H. 1-3}, isbn = {0925-4005}, pages = {228 -- 233}, year = {1996}, language = {en} }