@techreport{SiegertBongaertsWagneretal.2022,
  author    = {Siegert, Petra and Bongaerts, Johannes and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Selmer, Thorsten},
  title     = {Abschlussbericht zum Projekt zur {\"U}berwachung biotechnologischer Prozesse mittels Diacetyl-/Acetoin-Biosensor und Evaluierung von Acetoin-Reduktasen zur Verwendung in Biotransformationen},
  address   = {Aachen},
  organization    = {FH Aachen},
  pages     = {16 Seiten},
  year      = {2022},
  language  = {de}
}
@article{TrappLammersEngudaretal.2023,
  author    = {Trapp, Svenja and Lammers, Tom and Engudar, Gokce and Hoehr, Cornelia and Denkova, Antonia G. and Paulßen, Elisabeth and de Kruijff, Robin M.},
  title     = {Membrane-based microfluidic solvent extraction of Ga-68 from aqueous Zn solutions: towards an automated cyclotron production loop},
  series = {EJNMMI Radiopharmacy and Chemistry},
  volume    = {2023},
  journal   = {EJNMMI Radiopharmacy and Chemistry},
  number    = {8, Article number: 9},
  publisher = {Springer Nature},
  issn      = {2365-421X},
  doi       = {10.1186/s41181-023-00195-2},
  pages     = {1 -- 14},
  year      = {2023},
  language  = {en}
}
@unpublished{GreinerJerominSitholeetal.2023,
  author    = {Greiner, Lasse and Jeromin, G{\"u}nter Erich and Sithole, Patience and Petersen, Soenke},
  title     = {Preprint: Studies on the enzymatic reduction of levulinic acid using Chiralidon-R and Chiralidon-S},
  series = {ChemRxiv},
  journal   = {ChemRxiv},
  doi       = {10.26434/chemrxiv-2023-jlvcv},
  pages     = {13 Seiten},
  year      = {2023},
  abstract  = {The enzymatic reduction of levulinic acid by the chiral catalysts Chiralidon-R and Chiralidon-S which are commercially available superabsorbed alcohol dehydrogenases is described. The Chiralidon®-R/S reduces the levulinic acid to the (R,S)-4-hydroxy valeric acid and the (R)- or (S)- gamma-valerolactone.},
  language  = {en}
}
@article{ElBerguiAbouabdillahBouriougetal.2023,
  author    = {El Bergui, Omnia and Abouabdillah, Aziz and Bourioug, Mohamed and Schmitz, Dominik and Biel, Markus and Aboudrare, Abdellah and Krauss, Manuel and Jomaa, Ahlem and Romuli, Sebastian and M{\"u}ller, Joachim and Fagroud, Mustapha and Bouabid, Rachid},
  title     = {Innovative solutions for drought: Evaluating hydrogel application on onion cultivation (Allium cepa) in Morocco},
  series = {Water},
  volume    = {15},
  journal   = {Water},
  number    = {11},
  publisher = {MDPI},
  address   = {Basel},
  doi       = {10.3390/w15111972},
  pages     = {Artikel 1972},
  year      = {2023},
  abstract  = {Throughout the last decade, and particularly in 2022, water scarcity has become a critical concern in Morocco and other Mediterranean countries. The lack of rainfall during spring was worsened by a succession of heat waves during the summer. To address this drought, innovative solutions, including the use of new technologies such as hydrogels, will be essential to transform agriculture. This paper presents the findings of a study that evaluated the impact of hydrogel application on onion (Allium cepa) cultivation in Meknes, Morocco. The treatments investigated in this study comprised two different types of hydrogel-based soil additives (Arbovit® polyacrylate and Huminsorb® polyacrylate), applied at two rates (30 and 20 kg/ha), and irrigated at two levels of water supply (100\% and 50\% of daily crop evapotranspiration; ETc). Two control treatments were included, without hydrogel application and with both water amounts. The experiment was conducted in an open field using a completely randomized design. The results indicated a significant impact of both hydrogel-type dose and water dose on onion plant growth, as evidenced by various vegetation parameters. Among the hydrogels tested, Huminsorb® Polyacrylate produced the most favorable outcomes, with treatment T9 (100\%, HP, 30 kg/ha) yielding 70.55 t/ha; this represented an increase of 11 t/ha as compared to the 100\% ETc treatment without hydrogel application. Moreover, the combination of hydrogel application with 50\% ETc water stress showed promising results, with treatment T4 (HP, 30 kg, 50\%) producing almost the same yield as the 100\% ETc treatment without hydrogel while saving 208 mm of water.},
  language  = {en}
}
@misc{BraunKrafftTippkoetter2022,
  author    = {Braun, Lena and Krafft, Simone and Tippk{\"o}tter, Nils},
  title     = {Combined supercritical carbon dioxide extraction and chromatography of the algae fatty linoleic and linolenic acid},
  series = {Chemie Ingenieur Technik},
  volume    = {94},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.202255308},
  pages     = {1304},
  year      = {2022},
  abstract  = {A method for the integrated extraction and separation of fatty acids from algae using supercritical CO2 is presented. Desmodesmus obliquus and Chlorella sorokiniana were used as algae. First, a method for chromatographic separation of fatty acids of different degrees of saturation was established and optimized. Then, an integrated method for supercritical extraction was developed for both algal species. It was also verified whether prior cell disruption was beneficial for extraction. In developing the method for chromatographic separation, statistical experimental design was used to determine the optimal parameter settings. The methanol content in the mobile phase proved to be the most important parameter for successful separation of the three unsaturated fatty acids oleic acid, linoleic acid, and linolenic acid. Supercritical extraction with dried algae showed that about four times more fatty acids can be extracted from C. sorokiniana relative to the dry mass used.},
  language  = {en}
}
@misc{KrafftKukaUlberetal.2022,
  author    = {Krafft, Simone and Kuka, Katrin and Ulber, Roland and Tippk{\"o}tter, Nils},
  title     = {Utilization of Lolium perenne varieties as a renewable substrate for single-cell proteins, lactate, and composite materials},
  series = {Chemie Ingenieur Technik},
  volume    = {94},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.202255306},
  pages     = {1303 -- 1304},
  year      = {2022},
  abstract  = {Lolium perenne (perennial ryegrass) is aproductive and high-quality forage grass indigenous to Southern Europe, temperate Asia, and North Africa. Nowadays it is widespread and the dominant grass species on green areas in temperate climates. This abundant source of biomass is suitable for the development of bioeconomic processes because of its high cellulose and water-soluble carbohydrate content. In this work, novel breeds of the perennial ryegrass are being examined with regards to their quality parameters and biotechnological utilization options within the context of bioeconomy. Three processing operations are presented. In the first process, the perennial ryegrass is pretreated by pressing or hydrothermal extraction to derive glucosevia subsequent enzymatic hydrolysis of cellulose. A yield of up to 82 \% glucose was achieved when using the hydrothermal ex-traction as pretreatment. In a second process, the ryegrass is used to produce lactic acid in high concentrations. The influence of the growth conditions and the cutting time on the carboxylic acid yield is investigated. A yield of lactic acid of above 150 g kg⁻¹ dry matter was achieved. The third process is to use Lolium perenne as a substrate in the fermentation of K. marxianus for the microbial production of single-cell proteins. The perennial ryegrass is screw-pressed and the press juice is used as medium. When supplementing the press juice with yeast media components, a biomass concentration of up to 16 g L⁻¹ could be achieved.},
  language  = {en}
}
@misc{VarrialeKukaTippkoetteretal.2022,
  author    = {Varriale, Ludovica and Kuka, Katrin and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Use of a green biomass in a biorefinery platform},
  series = {Chemie Ingenieur Technik},
  volume    = {94},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.202255095},
  pages     = {1299},
  year      = {2022},
  abstract  = {The emerging environmental issues due to the use of fossil resources are encouraging the exploration of new renewable resources. Biomasses are attracting more interest due to the low environmental impacts, low costs, and high availability on earth. In this scenario, green biorefineries are a promising platform in which green biomasses are used as feedstock. Grasses are mainly composed of cellulose and hemicellulose, and lignin is available in a small amount. In this work, a perennial ryegrass was used as feedstock to develop a green bio-refinery platform. Firstly, the grass was mechanically pretreated, thus obtaining a press juice and a press cake fraction. The press juice has high nutritional values and can be employed as part of fermentation media. The press cake can be employed as a substrate either in enzymatic hydrolysis or in solid-state fermentation. The overall aim of this work was to demonstrate different applications of both the liquid and the solid fractions. For this purpose, the filamentous fungus A. niger and the yeast Y. lipolythica were selected for their ability to produce citric acid. Finally, the possibility was assessed to use the press juice as part of fermentation media to cultivate S. cerevisiae and lactic acid bacteria for ethanol and lactic acid fermentation.},
  language  = {en}
}
@misc{RothkranzKrafftTippkoetter2022,
  author    = {Rothkranz, Berit and Krafft, Simone and Tippk{\"o}tter, Nils},
  title     = {Media optimization for sustainable fuel production: How to produce biohydrogen from renewable resources with Thermotoga neapolitana},
  series = {Chemie Ingenieur Technik},
  volume    = {94},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.202255305},
  pages     = {1298 -- 1299},
  year      = {2022},
  abstract  = {Hydrogen is playing an increasingly important role in research and politics as an energy carrier of the future. Since hydrogen has commonly been produced from methane by steam reforming, the need for climate-friendly, alternative production routes is emerging. In addition to electrolysis, fermentative routes for the production of so-called biohydrogen are "green" alternatives. The application of microorganisms offers the advantage of sustainable production from renewable resources using easily manageable technologies. In this project, the hyperthermophilic, anaerobic microorganism Thermotoga neapolitana is used for the productio nof biohydrogen from renewable resources. The enzymatically hydrolyzed resources were used in fermentation leading to yield coefficients of 1.8 mole H₂ per mole glucose when using hydrolyzed straw and ryegrass supplemented with medium, respectively. These results are similar to the hydrogen yields when using Thermotoga basal medium with glucose (TBGY) as control group. In order to minimize the supplementation of the hydrolysate and thus increase the economic efficiency of the process, the essential media components were identified. The experiments revealed NaCl, KCl, and glucose as essential components for cell growth as well as biohydrogen production. When excluding NaCl, a decrease of 96\% in hydrogen production occured.},
  language  = {en}
}
@techreport{Tippkoetter2021,
  author    = {Tippk{\"o}tter, Nils},
  title     = {TreBec - Herstellung eines Mehrwegbechers aus Treber : Sachbericht zum Verwendungsnachweis},
  publisher = {FH Aachen},
  address   = {J{\"u}lich},
  doi       = {10.2314/KXP:1858723302},
  pages     = {10 Seiten},
  year      = {2021},
  abstract  = {Laufzeit des Vorhabens und Berichtszeitraum: 01.10.2020-30.09.2021},
  language  = {de}
}
@misc{SiekerTippkoetterUlberetal.2010,
  author    = {Sieker, T. and Tippk{\"o}tter, Nils and Ulber, Roland and Bart, H.-J. and Heinzle, E.},
  title     = {Gr{\"u}ne Bioraffinerie: Ganzheitliche Nutzung von Grassilage f{\"u}r die Herstellung von Grund-und Feinchemikalien},
  series = {Chemie Ingenieur Technik},
  volume    = {82},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.201050321},
  pages     = {1564},
  year      = {2010},
  abstract  = {Gras hat ein hohes Potenzial als nachwachsender Rohstoff. Bei ungeeigneter Lagerung verderben Gr{\"a}ser allerdings innerhalb weniger Tage. Dieser Nachteil kann durch die Silierung des Grasschnittes behoben werden. Eines der wichtigsten in der Silage enthaltenen Produkte ist die Milchs{\"a}ure. Um diese f{\"u}r weitere Aufarbeitungsschritte zug{\"a}nglich zu machen, wird aus der Silage ein Presssaft hergestellt. Die Milchs{\"a}ure wird aus einem Silagepresssaft mittels Extraktion durch ionische Fl{\"u}ssigkeiten isoliert. Dabei wird zum einen eine reine Milchs{\"a}ure hergestellt, die z. B. f{\"u}r die Herstellung von Polymilchs{\"a}ure genutzt werden kann. Zum anderen wird ein weniger aufgereinigter Extrakt gewonnen, der f{\"u}r die fermentative Produktion von L-Lysin und 1,2-Propandiol genutzt werden soll. Im Rahmen des Projekts erfolgt die gentechnische Optimierung von Corynebacterium glutamicum f{\"u}r die Umsetzung von Milchs{\"a}urezu L-Lysin. Die im nach der Pressung verbleibenden Presskuchen enthaltenen Grasfasern bestehen zu einem großen Teil aus Polysacchariden. Diese werden hydrolysiert und die dabei freigesetzten Zucker zu Grundchemikalien wie Ethanol oder Itakons{\"a}ure fermentiert. Im Rahmen einer vollst{\"a}ndigen Nutzung der Silage wird das Raffinat aus der Milchs{\"a}ureextraktion als Mediumsupplement in der Fermentation eingesetzt, was die Zugabe weiterer Medienbestandteile {\"u}berfl{\"u}ssig macht. Die R{\"u}ckst{\"a}nde der Hydrolysen und Fermentationen sollen dar{\"u}berhinaus f{\"u}r die Herstellung von Biogas genutzt werden.},
  language  = {de}
}