@inproceedings{Goldbach2010,
  author    = {Goldbach, Daniel},
  title     = {Management der funktionalen Sicherheit einer Entwicklung eines sicherheitskritischen Systems im Bereich Nutzfahrzeuge},
  series = {Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)},
  booktitle = {Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)},
  publisher = {Shaker},
  address   = {Aachen},
  isbn      = {978-3-8322-9040-5},
  pages     = {487 -- 495},
  year      = {2010},
  language  = {de}
}
@inproceedings{GoldbachAment2010,
  author    = {Goldbach, Daniel and Ament, Christoph},
  title     = {Stabilized reversing with vehicle trailer combinations},
  series = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau},
  booktitle = {Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau},
  editor    = {Scharff, Peter},
  publisher = {Verl. ISLE},
  address   = {Ilmenau},
  organization    = {Technische Universit{\"a}t <Ilmenau> / Fakult{\"a}t f{\"u}r Informatik und Automatisierung},
  isbn      = {978-3-938843-53-6},
  pages     = {352 -- 357},
  year      = {2010},
  language  = {en}
}
@inproceedings{BungSchlenkhoff2010,
  author    = {Bung, Daniel Bernhard and Schlenkhoff, Andreas},
  title     = {Selbstbel{\"u}ftete Gerinnestr{\"o}mungen auf Treppenschussrinnen},
  series = {Talsperren im Wandel : 15. Deutsches Talsperrensymposium ; Beitr{\"a}ge zur Tagung am 14. bis 16. April 2010 im Eurogress Aachen},
  booktitle = {Talsperren im Wandel : 15. Deutsches Talsperrensymposium ; Beitr{\"a}ge zur Tagung am 14. bis 16. April 2010 im Eurogress Aachen},
  organization    = {Deutsches TalsperrenKomitee},
  isbn      = {978-3-8322-9043-6},
  pages     = {39 -- 47},
  year      = {2010},
  language  = {de}
}
@inproceedings{KerpenBungSchlurmann2010,
  author    = {Kerpen, N. B. and Bung, Daniel Bernhard and Schlurmann, Torsten},
  title     = {Physical model investigations of ships passing through a lock},
  series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010},
  booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010},
  editor    = {Janssen, Robert},
  publisher = {School of Civil Engineeering, The University of Queensland},
  address   = {Brisbane},
  organization    = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>},
  isbn      = {9781742720159},
  pages     = {93 -- 100},
  year      = {2010},
  language  = {en}
}
@inproceedings{KerpenBungSchlurmann2010,
  author    = {Kerpen, N. B. and Bung, Daniel Bernhard and Schlurmann, Torsten},
  title     = {Physical model investigations of pressure distributions next to ships passing through a lock},
  series = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010},
  booktitle = {5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010},
  publisher = {Univ. Press},
  address   = {Tianjin},
  organization    = {Chinese-German Joint Symposium on Hydraulic and Ocean Engineering <5, 2010, Tianjin>},
  isbn      = {978-7-5618-3671-2},
  pages     = {514 -- 519},
  year      = {2010},
  language  = {en}
}
@misc{Alt2010,
  author    = {Alt, Helmut},
  title     = {Vom Studium der Kerntechnik zu Energie zwischen Wunsch und Wirklichkeit : [Vortrag : Verleihung der KTG-Ehrenmitgliedschaft, Berlin, November 2010]},
  pages     = {1 -- 15},
  year      = {2010},
  language  = {de}
}
@article{RibitschKarlBirnerGruenbergeretal.2010,
  author    = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.},
  title     = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli},
  series = {Journal of biotechnology},
  volume    = {150},
  journal   = {Journal of biotechnology},
  number    = {3},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2010.09.947},
  pages     = {408 -- 416},
  year      = {2010},
  abstract  = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.},
  language  = {en}
}
@misc{SiegertMussmannO'Connelletal.2010,
  author    = {Siegert, Petra and Mussmann, Nina and O'Connell, Timothy and Maurer, Karl-Heinz},
  title     = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 30},
  year      = {2010},
  language  = {de}
}
@misc{SiegertBaumstarkKluinetal.2010,
  author    = {Siegert, Petra and Baumstark, Rebecca and Kluin, Cornelia and O'Connell, Timothy and Maurer, Karl-Heinz and Hellmuth, Hendrik},
  title     = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt},
  address   = {M{\"u}nchen},
  pages     = {1 -- 30},
  year      = {2010},
  language  = {de}
}
@misc{SiegertSpitzMaurer2010,
  author    = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz},
  title     = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patentamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 31},
  year      = {2010},
  language  = {de}
}