@incollection{OezdilBerndtHoecker1991, author = {{\"O}zdil, S. and Berndt, Heinz and H{\"o}cker, Hartwig}, title = {Systematische Erfassung der Best{\"a}ndigkeit von Ryton-, P84- und Dralon ATF 1063-Fasern unter Einwirkung verschiedener NOx-Konzentrationen in einem definierten Temperaturintervall}, series = {Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V.}, booktitle = {Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V.}, number = {107}, publisher = {Dt. Wollforschungsinstitut}, address = {Aachen}, issn = {0930-3723}, pages = {89 -- 129}, year = {1991}, language = {de} } @article{OehlschlaegerSteinbergSehretal.2005, author = {{\"O}hlschl{\"a}ger, Peter and Steinberg, Thorsten and Sehr, Peter and Osen, Wolfram}, title = {Modification of HPV 16 E7 genes: correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice / Steinberg, Thorsten ; {\"O}hlschl{\"a}ger, Peter ; Sehr, Peter ; Osen, Wolfram ; Gissmann, Lutz}, series = {Vaccine. 23 (2005), H. 9}, journal = {Vaccine. 23 (2005), H. 9}, isbn = {0264-410X}, pages = {1149 -- 1157}, year = {2005}, language = {en} } @article{OehlschlaegerSpiesAlvarezetal.2011, author = {{\"O}hlschl{\"a}ger, Peter and Spies, Elmar and Alvarez, Gerardo and Quetting, Michael and Groettrup, Marcus}, title = {The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA}, series = {International Journal of Cancer. 128 (2011), H. 2}, journal = {International Journal of Cancer. 128 (2011), H. 2}, publisher = {Wiley}, address = {Weinheim}, isbn = {1097-0215}, pages = {473 -- 481}, year = {2011}, language = {en} } @article{OehlschlaegerQuettingAlvarezetal.2009, author = {{\"O}hlschl{\"a}ger, Peter and Quetting, Michael and Alvarez, Gerardo and D{\"u}rst, Matthias and Gissmann, Lutz and Kaufmann, Andreas M.}, title = {Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants}, series = {International Journal of Cancer}, volume = {125}, journal = {International Journal of Cancer}, number = {1}, publisher = {Wiley}, address = {Weinheim}, isbn = {1097-0215}, pages = {189 -- 198}, year = {2009}, language = {en} } @article{OehlschlaegerPesOsenetal.2006, author = {{\"O}hlschl{\"a}ger, Peter and Pes, Michaela and Osen, Wolfram and D{\"u}rst, Matthias}, title = {An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response / {\"O}hlschl{\"a}ger, Peter ; Pes, Michaela ; Osen, Wolfram ; D{\"u}rst, Matthias ; Schneider, Achim ; Gissmann, Lutz ; Kaufman}, series = {Vaccine. 24 (2006), H. 15}, journal = {Vaccine. 24 (2006), H. 15}, isbn = {0264-410X}, pages = {2880 -- 2893}, year = {2006}, language = {en} } @article{OehlschlaegerOsenPeileretal.2001, author = {{\"O}hlschl{\"a}ger, Peter and Osen, Wolfram and Peiler, Tanja and Caldeira, Sandra}, title = {A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity / Osen, Wolfram ; Peiler, Tanja ; {\"O}hlschl{\"a}ger, Peter ; Caldeira, Sandra ; Faath, Stefan ; Mich}, series = {Vaccine. 19 (2001), H. 20}, journal = {Vaccine. 19 (2001), H. 20}, isbn = {0264-410X}, pages = {4276 -- 4286}, year = {2001}, language = {en} } @article{OehlschlaegerOsenDelletal.2003, author = {{\"O}hlschl{\"a}ger, Peter and Osen, Wolfram and Dell, Kerstin and Faath, Stefan}, title = {Human papillomavirus type 16 L1 capsomeres induce L1-specific cytotoxic T lymphocytes and tumor regression in C57BL/6 mice / {\"O}hlschl{\"a}ger, Peter ; Osen, Wolfram ; Dell, Kerstin ; Faath, Stefan ; Garcea Robert L: ; Jochmus, Ingrid ; M{\"u}ller, Martin, Pawlita,}, series = {Journal of Virology. 77 (2003), H. 8}, journal = {Journal of Virology. 77 (2003), H. 8}, isbn = {1098-5514}, pages = {4635 -- 4645}, year = {2003}, language = {en} } @article{OehlschlaegerMichelOsenetal.2002, author = {{\"O}hlschl{\"a}ger, Peter and Michel, Nico and Osen, Wolfram and Freyschmidt, Eva-Jasmin}, title = {T cell response to human papillomavirus 16 E7 in mice: comparison of Cr release assay, intracellular IFN-gamma production, ELISPOT and tetramer staining / Michel, Nico ; {\"O}hlschl{\"a}ger, Peter ; Osen, Wolfram ; Freyschmidt, Eva-Jasmin ; Gut{\"o}hrlein, Heidrun ;}, series = {Intervirology. 45 (2002)}, journal = {Intervirology. 45 (2002)}, isbn = {1423-0100}, pages = {290 -- 299}, year = {2002}, language = {en} } @article{OehlschlaegerCorvinusOrthetal.2005, author = {{\"O}hlschl{\"a}ger, Peter and Corvinus, Florian M. and Orth, Carina and Moriggl, Richard}, title = {Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth / Corvinus, Florian, Moriggl, Richard ; Tsareva, Svetlana A. ; Wagner, Stefan ; Pfitzner, Edith B. ; Baus, Daniela ; Kaufmann, Roland : Huber, Luka}, series = {Neoplasia. 7 (2005), H. 6}, journal = {Neoplasia. 7 (2005), H. 6}, isbn = {1476-5586}, pages = {545 -- 555}, year = {2005}, language = {en} } @article{ZientzBongaertsUnden1998, author = {Zientz, Evelyn and Bongaerts, Johannes and Unden, Gottfried}, title = {Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system}, series = {Journal of bacteriology}, volume = {Vol. 180}, journal = {Journal of bacteriology}, number = {No. 20}, issn = {1098-5530 (E-Journal); 0021-9193 (Print)}, pages = {5421 -- 5425}, year = {1998}, language = {en} } @article{ZhantlessovaSavitskayaKistaubayevaetal.2022, author = {Zhantlessova, Sirina and Savitskaya, Irina and Kistaubayeva, Aida and Ignatova, Ludmila and Talipova, Aizhan and Pogrebnjak, Alexander and Digel, Ilya}, title = {Advanced "Green" prebiotic composite of bacterial cellulose/pullulan based on synthetic biology-powered microbial coculture strategy}, series = {Polymers}, volume = {14}, journal = {Polymers}, number = {15}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym14153224}, pages = {Artikel 3224}, year = {2022}, abstract = {Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.}, language = {en} } @article{ZhangHeimbachScheeretal.2016, author = {Zhang, Jin and Heimbach, Tycho and Scheer, Nico and Barve, Avantika and Li, Wenkui and Lin, Wen and He, Handan}, title = {Clinical Exposure Boost Predictions by Integrating Cytochrome P450 3A4-Humanized Mouse Studies With PBPK Modeling}, series = {Journal of Pharmaceutical Sciences}, volume = {Volume 105}, journal = {Journal of Pharmaceutical Sciences}, number = {Issue 4}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0022-3549}, doi = {doi.org/10.1016/j.xphs.2016.01.021}, pages = {1398 -- 1404}, year = {2016}, abstract = {NVS123 is a poorly water-soluble protease 56 inhibitor in clinical development. Data from in vitro hepatocyte studies suggested that NVS123 is mainly metabolized by CYP3A4. As a consequence of limited solubility, NVS123 therapeutic plasma exposures could not be achieved even with high doses and optimized formulations. One approach to overcome NVS123 developability issues was to increase plasma exposure by coadministrating it with an inhibitor of CYP3A4 such as ritonavir. A clinical boost effect was predicted by using physiologically based pharmacokinetic (PBPK) modeling. However, initial boost predictions lacked sufficient confidence because a key parameter, fraction of drug metabolized by CYP3A4 (ƒₘCYP3A4), could not be estimated with accuracy on account of disconnects between in vitro and in vivo preclinical data. To accurately estimate ƒₘCYP3A4 in human, an in vivo boost effect study was conducted using CYP3A4-humanized mouse model which showed a 33- to 56-fold exposure boost effect. Using a top-down approach, human ƒₘCYP3A4 for NVS123 was estimated to be very high and included in the human PBPK modeling to support subsequent clinical study design. The combined use of the in vivo boost study in CYP3A4-humanized mouse model mice along with PBPK modeling accurately predicted the clinical outcome and identified a significant NVS123 exposure boost (∼42-fold increase) with ritonavir.}, language = {en} } @inproceedings{ZahnBerndtFehrenbach1973, author = {Zahn, Helmut and Berndt, Heinz and Fehrenbach, P.}, title = {Synthese cyclischer Cystinpeptide mit Insulin A-und B-Kettensequenzen}, series = {Peptides 1972 : proceedings of the Twelfth European Peptide Symposium, Reinhardsbrunn Castle, German Democratic Republic, September 1972}, booktitle = {Peptides 1972 : proceedings of the Twelfth European Peptide Symposium, Reinhardsbrunn Castle, German Democratic Republic, September 1972}, editor = {Hanson, Horst}, publisher = {North-Holland Publ. [u.a.],}, address = {Amsterdam [u.a.]}, isbn = {0-7204-4132-3}, pages = {101 -- 102}, year = {1973}, language = {de} } @inproceedings{WulfhorstMerseburgTippkoetter2015, author = {Wulfhorst, Helene and Merseburg, Johannes and Tippk{\"o}tter, Nils}, title = {Analyse von Lignocellulose mittels dynamischer Differenzkalorimetrie und Infrarot - Spektrometrie}, series = {12. Dresdner Sensor-Symposium 2015 2015-12-07 - 2015-12-09}, booktitle = {12. Dresdner Sensor-Symposium 2015 2015-12-07 - 2015-12-09}, isbn = {978-3-9813484-9-1}, doi = {10.5162/12dss2015/P6.2}, pages = {210 -- 215}, year = {2015}, language = {de} } @article{WulfhorstDuweMerseburgetal.2016, author = {Wulfhorst, Helene and Duwe, Anna-Maria and Merseburg, Johannes and Tippk{\"o}tter, Nils}, title = {Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis}, series = {Tetrahedron}, volume = {72}, journal = {Tetrahedron}, number = {46}, publisher = {Elsevier}, address = {Amsterdam}, doi = {10.1016/j.tet.2016.04.029}, pages = {7329 -- 7334}, year = {2016}, abstract = {The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.}, language = {en} } @inproceedings{WulfhorstDuweMoehringetal.2016, author = {Wulfhorst, H. and Duwe, A. and M{\"o}hring, S. and Jurca, O. and Tippk{\"o}tter, Nils}, title = {Analysis of pretreated biomass by differential scanning 132 calorimetry and multivariate data analysis}, series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany}, booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany}, publisher = {DECHEMA}, address = {Frankfurt am Main}, pages = {132}, year = {2016}, language = {en} } @article{WolfBerndtBrandenburg1979, author = {Wolf, Wilhelm and Berndt, Heinz and Brandenburg, Dietrich}, title = {Synthese von Fragmenten einer [LysA13] Rinder-Insulin-A-Kette unter Verwendung des S-tert-Butylmercaptorestes als Thiolschutz}, series = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, volume = {360}, journal = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, number = {2}, issn = {1437-4315}, doi = {10.1515/bchm2.1979.360.2.1549}, pages = {1549 -- 1558}, year = {1979}, language = {de} } @article{WolfBerndtBrandenburg1979, author = {Wolf, G{\"u}nter and Berndt, Heinz and Brandenburg, Dietrich}, title = {Synthese der [LysA13] Rinderinsulin-A-Kette in der Form [Lys(Tfa)A13]A(SO3H)4 und NαA1-Msc-[LysA13]A(SO3H)4 unter Verwendung des S-tert-Butylmercapto-Restes als Thiolschutzgruppe}, series = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, volume = {360}, journal = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, number = {2}, issn = {1437-4315}, doi = {10.1515/bchm2.1979.360.2.1569}, pages = {1569 -- 1578}, year = {1979}, language = {de} } @incollection{WolfKapelyukhScheeretal.2015, author = {Wolf, C. Roland and Kapelyukh, Yury and Scheer, Nico and Henderson, Colin J.}, title = {Application of Humanised and Other Transgenic Models to Predict Human Responses to Drugs}, editor = {Wilson, Alan G. E.}, publisher = {RSC Publ.}, address = {Cambridge}, isbn = {978-1-78262-778-4}, doi = {10.1039/9781782622376-00152}, pages = {152 -- 176}, year = {2015}, abstract = {The use of transgenic animal models has transformed our knowledge of complex biochemical pathways in vivo. It has allowed disease processes to be modelled and used in the development of new disease prevention and treatment strategies. They can also be used to define cell- and tissue-specific pathways of gene regulation. A further major application is in the area of preclinical development where such models can be used to define pathways of chemical toxicity, and the pathways that regulate drug disposition. One major application of this approach is the humanisation of mice for the proteins that control drug metabolism and disposition. Such models can have numerous applications in the development of drugs and in their more sophisticated use in the clinic.}, language = {en} } @article{WissenbachSixBongaertsetal.1995, author = {Wissenbach, U. and Six, S. and Bongaerts, Johannes and Ternes, D. and Steinwachs, S. and Unden, G.}, title = {A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport}, series = {Molecular microbiology}, volume = {Vol. 17}, journal = {Molecular microbiology}, number = {Iss. 4}, issn = {1365-2958 (E-Journal); 0950-382x (Print)}, pages = {675 -- 686}, year = {1995}, language = {en} } @article{WincklerKruegerSchnitzleretal.2014, author = {Winckler, Silvia and Krueger, Rolf and Schnitzler, Thomas and Zang, Werner and Fischer, Rainer and Biselli, Manfred}, title = {A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach}, series = {Bioprocess and biosystems engineering}, volume = {37}, journal = {Bioprocess and biosystems engineering}, number = {5}, publisher = {Springer}, address = {Berlin, Heidelberg}, issn = {1615-7591 (Print) 1615-7605 (Online)}, doi = {10.1007/s00449-013-1062-8}, pages = {901 -- 912}, year = {2014}, abstract = {Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.}, language = {en} } @article{WilsonWilsonScheeretal.2017, author = {Wilson, Ian D. and Wilson, Claire E. and Scheer, Nico and Dickie, A.P. and Schreiter, K. and Wilson, E. M. and Riley, R. J. and Wehr, R. and Bial, J.}, title = {The Pharmacokinetics and Metabolism of Lumiracoxib in Chimeric Humanized and Murinized FRG Mice}, series = {Biochemical pharmacology}, volume = {Volume 135}, journal = {Biochemical pharmacology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-2968}, doi = {10.1016/j.bcp.2017.03.015}, pages = {139 -- 150}, year = {2017}, language = {en} } @article{WilsonDickieSchreiteretal.2018, author = {Wilson, C. E. and Dickie, A. P. and Schreiter, K. and Wehr, R. and Wilson, E. M. and Bial, J. and Scheer, Nico and Wilson, I. D. and Riley, R. J.}, title = {The pharmacokinetics and metabolism of diclofenac in chimeric humanized and murinized FRG mice}, series = {Archives of Toxicology}, volume = {92}, journal = {Archives of Toxicology}, number = {6}, publisher = {Springer}, issn = {1432-0738}, doi = {10.1007/s00204-018-2212-1}, pages = {1953 -- 1967}, year = {2018}, abstract = {The pharmacokinetics of diclofenac were investigated following single oral doses of 10 mg/kg to chimeric liver humanized and murinized FRG and C57BL/6 mice. In addition, the metabolism and excretion were investigated in chimeric liver humanized and murinized FRG mice. Diclofenac reached maximum blood concentrations of 2.43 ± 0.9 µg/mL (n = 3) at 0.25 h post-dose with an AUCinf of 3.67 µg h/mL and an effective half-life of 0.86 h (n = 2). In the murinized animals, maximum blood concentrations were determined as 3.86 ± 2.31 µg/mL at 0.25 h post-dose with an AUCinf of 4.94 ± 2.93 µg h/mL and a half-life of 0.52 ± 0.03 h (n = 3). In C57BL/6J mice, mean peak blood concentrations of 2.31 ± 0.53 µg/mL were seen 0.25 h post-dose with a mean AUCinf of 2.10 ± 0.49 µg h/mL and a half-life of 0.51 ± 0.49 h (n = 3). Analysis of blood indicated only trace quantities of drug-related material in chimeric humanized and murinized FRG mice. Metabolic profiling of urine, bile and faecal extracts revealed a complex pattern of metabolites for both humanized and murinized animals with, in addition to unchanged parent drug, a variety of hydroxylated and conjugated metabolites detected. The profiles in humanized mice were different to those of both murinized and wild-type animals, e.g., a higher proportion of the dose was detected in the form of acyl glucuronide metabolites and much reduced amounts as taurine conjugates. Comparison of the metabolic profiles obtained from the present study with previously published data from C57BL/6J mice and humans revealed a greater, though not complete, match between chimeric humanized mice and humans, such that the liver humanized FRG model may represent a model for assessing the biotransformation of such compounds in humans.}, language = {en} } @article{WilmingBegemannKuhneetal.2013, author = {Wilming, Anja and Begemann, Jens and Kuhne, Stefan and Regestein, Lars and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and B{\"u}chs, Jochen}, title = {Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations}, series = {Biochemical engineering journal}, volume = {Vol. 73}, journal = {Biochemical engineering journal}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-295X (E-Journal); 1369-703X (Print)}, pages = {29 -- 37}, year = {2013}, language = {en} } @article{WilhelmBerndtBrandenburg1979, author = {Wilhelm, Wolff and Berndt, Heinz and Brandenburg, Dietrich}, title = {Zur Synthese der H{\"u}hnerinsulin-A-Kette, I : Darstellung der Fragmente A1-8, A9-15, A1-7 und A8-15}, series = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, volume = {360}, journal = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, number = {2}, issn = {1437-4315}, doi = {10.1515/bchm2.1979.360.2.1559}, pages = {1559 -- 1568}, year = {1979}, language = {de} } @article{WiezorekBaumann1999, author = {Wiezorek, E. and Baumann, Marcus}, title = {Stadt{\"o}kologie in der Stadtplanung: der "Stadt{\"o}kologische Beitrag" Aachen}, series = {Wechselwirkung : Wissenschaft \& vernetztes Denken}, volume = {21}, journal = {Wechselwirkung : Wissenschaft \& vernetztes Denken}, number = {97}, issn = {0172-1623}, pages = {28 -- 37}, year = {1999}, language = {de} } @article{WiesenTippkoetterMuffleretal.2014, author = {Wiesen, Sebastian and Tippk{\"o}tter, Nils and Muffler, Kai and Suck, Kirstin and Sohling, Ulrich and Ruf, Nils and Ulber, Roland}, title = {Adsorptive Vorbehandlung von Rohglycerin f{\"u}r die 1,3-Propandiol Fermentation mit Clostridium diolis}, series = {Chemie Ingenieur Technik}, volume = {86}, journal = {Chemie Ingenieur Technik}, number = {1-2}, publisher = {Wiley-VCH}, address = {Weinheim}, doi = {10.1002/cite.201300080}, pages = {129 -- 135}, year = {2014}, abstract = {Bei der Gewinnung von Fetts{\"a}uren aus Pflanzen{\"o}len, z. B. zur Herstellung von Biopolymeren, oder bei der Biodiesel- und Seifenproduktion, f{\"a}llt Glycerin als Nebenprodukt an. Bei der Biokonversion dieses Rohstoffes zu 1,3-Propandiol wird der Produktionsorganismus Clostridium diolis durch Verunreinigungen im Rohglycerin gehemmt. Als inhibierende Substanzen konnten freie Fetts{\"a}uren identifiziert werden. Mithilfe eines adsorptiven Aufarbeitungsverfahrens ist es gelungen, die Fetts{\"a}uren zu entfernen und die Konversionseffizienz zu 1,3-Propandiol zu erh{\"o}hen.}, language = {de} } @article{WiesenTippkoetterMuffleretal.2015, author = {Wiesen, Sebastian and Tippk{\"o}tter, Nils and Muffler, Kai and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland}, title = {Adsorption of fatty acids to layered double hydroxides in aqueous systems}, series = {Adsorption}, volume = {21}, journal = {Adsorption}, number = {6-7}, publisher = {Springer}, address = {Berlin}, pages = {459 -- 466}, year = {2015}, abstract = {Due to their anion exchange characteristics, layered double hydroxides (LDHs) are suitable for the detoxification of aqueous, fatty acid containing fermentation substrates. The aim of this study is to examine the adsorption mechanism, using crude glycerol from plant oil esterification as a model system. Changes in the intercalation structure in relation to the amount of fatty acids adsorbed are monitored by X-ray diffraction and infra-red spectroscopy. Additionally, calcination of LDH is investigated in order to increase the binding capacity for fatty acids. Our data propose that, at ambient temperature, fatty acids can be bound to the hydrotalcite by adsorption or in addition by intercalation, depending on fatty acid concentration. The adsorption of fatty acids from crude glycerol shows a BET-like behavior. Above a fatty acid concentration of 3.5 g L-1, intercalation of fatty acids can be shown by the appearance of an increased interlayer spacing. This observation suggests a two phase adsorption process. Calcination of LDHs allows increasing the binding capacity for fatty acids by more than six times, mainly by reduction of structural CO32-.}, language = {en} } @misc{WielandSiegertSpitzetal.2011, author = {Wieland, Susanne and Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz and O'Connell, Timothy and Pr{\"u}ser, Inken and Schiedel, Marc-Steffen and Eiting, Thomas and Sendor-M{\"u}ller, Dorota and Bastigkeit, Thorsten and Benda, Konstantin and M{\"u}ller, Sven}, title = {Lagerstabiles fl{\"u}ssiges Wasch- oder Reinigungsmittel enthaltend Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt / Europ{\"a}isches Patentamt / WIPO}, address = {M{\"u}nchen / Den Hague / Genf}, pages = {1 -- 25}, year = {2011}, language = {de} } @article{WiegandVoigtAlbrechtetal.2013, author = {Wiegand, Sandra and Voigt, Birgit and Albrecht, Dirk and Bongaerts, Johannes and Evers, Stefan and Hecker, Michael and Daniel, Rolf and Liesegang, Heiko}, title = {Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production}, series = {Microbial Cell Factories}, volume = {12}, journal = {Microbial Cell Factories}, publisher = {Biomed Central}, address = {London}, issn = {1475-2859}, doi = {10.1186/1475-2859-12-120}, pages = {120}, year = {2013}, language = {en} } @article{WiegandDietrichHerteletal.2013, author = {Wiegand, Sandra and Dietrich, Sascha and Hertel, Robert and Bongaerts, Johannes and Evers, Stefan and Volland, Sonja and Daniel, Rolf and Liesegang, Heiko}, title = {RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation}, series = {BMC genomics}, volume = {Vol. 14}, journal = {BMC genomics}, publisher = {BioMed Central}, address = {London}, issn = {1471-2164}, pages = {667}, year = {2013}, language = {en} } @article{WhiteheadOehlschlaegerAlmajhdietal.2014, author = {Whitehead, Mark and {\"O}hlschl{\"a}ger, Peter and Almajhdi, Fahad N. and Alloza, Leonor and Marz{\´a}bal, Pablo and Meyers, Ann E. and Hitzeroth, Inga I. and Rybicki, Edward P.}, title = {Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice}, series = {BMC cancer}, journal = {BMC cancer}, number = {14:367}, publisher = {BioMed Central}, address = {London}, issn = {1471-2407}, doi = {10.1186/1471-2407-14-367}, pages = {1 -- 15}, year = {2014}, language = {en} } @article{WernerKrumbeSchumacheretal.2011, author = {Werner, Frederik and Krumbe, Christoph and Schumacher, Katharina and Groebel, Simone and Spelthahn, Heiko and Stellberg, Michael and Wagner, Torsten and Yoshinobu, Tatsuo and Selmer, Thorsten and Keusgen, Michael and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1340 -- 1344}, year = {2011}, language = {en} } @article{WernerGroebelWagneretal.2011, author = {Werner, Frederik and Groebel, Simone and Wagner, Torsten and Yoshinobu, Tatsuo and Selmer, Thorsten and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {{\"U}berwachung der metabolischen Aktivit{\"a}t von Mikroorganismen zur Kontrolle des biologischen Prozesses im Biogasfermenter}, series = {Biogas 2011 : Energietr{\"a}ger der Zukunft ; 6. Fachtagung, Fachtagung Braunschweig, 08. und 09. Juni 2011 / VDI Energie und Umwelt}, journal = {Biogas 2011 : Energietr{\"a}ger der Zukunft ; 6. Fachtagung, Fachtagung Braunschweig, 08. und 09. Juni 2011 / VDI Energie und Umwelt}, publisher = {VDI-Verl.}, address = {D{\"u}sseldorf}, isbn = {978-3-18-092121-1}, pages = {285 -- 286}, year = {2011}, language = {de} } @article{WernerGroebelSchuhmacheretal.2009, author = {Werner, Frederik and Groebel, Simone and Schuhmacher, K. and Spelthahn, Heiko and Wagner, Torsten and Selmer, Thorsten and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {Bestimmung der metabolischen Aktivit{\"a}t von Mikroorganismen w{\"a}hrend des Biogasbildungsprozesses}, series = {9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]}, journal = {9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]}, publisher = {TUDpress}, address = {Dresden}, isbn = {978-3-941298-44-6}, pages = {201 -- 204}, year = {2009}, language = {de} } @article{WernerGroebelKrumbeetal.2012, author = {Werner, Frederik and Groebel, Simone and Krumbe, Christoph and Wagner, Torsten and Selmer, Thorsten and Yoshinobu, Tatsuo and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {Nutrient concentration-sensitive microorganism-based biosensor}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100801}, pages = {900 -- 904}, year = {2012}, language = {en} } @article{WerkhausenAlbrachtCroninetal.2017, author = {Werkhausen, Amelie and Albracht, Kirsten and Cronin, Neil J. and Meier, Rahel and Mojsen-Moeller, Jens and Seynnes, Olivier R.}, title = {Modulation of muscle-tendon interaction in the human triceps surae during an energy dissipation task}, series = {Journal of Experimental Biology}, volume = {220}, journal = {Journal of Experimental Biology}, number = {22}, issn = {0022-0949}, doi = {10.1242/jeb.164111}, pages = {4141 -- 4149}, year = {2017}, language = {en} } @incollection{WendorffEggertPohletal.2007, author = {Wendorff, Marion and Eggert, Thorsten and Pohl, Martina and Dresen, Carola and M{\"u}ller, Michael and Jaeger, Karl-Erich and Sprenger, Georg A. and Sch{\"u}rmann, Melanie and Sch{\"u}rmann, Martin and Johnen, Sandra and Sprenger, Gerda and Sahm, Hermann and Inoue, Tomoyuki and Sch{\"o}rken, Ulrich and Breittaupt, Holger and Fr{\"o}lich, Bettina and Heim, Petra and Iding, Hans and Juchem, Bettina and Siegert, Petra and Kula, Maria-Regina and Weckbecker, Andrea and Hummel, Werner and Fessner, Wolf-Dieter and Elling, Lothar and Wolberg, Michael and Bode, Silke and Feldmann, Ralf and Geilenkirchen, Petra and Schubert, Thomas and Walter, Lydia and D{\"u}nnwald, Thomas and Demir, Ayhan S. and Kolter-Jung, Doris and Nitsche, Adam and D{\"u}nkelmann, Pascal and Cosp, Annabel and Lingen, Bettina}, title = {Catalytic asymmetric synthesis : section 2.2}, series = {Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...}, booktitle = {Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {978-3-527-31473-7}, pages = {298 -- 413}, year = {2007}, language = {en} } @article{WeldenJablonskiWegeetal.2021, author = {Welden, Rene and Jablonski, Melanie and Wege, Christina and Keusgen, Michael and Wagner, Patrick Hermann and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase}, series = {Biosensors}, volume = {11}, journal = {Biosensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2079-6374}, doi = {10.3390/bios11060171}, pages = {Artikel 171}, year = {2021}, abstract = {The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte's pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.}, language = {en} } @article{WeldenSeverinsPoghossianetal.2022, author = {Welden, Melanie and Severins, Robin and Poghossian, Arshak and Wege, Christina and Bongaerts, Johannes and Siegert, Petra and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor}, series = {Chemosensors}, volume = {10}, journal = {Chemosensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors10060218}, pages = {Artikel 218}, year = {2022}, abstract = {Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte-insulator-semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin-streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage-current, capacitance-voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.}, language = {en} } @article{WeldenPoghossianVahidpouretal.2022, author = {Welden, Melanie and Poghossian, Arshak and Vahidpour, Farnoosh and Wendlandt, Tim and Keusgen, Michael and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers}, series = {Biosensors}, volume = {12}, journal = {Biosensors}, number = {1}, publisher = {MDPI}, address = {Basel}, issn = {2079-6374}, doi = {10.3390/bios12010043}, pages = {Artikel 43}, year = {2022}, abstract = {Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1-3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.}, language = {en} } @article{WardoyoNoorElbersetal.2020, author = {Wardoyo, Arinto Y.P. and Noor, Johan A.E. and Elbers, Gereon and Schmitz, Sandra and Flaig, Sascha T. and Budianto, Arif}, title = {Characterizing volcanic ash elements from the 2015 eruptions of bromo and raung volcanoes, Indonesia}, series = {Polish Journal of Environmental Studies}, volume = {29}, journal = {Polish Journal of Environmental Studies}, number = {2}, publisher = {HARD}, address = {Olsztyn}, issn = {2083-5906}, doi = {10.15244/pjoes/99101}, pages = {1899 -- 1907}, year = {2020}, abstract = {The volcanic eruptions of Mt. Bromo and Mt. Raung in East Java, Indonesia, in 2015 perturbed volcanic materials and affected surface-layer air quality at surrounding locations. During the episodes, the volcanic ash from the eruptions influenced visibility, traffic accidents, flight schedules, and human health. In this research, the volcanic ash particles were collected and characterized by relying on the detail of physical observation. We performed an assessment of the volcanic ash elements to characterize the volcanic ash using two different methods which are aqua regia extracts followed by MP-AES and XRF laboratory test of bulk samples. The analysis results showed that the volcanic ash was mixed of many materials, such as Al, Si, P, K, Ca, Ti, V, Cr, Mn, Fe, Ni, and others. Fe, Si, Ca, and Al were found as the major elements, while the others were the trace elements Ba, Cr, Cu, Mn, P, Mn, Ni, Zn, Sb, Sr, and V with the minor concentrations. XRF analyses showed that Fe dominated the elements of the volcanic ash. The XRF analysis showed that Fe was at 35.40\% in Bromo and 43.00\% in Raung of the detected elements in bulk material. The results of aqua regia extracts analyzed by MP-AES were 1.80\% and 1.70\% of Fe element for Bromo and Raung volcanoes, respectively.}, language = {en} } @article{WangDruckenmuellerElbersetal.2014, author = {Wang, Ren-Qi and Druckenm{\"u}ller, Katharina and Elbers, Gereon and Guenther, Klaus and Crou{\´e}, Jean-Philippe}, title = {Analysis of aquatic-phase natural organic matter by optimized LDI-MS method}, series = {Journal of mass spectrometry}, volume = {49}, journal = {Journal of mass spectrometry}, number = {2}, publisher = {Wiley}, address = {Bognor Regis}, issn = {1096-9888}, doi = {10.1002/jms.3321}, pages = {154 -- 160}, year = {2014}, abstract = {The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200-1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200-15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley \& Sons, Ltd.}, language = {en} } @article{WallerBraunHojdisetal.2007, author = {Waller, Mark P. and Braun, Heiko and Hojdis, Nils and B{\"u}hl, Michael}, title = {Geometries of Second-Row Transition-Metal Complexes from Density-Functional Theory}, series = {Journal of Chemical Theory and Computation}, volume = {3}, journal = {Journal of Chemical Theory and Computation}, number = {6}, issn = {1549-9626}, doi = {10.1021/ct700178y}, pages = {2234 -- 2242}, year = {2007}, language = {en} } @article{WagnerMolinaYoshinobuetal.2007, author = {Wagner, Torsten and Molina, Roberto and Yoshinobu, Tatsuo and Kloock, Joachim P. and Biselli, Manfred and Canzoneri, Michele and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef}, title = {Handheld multi-channel LAPS device as a transducer platform for possible biological and chemical multi-sensor applications}, series = {Electrochimica Acta. 53 (2007), H. 2}, journal = {Electrochimica Acta. 53 (2007), H. 2}, isbn = {0013-4686}, pages = {305 -- 311}, year = {2007}, language = {en} } @article{WagnerMolinaBisellietal.2007, author = {Wagner, Torsten and Molina, R. and Biselli, Manfred and Canzoneri, Michele and Schnitzler, Thomas and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {A light-addressable potentiometric sensor system for fast, simultaneous and spatial detection of the metabolic activity of biological cells}, series = {Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.)}, journal = {Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.)}, publisher = {IEEE}, address = {Piscataway}, isbn = {1-4244-0841-5}, pages = {1107 -- 1110}, year = {2007}, language = {en} } @article{WagnerBegingRotteretal.2007, author = {Wagner, Torsten and Beging, Stefan and Rotter, L. and Poghossian, Arshak and Biselli, Manfred and Zang, Werner and Sch{\"o}ning, Michael Josef}, title = {Online-Messsysteme f{\"u}r die automatisierte Charakterisierung von feldeffektbasierten Biosensoren}, series = {8. Dresdner Sensor-Symposium : Sensoren f{\"u}r Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme f{\"u}r die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung f{\"u}r die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)}, journal = {8. Dresdner Sensor-Symposium : Sensoren f{\"u}r Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme f{\"u}r die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung f{\"u}r die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)}, publisher = {TUDpress, Verl. der Wissenschaften}, address = {Dresden}, isbn = {978-3-940046-45-1}, pages = {257 -- 260}, year = {2007}, language = {de} } @incollection{WagemannTippkoetter2019, author = {Wagemann, Kurt and Tippk{\"o}tter, Nils}, title = {Biorefineries: a short introduction}, series = {Biorefineries}, booktitle = {Biorefineries}, publisher = {Springer}, address = {Cham}, isbn = {978-3-319-97117-9}, doi = {10.1007/10_2017_4}, pages = {1 -- 11}, year = {2019}, abstract = {The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown.}, language = {en} } @book{WagemannTippkoetter2019, author = {Wagemann, Kurt and Tippk{\"o}tter, Nils}, title = {Biorefineries / Kurt Wagemann, Nils Tippk{\"o}tter (editors)}, series = {Advances in biochemical engineering/biotechnology book series (ABE)}, journal = {Advances in biochemical engineering/biotechnology book series (ABE)}, publisher = {Springer}, address = {Cham (Switzerland)}, isbn = {978-3-319-97117-9}, doi = {10.1007/978-3-319-97119-3}, pages = {VI, 549 Seiten}, year = {2019}, language = {en} } @article{WackwitzBongaertsGoodmanetal.1999, author = {Wackwitz, B. and Bongaerts, Johannes and Goodman, S. D. and Unden, Gottfried}, title = {Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance}, series = {Molecular and general genetics : MGG}, volume = {Vol. 262}, journal = {Molecular and general genetics : MGG}, number = {Iss. 4 - 5}, issn = {1617-4623 (E-Journal); 1617-4615 (Print)}, pages = {876 -- 883}, year = {1999}, language = {en} } @article{VoigtSchroeterJuergenetal.2013, author = {Voigt, Birgit and Schroeter, Rebecca and J{\"u}rgen, Britta and Albrecht, Dirk and Evers, Stefan and Bongaerts, Johannes and Maurer, Karl-Heinz and Schweder, Thomas and Hecker, Michael}, title = {The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon}, series = {Proteomics}, volume = {Vol. 13}, journal = {Proteomics}, number = {Iss. 14}, publisher = {Wiley}, address = {Weinheim}, issn = {1615-9861 (E-Journal); 1615-9853 (Print)}, pages = {2140 -- 2146}, year = {2013}, language = {en} } @article{VoigtAlbrechtSieversetal.2015, author = {Voigt, Birgit and Albrecht, Dirk and Sievers, Susanne and Becher, D{\"o}rte and Bongaerts, Johannes and Evers, Stefan and Schweder, Thomas and Maurer, Karl-Heinz and Hecker, Michael}, title = {High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium}, series = {Proteomics}, volume = {15}, journal = {Proteomics}, number = {15}, publisher = {Wiley}, address = {Weinheim}, issn = {1615-9861}, doi = {10.1002/pmic.201400504}, pages = {2629 -- 2633}, year = {2015}, language = {en} } @techreport{VaessenOhmeManderscheid2012, author = {Vaeßen, Christiane and Ohme, H. and Manderscheid, D.}, title = {Endbericht Projekt Immotherm : Vorhabensbezeichnung: KMU-innovativ-Verbundvorhaben "Einsatz immobilisierter Mikroorganismen zur Ent{\"o}lung und Entsalzung von Kondensatwasser bei hohen Prozesstemperaturen" : Laufzeit des Vorhabens: 01.03.2009-31.08.2011 : F{\"o}rderkennzeichen: 01LY0816A, 01LY0816B, 01LY0816C}, pages = {71 S. : zahlr. Ill. u. graph. Darst.}, year = {2012}, language = {de} } @article{UndenBongaerts1997, author = {Unden, Gottfried and Bongaerts, Johannes}, title = {Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors}, series = {Biochimica et biophysica acta (BBA) - Bioenergetics}, volume = {Vol. 1320}, journal = {Biochimica et biophysica acta (BBA) - Bioenergetics}, number = {Iss. 3}, issn = {1879-2650 (E-Journal); 0005-2728 (Print)}, pages = {217 -- 234}, year = {1997}, language = {en} } @article{UndenBeckerBongaertsetal.1994, author = {Unden, G. and Becker, S. and Bongaerts, Johannes and Schirawski, J. and Six, S.}, title = {Oxygen regulated gene expression in facultatively anaerobic bacteria}, series = {Antonie van Leeuwenhoek}, volume = {Vol. 66}, journal = {Antonie van Leeuwenhoek}, number = {Iss. 1-3}, issn = {0003-6072 (Print) ; 1572-9699 (online)}, pages = {3 -- 22}, year = {1994}, language = {en} } @article{UndenBeckerBongaertsetal.1995, author = {Unden, G. and Becker, S. and Bongaerts, Johannes and Holighaus, G. and Schirawski, J. and Six, S.}, title = {O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria}, series = {Archives of microbiology}, volume = {Vol. 164}, journal = {Archives of microbiology}, number = {Iss. 2}, issn = {1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)}, pages = {81 -- 90}, year = {1995}, language = {en} } @article{UlberTippkoetter2009, author = {Ulber, Roland and Tippk{\"o}tter, Nils}, title = {Nitratfreie Molke}, series = {Rundschau f{\"u}r Fleischhygiene und Lebensmittel{\"u}berwachung}, journal = {Rundschau f{\"u}r Fleischhygiene und Lebensmittel{\"u}berwachung}, number = {4}, pages = {150 -- 152}, year = {2009}, language = {de} } @article{UlberPothMonzonetal.2010, author = {Ulber, Roland and Poth, Sebastian and Monzon, Magaly and Tippk{\"o}tter, Nils}, title = {Prozessintegration von Hydrolyse und Fermentation von Cellulose- Faserstoff}, series = {Chemie Ingenieur Technik}, volume = {82}, journal = {Chemie Ingenieur Technik}, number = {1-2}, issn = {1522-2640}, doi = {10.1002/cite.200900103}, pages = {135 -- 139}, year = {2010}, abstract = {Ein viel versprechender erneuerbarer Rohstoff f{\"u}r die Produktion von Chemikalien und Treibstoffen ist Lignocellulose aus pflanzlicher Biomasse. Die darin enthaltenen Zucker k{\"o}nnen mittels enzymatischer Hydrolyse freigesetzt und fermentativ zu Ethanol umgesetzt werden. Ein interessanter Ansatz ist dabei die simultane Verzuckerung und Fermentation. Hefen und Enzyme haben mit 30 °C bzw. 50 °C zwar unterschiedliche Temperaturoptima, es konnte aber gezeigt werden, dass auch bei den niedrigeren Temperaturen eine Umsetzung der Cellulose zu Glucose erfolgt, wenn auch langsamer als bei optimalen Bedingungen. Außerdem konnte in Vorversuchen gezeigt werden, dass Ethanol in den zu erwartenden Konzentrationen keinen Einfluss auf die enzymatische Umsetzung hat.}, language = {de} } @incollection{UlberMufflerTippkoetteretal.2011, author = {Ulber, Roland and Muffler, Kai and Tippk{\"o}tter, Nils and Hirth, Thomas and Sell, Dieter}, title = {Introduction to Renewable Resources in the Chemical Industry}, series = {Renewable raw materials : new feedstocks for the chemical industry}, booktitle = {Renewable raw materials : new feedstocks for the chemical industry}, editor = {Ulber, Roland and Sell, Dieter and Hirth, Thomas}, edition = {1. Auflage}, publisher = {Wiley-VCH-Verlag}, address = {Weinheim}, isbn = {978-3-527-32548-1}, pages = {1 -- 6}, year = {2011}, language = {de} } @article{UlberTippkoetterBuchholzetal.2008, author = {Ulber, R. and Tippk{\"o}tter, Nils and Buchholz, H. and Demmer, W. and Scheper, T.}, title = {Innovative Verfahren in der Molkeaufarbeitung zur Gewinnung neuer Produkte}, series = {Deutsche Milchwirtschaft}, volume = {59}, journal = {Deutsche Milchwirtschaft}, number = {19}, issn = {0012-0480}, pages = {704 -- 706}, year = {2008}, language = {de} } @article{TuegBaumann1994, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements}, series = {Geophysical research letters}, volume = {Vol. 21}, journal = {Geophysical research letters}, number = {Iss. 8}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {689 -- 692}, year = {1994}, language = {en} } @article{TuegBaumann1995, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper "Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements"}, series = {Geophysical research letters}, volume = {Vol. 22}, journal = {Geophysical research letters}, number = {Iss. 9}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {1159 -- 1160}, year = {1995}, language = {en} } @article{TurekKettererClassenetal.2007, author = {Turek, Monika and Ketterer, Lothar and Claßen, Melanie and Berndt, Heinz and Elbers, Gereon and Kr{\"u}ger, Peter and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Development and Electrochemical Investigations of an EIS-(Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection}, series = {Sensors}, volume = {7}, journal = {Sensors}, number = {8}, isbn = {1424-8220}, pages = {1415 -- 1426}, year = {2007}, language = {en} } @article{TurckBerndt1981, author = {Turck, Christoph W. and Berndt, Heinz}, title = {Synthese definierter Peptid-Derivate durch Aminolyse von 3-(Nα-Acyl-peptidyloxy)-2-hydroxy-N-alkylbenzamiden bei erh{\"o}hten Temperaturen, I : Synthese des Modellpeptid-Derivates Z-Ala-Phe-Gly-N(Et)2}, series = {Hoppe-Seyler´s Zeitschrift f{\"u}r physiologische Chemie}, volume = {362}, journal = {Hoppe-Seyler´s Zeitschrift f{\"u}r physiologische Chemie}, number = {1}, issn = {1437-4315}, doi = {10.1515/bchm2.1981.362.1.821}, pages = {821 -- 828}, year = {1981}, language = {de} } @article{TrappLammersEngudaretal.2023, author = {Trapp, Svenja and Lammers, Tom and Engudar, Gokce and Hoehr, Cornelia and Denkova, Antonia G. and Paulßen, Elisabeth and de Kruijff, Robin M.}, title = {Membrane-based microfluidic solvent extraction of Ga-68 from aqueous Zn solutions: towards an automated cyclotron production loop}, series = {EJNMMI Radiopharmacy and Chemistry}, volume = {2023}, journal = {EJNMMI Radiopharmacy and Chemistry}, number = {8, Article number: 9}, publisher = {Springer Nature}, issn = {2365-421X}, doi = {10.1186/s41181-023-00195-2}, pages = {1 -- 14}, year = {2023}, language = {en} } @article{TranBongaertsVladetal.1997, author = {Tran, Quang Hon and Bongaerts, Johannes and Vlad, Dorina and Unden, Gottfried}, title = {Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications}, series = {European journal of biochemistry}, volume = {Vol. 244}, journal = {European journal of biochemistry}, number = {Iss. 1}, issn = {0014-2956}, pages = {155 -- 160}, year = {1997}, language = {en} } @article{TippkoetterWollnySucketal.2014, author = {Tippk{\"o}tter, Nils and Wollny, Steffen and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland}, title = {Recycling of spent oil bleaching earth as source of glycerol for the anaerobic production of acetone, butanol, and ethanol with Clostridium diolis and lipolytic Clostridium lundense}, series = {Engineering in Life Sciences}, volume = {14}, journal = {Engineering in Life Sciences}, number = {4}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1618-2863}, doi = {10.1002/elsc.201300113}, pages = {425 -- 432}, year = {2014}, abstract = {A major part of edible oil is subjected to bleaching procedures, primarily with minerals applied as adsorbers. Their recycling is currently done either by regaining the oil via organic solvent extraction or by using the spent bleaching earth (SBE) as additive for animal feed, etc. As a new method, the reutilization of the by-product SBE for the microbiologic formation of acetone, butanol, and ethanol (ABE) is presented as proof-of-concept. The SBE was taken from a palm oil cleaning process. The recycling concept is based on the application of lipolytic clostridia strains. Due to considerably long fermentation times, co-fermentation with Candida rugosa and enzymatic hydrolyses of the bound oil with a subsequent clostridia fermentation are shown as alternative routes. Anaerobic fermentations under comparison of different clostridia strains were performed with glycerol media, enzymatically hydrolyzed palm oil and SBE. Solutes, side product compositions and productivities were quantified via HPLC. A successful production of ABE solutes from SBE has been done with a yield of 0.15 g butanol per gram of bound glycerol. Thus, the biotechnological recycling of the waste stream is possible in principle. Inhibition of the substrate suspension has been observed. A chromatographic ion-exchange of substrates increased the biomass concentration.}, language = {en} } @article{TippkoetterWollnyKampeisetal.2011, author = {Tippk{\"o}tter, Nils and Wollny, S. and Kampeis, P. and Oster, J. and Schneider, H. and Ulber, R.}, title = {Magnetseparation von Proteinen : Separation von Zielmolek{\"u}len durch hochselektive Aptamere}, series = {GIT Labor-Fachzeitschrift}, volume = {55}, journal = {GIT Labor-Fachzeitschrift}, number = {10}, publisher = {Wiley}, address = {Weinheim}, pages = {666}, year = {2011}, abstract = {Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinit{\"a}ten mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln wird eine einstufige Separation von Zielmolek{\"u}len aus mikrobiologischen Produktionsans{\"a}tzen m{\"o}glich. Die Aptamere werden hierf{\"u}r reversibel auf den Partikeloberfl{\"a}chen gebunden und f{\"u}r die spezifische Isolierung von Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld.}, language = {de} } @techreport{TippkoetterWagner2019, author = {Tippk{\"o}tter, Nils and Wagner, Sebastian}, title = {Biomimetischer Klebstoff aus ligninhaltigen Pflanzenresten (Teilvorhaben 1 und 2) : Schlussbericht zum Vorhaben : Laufzeit: 01.01.2016 bis 31.03.2019}, publisher = {FH Aachen}, address = {J{\"u}lich}, doi = {10.2314/KXP:169732777X}, pages = {109 S.}, year = {2019}, language = {de} } @article{TippkoetterStueckmannKrolletal.2009, author = {Tippk{\"o}tter, Nils and St{\"u}ckmann, Henning and Kroll, Stephen and Winkelmann, Gunda and Noack, Udo and Scheper, Thomas and Ulber, Roland}, title = {A semi-quantitative dipstick assay for microcystin}, series = {Analytical and Bioanalytical Chemistry}, volume = {394}, journal = {Analytical and Bioanalytical Chemistry}, number = {3}, publisher = {springer}, address = {Berlin}, issn = {1618-2650}, doi = {10.1007/s00216-009-2750-8}, pages = {863 -- 869}, year = {2009}, abstract = {An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.}, language = {en} } @inproceedings{TippkoetterStueckmannWinkelmannetal.2007, author = {Tippk{\"o}tter, Nils and St{\"u}ckmann, H. and Winkelmann, G. and Noack, U. and Beutel, S. and Scheper, T. and Ulber, R.}, title = {Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR}, series = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme}, booktitle = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme}, publisher = {Dechema}, address = {Frankfurt am Main}, pages = {126}, year = {2007}, language = {en} } @inproceedings{TippkoetterSchuenemannChristmannetal.2008, author = {Tippk{\"o}tter, Nils and Sch{\"u}nemann, V. and Christmann, R. and Pasteur, A. and Schweizer, J. and Ulber, R.}, title = {Bioaffinity Layering magnetisierbarer Mikro- und Nanopartikel}, series = {Technische Systeme f{\"u}r die Lebenswissenschaften : 14. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008}, booktitle = {Technische Systeme f{\"u}r die Lebenswissenschaften : 14. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008}, editor = {Beckmann, Dieter}, address = {Heiligenstadt}, isbn = {978-3-00-025695-0}, pages = {97 -- 98}, year = {2008}, language = {de} } @article{TippkoetterRoth2020, author = {Tippk{\"o}tter, Nils and Roth, Jasmine}, title = {Purified Butanol from Lignocellulose - Solvent-Impregnated Resins for an Integrated Selective Removal}, series = {Chemie Ingenieur Technik}, volume = {92}, journal = {Chemie Ingenieur Technik}, number = {11}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1522-2640}, doi = {10.1002/cite.202000200}, pages = {1741 -- 1751}, year = {2020}, abstract = {In traditional microbial biobutanol production, the solvent must be recovered during fermentation process for a sufficient space-time yield. Thermal separation is not feasible due to the boiling point of n-butanol. As an integrated and selective solid-liquid separation alternative, solvent impregnated resins (SIRs) were applied. Two polymeric resins were evaluated and an extractant screening was conducted. Vacuum application with vapor collection in fixed-bed column as bioreactor bypass was successfully implemented as butanol desorption step. In course of further increasing process economics, fermentation with renewable lignocellulosic substrates was conducted using Clostridium acetobutylicum. Utilization of SIR was shown to be a potential strategy for solvent removal from fermentation broth, while application of a bypass column allows for product removal and recovery at once.}, language = {en} } @article{TippkoetterRoikaewUlberetal.2010, author = {Tippk{\"o}tter, Nils and Roikaew, Wipa and Ulber, Roland and Hoffmann, Alexander and Denzler, Hans-J{\"o}rg and Buchholz, Heinrich}, title = {Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food}, series = {Biotechnology Progress}, volume = {26}, journal = {Biotechnology Progress}, number = {3}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {8756-7938}, doi = {10.1002/btpr.384}, pages = {756 -- 762}, year = {2010}, abstract = {Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed.}, language = {en} } @article{TippkoetterRoikaewUlber2008, author = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.}, title = {Nitrate removal from whey concentrate with biotechnological regeneration of the waste water}, series = {European dairy magazine : EDM}, journal = {European dairy magazine : EDM}, number = {1}, isbn = {0936-6318}, pages = {30 -- 32}, year = {2008}, language = {en} } @inproceedings{TippkoetterRoikaewUlber2008, author = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.}, title = {An automated pilot plant for the bioengineering processing of concentrated whey}, series = {European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme}, booktitle = {European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme}, publisher = {Dechema}, address = {Frankfurt am Main}, pages = {98}, year = {2008}, language = {en} } @article{TippkoetterRoikaewUlber2007, author = {Tippk{\"o}tter, Nils and Roikaew, N. and Ulber, R.}, title = {Nitratentfernung aus Molkekonzentrat mit biotechnologischer Regeneration der Abw{\"a}sser}, series = {Deutsche Milchwirtschaft}, volume = {58}, journal = {Deutsche Milchwirtschaft}, number = {15}, issn = {0012-0480}, pages = {540 -- 542}, year = {2007}, language = {de} } @incollection{TippkoetterMoehringRothetal.2019, author = {Tippk{\"o}tter, Nils and M{\"o}hring, Sophie and Roth, Jasmine and Wulfhorst, Helene}, title = {Logistics of lignocellulosic feedstocks: preprocessing as a preferable option}, series = {Biorefineries}, booktitle = {Biorefineries}, publisher = {Springer}, address = {Cham}, isbn = {978-3-319-97117-9}, doi = {10.1007/10_2017_58}, pages = {43 -- 68}, year = {2019}, abstract = {In comparison to crude oil, biorefinery raw materials are challenging in concerns of transport and storage. The plant raw materials are more voluminous, so that shredding and compacting usually are necessary before transport. These mechanical processes can have a negative influence on the subsequent biotechnological processing and shelf life of the raw materials. Various approaches and their effects on renewable raw materials are shown. In addition, aspects of decentralized pretreatment steps are discussed. Another important aspect of pretreatment is the varying composition of the raw materials depending on the growth conditions. This problem can be solved with advanced on-site spectrometric analysis of the material.}, language = {en} } @inproceedings{TippkoetterMoehringMaureretal.2013, author = {Tippk{\"o}tter, Nils and M{\"o}hring, S. and Maurer, S. and Roth, J.}, title = {Dezentrale Vorbehandlung und Verarbeitung pflanzlicher Reststoffe f{\"u}r Bioraffinerien}, series = {Kurzfassungen der Vortr{\"a}ge nach Sessions : Fr{\"u}hjahrstagung der Biotechnologen 2013, 4. - 5. M{\"a}rz 2013, Dechema-Haus, Frankfurt am Main}, booktitle = {Kurzfassungen der Vortr{\"a}ge nach Sessions : Fr{\"u}hjahrstagung der Biotechnologen 2013, 4. - 5. M{\"a}rz 2013, Dechema-Haus, Frankfurt am Main}, address = {Frankfurt am Main}, pages = {5}, year = {2013}, language = {de} } @article{TippkoetterDuweWiesenetal.2014, author = {Tippk{\"o}tter, Nils and Duwe, Anna-Maria and Wiesen, Sebastian and Sieker, Tim and Ulber, Roland}, title = {Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids}, series = {Bioresource Technology}, volume = {167}, journal = {Bioresource Technology}, publisher = {Elsevier}, address = {Amsterdam}, doi = {10.1016/j.biortech.2014.06.052}, pages = {447 -- 455}, year = {2014}, abstract = {The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10\% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9\%, 17\% and 23\% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17\% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23\% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹.}, language = {en} } @article{TippkoetterDeterdingUlber2008, author = {Tippk{\"o}tter, Nils and Deterding, A. and Ulber, Roland}, title = {Determination of acetic acid in fermentation broth by gas-diffusion technique}, series = {Engineering in Life Sciences}, volume = {8}, journal = {Engineering in Life Sciences}, number = {1, Special Issue: Technical Systems for the Use in Life Sciences}, doi = {10.1002/elsc.200820227}, pages = {62 -- 67}, year = {2008}, abstract = {Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5-5.0 g/L acetic acid with a relative standard deviation of <5 \% was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.}, language = {en} } @article{TippkoetterAlKaidyWollnyetal.2013, author = {Tippk{\"o}tter, Nils and Al-Kaidy, Huschyar and Wollny, Steffen and Ulber, Roland}, title = {Functionalized magnetizable particles for downstream processing in single-use systems}, series = {Chemie Ingenieur Technik}, volume = {85}, journal = {Chemie Ingenieur Technik}, number = {1-2: Special Issue: Single-Use Technology}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/cite.201200130}, pages = {76 -- 86}, year = {2013}, abstract = {Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential.}, language = {en} } @incollection{Tippkoetter2016, author = {Tippk{\"o}tter, Nils}, title = {Grundlagen der bio-chemischen Umwandlung}, series = {Energie aus Biomasse : Grundlagen, Techniken und Verfahren}, booktitle = {Energie aus Biomasse : Grundlagen, Techniken und Verfahren}, editor = {Kaltschmidt, Martin}, edition = {3., aktualisierte, erweiterte Auflage}, publisher = {Springer Vieweg}, address = {Berlin ; Heidelberg}, isbn = {978-3-662-47437-2 (Print)}, doi = {10.1007/978-3-662-47438-9}, pages = {1447 -- 1500}, year = {2016}, language = {de} } @book{Tippkoetter2010, author = {Tippk{\"o}tter, Nils}, title = {Reaktionssysteme zur Aufarbeitung und Umsetzung nachwachsender Rohstoffe : Einsatz chromatographischer Verfahren sowie Membran- und Festbettreaktoren zur Verarbeitung von Molke, St{\"a}rke und Cellulose}, publisher = {Logos-Verlag}, address = {Berlin}, isbn = {978-3-8325-2717-4}, pages = {III, 269 Seiten}, year = {2010}, language = {de} } @inproceedings{Tippkoetter2013, author = {Tippk{\"o}tter, Nils}, title = {Biotechnologische Gewinnung von Wertstoffen aus Molke : BiobasedWorld - Innovation in food}, series = {Biotechnica 2013 : European biotechnology science \& industry news}, volume = {12}, booktitle = {Biotechnica 2013 : European biotechnology science \& industry news}, number = {9, special}, pages = {33 -- 50}, year = {2013}, language = {de} } @techreport{Tippkoetter2018, author = {Tippk{\"o}tter, Nils}, title = {Lokale Vorbehandlung nachwachsender Rohstoffe f{\"u}r Bioraffinerien (BioSats) : Schlussbericht zum Vorhaben : Laufzeit: 01.03.2012 bis 30.04.2017}, organization = {Technische Universit{\"a}t Kaiserslautern}, doi = {10.2314/GBV:1024204243}, pages = {191 Seiten}, year = {2018}, language = {de} } @article{ThielTippkoetterSucketal.2013, author = {Thiel, Alexander and Tippk{\"o}tter, Nils and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland}, title = {New zeolite adsorbents for downstream processing of polyphenols from renewable resources}, series = {Engineering in Life Sciences}, volume = {13}, journal = {Engineering in Life Sciences}, number = {3}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/elsc.201200188}, pages = {239 -- 246}, year = {2013}, abstract = {Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5\% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100\% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources.}, language = {en} } @article{ThielMufflerTippkoetteretal.2015, author = {Thiel, Alexander and Muffler, Kai and Tippk{\"o}tter, Nils and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland}, title = {Aufarbeitung von Polyphenolen aus Weizen mittels Zeolithen am Beispiel der Ferulas{\"a}ure}, series = {Chemie IngenieurTechnik}, volume = {87}, journal = {Chemie IngenieurTechnik}, number = {1-2}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/cite.201400031}, pages = {128 -- 136}, year = {2015}, abstract = {Aufarbeitung von Polyphenolen aus Weizenmittels Zeolithen am Beispiel der Ferulasa¨ ureAlexander Thiel1, Kai Muffler1, Nils Tippko¨ tter1, Kirstin Suck2, Ulrich Sohling2, Friedrich Ruf3und Roland Ulber1,*DOI: 10.1002/cite.201400031Bei der Ferulasa¨ure handelt es sich um einen Wertstoff, der aus Weizen gewonnen und in der Lebensmittel- und Pharma-industrie eingesetzt werden kann. Der Einsatz von Weizen als nachwachsende Rohstoffquelle ist allerdings nur dann wirt-schaftlich durchfu¨hrbar, wenn eine Prozessintegration in die bestehenden industriellen Verfahren gewa¨hrleistet oder einedirekte Konkurrenz zur Mehl- und Sta¨rkeindustrie vermieden werden kann. In diesem Artikel wird ein Verfahren aufge-zeigt, welches hohe Ausbeuten ermo¨glicht und eine Konkurrenz zu bestehenden Verwertungspfaden vermeidet.}, language = {de} } @article{ThielMufflerTippkoetteretal.2015, author = {Thiel, Alexander and Muffler, Kai and Tippk{\"o}tter, Nils and Suck, Kirstin and Sohling, Ulrich and Hruschka, Steffen M. and Ulber, Roland}, title = {A novel integrated downstream processing approach to recover sinapic acid, phytic acid and proteins from rapeseed meal}, series = {Journal of Chemical Technology and Biotechnology}, volume = {90}, journal = {Journal of Chemical Technology and Biotechnology}, number = {11}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/jctb.4664}, pages = {1999 -- 2006}, year = {2015}, abstract = {BACKGROUND Currently, several techniques exist for the downstream processing of protein, phytic acid and sinapic acid from rapeseed and rapeseed meal, but no technique has been developed to separate all of the components in one process. In this work, two new downstream processing strategies focusing on recovering sinapic acid, phytic acid and protein from rapeseed meal were established. RESULTS The sinapic acid content was enhanced by a factor of 4.5 with one method and 5.1 with the other. The isolation of sinapic acid was accomplished using a zeolite-based adsorbent with high adsorptive and optimal desorption characteristics. Phytic acid was isolated using the anion-exchange resin Purolite A200®. In addition, the processes resulted in two separated protein fractions. The ratios of globulin and albumin ratio to the total protein were 59.2\% and 40.1\%, respectively. The steps were then combined in two different ways: (a) a 'sequential process' using the zeolite and A200 in batch processes; and (b) a 'parallel process' using only A200 in a chromatographic system to separate all of the compounds. CONCLUSIONS It can be concluded that isolation of all three components was possible in both processes. These could enhance the added value of current processes using rapeseed meal as a protein source. © 2015 Society of Chemical Industry}, language = {en} } @article{TeumerCapitainRossJonesetal.2018, author = {Teumer, T. and Capitain, C. and Ross-Jones, J. and Tippk{\"o}tter, Nils and R{\"a}dle, M. and Methner, F.-J.}, title = {In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor}, series = {BrewingScience}, volume = {71}, journal = {BrewingScience}, number = {5/6}, publisher = {Fachverlag Hans Carl}, address = {N{\"u}rnberg}, issn = {1613-2041}, pages = {49 -- 55}, year = {2018}, abstract = {In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented.}, language = {en} } @phdthesis{TemizArtmann2001, author = {Temiz Artmann, Ayseg{\"u}l}, title = {Sicanlarda egzersiz sonrasi oksidatif hasar ve eritrosit membran degisikliklerinin hemoreolojik etkileri}, year = {2001}, language = {mul} } @phdthesis{TemizArtmann1996, author = {Temiz Artmann, Ayseg{\"u}l}, title = {Sicanlarda, akut egzersiz sonucu gelisen oksidan stresin l{\"o}kosit aktivasyon degisiklikleri ile ile olan iliskisi ve eritrosit deformabilitesine etkisi}, year = {1996}, language = {mul} } @article{TakenagaSchneiderErbayetal.2015, author = {Takenaga, Shoko and Schneider, Benno and Erbay, E. and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and Wagner, Torsten}, title = {Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201532053}, pages = {1347 -- 1352}, year = {2015}, abstract = {A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.}, language = {en} } @inproceedings{TakenagaHerreraWerneretal.2013, author = {Takenaga, Shoko and Herrera, Cony F. and Werner, Frederik and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten}, title = {Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip}, series = {11. Dresdner Sensor-Symposium : 9.-11.12.2013}, booktitle = {11. Dresdner Sensor-Symposium : 9.-11.12.2013}, organization = {Dresdner Sensor-Symposium <11, 2013>}, isbn = {978-3-9813484-5-3}, pages = {63 -- 67}, year = {2013}, language = {en} } @article{TakenagaBiselliSchnitzleretal.2014, author = {Takenaga, Shoko and Biselli, Manfred and Schnitzler, Thomas and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing}, series = {Physica status solidi A : Applications and materials science}, volume = {211}, journal = {Physica status solidi A : Applications and materials science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330464}, pages = {1410 -- 1415}, year = {2014}, abstract = {The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.}, language = {en} } @article{SvaneborgKarimiVarzanehHojdisetal.2016, author = {Svaneborg, Carsten and Karimi-Varzaneh, Hossein Ali and Hojdis, Nils and Fleck, Franz and Everaers, Ralf}, title = {Multiscale approach to equilibrating model polymer melts}, series = {Physical Review E}, volume = {94}, journal = {Physical Review E}, number = {032502}, publisher = {AIP Publishing}, address = {Melville, NY}, issn = {2470-0053}, doi = {10.1103/PhysRevE.94.032502}, year = {2016}, abstract = {We present an effective and simple multiscale method for equilibrating Kremer Grest model polymer melts of varying stiffness. In our approach, we progressively equilibrate the melt structure above the tube scale, inside the tube and finally at the monomeric scale. We make use of models designed to be computationally effective at each scale. Density fluctuations in the melt structure above the tube scale are minimized through a Monte Carlo simulated annealing of a lattice polymer model. Subsequently the melt structure below the tube scale is equilibrated via the Rouse dynamics of a force-capped Kremer-Grest model that allows chains to partially interpenetrate. Finally the Kremer-Grest force field is introduced to freeze the topological state and enforce correct monomer packing. We generate 15 melts of 500 chains of 10.000 beads for varying chain stiffness as well as a number of melts with 1.000 chains of 15.000 monomers. To validate the equilibration process we study the time evolution of bulk, collective, and single-chain observables at the monomeric, mesoscopic, and macroscopic length scales. Extension of the present method to longer, branched, or polydisperse chains, and/or larger system sizes is straightforward.}, language = {en} } @article{SvaneborgKarimiVarzanehHojdisetal.2018, author = {Svaneborg, Carsten and Karimi-Varzaneh, Hossein Ali and Hojdis, Nils and Fleck, Franz and Everaers, Ralf}, title = {Kremer-Grest Models for Universal Properties of Specific Common Polymer Species}, series = {Soft Condensed Matter}, journal = {Soft Condensed Matter}, number = {1606.05008}, year = {2018}, abstract = {The Kremer-Grest (KG) bead-spring model is a near standard in Molecular Dynamic simulations of generic polymer properties. It owes its popularity to its computational efficiency, rather than its ability to represent specific polymer species and conditions. Here we investigate how to adapt the model to match the universal properties of a wide range of chemical polymers species. For this purpose we vary a single parameter originally introduced by Faller and M{\"u}ller-Plathe, the chain stiffness. Examples include polystyrene, polyethylene, polypropylene, cis-polyisoprene, polydimethylsiloxane, polyethyleneoxide and styrene-butadiene rubber. We do this by matching the number of Kuhn segments per chain and the number of Kuhn segments per cubic Kuhn volume for the polymer species and for the Kremer-Grest model. We also derive mapping relations for converting KG model units back to physical units, in particular we obtain the entanglement time for the KG model as function of stiffness allowing for a time mapping. To test these relations, we generate large equilibrated well entangled polymer melts, and measure the entanglement moduli using a static primitive-path analysis of the entangled melt structure as well as by simulations of step-strain deformation of the model melts. The obtained moduli for our model polymer melts are in good agreement with the experimentally expected moduli.}, language = {en} } @misc{StellbergJeromin2000, author = {Stellberg, Michael and Jeromin, G{\"u}nter Erich}, title = {R{\"u}hrvorrichtung f{\"u}r Magnetr{\"u}hrsystem : Patentschrift DE19807518C2 ; Ver{\"o}ffentlichungstag der Patenterteilung: 31.05.2000}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {14 S. : Ill.}, year = {2000}, language = {de} } @article{StanleyHorsburghRossetal.2009, author = {Stanley, Lesley A. and Horsburgh, Brian C. and Ross, Jillian and Scheer, Nico and Wolf, C. Roland}, title = {Drug transporters: Gatekeepers controlling access of xenobiotics to the cellular interior}, series = {Drug Metabolism Reviews}, volume = {41}, journal = {Drug Metabolism Reviews}, number = {1}, publisher = {Taylor \& Francis}, address = {London}, issn = {1097-9883}, doi = {10.1080/03602530802605040}, pages = {27 -- 65}, year = {2009}, language = {en} } @article{StanleyHorsburghRossetal.2006, author = {Stanley, Lesley A. and Horsburgh, Brian C. and Ross, Jillian and Scheer, Nico and Wolf, C. Roland}, title = {Nuclear Receptors which play a pivotal role in drug disposition and chemical toxicity}, series = {Drug Metabolism Reviews}, volume = {38}, journal = {Drug Metabolism Reviews}, number = {3}, issn = {1097-9883}, doi = {10.1080/03602530600786232}, pages = {515 -- 597}, year = {2006}, language = {en} }