@article{ScheerRossRodeetal.2008, author = {Scheer, Nico and Ross, Jillian and Rode, Anja and Zevnik, Branko and Niehaves, Sandra and Faust, Nicole and Wolf, C. Roland}, title = {A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response}, series = {Journal of Clinical Investigation}, volume = {118}, journal = {Journal of Clinical Investigation}, number = {9}, issn = {1558-8238}, doi = {https://doi.org/10.1172/JCI35483}, pages = {3228 -- 3239}, year = {2008}, language = {en} } @article{SelmerFiguraSchmidtetal.1998, author = {Selmer, Thorsten and Figura, Kurt von and Schmidt, Bernhard and Dierks, T.}, title = {A novel protein modification generating an aldehyde group in sulfatases: its role in catalysis and disease / Figura, Kurt von ; Schmidt, Bernhard ; Selmer, Thorsten ; Dierks, Thomas}, series = {Bioessays. 20 (1998), H. 6}, journal = {Bioessays. 20 (1998), H. 6}, isbn = {1521-1878}, pages = {505 -- 510}, year = {1998}, language = {en} } @article{ScheerRiedlWarrenetal.2002, author = {Scheer, Nico and Riedl, Iris and Warren, J.T. and Kuwada, John Y. and Campos-Ortega, Jos{\´e} A.}, title = {A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish}, series = {Mechanism of Development}, volume = {112}, journal = {Mechanism of Development}, number = {1-2}, issn = {0925-4773}, doi = {10.1016/S0925-4773(01)00621-9}, pages = {9 -- 14}, year = {2002}, language = {en} } @article{TippkoetterStueckmannKrolletal.2009, author = {Tippk{\"o}tter, Nils and St{\"u}ckmann, Henning and Kroll, Stephen and Winkelmann, Gunda and Noack, Udo and Scheper, Thomas and Ulber, Roland}, title = {A semi-quantitative dipstick assay for microcystin}, series = {Analytical and Bioanalytical Chemistry}, volume = {394}, journal = {Analytical and Bioanalytical Chemistry}, number = {3}, publisher = {springer}, address = {Berlin}, issn = {1618-2650}, doi = {10.1007/s00216-009-2750-8}, pages = {863 -- 869}, year = {2009}, abstract = {An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.}, language = {en} } @article{WincklerKruegerSchnitzleretal.2014, author = {Winckler, Silvia and Krueger, Rolf and Schnitzler, Thomas and Zang, Werner and Fischer, Rainer and Biselli, Manfred}, title = {A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach}, series = {Bioprocess and biosystems engineering}, volume = {37}, journal = {Bioprocess and biosystems engineering}, number = {5}, publisher = {Springer}, address = {Berlin, Heidelberg}, issn = {1615-7591 (Print) 1615-7605 (Online)}, doi = {10.1007/s00449-013-1062-8}, pages = {901 -- 912}, year = {2014}, abstract = {Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.}, language = {en} } @article{WissenbachSixBongaertsetal.1995, author = {Wissenbach, U. and Six, S. and Bongaerts, Johannes and Ternes, D. and Steinwachs, S. and Unden, G.}, title = {A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport}, series = {Molecular microbiology}, volume = {Vol. 17}, journal = {Molecular microbiology}, number = {Iss. 4}, issn = {1365-2958 (E-Journal); 0950-382x (Print)}, pages = {675 -- 686}, year = {1995}, language = {en} } @article{SelmerThamerCirpusetal.2003, author = {Selmer, Thorsten and Thamer, Wiebke and Cirpus, Irina and Hans, Marcus}, title = {A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans / Thamer, Wiebke ; Cirpus, Irina ; Hans, Marcus ; Pierik, Antonio, J. ; Selmer, Thorsten ; Bill, Eckhard ;}, series = {Archives of Microbiology. 179 (2003), H. 3}, journal = {Archives of Microbiology. 179 (2003), H. 3}, isbn = {1432-072X}, pages = {197 -- 204}, year = {2003}, language = {en} } @article{MangHodeniusSchmitzRodeetal.2009, author = {Mang, Thomas and Hodenius, M. A. J. and Schmitz-Rode, T. and Baumann, M.}, title = {Absorption of 10-hydroxycamptothecin into the coat of magnetoliposomes / Hodenius, M. A. J. ; Schmitz-Rode, T. ; Baumann, M. ; Ivoanova, G. ; Wong, J. E. ; Mang, T. ; Haulena, F. ; Soenen, S. J. H. ; De Cuyper, M.}, series = {Colloids and Surfaces A: Physicochemical and Engineering Aspects. 343 (2009), H. 1-3}, journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects. 343 (2009), H. 1-3}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0927-7757}, pages = {20 -- 23}, year = {2009}, language = {en} } @article{SelmerHetzelBrocketal.2003, author = {Selmer, Thorsten and Hetzel, Marc and Brock, Matthias and Pierik, Antonio J.}, title = {Acryloyl-CoA reductase from Clostridium propionicum. An enzyme complex of propionyl-CoA dehydrogenase and electron-transferring flavoprotein / Hetzel, Marc ; Brock, Matthias ; Selmer, Thorsten, Pierik, Antonio J. ; Golding, Bernard T. ; Buckel, Wolfgang}, series = {European Journal of Biochemistry. 270 (2003), H. 5}, journal = {European Journal of Biochemistry. 270 (2003), H. 5}, isbn = {0014-2956}, pages = {902 -- 910}, year = {2003}, language = {en} } @article{PohlSiegertMeschetal.1998, author = {Pohl, Martina and Siegert, Petra and Mesch, K. and Bruhn, H. and Gr{\"o}tzinger, Joachim}, title = {Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482}, series = {European journal of biochemistry}, volume = {Vol. 257}, journal = {European journal of biochemistry}, number = {Iss. 3}, issn = {1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print)}, pages = {538 -- 546}, year = {1998}, language = {en} } @article{SelmerBrueserDahl2000, author = {Selmer, Thorsten and Br{\"u}ser, Thomas and Dahl, Christiane}, title = {ADP Sulfurylase" from Thiobacillus denitrificans Is an Adenylylsulfate:Phosphate Adenylyltransferase and Belongs to a New Family of Nucleotidyltransferases / Br{\"u}ser, Thomas ; Selmer, Thorsten ; Dahl, Christiane}, series = {Journal of Biological Chemistry. 275 (2000), H. 3}, journal = {Journal of Biological Chemistry. 275 (2000), H. 3}, isbn = {1083-351X}, pages = {1691 -- 1690}, year = {2000}, language = {en} } @article{LauthChristmannSchwarz1990, author = {Lauth, Jakob and Christmann, K. and Schwarz, E.}, title = {Adsorption geometry of H on RU(1010) image as determined by LEED and HREELS / Christmann, K. ; Lauth, G. ; Schwarz, E.}, series = {Vacuum. 41 (1990), H. 1-3}, journal = {Vacuum. 41 (1990), H. 1-3}, isbn = {0042-207X}, pages = {293 -- 296}, year = {1990}, language = {en} } @article{WiesenTippkoetterMuffleretal.2015, author = {Wiesen, Sebastian and Tippk{\"o}tter, Nils and Muffler, Kai and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland}, title = {Adsorption of fatty acids to layered double hydroxides in aqueous systems}, series = {Adsorption}, volume = {21}, journal = {Adsorption}, number = {6-7}, publisher = {Springer}, address = {Berlin}, pages = {459 -- 466}, year = {2015}, abstract = {Due to their anion exchange characteristics, layered double hydroxides (LDHs) are suitable for the detoxification of aqueous, fatty acid containing fermentation substrates. The aim of this study is to examine the adsorption mechanism, using crude glycerol from plant oil esterification as a model system. Changes in the intercalation structure in relation to the amount of fatty acids adsorbed are monitored by X-ray diffraction and infra-red spectroscopy. Additionally, calcination of LDH is investigated in order to increase the binding capacity for fatty acids. Our data propose that, at ambient temperature, fatty acids can be bound to the hydrotalcite by adsorption or in addition by intercalation, depending on fatty acid concentration. The adsorption of fatty acids from crude glycerol shows a BET-like behavior. Above a fatty acid concentration of 3.5 g L-1, intercalation of fatty acids can be shown by the appearance of an increased interlayer spacing. This observation suggests a two phase adsorption process. Calcination of LDHs allows increasing the binding capacity for fatty acids by more than six times, mainly by reduction of structural CO32-.}, language = {en} } @article{ZhantlessovaSavitskayaKistaubayevaetal.2022, author = {Zhantlessova, Sirina and Savitskaya, Irina and Kistaubayeva, Aida and Ignatova, Ludmila and Talipova, Aizhan and Pogrebnjak, Alexander and Digel, Ilya}, title = {Advanced "Green" prebiotic composite of bacterial cellulose/pullulan based on synthetic biology-powered microbial coculture strategy}, series = {Polymers}, volume = {14}, journal = {Polymers}, number = {15}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym14153224}, pages = {Artikel 3224}, year = {2022}, abstract = {Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.}, language = {en} } @article{HendersonScheerWolf2009, author = {Henderson, Colin J. and Scheer, Nico and Wolf, C. Roland}, title = {Advances in the generation of mouse models to elucidate the pathways of drug metabolism in rodents and man}, series = {Expert Review of Clinical Pharmacology}, volume = {2}, journal = {Expert Review of Clinical Pharmacology}, number = {2}, publisher = {Taylor \& Francis}, address = {London}, issn = {1751-2441}, doi = {10.1586/17512433.2.2.105}, pages = {105 -- 109}, year = {2009}, language = {en} } @article{ElbersPfefferFrieseletal.1995, author = {Elbers, Gereon and Pfeffer, Hans-Ulrich and Friesel, J{\"u}rgen and Beier, Reinhold}, title = {Air Pollution Monitoring in Street Canyons / Hans-Ulrich Pfeffer, J{\"u}rgen Friesel, Gereon Elbers, Reinhold Beier,}, series = {Transport and air pollution : proceedings ; 3rd international symposium, Palais des Papes, Avignon, France, 6 - 10 June 1994 = Transports et pollution de l'air / [Third International Symposium on Transport and Air Pollution]. Ed. by Robert Joumard. Organizer: French National Institute for Transport and Safety Research}, journal = {Transport and air pollution : proceedings ; 3rd international symposium, Palais des Papes, Avignon, France, 6 - 10 June 1994 = Transports et pollution de l'air / [Third International Symposium on Transport and Air Pollution]. Ed. by Robert Joumard. Organizer: French National Institute for Transport and Safety Research}, publisher = {Elsevier}, address = {Amsterdam}, pages = {IX, 337 S : graph. Darst.}, year = {1995}, language = {en} } @article{RoeschKratzHeringetal.2016, author = {R{\"o}sch, C. and Kratz, F. and Hering, T. and Trautmann, S. and Umanskaya, N. and Tippk{\"o}tter, Nils and M{\"u}ller-Renno, C.M. and Ulber, Roland and Hannig, M. and Ziegler, C.}, title = {Albumin-lysozyme interactions: cooperative adsorption on titanium and enzymatic activity}, series = {Colloids and Surfaces B: Biointerfaces}, volume = {149}, journal = {Colloids and Surfaces B: Biointerfaces}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, doi = {10.1016/j.colsurfb.2016.09.048}, pages = {115 -- 121}, year = {2016}, abstract = {The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase.}, language = {en} } @article{JerominScheidt1991, author = {Jeromin, G{\"u}nter Erich and Scheidt, Annette}, title = {Aliphatic optically active alcohols by enzyme-aided synthesis}, series = {Tetrahedron Letters . 32 (1991), H. 48}, journal = {Tetrahedron Letters . 32 (1991), H. 48}, isbn = {0040-4039}, pages = {7021 -- 7024}, year = {1991}, language = {en} } @article{PrielmeierWickNagatomoetal.1995, author = {Prielmeier, Franz and Wick, Markus and Nagatomo, Yasushi and Frahm, Jens}, title = {Alteration of Intracellular Metabolite Diffusion in Rat Brain In Vivo During Ischemia and Reperfusion / Markus Wick, Yasushi Nagatomo, Franz Prielmeier, Jens Frahm}, series = {Stroke. 26 (1995), H. 10}, journal = {Stroke. 26 (1995), H. 10}, isbn = {0039-2499}, pages = {1930 -- 1934}, year = {1995}, language = {en} } @article{UndenBongaerts1997, author = {Unden, Gottfried and Bongaerts, Johannes}, title = {Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors}, series = {Biochimica et biophysica acta (BBA) - Bioenergetics}, volume = {Vol. 1320}, journal = {Biochimica et biophysica acta (BBA) - Bioenergetics}, number = {Iss. 3}, issn = {1879-2650 (E-Journal); 0005-2728 (Print)}, pages = {217 -- 234}, year = {1997}, language = {en} } @article{BiselliBornThoemmesetal.1996, author = {Biselli, Manfred and Born, C. and Th{\"o}mmes, J. and Wandrey, C.}, title = {An approach to integrated antibody production: Coupling of fluidized bed cultivation and fluidized bed adsorption / Born, C. ; Th{\"o}mmes, J. ; Biselli, M. ; Wandrey, C. ; Kula, M.-R.}, series = {Bioprocess Engineering. 15 (1996), H. 1}, journal = {Bioprocess Engineering. 15 (1996), H. 1}, isbn = {1615-7591}, pages = {21 -- 29}, year = {1996}, language = {en} } @article{SpiessWilfriedAlvarezetal.2011, author = {Spiess, Elmar and Wilfried, Reichardt and Alvarez, Gerardo and Gottrup, Marcus and {\"O}hlschl{\"a}ger, Peter}, title = {An Artificial PAP Gene Breaks Self-tolerance and Promotes Tumor Regression in the TRAMP Model for Prostate Carcinoma}, series = {Molecular Therapy}, volume = {20}, journal = {Molecular Therapy}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1525-0016}, pages = {555 -- 564}, year = {2011}, language = {en} } @article{NiedermeyerZhouDursunetal.2016, author = {Niedermeyer, Angela and Zhou, Bei and Dursun, G{\"o}zde and Temiz Artmann, Ayseg{\"u}l and Markert, Bernd}, title = {An examination of tissue engineered scaffolds in a bioreactor}, series = {Proceedings in Applied Mathematics and Mechanics PAMM}, volume = {16}, journal = {Proceedings in Applied Mathematics and Mechanics PAMM}, number = {1}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1617-7061}, doi = {10.1002/pamm.201610038}, pages = {99 -- 100}, year = {2016}, abstract = {Replacement tissues, designed to fill in articular cartilage defects, should exhibit the same properties as the native material. The aim of this study is to foster the understanding of, firstly, the mechanical behavior of the material itself and, secondly, the influence of cultivation parameters on cell seeded implants as well as on cell migration into acellular implants. In this study, acellular cartilage replacement material is theoretically, numerically and experimentally investigated regarding its viscoelastic properties, where a phenomenological model for practical applications is developed. Furthermore, remodeling and cell migration are investigated.}, language = {en} } @article{ScheerHendersonKapelyukhetal.2019, author = {Scheer, Nico and Henderson, Colin James and Kapelyukh, Yury and Rode, Anja and Mclaren, Aileen W. and MacLeod, Alastair Kenneth and Lin, De and Wright, Jayne and Stanley, Lesley and Wolf, C. Roland}, title = {An extensively humanised mouse model to predict pathways of drug disposition, drug/drug interactions, and to facilitate the design of clinical trials}, series = {Drug Metabolism and Disposition}, journal = {Drug Metabolism and Disposition}, number = {Early view}, doi = {10.1124/dmd.119.086397}, pages = {69 Seiten}, year = {2019}, language = {en} } @article{OehlschlaegerPesOsenetal.2006, author = {{\"O}hlschl{\"a}ger, Peter and Pes, Michaela and Osen, Wolfram and D{\"u}rst, Matthias}, title = {An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response / {\"O}hlschl{\"a}ger, Peter ; Pes, Michaela ; Osen, Wolfram ; D{\"u}rst, Matthias ; Schneider, Achim ; Gissmann, Lutz ; Kaufman}, series = {Vaccine. 24 (2006), H. 15}, journal = {Vaccine. 24 (2006), H. 15}, isbn = {0264-410X}, pages = {2880 -- 2893}, year = {2006}, language = {en} } @article{SchiffelsPinkenburgScheldenetal.2013, author = {Schiffels, Johannes and Pinkenburg, Olaf and Schelden, Maximilian and Aboulnaga, El-Hussiny A. A. and Baumann, Marcus and Selmer, Thorsten}, title = {An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli}, series = {PLOS one. 2013}, journal = {PLOS one. 2013}, publisher = {Public Library of Science}, address = {San Francisco, California}, issn = {1932-6203}, doi = {10.1371/journal.pone.0068812}, year = {2013}, language = {en} } @article{ScheerGrothHansetal.2001, author = {Scheer, Nico and Groth, Anne and Hans, Stefan and Campos-Ortega, Jos{\´e} A.}, title = {An instructive function for Notch in promoting gliogenesis in the zebrafish retina}, series = {Development}, volume = {128}, journal = {Development}, number = {7}, issn = {0950-1991}, pages = {1099 -- 1107}, year = {2001}, language = {en} } @article{WangDruckenmuellerElbersetal.2014, author = {Wang, Ren-Qi and Druckenm{\"u}ller, Katharina and Elbers, Gereon and Guenther, Klaus and Crou{\´e}, Jean-Philippe}, title = {Analysis of aquatic-phase natural organic matter by optimized LDI-MS method}, series = {Journal of mass spectrometry}, volume = {49}, journal = {Journal of mass spectrometry}, number = {2}, publisher = {Wiley}, address = {Bognor Regis}, issn = {1096-9888}, doi = {10.1002/jms.3321}, pages = {154 -- 160}, year = {2014}, abstract = {The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200-1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200-15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley \& Sons, Ltd.}, language = {en} } @article{GhoschBaierSchuetzetal.2016, author = {Ghosch, S. and Baier, M. and Sch{\"u}tz, J. and Schneider, Felix and Scherer, Ulrich W.}, title = {Analysis of electronic autoradiographs by mathematical post-processing}, series = {Radiation Effects and Defects in Solids: Incorporating plasma science and plasma technology}, volume = {171}, journal = {Radiation Effects and Defects in Solids: Incorporating plasma science and plasma technology}, number = {1-2}, publisher = {Taylor \& Francis}, address = {London}, issn = {1029-4953}, doi = {10.1080/10420150.2016.1155587}, pages = {161 -- 172}, year = {2016}, abstract = {Autoradiography is a well-established method of nuclear imaging. When different radionuclides are present simultaneously, additional processing is needed to distinguish distributions of radionuclides. In this work, a method is presented where aluminium absorbers of different thickness are used to produce images with different cut-off energies. By subtracting images pixel-by-pixel one can generate images representing certain ranges of β-particle energies. The method is applied to the measurement of irradiated reactor graphite samples containing several radionuclides to determine the spatial distribution of these radionuclides within pre-defined energy windows. The process was repeated under fixed parameters after thermal treatment of the samples. The greyscale images of the distribution after treatment were subtracted from the corresponding pre-treatment images. Significant changes in the intensity and distribution of radionuclides could be observed in some samples. Due to the thermal treatment parameters the most significant differences were observed in the ³H and ¹⁴C inventory and distribution.}, language = {en} } @article{BiselliKlyushnichenkoRodenbrocketal.1998, author = {Biselli, Manfred and Klyushnichenko, Vadim and Rodenbrock, Anja and Th{\"o}mmes, J{\"o}rg}, title = {Analysis of Hybridoma Cell Culture Processes by SDS-Gel Capillary Electrophoresis and matrix-assisted laser desorption ionization-time-of-flight MS / Vadim Klyushnichenko, Anja Rodenbrock, J{\"o}rg Th{\"o}mmes, Maria-Regina Kula, Holger Heine, Manfred Biselli}, series = {Biotechnology and Applied Biochemistry. 27 (1998), H. 3}, journal = {Biotechnology and Applied Biochemistry. 27 (1998), H. 3}, isbn = {0885-4513}, pages = {181 -- 188}, year = {1998}, language = {en} } @article{SchererSantanaMaieretal.2009, author = {Scherer, Ulrich W. and Santana, H. H. S. and Maier, G. and Rodenas, J.}, title = {Analysis of mechanical strength in ceramic pellets of nuclear fuel / Santana, H. H. S. ; Maier, G. ; Scherer, U. W. ; Rodenas, J.}, series = {Radiation effects and defects in solids. 164 (2009), H. 5-6}, journal = {Radiation effects and defects in solids. 164 (2009), H. 5-6}, publisher = {Taylor \& Francis}, address = {London}, isbn = {1042-0150}, pages = {313 -- 318}, year = {2009}, language = {en} } @article{PilasYaziciSelmeretal.2018, author = {Pilas, Johanna and Yazici, Y. and Selmer, Thorsten and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Application of a portable multi-analyte biosensor for organic acid determination in silage}, series = {Sensors}, volume = {18}, journal = {Sensors}, number = {5}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s18051470}, pages = {12 Seiten}, year = {2018}, abstract = {Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at -21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at -21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media.}, language = {en} } @article{IdingSiegertMeschetal.1998, author = {Iding, Hans and Siegert, Petra and Mesch, K. and Pohl, Martina}, title = {Application of α-keto acid decarboxylases in biotransformations}, series = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology}, volume = {Vol. 1385}, journal = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology}, number = {Iss. 2}, issn = {1879-2588 (E-Journal); 0167-4838 (Print)}, pages = {307 -- 322}, year = {1998}, language = {en} } @article{OjovanSteinmetz2022, author = {Ojovan, Michael I. and Steinmetz, Hans-J{\"u}rgen}, title = {Approaches to Disposal of Nuclear Waste}, series = {Energies}, volume = {15}, journal = {Energies}, number = {20}, publisher = {MDPI}, address = {Basel}, issn = {1996-1073}, doi = {10.3390/en15207804}, pages = {Artikel 7804}, year = {2022}, abstract = {We present a concise mini overview on the approaches to the disposal of nuclear waste currently used or deployed. The disposal of nuclear waste is the end point of nuclear waste management (NWM) activities and is the emplacement of waste in an appropriate facility without the intention to retrieve it. The IAEA has developed an internationally accepted classification scheme based on the end points of NWM, which is used as guidance. Retention times needed for safe isolation of waste radionuclides are estimated based on the radiotoxicity of nuclear waste. Disposal facilities usually rely on a multi-barrier defence system to isolate the waste from the biosphere, which comprises the natural geological barrier and the engineered barrier system. Disposal facilities could be of a trench type, vaults, tunnels, shafts, boreholes, or mined repositories. A graded approach relates the depth of the disposal facilities' location with the level of hazard. Disposal practices demonstrate the reliability of nuclear waste disposal with minimal expected impacts on the environment and humans.}, language = {en} } @article{AlKaidyKuthanHeringetal.2016, author = {Al-Kaidy, Huschyar and Kuthan, Kai and Hering, Thomas and Tippk{\"o}tter, Nils}, title = {Aqueous droplets used as enzymatic microreactors and their electromagnetic actuation}, series = {Journal of Visualized Experiments}, journal = {Journal of Visualized Experiments}, number = {Issue 126}, issn = {1940-087X}, doi = {10.3791/54643}, year = {2016}, abstract = {For the successful implementation of microfluidic reaction systems, such as PCR and electrophoresis, the movement of small liquid volumes is essential. In conventional lab-on-a-chip-platforms, solvents and samples are passed through defined microfluidic channels with complex flow control installations. The droplet actuation platform presented here is a promising alternative. With it, it is possible to move a liquid drop (microreactor) on a planar surface of a reaction platform (lab-in-a-drop). The actuation of microreactors on the hydrophobic surface of the platform is based on the use of magnetic forces acting on the outer shell of the liquid drops which is made of a thin layer of superhydrophobic magnetite particles. The hydrophobic surface of the platform is needed to avoid any contact between the liquid core and the surface to allow a smooth movement of the microreactor. On the platform, one or more microreactors with volumes of 10 µL can be positioned and moved simultaneously. The platform itself consists of a 3 x 3 matrix of electrical double coils which accommodate either neodymium or iron cores. The magnetic field gradients are automatically controlled. By variation of the magnetic field gradients, the microreactors' magnetic hydrophobic shell can be manipulated automatically to move the microreactor or open the shell reversibly. Reactions of substrates and corresponding enzymes can be initiated by merging the microreactors or bringing them into contact with surface immobilized catalysts.}, language = {en} } @article{SchererSchaedelBruechleetal.1989, author = {Scherer, Ulrich W. and Sch{\"a}del, M. and Br{\"u}chle, W. and J{\"a}ger, E.}, title = {ARCA II - A New Apparatus for Fast Repetitive HPLC-Separations / M. Sch{\"a}del, W. Br{\"u}chle, E. J{\"a}ger, E. Schimpf, J.V. Kratz, U.W. Scherer, H.P. Zimmermann}, series = {Radiochimica Acta. 48 (1989)}, journal = {Radiochimica Acta. 48 (1989)}, isbn = {0033-8230}, pages = {171}, year = {1989}, language = {en} } @article{SchererHoer1997, author = {Scherer, Ulrich W. and H{\"o}r, G.}, title = {Artifacts and Pitfalls in FDG-PET Whole-Body Scans / U.W. Scherer, G. H{\"o}r}, series = {Radionuclides for Mammary Gland - Current Status and Future Aspects / G. S. Limouris [Hrsg.]}, journal = {Radionuclides for Mammary Gland - Current Status and Future Aspects / G. S. Limouris [Hrsg.]}, publisher = {Mediterra Publishers}, address = {Athen}, isbn = {960-85227-6-5}, pages = {37 -- 42}, year = {1997}, language = {en} } @article{PaulssenKongArciszewskietal.2012, author = {Paulßen, Elisabeth and Kong, Shushu and Arciszewski, Pawel and Wielbalck, Swantje and Abram, Ulrich}, title = {Aryl and NHC Compounds of Technetium and Rhenium}, series = {Journal of the American Chemical Society}, volume = {134}, journal = {Journal of the American Chemical Society}, number = {22}, publisher = {ACS Publications}, address = {Washington, DC}, issn = {1520-5126}, doi = {10.1021/ja3033718}, pages = {9118 -- 9121}, year = {2012}, abstract = {Air- and water-stable phenyl complexes with nitridotechnetium(V) cores can be prepared by straightforward procedures. [TcNPh2(PPh3)2] is formed by the reaction of [TcNCl2(PPh3)2] with PhLi. The analogous N-heterocyclic carbene (NHC) compound [TcNPh2(HLPh)2], where HLPh is 1,3,4-triphenyl-1,2,4-triazol-5-ylidene, is available from (NBu4)[TcNCl4] and HLPh or its methoxo-protected form. The latter compound allows the comparison of different Tc-C bonds within one compound. Surprisingly, the Tc chemistry with such NHCs does not resemble that of corresponding Re complexes, where CH activation and orthometalation dominate.}, language = {en} } @article{SelmerMiechDierksetal.1998, author = {Selmer, Thorsten and Miech, Claudia and Dierks, Thomas and Figura, Kurt von}, title = {Arylsulfatase from Klebsiella pneumoniae Carries a Formylglycine Generated from a Serine / Miech, Claudia ; Dierks, Thomas ; Selmer, Thorsten ; Figura, Kurt von ; Schmidt, Bernd}, series = {Journal of Biological Chemistry. 273 (1998), H. 9}, journal = {Journal of Biological Chemistry. 273 (1998), H. 9}, isbn = {1083-351X}, pages = {4835 -- 4837}, year = {1998}, language = {en} } @article{InfantinoPaulssenMostaccietal.2016, author = {Infantino, Angelo and Paulßen, Elisabeth and Mostacci, Domiziano and Schaffer, Paul and Trinczek, Michael and Hoehr, Cornelia}, title = {Assessment of the production of medical isotopes using the Monte Carlo code FLUKA: Simulations against experimental measurements}, series = {Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms}, volume = {366}, journal = {Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-9584}, doi = {10.1016/j.nimb.2015.10.067}, pages = {117 -- 123}, year = {2016}, abstract = {The Monte Carlo code FLUKA is used to simulate the production of a number of positron emitting radionuclides, ¹⁸F, ¹³N, ⁹⁴Tc, ⁴⁴Sc, ⁶⁸Ga, ⁸⁶Y, ⁸⁹Zr, ⁵²Mn, ⁶¹Cu and ⁵⁵Co, on a small medical cyclotron with a proton beam energy of 13 MeV. Experimental data collected at the TR13 cyclotron at TRIUMF agree within a factor of 0.6 ± 0.4 with the directly simulated data, except for the production of ⁵⁵Co, where the simulation underestimates the experiment by a factor of 3.4 ± 0.4. The experimental data also agree within a factor of 0.8 ± 0.6 with the convolution of simulated proton fluence and cross sections from literature. Overall, this confirms the applicability of FLUKA to simulate radionuclide production at 13 MeV proton beam energy.}, language = {en} } @article{ReugelsBoggettiScheeretal.2006, author = {Reugels, Alexander M. and Boggetti, Barbara and Scheer, Nico and Campos-Ortega, Jos{\´e} A.}, title = {Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio}, series = {Developmental Dynamics}, volume = {235}, journal = {Developmental Dynamics}, number = {4}, issn = {1097-0177}, doi = {10.1002/dvdy.20699}, pages = {934 -- 948}, year = {2006}, language = {en} } @article{BiselliVanderPolDeGooijeretal.1996, author = {Biselli, Manfred and Van der Pol, Jens J. and De Gooijer, Cornelis D. and Wandrey, Christian}, title = {Automation of selective assays for on-line bioprocess monitoring by flow-injection analysis / van der Pol, Jens J. ; de Gooijer, Cornelis D. ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes}, series = {Trends in Biotechnology. 14 (1996), H. 12}, journal = {Trends in Biotechnology. 14 (1996), H. 12}, isbn = {0167-7799}, pages = {471 -- 477}, year = {1996}, language = {en} } @article{HandtkeSchroeterJuergenetal.2014, author = {Handtke, Stefan and Schroeter, Rebecca and J{\"u}rgen, Britta and Methling, Karen and Schl{\"u}ter, Rabea and Albrecht, Dirk and Hijum, Sacha A. F. T. van and Bongaerts, Johannes and Maurer, Karl-Heinz and Lalk, Michael and Schweder, Thomas and Hecker, Michael and Voigt, Birgit}, title = {Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress}, series = {PLOS one}, volume = {9}, journal = {PLOS one}, number = {1}, publisher = {PLOS}, address = {San Francisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0085625}, pages = {e85625}, year = {2014}, abstract = {Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or B. licheniformis. In this study the response of B. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen peroxide at the proteome, transcriptome and metabolome level. Genes/proteins belonging to regulons, which are known to have important functions in the oxidative stress response of other organisms, were found to be upregulated, such as the Fur, Spx, SOS or CtsR regulon. Strikingly, parts of the fundamental PerR regulon responding to peroxide stress in B. subtilis are not encoded in the B. pumilus genome. Thus, B. pumilus misses the catalase KatA, the DNA-protection protein MrgA or the alkyl hydroperoxide reductase AhpCF. Data of this study suggests that the catalase KatX2 takes over the function of the missing KatA in the oxidative stress response of B. pumilus. The genome-wide expression analysis revealed an induction of bacillithiol (Cys-GlcN-malate, BSH) relevant genes. An analysis of the intracellular metabolites detected high intracellular levels of this protective metabolite, which indicates the importance of bacillithiol in the peroxide stress resistance of B. pumilus.}, language = {en} } @article{SelmerJennemannBaueretal.1999, author = {Selmer, Thorsten and Jennemann, Richard and Bauer, Bernhard L. and Bertalanffy, Helmut}, title = {Basidiolipids from Agaricus are novel immune adjuvants / Jennemann, R. ; Bauer, BL. ; Bertalanffy, H. ; Selmer, T. ; Wiegandt, H.}, series = {Immunobiology. 200 (1999), H. 2}, journal = {Immunobiology. 200 (1999), H. 2}, isbn = {0171-2985}, pages = {277 -- 289}, year = {1999}, language = {en} } @article{BiselliThoemmesKulaetal.1994, author = {Biselli, Manfred and Th{\"o}mmes, J. and Kula, M.-R. and Wandrey, Christian}, title = {Batch kinetic data of hybridoma growth and productivity as a basis for the simulation of antibody production in various culture systems / Th{\"o}mmes, J. ; Biselli, M. ; Kula, M.-R. ; Wandrey, C.}, series = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750618450}, pages = {513 -- 517}, year = {1994}, language = {en} } @article{BurgerLindnerRumpfetal.2022, author = {Burger, Ren{\´e} and Lindner, Simon and Rumpf, Jessica and Do, Xuan Tung and Diehl, Bernd W.K. and Rehahn, Matthias and Monakhova, Yulia and Schulze, Margit}, title = {Benchtop versus high field NMR: Comparable performance found for the molecular weight determination of lignin}, series = {Journal of Pharmaceutical and Biomedical Analysis}, volume = {212}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, number = {Article number: 114649}, publisher = {Elsevier}, address = {New York, NY}, isbn = {0731-7085}, doi = {10.1016/j.jpba.2022.114649}, year = {2022}, abstract = {Lignin is a promising renewable biopolymer being investigated worldwide as an environmentally benign substitute of fossil-based aromatic compounds, e.g. for the use as an excipient with antioxidant and antimicrobial properties in drug delivery or even as active compound. For its successful implementation into process streams, a quick, easy, and reliable method is needed for its molecular weight determination. Here we present a method using 1H spectra of benchtop as well as conventional NMR systems in combination with multivariate data analysis, to determine lignin's molecular weight (Mw and Mn) and polydispersity index (PDI). A set of 36 organosolv lignin samples (from Miscanthus x giganteus, Paulownia tomentosa and Silphium perfoliatum) was used for the calibration and cross validation, and 17 samples were used as external validation set. Validation errors between 5.6\% and 12.9\% were achieved for all parameters on all NMR devices (43, 60, 500 and 600 MHz). Surprisingly, no significant difference in the performance of the benchtop and high-field devices was found. This facilitates the application of this method for determining lignin's molecular weight in an industrial environment because of the low maintenance expenditure, small footprint, ruggedness, and low cost of permanent magnet benchtop NMR systems.}, language = {en} } @article{IdingDuennwaldGreineretal.2000, author = {Iding, Hans and D{\"u}nnwald, Thomas and Greiner, Lasse and Liese, Andreas and M{\"u}ller, Michael and Siegert, Petra and Gr{\"o}tzinger, Joachim and Demir, Ayhan S. and Pohl, Martina}, title = {Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones}, series = {Chemistry - a European journal}, volume = {Vol. 6}, journal = {Chemistry - a European journal}, number = {Iss. 8}, issn = {1521-3765 (E-Journal); 0947-6539 (Print)}, pages = {1483 -- 1495}, year = {2000}, language = {en} } @article{SelmerNetzPohletal.2002, author = {Selmer, Thorsten and Netz, Daili Jacqueline Aguilar and Pohl, Regula and Beck-Sickinger, Annette G.}, title = {Biochemical characterisation and genetic analysis of aureocin A53, a new, atypical bacteriocin from Staphylococcus aureus / Netz, Daili Jacqueline Aguilar ; Pohl, Regula ; Beck-Sickinger, Annette G. ; Selmer, Thorsten ; Pierik, Antonio J. ; Carmo de Frei}, series = {Journal of Molecular Biology. 319 (2002), H. 3}, journal = {Journal of Molecular Biology. 319 (2002), H. 3}, isbn = {0022-2836}, pages = {745 -- 756}, year = {2002}, language = {en} } @article{MaggakisKelemenBorkKayseretal.2003, author = {Maggakis-Kelemen, C. and Bork, M. and Kayser, Peter and Biselli, Manfred and Artmann, Gerhard}, title = {Biological and mechanical quality of red blood cells cultured from human umbilical cord blood stem cells}, series = {Medical and biological engineering and computing. 41 (2003), H. 3}, journal = {Medical and biological engineering and computing. 41 (2003), H. 3}, isbn = {0140-0118}, pages = {350 -- 356}, year = {2003}, language = {en} } @article{BiselliHilbertJelineketal.2001, author = {Biselli, Manfred and Hilbert, U. and Jelinek, N. and Schmidt, S.}, title = {Bioprocess development for the cultivation of human T-lymphocytes / Hilbert, U. ; Jelinek, N. Schmidt, S. ; Biselli, M.}, series = {Engineering in Life Sciences. 1 (2001)}, journal = {Engineering in Life Sciences. 1 (2001)}, isbn = {1618-0240}, pages = {20 -- 23}, year = {2001}, language = {en} } @article{AlKaidyDuweHusteretal.2015, author = {Al-Kaidy, Huschyar and Duwe, Anna and Huster, Manuel and Muffler, Kai and Schlegel, Christin and Tim, Sieker and Stadtm{\"u}ller, Ralf and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Biotechnology and bioprocess engineering - from the first ullmann's article to recent trends}, series = {ChemBioEng Reviews}, volume = {2}, journal = {ChemBioEng Reviews}, number = {3}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/cben.201500008}, pages = {175 -- 184}, year = {2015}, abstract = {For several thousand years, biotechnology and its associated technical processes have had a great impact on the development of mankind. Based on empirical methods, in particular for the production of foodstuffs and daily commodities, these disciplines have become one of the most innovative future issues. Due to the increasing detailed understanding of cellular processes, production strains can now be optimized. In combination with modern bioprocesses, a variety of bulk and fine chemicals as well as pharmaceuticals can be produced efficiently. In this article, some of the current trends in biotechnology are discussed.}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{HandtkeVollandMethlingetal.2014, author = {Handtke, Stefan and Volland, Sonja and Methling, Karen and Albrecht, Dirk and Becher, D{\"o}rte and Nehls, Jenny and Bongaerts, Johannes and Maurer, Karl-Heinz and Lalk, Michael and Liesegang, Heiko and Voigt, Birgit and Daniel, Rolf and Hecker, Michael}, title = {Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach}, series = {Journal of Biotechnology}, journal = {Journal of Biotechnology}, number = {192(A)}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2014.08.028}, pages = {204 -- 214}, year = {2014}, abstract = {Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43\% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC-MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.}, language = {en} } @article{KochWunderlichSeibleretal.2008, author = {Koch, Linda and Wunderlich, F. Thomas and Seibler, Jost and K{\"o}nner, A. Christine and Hampel, Brigitte and Irlenbusch, Sigrid and Brabant, Georg and Kahn, C. Ronald and Schwenk, Frieder and Br{\"u}ning, Jens C.}, title = {Central insulin action regulates peripheral glucose and fat metabolism in mice}, series = {The Journal of Clinical Investigation (JCI)}, volume = {118}, journal = {The Journal of Clinical Investigation (JCI)}, number = {6}, issn = {1558-8238}, doi = {10.1172/JCI31073}, pages = {2132 -- 2147}, year = {2008}, language = {en} } @article{PrielmeierNagatomoFrahm1994, author = {Prielmeier, Franz and Nagatomo, Yasushi and Frahm, Jens}, title = {Cerebral blood oxygenation in rat brain during hypoxic hypoxia. Quantitative MRI of effective transverse relaxation rates}, series = {Magnetic Resonance in Medicine. 31 (1994), H. 6}, journal = {Magnetic Resonance in Medicine. 31 (1994), H. 6}, isbn = {0740-3194}, pages = {678 -- 681}, year = {1994}, language = {en} } @article{Lassonczyk1990, author = {Lassonczyk, Beate}, title = {Characteristics of a fossil soil developed on the basement complex in the northeast Somalia}, series = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, journal = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, isbn = {0172-8784}, pages = {713 -- 718}, year = {1990}, language = {en} } @article{SelmerKimDarleyetal.2006, author = {Selmer, Thorsten and Kim, Jihoe and Darley, Daniel and Buckel, Wolfgang}, title = {Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile / Kim, J. ; Darley, D. ; Selmer, T. ; Buckel, W.}, series = {Applied and Environmental Microbiology. 72 (2006), H. 9}, journal = {Applied and Environmental Microbiology. 72 (2006), H. 9}, isbn = {0099-2240}, pages = {6062 -- 6069}, year = {2006}, language = {en} } @article{JossekBongaertsSprenger2001, author = {Jossek, Ralf and Bongaerts, Johannes and Sprenger, Georg A.}, title = {Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli}, series = {FEMS microbiology letters}, volume = {Vol. 202}, journal = {FEMS microbiology letters}, number = {Iss. 1}, issn = {1574-6968}, pages = {145 -- 148}, year = {2001}, language = {en} } @article{BiselliRechmannFriedrichetal.2007, author = {Biselli, Manfred and Rechmann, Hanrik and Friedrich, Andrea and Forouzan, Dara}, title = {Characterization of photosynthetically active duckweed ( Wolffia australiana ) in vitro culture by Respiration Activity Monitoring System (RAMOS) / Henrik Rechmann, Andrea Friedrich, Dara Forouzan, Stefan Barth, Heide Schnabl, Manfred Biselli und Robert B}, series = {Biotechnology Letters. 29 (2007), H. 6}, journal = {Biotechnology Letters. 29 (2007), H. 6}, isbn = {0141-5492}, pages = {971 -- 977}, year = {2007}, language = {en} } @article{WardoyoNoorElbersetal.2020, author = {Wardoyo, Arinto Y.P. and Noor, Johan A.E. and Elbers, Gereon and Schmitz, Sandra and Flaig, Sascha T. and Budianto, Arif}, title = {Characterizing volcanic ash elements from the 2015 eruptions of bromo and raung volcanoes, Indonesia}, series = {Polish Journal of Environmental Studies}, volume = {29}, journal = {Polish Journal of Environmental Studies}, number = {2}, publisher = {HARD}, address = {Olsztyn}, issn = {2083-5906}, doi = {10.15244/pjoes/99101}, pages = {1899 -- 1907}, year = {2020}, abstract = {The volcanic eruptions of Mt. Bromo and Mt. Raung in East Java, Indonesia, in 2015 perturbed volcanic materials and affected surface-layer air quality at surrounding locations. During the episodes, the volcanic ash from the eruptions influenced visibility, traffic accidents, flight schedules, and human health. In this research, the volcanic ash particles were collected and characterized by relying on the detail of physical observation. We performed an assessment of the volcanic ash elements to characterize the volcanic ash using two different methods which are aqua regia extracts followed by MP-AES and XRF laboratory test of bulk samples. The analysis results showed that the volcanic ash was mixed of many materials, such as Al, Si, P, K, Ca, Ti, V, Cr, Mn, Fe, Ni, and others. Fe, Si, Ca, and Al were found as the major elements, while the others were the trace elements Ba, Cr, Cu, Mn, P, Mn, Ni, Zn, Sb, Sr, and V with the minor concentrations. XRF analyses showed that Fe dominated the elements of the volcanic ash. The XRF analysis showed that Fe was at 35.40\% in Bromo and 43.00\% in Raung of the detected elements in bulk material. The results of aqua regia extracts analyzed by MP-AES were 1.80\% and 1.70\% of Fe element for Bromo and Raung volcanoes, respectively.}, language = {en} } @article{BaumannJahnke1983, author = {Baumann, Marcus and Jahnke, J.}, title = {Chemical and Physical Effects on the Shape and Growth of the Diatom Biddulphia sinensis GREVILLE in Batch Cultures: A Contribution to Bioindication in Plankton Ecology / Jahnke, J. ; Baumann, M.}, series = {Hydrobiological Bulletin . 17 (1983), H. 1}, journal = {Hydrobiological Bulletin . 17 (1983), H. 1}, isbn = {0165-1404}, pages = {5 -- 20}, year = {1983}, language = {en} } @article{SchererZimmermannGoberetal.1993, author = {Scherer, Ulrich W. and Zimmermann, H. P. and Gober, M. K. and Kratz, J. V.}, title = {Chemical Properties of Element 105 in Aqueous Solution: Back Extraction from Triisooctyl Amine into 0.5M HCl / H.P. Zimmermann, M.K. Gober, J.V. Kratz, M. Sch{\"a}del, E. Schimpf, K.E. Gregorich, A. T{\"u}rler, K.R. Czerwinski, N.J. Hannink, B. Kadkhodayan, D.M.}, series = {Radiochimica Acta. 60 (1993)}, journal = {Radiochimica Acta. 60 (1993)}, isbn = {0033-8230}, pages = {11}, year = {1993}, language = {en} } @article{SchererSchaedelBruechleetal.1992, author = {Scherer, Ulrich W. and Sch{\"a}del, M. and Br{\"u}chle, W. and Schimpf, E.}, title = {Chemical Properties of Element 105 in Aqueous Solution: Cation Exchange Separations with \&\#945;-Hydroxyisobutyric Acid / M. Sch{\"a}del, W. Br{\"u}chle, E. Schimpf, H.P. Zimmermann, M.K. Gober, J.V. Kratz, N. Trautmann, H. G{\"a}ggeler, D. Jost, J. Kovacs, U.W. Sche}, series = {Radiochimica Acta. 57 (1992)}, journal = {Radiochimica Acta. 57 (1992)}, isbn = {0033-8230}, pages = {85 -- 92}, year = {1992}, language = {en} } @article{SchererGoberKratzetal.1992, author = {Scherer, Ulrich W. and Gober, M. K. and Kratz, J. V. and Zimmermann, H. P.}, title = {Chemical Properties of Element 105 in Aqueous Solution: Extractions into Diisobutylcarbinol / M.K. Gober, J.V. Kratz, H.P. Zimmermann, M. Sch{\"a}del, W. Br{\"u}chle, E. Schimpf, K.E. Gregorich, A. T{\"u}rler, N.J. Hannink, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee,}, series = {Radiochimica Acta. 57 (1992)}, journal = {Radiochimica Acta. 57 (1992)}, isbn = {0033-8230}, pages = {77 -- 84}, year = {1992}, language = {en} } @article{SchererKratzZimmermannetal.1989, author = {Scherer, Ulrich W. and Kratz, J. V. and Zimmermann, H. P. and Sch{\"a}del, M.}, title = {Chemical Properties of Element 105 in Aqueous Solutions: Halide Complex Formation and Anion Exchange into Triisooctylamine / J.V. Kratz, H.P. Zimmermann, U.W. Scherer, M. Sch{\"a}del, W. Br{\"u}chle, K.E. Gregorich, C.M. Gannett, H.L. Hall, R.A. Henderson, D.M. L}, series = {Radiochimica Acta. 48 (1989)}, journal = {Radiochimica Acta. 48 (1989)}, isbn = {0033-8230}, pages = {121}, year = {1989}, language = {en} } @article{DuennwaldDemirSiegertetal.2001, author = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael}, title = {ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation}, series = {Cheminform}, volume = {Vol. 32}, journal = {Cheminform}, number = {Iss. 4}, issn = {1522-2667 (E-Journal); 0931-7597 (Print)}, pages = {Publ. online}, year = {2001}, language = {en} } @article{BaeckerRakowskiPoghossianetal.2013, author = {B{\"a}cker, Matthias and Rakowski, D. and Poghossian, Arshak and Biselli, Manfred and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis}, series = {Journal of Biotechnology}, volume = {163}, journal = {Journal of Biotechnology}, number = {4}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0168-1656}, doi = {10.1016/j.jbiotec.2012.03.014}, pages = {371 -- 376}, year = {2013}, abstract = {A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.}, language = {en} } @article{KuropkaMuellerHoeckeretal.1989, author = {Kuropka, Rolf and M{\"u}ller, Bettina and H{\"o}cker, Hartwig and Berndt, Heinz}, title = {Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers}, series = {Journal of chromatography A}, journal = {Journal of chromatography A}, number = {481}, isbn = {0021-9673}, pages = {380 -- 386}, year = {1989}, language = {en} } @article{ZhangHeimbachScheeretal.2016, author = {Zhang, Jin and Heimbach, Tycho and Scheer, Nico and Barve, Avantika and Li, Wenkui and Lin, Wen and He, Handan}, title = {Clinical Exposure Boost Predictions by Integrating Cytochrome P450 3A4-Humanized Mouse Studies With PBPK Modeling}, series = {Journal of Pharmaceutical Sciences}, volume = {Volume 105}, journal = {Journal of Pharmaceutical Sciences}, number = {Issue 4}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0022-3549}, doi = {doi.org/10.1016/j.xphs.2016.01.021}, pages = {1398 -- 1404}, year = {2016}, abstract = {NVS123 is a poorly water-soluble protease 56 inhibitor in clinical development. Data from in vitro hepatocyte studies suggested that NVS123 is mainly metabolized by CYP3A4. As a consequence of limited solubility, NVS123 therapeutic plasma exposures could not be achieved even with high doses and optimized formulations. One approach to overcome NVS123 developability issues was to increase plasma exposure by coadministrating it with an inhibitor of CYP3A4 such as ritonavir. A clinical boost effect was predicted by using physiologically based pharmacokinetic (PBPK) modeling. However, initial boost predictions lacked sufficient confidence because a key parameter, fraction of drug metabolized by CYP3A4 (ƒₘCYP3A4), could not be estimated with accuracy on account of disconnects between in vitro and in vivo preclinical data. To accurately estimate ƒₘCYP3A4 in human, an in vivo boost effect study was conducted using CYP3A4-humanized mouse model which showed a 33- to 56-fold exposure boost effect. Using a top-down approach, human ƒₘCYP3A4 for NVS123 was estimated to be very high and included in the human PBPK modeling to support subsequent clinical study design. The combined use of the in vivo boost study in CYP3A4-humanized mouse model mice along with PBPK modeling accurately predicted the clinical outcome and identified a significant NVS123 exposure boost (∼42-fold increase) with ritonavir.}, language = {en} } @article{EngelGemuendeHoltmannetal.2019, author = {Engel, Mareike and Gem{\"u}nde, Andre and Holtmann, Dirk and M{\"u}ller-Renno, Christine and Ziegler, Christiane and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Clostridium acetobutylicum's connecting world: cell appendage formation in bioelectrochemical systems}, series = {ChemElectroChem}, volume = {7}, journal = {ChemElectroChem}, number = {2}, publisher = {Wiley}, address = {Weinheim}, issn = {2196-0216}, doi = {10.1002/celc.201901656}, pages = {414 -- 420}, year = {2019}, abstract = {Bacterial cell appendix formation supports cell-cell interaction, cell adhesion and cell movement. Additionally, in bioelectrochemical systems (BES), cell appendages have been shown to participate in extracellular electron transfer. In this work, the cell appendix formation of Clostridium acetobutylicum in biofilms of a BES are imaged and compared with conventional biofilms. Under all observed conditions, the cells possess filamentous appendages with a higher number and density in the BES. Differences in the amount of extracellular polymeric substance in the biofilms of the electrodes lead to the conclusion that the cathode can be used as electron donor and the anode as electron acceptor by C. acetobutylicum. When using conductive atomic force microscopy, a current response of about 15 nA is found for the cell appendages from the BES. This is the first report of conductivity for clostridial cell appendices and represents the basis for further studies on their role for biofilm formation and electron transfer.}, language = {en} } @article{BiselliSchroederHerfurthetal.1997, author = {Biselli, Manfred and Schr{\"o}der, B. and Herfurth, C. and Schmoll, H.-J.}, title = {Cocultivation of hematopoietic cells in a fluidized bed reactor / Schr{\"o}der, B. ; Herfurth, C. ; Biselli, M. ; Schmoll, H.-J. ; Link, H. ; Ebell, W. ; Wandrey, C.}, series = {Animal cell technology : basic \& applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita}, journal = {Animal cell technology : basic \& applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita}, publisher = {Kluwer Acad. Press}, address = {Boston}, isbn = {0-7923-4486-3}, pages = {137 -- 141}, year = {1997}, language = {en} } @article{SchererGaeggelerJostetal.1989, author = {Scherer, Ulrich W. and G{\"a}ggeler, H. W. and Jost, D. T. and T{\"u}rler, A.}, title = {Cold Fusion Reactions with 48Ca / H.W. G{\"a}ggeler, D.T. Jost, A. T{\"u}rler, P. Armbruster, W. Br{\"u}chle, H. Folger, F.P. Heßberger, S. Hofmann, G. M{\"u}nzenberg, V. Ninov, W. Reisdorf, M. Sch{\"a}del, K. S{\"u}mmerer, J.V. Kratz, U. Scherer, M.E. Leino}, series = {Nuclear Physics A . 502 (1989), H. 1}, journal = {Nuclear Physics A . 502 (1989), H. 1}, isbn = {0375-9474}, pages = {561 -- 570}, year = {1989}, language = {en} } @article{KapplerTanudyayaSchmittTippkoetteretal.2007, author = {Kappler-Tanudyaya, Nathalie and Schmitt, Heike and Tippk{\"o}tter, Nils and Meyer, Lina and Lenzen, Sigurd and Ulber, Roland}, title = {Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose}, series = {Biotechnology Journal}, volume = {2}, journal = {Biotechnology Journal}, number = {6}, issn = {1860-7314}, doi = {10.1002/biot.200700004}, pages = {692 -- 699}, year = {2007}, abstract = {Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.}, language = {en} } @article{SchiffelsSelmer2019, author = {Schiffels, Johannes and Selmer, Thorsten}, title = {Combinatorial assembly of ferredoxin-linked modules in Escherichia coli yields a testing platform for Rnf-complexes}, series = {Biotechnology and Bioengineering}, journal = {Biotechnology and Bioengineering}, number = {accepted article}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/bit.27079}, pages = {1 -- 36}, year = {2019}, language = {en} } @article{BalakrishnanAndreiSelmerSelmeretal.2010, author = {Balakrishnan, Karthikeyan and Andrei-Selmer, Luminita-Cornelia and Selmer, Thorsten and Bacher, Michael and Dodel, Richard}, title = {Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β}, series = {Journal of Alzheimer's Disease}, volume = {20}, journal = {Journal of Alzheimer's Disease}, number = {1}, isbn = {1387-2877}, pages = {135 -- 143}, year = {2010}, language = {en} } @article{WulfhorstDuweMerseburgetal.2016, author = {Wulfhorst, Helene and Duwe, Anna-Maria and Merseburg, Johannes and Tippk{\"o}tter, Nils}, title = {Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis}, series = {Tetrahedron}, volume = {72}, journal = {Tetrahedron}, number = {46}, publisher = {Elsevier}, address = {Amsterdam}, doi = {10.1016/j.tet.2016.04.029}, pages = {7329 -- 7334}, year = {2016}, abstract = {The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.}, language = {en} } @article{HagerHentschkeHojdisetal.2015, author = {Hager, Jonathan and Hentschke, Reinhard and Hojdis, Nils and Karimi-Varzaneh, Hossein Ali}, title = {Computer Simulation of Particle-Particle Interaction in a Model Polymer Nanocomposite}, series = {Macromolecules}, volume = {48}, journal = {Macromolecules}, number = {24}, issn = {1520-5835}, doi = {10.1021/acs.macromol.5b01864}, pages = {9039 -- 9049}, year = {2015}, language = {en} } @article{BaeckerBegingBisellietal.2009, author = {B{\"a}cker, Matthias and Beging, Stefan and Biselli, Manfred and Poghossian, Arshak and Wang, J. and Zang, Werner and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Concept for a solid-state multi-parameter sensor system for cell-culture monitoring}, series = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, journal = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0013-4686}, pages = {6107 -- 6112}, year = {2009}, language = {en} } @article{HaegerGrankinWagner2023, author = {Haeger, Gerrit and Grankin, Alina and Wagner, Michaela}, title = {Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology}, series = {Applied Research}, journal = {Applied Research}, number = {Early View}, publisher = {Wiley-VCH}, issn = {2702-4288}, doi = {10.1002/appl.202200106}, pages = {1 -- 15}, year = {2023}, abstract = {Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47\%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78\%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.}, language = {en} } @article{Scherer2006, author = {Scherer, Ulrich W.}, title = {Controlled ion track etching / J. George; M. Irkens ; S. Neumann ; U. W. Scherer ; A. Srivastava ; D. Sinha ; D. Fink}, series = {Radiation Effects and Defects in Solids. 161 (2006), H. 3}, journal = {Radiation Effects and Defects in Solids. 161 (2006), H. 3}, pages = {161 -- 175}, year = {2006}, language = {en} } @article{SelmerLukatelaKraussetal.1998, author = {Selmer, Thorsten and Lukatela, G. and Krauss, N. and Theis, K.}, title = {Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis / Lukatela, G. ; Krauss, N. ; Theis, K. ; Selmer, T. ; Gieselmann, V. ; Figura, K. von ; Saenger,}, series = {Biochemistry. 37 (1998), H. 11}, journal = {Biochemistry. 37 (1998), H. 11}, pages = {3654 -- 3664}, year = {1998}, language = {en} } @article{HemmerlingMerschenzQuackWunderlich1988, author = {Hemmerling, H.-J. and Merschenz-Quack, Angelika and Wunderlich, H.}, title = {Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988}, series = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, volume = {185}, journal = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, number = {H. 1-4}, issn = {2196-7105 (E-Books); 2194-4946 (Print)}, pages = {256}, year = {1988}, language = {en} } @article{LauthHoelderichWagenblast1995, author = {Lauth, Jakob and Hoelderich, W. and Wagenblast, G.}, title = {Crystalline zeolites containing indigo dyes}, series = {Zeolites. 15 (1995), H. 1}, journal = {Zeolites. 15 (1995), H. 1}, isbn = {0144-2449}, pages = {86}, year = {1995}, language = {en} } @article{BiselliNollJelineketal.2002, author = {Biselli, Manfred and Noll, Thomas and Jelinek, Nanni and Schmidt, Sebastian}, title = {Cultivation of Hematopoietic Stem and Progenitor Cells: biochemical Engineering Aspects / Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli und Christian Wandrey}, series = {Tools and Applications of Biochemical Engineering Science}, journal = {Tools and Applications of Biochemical Engineering Science}, publisher = {Springer}, address = {Berlin}, isbn = {3-540-42250-1}, pages = {111 -- 128}, year = {2002}, language = {en} } @article{BiselliHilbertNoll2001, author = {Biselli, Manfred and Hilbert, U. and Noll, T.}, title = {Cultivation of Human HCMV Specific Lymphocytes - An Example for Adoptive Immunotherapy / Hilbert, U. ; Biselli, M. ; Noll, T.}, series = {Animal cell technology : from target to market ; Tyl{\"o}sand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...}, journal = {Animal cell technology : from target to market ; Tyl{\"o}sand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...}, publisher = {Kluwer}, address = {Dordrecht}, isbn = {1-4020-0264-5}, pages = {558 -- 561}, year = {2001}, language = {en} } @article{Schnitzler2009, author = {Schnitzler, Thomas}, title = {Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)}, series = {Application notes / Sartorius stedim biotech}, journal = {Application notes / Sartorius stedim biotech}, pages = {1 -- 4}, year = {2009}, language = {en} } @article{HendersonMclaughlinScheeretal.2015, author = {Henderson, Colin J. and Mclaughlin, Lesley A. and Scheer, Nico and Stanley, Lesley A. and Wolf, C. Roland}, title = {Cytochrome b5 Is a Major Determinant of Human Cytochrome P450 CYP2D6 and CYP3A4 Activity In Vivo s}, series = {Molecular Pharmacology}, volume = {87}, journal = {Molecular Pharmacology}, number = {4}, publisher = {ASPET}, address = {Bethesda}, issn = {1521-0111}, doi = {10.1124/mol.114.097394}, pages = {733 -- 739}, year = {2015}, language = {en} } @article{FeuerriegelKloseSloboshaninetal.1994, author = {Feuerriegel, Uwe and Klose, W. and Sloboshanin, S. and Goebel, H. [u.a.]}, title = {Deactivation of a palladium-supported alumina catalyst by hydrogen sulfide during the oxidation of methane}, series = {Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10}, journal = {Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10}, isbn = {0743-74363}, pages = {3567 -- 3570}, year = {1994}, language = {en} } @article{ScheerKapelyukhRodeetal.2015, author = {Scheer, Nico and Kapelyukh, Yury and Rode, Anja and Oswald, Stefan and Busch, Diana and Mclaughlin, Lesley A. and Lin, De and Henderson, Colin J. and Wolf, C. Roland}, title = {Defining Human Pathways of Drug Metabolism In Vivo through the Development of a Multiple Humanized Mouse Model}, series = {Drug Metabolism and Disposition}, volume = {43}, journal = {Drug Metabolism and Disposition}, number = {11}, publisher = {ASPET}, address = {Bethesda}, issn = {1521-009x}, doi = {10.1124/dmd.115.065656}, pages = {1679 -- 1690}, year = {2015}, language = {en} } @article{KapelyukhHendersonScheeretal.2019, author = {Kapelyukh, Yury and Henderson, Colin James and Scheer, Nico and Rode, Anja and Wolf, Charles Roland}, title = {Defining the contribution of CYP1A1 and CYP1A2 to drug metabolism using humanized CYP1A1/1A2 and Cyp1a1/Cyp1a2 KO mice}, series = {Drug Metabolism and Disposition}, journal = {Drug Metabolism and Disposition}, number = {Early view}, doi = {10.1124/dmd.119.087718}, pages = {43 Seiten}, year = {2019}, language = {en} } @article{ScheerMclaughlinRodeetal.2014, author = {Scheer, Nico and Mclaughlin, Lesley A. and Rode, Anja and MacLeod, Alastair Kenneth and Henderson, Colin J. and Wolf, Roland C.}, title = {Deletion of thirty murine cytochrome P450 genes results in viable mice with compromised drug metabolism}, series = {Drug Metabolism and Disposition}, volume = {42}, journal = {Drug Metabolism and Disposition}, number = {6}, publisher = {ASPET}, address = {Bethesda, Md.}, issn = {1521-009X}, doi = {10.1124/dmd.114.057885}, pages = {1022 -- 1030}, year = {2014}, abstract = {In humans, 75\% of all drugs are metabolized by the cytochrome P450-dependent monooxygenase system. Enzymes encoded by the CYP2C, CYP2D, and CYP3A gene clusters account for ∼80\% of this activity. There are profound species differences in the multiplicity of cytochrome P450 enzymes, and the use of mouse models to predict pathways of drug metabolism is further complicated by overlapping substrate specificity between enzymes from different gene families. To establish the role of the hepatic and extrahepatic P450 system in drug and foreign chemical disposition, drug efficacy, and toxicity, we created a unique mouse model in which 30 cytochrome P450 genes from the Cyp2c, Cyp2d, and Cyp3a gene clusters have been deleted. Remarkably, despite a wide range of putative important endogenous functions, Cyp2c/2d/3a KO mice were viable and fertile, demonstrating that these genes have evolved primarily as detoxification enzymes. Although there was no overt phenotype, detailed examination showed Cyp2c/2d/3a KO mice had a smaller body size (15\%) and larger livers (20\%). Changes in hepatic morphology and a decreased blood glucose (30\%) were also noted. A five-drug cocktail of cytochrome P450 isozyme probe substrates were used to evaluate changes in drug pharmacokinetics; marked changes were observed in either the pharmacokinetics or metabolites formed from Cyp2c, Cyp2d, and Cyp3a substrates, whereas the metabolism of the Cyp1a substrate caffeine was unchanged. Thus, Cyp2c/2d/3a KO mice provide a powerful model to study the in vivo role of the P450 system in drug metabolism and efficacy, as well as in chemical toxicity.}, language = {en} } @article{PrielmeierRadkowitschLangetal.1986, author = {Prielmeier, Franz and Radkowitsch, H. and Lang, E. W. and L{\"u}demann, H.-D.}, title = {Density dependence of the molecular dynamics of fluid CH3F and CF3H studied by NMR / H. Radkowitsch, F. X. Prielmeier, E. W. Lang and H.-D. L{\"u}demann}, series = {Physica B+C. 139-140 (1986)}, journal = {Physica B+C. 139-140 (1986)}, isbn = {0921-4526}, pages = {96 -- 99}, year = {1986}, language = {en} } @article{MuesgenanntKoersPrevostPaulssenetal.2023, author = {Mues genannt Koers, Lucas and Prevost, David and Paulßen, Elisabeth and Hoehr, Cornelia}, title = {Density reduction effects on the production of [11C]CO2 in Nb-body targets on a medical cyclotron}, volume = {199}, number = {Art. 110911}, publisher = {Elsevier}, address = {Amsterdam}, doi = {10.1016/j.apradiso.2023.110911}, year = {2023}, abstract = {Medical isotope production of 11C is commonly performed in gaseous targets. The power deposition of the proton beam during the irradiation decreases the target density due to thermodynamic mixing and can cause an increase of penetration depth and divergence of the proton beam. In order to investigate the difference how the target-body length influences the operation conditions and the production yield, a 12 cm and a 22 cm Nb-target body containing N2/O2 gas were irradiated using a 13 MeV proton cyclotron. It was found that the density reduction has a large influence on the pressure rise during irradiation and the achievable radioactive yield. The saturation activity of [11C]CO2 for the long target (0.083 Ci/μA) is about 10\% higher than in the short target geometry (0.075 Ci/μA).}, language = {en} } @article{KotterRiekertWeyland1983, author = {Kotter, Michael and Riekert, L. and Weyland, F.}, title = {Deposition of ternary oxides as active components by impregnation of porous carriers}, series = {Preparation of Catalysts III : scientific bases for the preparation of heterogeneous catalysts ; proceedings of the Third International Symposium, Louvain-la-Neuve, September 6-9, 1982 / ed.: G. Poncelet ... - (Studies in surface science and catalysis ; 16)}, journal = {Preparation of Catalysts III : scientific bases for the preparation of heterogeneous catalysts ; proceedings of the Third International Symposium, Louvain-la-Neuve, September 6-9, 1982 / ed.: G. Poncelet ... - (Studies in surface science and catalysis ; 16)}, publisher = {Elsevier}, address = {Amsterdam [u.a.]}, isbn = {0-444-42184-X}, pages = {521 -- 530}, year = {1983}, language = {en} } @article{KalbeHoeckerBerndt1989, author = {Kalbe, Jochen and H{\"o}cker, Hartwig and Berndt, Heinz}, title = {Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters}, series = {Chromatographia}, volume = {28}, journal = {Chromatographia}, number = {3-4}, isbn = {0009-5893}, doi = {10.1007/BF02319646}, pages = {193 -- 196}, year = {1989}, language = {en} } @article{LeursMezoOehlschlaegeretal.2012, author = {Leurs, Ulrike and Mezo, Gabor and {\"O}hlschl{\"a}ger, Peter and Orban, Erika and Marquard, Andrea and Manea, Marilena}, title = {Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug-bioconjugates containing GnRH-III as a targeting moiety}, series = {Peptide Science}, volume = {98}, journal = {Peptide Science}, number = {1}, publisher = {Wiley}, address = {New York, NY}, issn = {1097-0282}, doi = {10.1002/bip.21640}, pages = {1 -- 10}, year = {2012}, abstract = {Bioconjugates containing the GnRH-III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of the bioconjugates would be the attachment of more than one chemotherapeutic agent to one GnRH-III molecule. Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing GnRH-III as a targeting moiety and daunorubicin as a chemotherapeutic agent. Two different drug design approaches were pursued. The first one was based on the bifunctional [4Lys]-GnRH-III (Glp-His-Trp-Lys-His-Asp-Trp-Lys-Pro-Gly-NH2) containing two lysine residues in positions 4 and 8, whose ϵ-amino groups were used for the coupling of daunorubicin. In the second drug design, the native GnRH-III (Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2) was used as a scaffold; an additional lysine residue was coupled to the ϵ-amino group of 8Lys in order to generate two free amino groups available for conjugation of daunorubicin. The in vitro stability/degradation of all synthesized compounds was investigated in human serum, as well as in the presence of rat liver lysosomal homogenate. Their cellular uptake was determined on human breast cancer cells and the cytostatic effect was evaluated on human breast, colon and prostate cancer cell lines. Compared with a monofunctional compound, both drug design approaches resulted in multifunctional bioconjugates with increased cytostatic effect.}, language = {en} } @article{SchmichEdererEbert1992, author = {Schmich, Peter and Ederer, Hanns J. and Ebert, Klaus H.}, title = {Detection and identification of free radicals in hydrocarbon pyrolysis by an iodine trapping method}, series = {Industrial \& Engineering Chemistry Research. 31 (1992), H. 1}, journal = {Industrial \& Engineering Chemistry Research. 31 (1992), H. 1}, isbn = {1520-5045}, pages = {29 -- 37}, year = {1992}, language = {en} } @article{WeldenSeverinsPoghossianetal.2022, author = {Welden, Melanie and Severins, Robin and Poghossian, Arshak and Wege, Christina and Bongaerts, Johannes and Siegert, Petra and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor}, series = {Chemosensors}, volume = {10}, journal = {Chemosensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors10060218}, pages = {Artikel 218}, year = {2022}, abstract = {Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte-insulator-semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin-streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage-current, capacitance-voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.}, language = {en} } @article{MolinnusBaeckerSiegertetal.2015, author = {Molinnus, Denise and B{\"a}cker, Matthias and Siegert, Petra and Willenberg, H. and Poghossian, Arshak and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Detection of Adrenaline Based on Substrate Recycling Amplification}, series = {Procedia Engineering}, volume = {120}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2015.08.708}, pages = {540 -- 543}, year = {2015}, abstract = {An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.}, language = {en} } @article{TippkoetterDeterdingUlber2008, author = {Tippk{\"o}tter, Nils and Deterding, A. and Ulber, Roland}, title = {Determination of acetic acid in fermentation broth by gas-diffusion technique}, series = {Engineering in Life Sciences}, volume = {8}, journal = {Engineering in Life Sciences}, number = {1, Special Issue: Technical Systems for the Use in Life Sciences}, doi = {10.1002/elsc.200820227}, pages = {62 -- 67}, year = {2008}, abstract = {Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5-5.0 g/L acetic acid with a relative standard deviation of <5 \% was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.}, language = {en} }